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Effects of brewers' spent grain protein hydrolysates on gas production, ruminal fermentation characteristics, microbial protein synthesis and microbial community in an artificial rumen fed a high grain diet

查看全文 作　　者：Tao [1,2,3]Ran;Long [1]Jin;Ranithri [4]Abeynayake;Atef Mohamed [1,5]Saleem;Xiumin [1,2]Zhang;Dongyan [3]Niu;Lingyun [4]Chen;Wenzhu [1]Yang 高影响力作者 机构地区：[1]Agriculture and Agri-Food of Canada,Lethbridge Research and Development Centre,Lethbridge,AB T1J 4B1,Canada;[2]Key Laboratory for Agro-Ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,Hunan,China;[3]College of Veterinary Medicine,University of Calgary,2500 University Dr.NW,Calgary,AB T2N 1N4,Canada;[4]Department of Agricultural,Food and Nutritional Science,University of Alberta,Edmonton T6G 2P5,Canada;[5]Department of Animal and Poultry Production,Faculty of Agriculture,South Valley University,Qena 83523,Egypt.高影响力机构 出　　处：《Journal of Animal Science and Biotechnology》索引2021年第12卷第1期,共14页高影响力期刊 基　　金：financially supported by the Agriculture and Agri-Food Canada(AAFC) Growing forward program (GF2#1542)。 摘　　要：Background: Brewers' spent grain(BSG) typically contains 20% – 29% crude protein(CP) with high concentrations of glutamine, proline and hydrophobic and non-polar amino acid, making it an ideal material for producing valueadded products like bioactive peptides which have antioxidant properties. For this study, protein was extracted from BSG, hydrolyzed with 1% alcalase and flavourzyme, with the generated protein hydrolysates(AlcH and FlaH)showing antioxidant activities. This study evaluated the effects of AlcH and FlaH on gas production, ruminal fermentation characteristics, nutrient disappearance, microbial protein synthesis and microbial community using an artificial rumen system(RUSITEC) fed a high-grain diet.Results: As compared to the control of grain only, supplementation of FlaH decreased(P < 0.01) disappearances of dry matter(DM), organic matter(OM), CP and starch, without affecting fibre disappearances;while AlcH had no effect on nutrient disappearance. Neither AlcH nor FlaH affected gas production or VFA profiles, however they increased(P < 0.01) NH_3-N and decreased(P < 0.01) H_2 production. Supplementation of FlaH decreased(P < 0.01)the percentage of CH_4 in total gas and dissolved-CH_4(dCH_4) in dissolved gas. Addition of monensin reduced(P < 0.01) disappearance of nutrients, improved fermentation efficiency and reduced CH_4 and H_2 emissions.Total microbial nitrogen production was decreased(P < 0.05) but the proportion of feed particle associated(FPA) bacteria was increased with FlaH and monensin supplementation. Numbers of OTUs and Shannon diversity indices of FPA microbial community were unaffected by AlcH and FlaH;whereas both indices were reduced(P < 0.05) by monensin. Taxonomic analysis revealed no effect of AlcH and FlaH on the relative abundance(RA) of bacteria at phylum level, whereas monensin reduced(P < 0.05) the RA of Firmicutes and Bacteroidetes and enhanced Proteobacteria. Supplementation of FlaH enhanced(P < 0.05) the RA of genus Prevotella, reduced Selenomonas, Shuttleworthia, Bifidobacterium and Dialister as compared to control;monensin reduced(P < 0.05) RA of genus Prevotella but enhaced Succinivibrio.Conclusions: The supplementation of FlaH in high-grain diets may potentially protect CP and starch from ruminal degradation, without adversely affecting fibre degradation and VFA profiles. It also showed promising effects on reducing CH_4 production by suppressing H_2 production. Protein enzymatic hydrolysates from BSG using flavourzyme showed potential application to high value-added bio-products. 关 键 词：Antioxidant peptide Brewers'spent grain Fermentation Hydrogen production Methane production Protein hydrolysates RUSITEC
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