Rapid Creation of New Photoperiod-/Thermo-Sensitive Genic Male-Sterile Rice Materials by CRISPR/Cas9 System

查看全文 作　　者：SHEN [1]Lan;DONG [1]Guojun;ZHANG [1]Yu;HU [1]Guocheng;ZHANG [1]Qiang;HU [1]Guanglian;XU [1]Bo;REN [1]Deyong;HU [1]Jiang;ZHU [1]Li;GAO [1]Zhenyu;ZHANG [1]Guangheng;GUO [1]Longbiao;ZENG [1]Dali;QIAN [1]Qian

机构地区：[1]State Key Laboratory of Rice Biology/China National Rice Research Institute, Chinese Academy of Agricultural Sciences

出　　处：《Rice science》索引2019年第26卷第2期,共5页

基　　金：supported by the Central Public-Interest Scientific Institution Basal Research Fund of China National Rice Research Institute (Grant No. 2017RG001-4) 摘　　要：Rice is an important food crop in China, and the development of hybrid rice is a crucial way to increase grain yield. The creation of dual-purpose nuclear-sterile lines for two-line hybrid breeding has become vital for commercial rice breeding. We constructed the pC1300-2 x35 S::Cas9-sgRNA^(PTGMS2-1) expression vector for editing the male fertility gene PTGMS2-1 in two widely compatible rice varieties, 93-11 and Huazhan, by using the CRISPR/Cas9 system. We obtained the marker-free photoperiod-/thermo-sensitive genic male-sterile(P/TGMS) lines in T_1 generation. According to the experiments in phytotron with four temperature and photoperiod treatments, we found the temperature is the main factor for restoring the pollen fertility of ptgms2-1 mutants in 93-11 and Huazhan, and the photoperiod also has some effects on pollen fertility in two different rice backgrounds. The application of cultivating new male-sterile lines by genome editing system will significantly accelerate the rice breeding process. 关键词：RICE CREATION MARKER-FREE

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Rapid Creation of New Photoperiod-/Thermo-Sensitive Genic Male-Sterile Rice Materials by CRISPR/Cas9 System

参考文献(13)

引证文献(4)

耦合文献(192)

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