Content Determination of Quercetin in Ferment of Ginkgo biloba L. Leaves by HPLC

查看全文 作　　者：Dengfeng [1]ZOU;Hua [1]ZHU;Junying [1]RUAN;Xiaohua [1]WANG

机构地区：[1]School of Pharmacy,Guilin Medical University

出　　处：《Medicinal Plant》索引2018年第9卷第6期,共4页

基　　金：Supported by Project of Guilin Science and Technology Bureau(20100305);Guangxi Collaborative Innovation Center:Zhuang Yao Medicine Collaborative Innovation Center(Gui 2013[20]);Guangxi Traditional Chinese Medicine Science and Technology Project(GZMZ1202) 摘　　要：[Objectives] This study aimed to determine the content of quercetin in ferment of Ginkgo biloba L.leaves.[Methods]Bacillus licheniformis was selected for solid-state fermentation of G.biloba leaf powder,and the content of quercetin in ferment of G.biloba leaves was determined by reversed-phase high-performance liquid chromatography.First,the flavonoid glycosides were extracted with methanol.Then,the flavonoid glycosides were hydrolyzed with hydrochloric acid to prepare the test solution.The chromatographic conditions were as follows:Platisil ODS C_(18) column(150 mm × 4.6 mm,5 μm);V_(methonal)∶V_(water)(0.4% phosphoric acid solution) =55∶45;flow rate of 1 m L/min;Shimadzu UV detector;detection wavelength of 360 nm.[Results] Quercetin was used as a reference substance.In the range of 0.002 6-0.036 0 g/L,there was a good linear relationship,with correlation coefficient of 0.999 8 and RSD of 1.26%.[Conclusions] This method is simple,easy to operate,accurate,and reproducible.It is suitable for the determination of quercetin content in G.biloba leaves. 关键词：GINKGO biloba L.leaves MICROBIAL FERMENTATION High-performance liquid chromatography Content determination

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Content Determination of Quercetin in Ferment of Ginkgo biloba L. Leaves by HPLC

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