5-Hydroxymethylome in Circulating Cell-free DNA as A Potential Biomarker for Non-small-cell Lung Cancer

查看全文 作　　者：Ji [1,2]Zhang;Xiao [3]Han;Chunchun [3]Gao;Yurong [4]Xing;Zheng [5]Qi;Ruijuan [1,2]Liu;Yueqin [1,2]Wang;Xiaojian [1,2]Zhang;Yun-Gui [3,6,7]Yang;Xiangnan [8]Li;Baofa [3,6,7]Sun;Xin [1,2]Tian

机构地区：[1]Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University;[2]Henan Key Laboratory of Precision Clinical Pharmacy, Zhengzhou University;[3]Sino-Danish College, University of Chinese Academy of Sciences;[4]Physical Examination Center, The First Affiliated Hospital of Zhengzhou University;[5]Department of Anesthesiology, The First Affiliated Hospital of Zhengzhou University;[6]CAS Key Laboratory of Genomic and Precision Medicine, School of Future Technology, Beijing Institute of Genomics,Chinese Academy of Sciences;[7]Institute of Stem Cell and Regeneration, Chinese Academy of Sciences;[8]Department of Thoracic Surgery, The First Affiliated Hospital of Zhengzhou University

出　　处：《Genomics, Proteomics & Bioinformatics》索引2018年第16卷第3期,共13页

基　　金：supported by grants from the Ministry of Science and Technology of China (Grant No. 2016YFC0900300);National Natural Science Foundation of China (Grant Nos. 31670895, U1504831, 31430022, 31670824, 31400672, and 81703598);Major Science and Technology Project of Henan Province (Grant No. 161100310100);Strategic Priority Research Program of the Chinese Academy of Sciences (Grant No. XDB14030300);Foundation for University Young Key Teacher of Henan Province (Grant No. 2015GGJS-162);Science and Technology Project of Henan Province (Grant No.162102310199), China 摘　　要：Non-small-cell lung cancer(NSCLC), the most common type of lung cancer accounting for 85% of the cases, is often diagnosed at advanced stages owing to the lack of efficient early diagnostic tools. 5-Hydroxymethylcytosine(5 hmC) signatures in circulating cell-free DNA(cfDNA) that carries the cancer-specific epigenetic patterns may represent the valuable biomarkers for discriminating tumor and healthy individuals, and thus could be potentially useful for NSCLC diagnosis. Here,we employed a sensitive and reliable method to map genome-wide 5 hmC in the cfDNA of Chinese NSCLC patients and detected a significant 5 hmC gain in both the gene bodies and promoter regions in the blood samples from tumor patients compared with healthy controls. Specifically, we identified six potential biomarkers from 66 patients and 67 healthy controls(mean decrease accuracy>3.2, P < 3.68E-19) using machine-learning-based tumor classifiers with high accuracy. Thus,the unique signature of 5 hmC in tumor patient's cfDNA identified in our study may provide valuable information in facilitating the development of new diagnostic and therapeutic modalities for NSCLC. 关键词：DNA 癌症房间传播肺肿瘤病区域检测控制相

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5-Hydroxymethylome in Circulating Cell-free DNA as A Potential Biomarker for Non-small-cell Lung Cancer

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