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Blockage of ATPase-mediated energy supply inducing metabolic disturbances in algal cells under silver nanoparticles stress

查看全文 作　　者：Ruohua [1]Qu;Mi [1]Chen;Jingfu [2]Liu;Qiting [1]Xie;Na [1]Liu;Fei [1]Ge 高影响力作者 机构地区：[1]Department of Environment,College of Environment and Resources,Xiangtan University,Xiangtan 411105,China;[2]State Key Laboratory of Environmental Chemistry and Ecotoxicology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China高影响力机构 出　　处：《Journal of Environmental Sciences》索引2023年第9期,共10页高影响力期刊 基　　金：supported by the National Natural Science Foundation of China(Nos.21577117 and 22076160);the Postgraduate Scientific Research Innovation Project of Hunan Province(No.CX20210521). 摘　　要：Adenosine triphosphate(ATP)generation of aquatic organisms is often subject to nanoparticles(NPs)stress,involving extensive reprogramming of gene expression and changes in enzyme activity accompanied by metabolic disturbances.However,little is known about the mechanism of energy supply by ATP to regulate the metabolism of aquatic organisms under NPs stress.Here,we selected extensively existing silver nanoparticles(AgNPs)to investigate their implications on ATP generation and relevant metabolic pathways in alga(Chlorella vulgaris).Results showed that ATP content significantly decreased by 94.2%of the control(without AgNPs)in the algal cells at 0.20 mg/L AgNPs,which was mainly attributed to the reduction of chloroplast ATPase activity(81.4%)and the downregulation of ATPase-coding genes atpB and atpH(74.5%-82.8%)in chloroplast.Molecular dynamics simulations demonstrated that AgNPs competed with the binding sites of substrates adenosine diphosphate and inorganic phosphate by forming a stable complex with ATPase subunit beta,potentially resulting in the reduced binding efficiency of substrates.Furthermore,metabolomics analysis proved that the ATP content positively correlated with the content of most differential metabolites such as D-talose,myo-inositol,and L-allothreonine.AgNPs remarkably inhibited ATPinvolving metabolic pathways,including inositol phosphate metabolism,phosphatidylinositol signaling system,glycerophospholipid metabolism,aminoacyl-tRNA biosynthesis,and glutathione metabolism.These results could provide a deep understanding of energy supply in regulating metabolic disturbances under NPs stress. 关 键 词：Adenosine triphosphate Metabolomics Molecular dynamics simulations Nanoparticles ALGAE
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