L-Selenocystine induce HepG2 cells apoptosis through ROS-mediated signaling pathways

查看全文 作　　者：HAIYANG [1,2]CHEN;JINGYAO [1]SU;DANYANG [1]CHEN;YUYE [1]DU;RUILIN [1]ZHENG;QINGLIN [2]DENG;QIANQIAN [3]DU;BING [1]ZHU;YINGHUA [1]LI 机构地区：[1]Center Laboratory,Guangzhou Women and Children’s Medical Center,Guangzhou Medical University,Guangzhou,China;[2]Nanfang Hospital,Southern Medical University,Guangzhou,China;[3]Department of Laboratory Medicine,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou,China 出　　处：《BIOCELL》索引2022年第46卷第10期,共7页 基　　金：This work was supported by the fund from Open Project of Guangdong Key Laboratory of Marine Materia Medica(LMM2020-7);the fund from Guangzhou Institute of Pediatrics/Guangzhou Women and Children’s Medical Center(Nos.Pre-NSFC-2019-016,YIP-2019-059,IP-2019-019);the Project of Traditional Chinese Medicine Bureau of Guangdong Province(No.20192075);the technology planning projects of Guangdong province(No.202102010202);The Guangdong Natural Science Foundation(2020A1515110648);the Open Fund of Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications(2020A03). 摘　　要：At present,Hepatocarcinoma is one of the main causes of tumor related death all over the world.However,there are still many clinical restrictions on the treatment of liver cancer.Recently,L-Selenocystine has been shown to be a novel treatment for tumors,especially human glioma cells.But,the mechanism of L-Selenocystine against hepatocellular carcinoma remains unclear.Therefore,the main objective of this study was to investigate the effects of L-Selenocystine on HepG2 cell proliferation and activation of reactive oxygen species(ROS)mediated signaling pathway.L-Selenocystine can significantly inhibit HepG2 cell proliferation by activating caspase-3 and cleaving PARP to induce apoptosis.Moreover,the excessive production of ROS and the influence of Bax signaling pathway which can promote cell apoptosis are key factors for L-Selenocystine to induce HepG2 cell apoptosis.Therefore,the date of this study suggest that ROS mediated signal transduction mechanism may provide certain reference significance for L-Selenocystine induced HepG2 cell apoptosis. 关 键 词：SELENIUM APOPTOSIS L-Selenocystine Anticancer activity ROS