Vitamin B1 THIAMIN REQUIRING1 synthase mediates the maintenance of chloroplast function by regulating sugar and fatty acid metabolism in rice

查看全文 作　　者：Yanshen [1]Nie;Li [1]Yu;Lianlian [1]Mao;Wenxuan [1]Zou;Xiufeng [1]Zhang;Jie [1]Zhao

机构地区：[1]State Key Laboratory of Hybrid Rice,College of Life Sciences,Wuhan University,Wuhan 430072,China

出　　处：《Journal of Integrative Plant Biology》索引2022年第64卷第8期,共21页

基　　金：supported by the National Natural Science Foundation of China(32170337,31870303)。 摘　　要：Vitamin B(VB1),including thiamin,thiamin monophosphate(TMP),and thiamin pyrophosphate(TPP),is an essential micronutrient for all living organisms.Nevertheless,the precise function of VB1 in rice remains unclear.Here,we described a VB1 auxotrophic mutant,chlorotic lethal seedling(cles)from the mutation of OsTH1,which displayed collapsed chloroplast membrane system and decreased pigment content.OsTH1 encoded a phosphomethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase,and was expressed in various tissues,especially in seedlings,leaves,and young panicles.The VB1 content in cles was markedly reduced,despite an increase in the expression of VB1 synthesis genes.The decreased TPP content affected the tricarboxylic acid cycle,pentose phosphate pathway,and de novo fatty acid synthesis,leading to a reduction in fatty acids(C16:0 and C18:0)and sugars(sucrose and glucose)of cles.Additionally,irregular expression of chloroplast membrane synthesis genes led to membrane collapse.We also found that alternative splicing and translation allowed OsTH1 to be localized to both chloroplast and cytosol.Our study revealed that OsTH1 was an essential enzyme in VB1 biosynthesis and played crucial roles in seedling growth and development by participating in fatty acid and sugar metabolism,providing new perspectives on VB1 function in rice. 关键词：CHLOROPLAST fatty acid metabolism RICE sugar metabolism THIAMIN REQUIRING1 vitamin B1

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Vitamin B1 THIAMIN REQUIRING1 synthase mediates the maintenance of chloroplast function by regulating sugar and fatty acid metabolism in rice

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