Real-time simultaneous recording of electrophysiological activities and dopamine overflow in the deep brain nuclei of a non-human primate with Parkinson’s disease using nano-based microelectrode arrays

查看全文 作　　者：Song [1,2]Zhang;Yilin [1,2]Song;Mixia [1,2]Wang;Guihua [1,2]Xiao;Fei [1,2]Gao;Ziyue [1,2]Li;Guoxian [3]Tao;Ping [4]Zhuang;Feng [3,4]Yue;Piu [4]Chan;Xinxia [1,2]Cai

机构地区：[1]State Key Laboratory of Transducer Technology,Institute of Electronics,Chinese Academy of Sciences,Beijing 100190,China;[2]University of Chinese Academy of Sciences,Beijing 100190,China;[3]Wincon TheraCells Biotechnologies Co.,Ltd.,Nanning 530002,China;[4]Department of Neurobiology,Xuanwu Hospital,Capital Medical University,The Beijing Key Laboratory of Parkinson's Disease,Beijing 100053,China

出　　处：《Microsystems & Nanoengineering》索引2018年第4卷第1期,共9页

基　　金：This work was sponsored by the NSFC(Grant Nos.61527815,31500800,61501426,61471342);the National Key Research and Development Program(Grant No.2017YFA0205900);the Beijing Science and Technology Plan(Grant Nos.Z141100000214002,Z1161100004916001);the National Science and Technology Major Project(2014CB744600);the Key Programs of the Chinese Academy of Sciences(Grant Nos.KJZD-EW-L11-2,QYZDJ-SSW-SYS015). 摘　　要：Parkinson’s disease(PD)is characterized by a progressive degeneration of nigrostriatal dopaminergic neurons.The precise mechanisms are still unknown.Since the neuronal communications are inherently electrical and chemical in nature,dual-mode detection of PD-related neuroelectrical and neurochemical information is essential for PD research.Subthalamic nucleus(STN)highfrequency stimulation(HFS)can improve most symptoms of PD patients and decrease the dosage of antiparkinsonian drugs.The mechanism of STN-HFS for PD still remains elusive.In this study,a silicon-based dual-mode microelectrode array(MEA)probe was designed and fabricated,and systematic dual-mode detection methods were established.The recording sites were modified using Pt nanoparticles and Nafion to improve the signal-to-noise(SNR)ratio.To evaluate its applicability to PD research,in vivo electrophysiological and electrochemical detection was performed in normal and hemiparkinsonian models,respectively.Through comparison of the dual-mode signals,we demonstrated the following in a PD monkey:(1)the maximum dopamine concentration in the striatum decreased by 90%;(2)the spike firing frequency increased significantly,especially in the region of the cortex;(3)the spectrogram analysis showed that much power existed in the 0–10 Hz frequency band;and(4)following repeated subthalamic nucleus high-frequency stimulation trials,the level of DA in the striatum increased by 16.5μM,which led to a better elucidation of the mechanism of HFS.The dual-mode MEA probe was demonstrated to be an effective tool for the study of neurological disorders. 关键词：dual mode implantable MEA non-human primate Parkinson's disease STN-HFS

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Real-time simultaneous recording of electrophysiological activities and dopamine overflow in the deep brain nuclei of a non-human primate with Parkinson’s disease using nano-based microelectrode arrays

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