Hierarchically spacing DNA probes on bio-based nanocrystal for spatial detection requirements

查看全文 作　　者：Lin [1,2]Gan;Ya [1,2]Wang;Meng [1,2]Zhang;Xuehuan [1,2]Xia;Jin [1,2]Huang

机构地区：[1]Key Laboratory of Luminescence and Real-Time Analytical Chemistry,Ministry of Education,School of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China;[2]Chongqing Key Laboratory of Soft-Matter Material Chemistry and Function Manufacturing,Southwest University,Chongqing 400715,China

出　　处：《Science Bulletin》索引2019年第64卷第13期,共7页

基　　金：financially supported by the National Natural Science Foundation of China (51603171 and 51373131);Talent Project of Southwest University (SWU115034);Fundamental Research Funds for the Central Universities (XDJK2016C032);Key Laboratory of Polymeric Composite & Functional Materials of Ministry of Education (PCFM201605);Hubei Key Laboratory of Advanced Textile Materials & Application (Fzxcl2017003) 摘　　要：Sterically spacing and locating functional matters at the nanoscale exert critical effects on their application,especially for the fluorescence probes whose aggregation causes emission quenching.Here we achieved a hierarchical spacing strategy of DNA fluorescence probes for ion detection via locating them separately on rod-like cellulose nanocrystals(CNCs)and further isolating CNCs by pre-grafting long molecular chains.Controlling chemical structure of CNC and location degree could adjust the interspace of DNA probes(with a molecular length of ca.3.6 nm)in a range of 3.5-6.5 nm with a gradient about 0.2 nm.A length up to micrometer scale of the CNC nanorods was necessary to provide DNA probes with well-separated grafting locations and enough freedom,which brought a vast linear detection range from 10 nmol/L to 5 μmol/L of Hg^2+ concentration.The abundant reactive sites on CNC allowed a grafting pre-location of poly(tert-butyl acrylate)(PtBA)to promote the isolation of DNA probes.Controlled radical polymerization was employed to adj ust the length of PtBA molecular chains,which increased the linear sensitivity coefficient of Hg^2+ detection by ca.2.5 times.This hierarchical nanoscale spacing concept based on chemical design can hopefully cond uce to the development of biosensor and medical diagnosis.A hierarchical spacing strategy was applied to separate DNA fluorescent probes on CNCs and detect ion concentration linearly.The first-level spacing was to locate probes uniformly on CNCs,obtaining a wide linear range;and the second-level spacing was to isolate CNCs with polymer,obtaining an increased linear coefficient. 关键词：CELLULOSE nanocrystal DNA fluorescence PROBE STERIC SPACING Surface modification MERCURY ion DETECTION

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Hierarchically spacing DNA probes on bio-based nanocrystal for spatial detection requirements

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