Silicon Nanowire-Based Methodology for Quantifying Single Cell Traction Force

查看全文 作　　者：NIU Yong-[1,2]shan;YAN [1]Ling;DAI [1]Ming;FAN Yu-[1,2]bo;LI [1,2]Zhou 机构地区：[1]School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China;[2]Key Lab for Biomechanics and Mechanobiology of Ministry of Education, Beihang University, Beijing 100191, China 出　　处：《Chinese Journal of Biomedical Engineering(English Edition)》索引2018年第27卷第4期,共7页 基　　金：New Teachers’Fund for Doctor Stations,Ministry of Education;grant number:20111102120038 摘　　要：In this paper, we present a new method, a silicon-nanowire-array based technique for quantifying the mechanical behavior of single cells, representing three distinct groups: normal mammalian cells, benign cells (L929) and malignant cells (HeLa). By culturing the cells on top of NW arrays, the maximum traction forces of different cells have been measured by quantitatively analyzing the bending of the nanowires. The elastic modulus of the as-fabricated Si-NW arrays was first measured before cell culturing. Finite element (FEM) simulations were carried out in order to derive the relationship between the applied transverse force and the corresponding tip displacement for a Si-NW. Our study is likely important for studying the mechanical properties of single cells and their migration characteristics, possibly providing a new cellular level diagnostic technique. 关 键 词：silicon NANOWIRE array cell TRACTION FORCE FINITE element simulation CELLULAR mechanical properties