Bulk-like endocytosis plays an important role in the recycling of insulin granules in pancreatic beta cells

查看全文 作　　者：Du [1,4]Wen;Yanhong [1,4]Xue;Kuo [3]Liang;Tianyi [2]Yuan;Jingze [1]Lu;Wei [1,4]Zhao;Tao [1]Xu;Liangyi [2]Chen 机构地区：[1]National Key Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China;[2]Lab of Cell Secretion and Metabolism,Institute of Molecular Medicine,Peking University and National Center for Nanoscience and Technology,Beijing,100871,China;[3]Department of General Surgery,Xuanwu Hospital,Capital Medical University,Beijing 100053,China;[4]Graduate School of the Chinese Academy of Sciences,Beijing 100864,China 出　　处：《Protein & Cell》索引2012年第3卷第8期,共9页 基　　金：supported by a grant from the National Natural Science Foundation of China(Grant No.30871225);a grant from Beijing Municipal Science and Technology Commission(Grant No.7121008);a grant from the Ministry of Science and Technology(Grant No.SQ2011SF11B01041);the fund from The Key Construction Program of the National“985”Project from the Department of Education of China to Peking University. 摘　　要：Although bulk endocytosis has been found in a number of neuronal and endocrine cells,the molecular mechanism and physiological function of bulk endocytosis remain elusive.In pancreatic beta cells,we have observed bulk-like endocytosis evoked both by flash photolysis and trains of depolarization.Bulk-like endocytosis is a clathrin-independent process that is facilitated by enhanced extracellular Ca^(2+) entry and suppressed by the inhibition of dynamin function.Moreover,defects in bulklike endocytosis are accompanied by hyperinsulinemia in primary beta cells dissociated from diabetic KKAy mice,which suggests that bulk-like endocytosis plays an important role in maintaining the exo-endocytosis balance and beta cell secretory capability. 关 键 词：bulk-like endocytosis clathrin-independent endocytosis DYNAMIN diabetic KKAy mice