Long noncoding RNA RP4 functions as a competing endogenous RNA through miR-7-5p sponge activity in colorectal cancer

查看全文 作　　者：Mu-Lin [1]Liu;Qiao [2]Zhang;Xiao [3]Yuan;Long [1]Jin;Li-Li [1]Wang;Tao-Tao [1]Fang;Wen-Bin [3]Wang

机构地区：[1]Department of Gastrointestinal Surgery, the First Affiliated Hospital of Bengbu Medical College;[2]Department of General Surgery, the First Affiliated Hospital of Xinxiang Medical University;[3]Department of General Surgery, the Fourth Affiliated Hospital of Anhui Medical University

出　　处：《World Journal of Gastroenterology》索引2018年第24卷第9期,共9页

基　　金：Supported by Scientific Research Foundation of Anhui Education Department,No.KJ2017A219 to Liu ML;Scientific Research Foundation of Academic Leader of Anhui Province,No.2016H105 to Liu ML;Education Talent Foundation of Universities of Anhui Education Department,No.gxbj ZD2016070 to Liu ML;National Natural Science Foundation of China,No.81500373 to Wang WB;Natural Science Foundation of Anhui Province,No.1608085MH193 to Wang WB 摘　　要：AIM To investigate the role of long noncoding RNA(lnc RNA) RP4 in colorectal cancer.METHODS Lentivirus-mediated lnc RNA RP4 overexpression and knockdown were performed in the colorectal cancer cell line SW480. Cell proliferation, tumor growth, and early apoptosis were evaluated by a cell counting kit-8 assay, an in vivo xenograft tumor model, and annexin V/propidium iodide staining, respectively. Analysis of the lnc RNA RP4 mechanism involved assessment of the association of its expression with mi R-7-5 p and the SH3 GLB1 gene. Western blot analysis was also performed to assess the effect of lnc RNA RP4 on the autophagy-mediated cell death pathway and phosphatidylinositol-3-kinase(PI3 K)/Akt signaling.RESULTS Cell proliferation, tumor growth, and early apoptosis in SW480 cells were negatively regulated by lnc RNA RP4. Functional experiments indicated that lnc RNA RP4 directly upregulated SH3 GLB1 expression by acting as a competing endogenous RNA(ce RNA) for mi R-7-5 p. This interaction led to activation of the autophagy-mediated cell death pathway and de-repression of PI3 K and Akt phosphorylation in colorectal cancer cells in vivo.CONCLUSION Our results demonstrated that lnc RNA RP4 is a ce RNA that plays an important role in the pathogenesis of colorectal cancer, and could be a potential therapeutic target for colorectal cancer treatment. 关键词：COLORECTAL cancer LONG noncoding RNA RP4 SH3GLB1 miR-7-5p competing ENDOGENOUS RNA

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Long noncoding RNA RP4 functions as a competing endogenous RNA through miR-7-5p sponge activity in colorectal cancer

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