RAVL1 Activates IDD3 to Negatively Regulate Rice Resistance to Sheath Blight Disease

查看全文 作　　者：SUN [1]Qian;YANG [1]Shuo;GUO [2]Xiaofan;WANG [1]Siting;JIA [1]Xintong;LI [3]Shuang;XUAN [1]Yuanhu

机构地区：[1]College of Plant Protection,Shenyang Agricultural University,Shenyang 110866,China;[2]School of Life Science and Technology,Hubei Engineering University,Xiaogan 432000,China;[3]Shaanxi Key Laboratory of Chinese Jujube/College of Life,Yan’an University,Yan’an 716000,China

出　　处：《Rice science》索引2021年第28卷第2期,共10页

基　　金：the Science and Technology Innovation Talents of Shenyang,China(Grant No.RC190489). 摘　　要：Sheath blight disease (ShB) has a severe impact on the production of rice. ABI3/VP1-like 1(RAVL1) negatively regulated the rice defense mechanism against ShB, however, this regulatorymechanism is not clearly understood. In this study, we identified that indeterminate domain 3 (IDD3) waspositively regulated by RAVL1. Further, chromatin immunoprecipitation (ChIP) assay, yeast one-hybridassay and transient expression assay indicated a direct binding between RAVL1 and the IDD3 promoterregion. IDD3 was ubiquitously expressed in different tissues and at different stages, and its expressionwas significantly enhanced by Rhizoctonia solani infection. IDD3 exhibited transcription activation activityin yeast and IDD3-GFP was found to be localized in the nucleus. IDD3 mutants exhibited no significantdifferences in response to ShB, while IDD3 overexpressors were more susceptible to ShB compared withwild type (WT) plants. Furthermore, IDD3 repressors were less susceptible to R. solani than WT plants.Interestingly, the expression of brassinosteroid-related genes (D2, D11 and BRI1) was lower in IDD3repressors and higher in IDD3 overexpressors compared with WT. However, the ChIP assay revealedthat IDD3 did not directly bind to the D2 and D11 promoters. Overexpression of IDD3 in BRI1 mutantd61-1 inhibited the activity of IDD3, reducing its susceptibility to ShB compared with IDD3 overexpressorand WT plants, indicating that IDD3 negatively regulated the rice defense mechanism against ShB by activatingthe BR signaling pathway. Thus, our analyses provided information to enhance the understanding of therice defense mechanism against ShB. 关键词：sheath blight disease AB13/VP1-like 1 indeterminate domain 3 RICE promoter

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RAVL1 Activates IDD3 to Negatively Regulate Rice Resistance to Sheath Blight Disease

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