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| 1 | Heterologous biosynthesis of natural product naringenin by co-culture engineering显示文摘Co-culture engineering is an emerging approach for microbial biosynthesis of a variety of biochemicals.In this study,E.coli-E.coli co-cultures were developed for heterologous biosynthesis of the natural product naringenin.The co-cultures were composed of two independent E.coli strains dedicated to functional expression of different portions of the biosynthetic pathway,respectively.The co-culture biosynthesis was optimized by investigating the effect of carbon source,E.coli strain selection,timing of IPTG induction and the inoculation ratio between the co-culture strains.Compared with the monoculture strategy,the utilization of the designed co-cultures significantly improved the naringenin production,largely due to the reduction of metabolic stress,employment of proper hosts for improving pathway enzyme activities,and flexible adjustment of the relative biosynthetic strength between the coculture strains.The findings of this study extend the applicability of co-culture engineering in complex natural product biosynthesis. | Vijaydev Ganesan Zhenghong Li Xiaonan Wang Haoran Zhang | 2017 | Synthetic and Systems Biotechnology2017，2，3： | 13 |
| 2 | Engineering of Yarrowia lipolytica for production of astaxanthin显示文摘Astaxanthin is a red-colored carotenoid,used as food and feed additive.Astaxanthin is mainly produced by chemical synthesis,however,the process is expensive and synthetic astaxanthin is not approved for human consumption.In this study,we engineered the oleaginous yeast Yarrowia lipolytica for de novo production of astaxanthin by fermentation.First,we screened 12 different Y.lipolytica isolates for β-carotene production by introducing two genes for β-carotene biosynthesis:bi-functional phytoene synthase/lycopene cyclase(crtYB)and phytoene desaturase(crtI)from the red yeast Xanthophyllomyces dendrorhous.The best strain produced 31.1±0.5 mg/L β-carotene.Next,we optimized the activities of 3-hydroxy-3-methylglutaryl-coenzyme A reductase(HMG1)and geranylgeranyl diphosphate synthase(GGS1/crtE)in the best producing strain and obtained 453.9±20.2 mg/L β-carotene.Additional downregulation of the competing squalene synthase SQS1 increased the β-carotene titer to 797.1±57.2 mg/L.Then we introduced β-carotene ketolase(crtW)from Paracoccus sp.N81106 and hydroxylase(crtZ)from Pantoea ananatis to convert β-carotene into astaxanthin.The constructed strain accumulated 10.4±0.5 mg/L of astaxanthin but also accumulated astaxanthin biosynthesis intermediates,5.7±0.5 mg/L canthaxanthin,and 35.3±1.8 mg/L echinenone.Finally,we optimized the copy numbers of crtZ and crtW to obtain 3.5 mg/g DCW(54.6 mg/L)of astaxanthin in a microtiter plate cultivation.Our study for the first time reports engineering of Y.lipolytica for the production of astaxanthin.The high astaxanthin content and titer obtained even in a small-scale cultivation demonstrates a strong potential for Y.lipolytica-based fermentation process for astaxanthin production. | Kanchana Rueksomtawin Kildegaard Belen Adiego-Perez David Domenech Belda Jaspreet Kaur Khangura Carina Holkenbrink Irina Borodina | 2017 | Synthetic and Systems Biotechnology2017，2，4： | 12 |
| 3 | Systems metabolic engineering strategies for the production of amino acids显示文摘Systems metabolic engineering is a multidisciplinary area that integrates systems biology,synthetic biology and evolutionary engineering.It is an efficient approach for strain improvement and process optimization,and has been successfully applied in the microbial production of various chemicals including amino acids.In this review,systems metabolic engineering strategies including pathwayfocused approaches,systems biology-based approaches,evolutionary approaches and their applications in two major amino acid producing microorganisms:Corynebacterium glutamicum and Escherichia coli,are summarized. | Qian Ma Quanwei Zhang Qingyang Xu Chenglin Zhang Yanjun Li Xiaoguang Fan Xixian Xie Ning Chen | 2017 | Synthetic and Systems Biotechnology2017，2，2： | 10 |
| 4 | Interrogation of Streptomyces avermitilis for efficient production of avermectins显示文摘The 2015 Nobel Prize in Physiology or Medicine has been awarded to avermectins and artemisinin,respectively.Avermectins produced by Streptomyces avermitilis are excellent anthelmintic and potential antibiotic agents.Because wild-type strains only produce low levels of avermectins,much research effort has focused on improvements in avermectin production to meet the ever increasing demand for such compounds.This review describes the strategies that have been widely employed and the future prospects of synthetic biology applications in avermectin yield improvement.With the help of genome sequencing of S.avermitilis and an understanding of the avermectin biosynthetic/regulatory pathways,synthetic and systems biotechnology approaches have been applied for precision engineering.We focus on the design and synthesis of biological chassis,parts,devices,and modules from diverse microbes to reconstruct and optimize their dynamic processes,as well as predict favorable effective overproduction of avermectins by a 4Ms strategy(Mine,Model,Manipulation,and Measurement). | Jinsong Chen Mei Liu Xueting Liu Jin Miao Chengzhang Fu Heyong Gao Rolf Muller Qing Zhang Lixin Zhang | 2016 | Synthetic and Systems Biotechnology2016，1，1： | 10 |
| 5 | The secondary metabolite bioinformatics portal: Computational tools to facilitate synthetic biology of secondary metabolite production显示文摘Natural products are among the most important sources of lead molecules for drug discovery.With the development of affordable whole-genome sequencing technologies and other‘omics tools,the field of natural products research is currently undergoing a shift in paradigms.While,for decades,mainly analytical and chemical methods gave access to this group of compounds,nowadays genomics-based methods offer complementary approaches to find,identify and characterize such molecules.This paradigm shift also resulted in a high demand for computational tools to assist researchers in their daily work.In this context,this review gives a summary of tools and databases that currently are available to mine,identify and characterize natural product biosynthesis pathways and their producers based on‘omics data.A web portal called Secondary Metabolite Bioinformatics Portal(SMBP at http://gffzz02e507a9d8884f08hk5p90npok0wu6wup.ffgz.tsg.suse.edu.cn)is introduced to provide a one-stop catalog and links to these bioinformatics resources.In addition,an outlook is presented how the existing tools and those to be developed will influence synthetic biology approaches in the natural products field. | Tilmann Weber Hyun Uk Kim | 2016 | Synthetic and Systems Biotechnology2016，1，2： | 10 |
| 6 | A LuxR family transcriptional regulator AniF promotes the production of anisomycin and its derivatives in Streptomyces hygrospinosus var.beijingensis显示文摘The protein synthesis inhibitor anisomycin features a unique benzylpyrrolidine system and exhibits potent selective activity against pathogenic protozoa and fungi.It is one of the important effective components in Agricultural Antibiotic120,which has been widely used as naturally-originated agents for treatment of crop decay in China.The chemical synthesis of anisomycin has recently been reported,but the complex process with low productivity made the biosynthesis still to be a vital mainstay in efforts.The biosynthetic gene cluster(BGC)of anisomycin in Streptomyces hygrospinosus var.beijingensis has been identified in our previous work,while poor understanding of the regulatory mechanism limited the yield enhancement via regulation engineering of S.hygrospinosus var.beijingensis.In this study here,we characterized AniF as an indispensable LuxR family transcriptional regulator for the activation of anisomycin biosynthesis.The genetic manipulations of aniF and the real-time quantitative PCR(RT-qPCR)revealed that it positively regulated the transcription of the anisomycin BGC.Moreover,the overexpression of aniF contributed to the improvement of the production of anisomycin and its derivatives.Dissection of the mechanism underlying the function of AniF revealed that it directly activated the transcription of the genes aniR-G involved in anisomycin biosynthesis.Especially,one AniF-binding site in the promoter region of aniR was identified by DNase I footprinting assay and an inverted repeat sequence(5′-GGGC-3′)composed of two 4-nt half sites in the protected region was found.Taken together,our systematic study confirmed the positive regulatory role of AniF and might facilitate the future construction of engineering strains with high productivity of anisomycin and its derivatives. | Jufang Shen Lingxin Kong Yan Li Xiaoqing Zheng Qing Wang Weinan Yang Zixin Deng Delin You | 2019 | Synthetic and Systems Biotechnology2019，4，1： | 10 |
| 7 | Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products显示文摘The methylotrophic yeast Pichia pastoris(a.k.a.Komagataella phaffii)is one of the most commonly used hosts for industrial production of recombinant proteins.As a non-conventional yeast,P.pastoris has unique biological characteristics and its expression system has been well developed.With the advances in synthetic biology,more efforts have been devoted to developing P.pastoris into a chassis for the production of various high-value compounds,such as natural products.This review begins with the introduction of synthetic biology tools for the engineering of P.pastoris,including vectors,promoters,and terminators for heterologous gene expression as well as Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated System(CRISPR/Cas)for genome editing.This review is then followed by examples of the production of value-added natural products in metabolically engineered P.pastoris strains.Finally,challenges and outlooks in developing P.pastoris as a synthetic biology chassis are prospected. | Jucan Gao Lihong Jiang Jiazhang Lian | 2021 | Synthetic and Systems Biotechnology2021，6，2： | 9 |
| 8 | A brief overview of synthetic biology research programs and roadmap studies in the United States显示文摘The United States is a leading nation in the development of synthetic biology,an emerging engineering discipline to create,control and reprogram biological systems.With strategic investment from its government agencies,the U.S.has established numerous research centers and programs in synthetic biology,enabling significant advances in foundational tool development and practical applications ranging from bioenergy,biomanufacturing,to biomedicine.To maintain its leadership in synthetic biology,U.S,has conducted several roadmap studies to provide strategic visions and action recommendations.Here we will provide a brief overview of the major research programs and roadmap studies of synthetic biology in the U.S. | Tong Si Huimin Zhao | 2016 | Synthetic and Systems Biotechnology2016，1，4： | 9 |
| 9 | Cell-free synthetic biology:Engineering in an open world显示文摘Cell-free synthetic biology emerges as a powerful and flexible enabling technology that can engineer biological parts and systems for life science applications without using living cells.It provides simpler and faster engineering solutions with an unprecedented freedom of design in an open environment than cell system.This review focuses on recent developments of cell-free synthetic biology on biological engineering fields at molecular and cellular levels,including protein engineering,metabolic engineering,and artificial cell engineering.In cell-free protein engineering,the direct control of reaction conditions in cell-free system allows for easy synthesis of complex proteins,toxic proteins,membrane proteins,and novel proteins with unnatural amino acids.Cell-free systems offer the ability to design metabolic pathways towards the production of desired products.Buildup of artificial cells based on cell-free systems will improve our understanding of life and use them for environmental and biomedical applications. | Yuan Lu | 2017 | Synthetic and Systems Biotechnology2017，2，1： | 9 |
| 10 | Production of anthocyanins in metabolically engineered microorganisms:Current status and perspectives显示文摘Microbial production of plant-derived natural products by engineered microorganisms has achieved great success thanks to large extend to metabolic engineering and synthetic biology.Anthocyanins,the water-soluble colored pigments found in terrestrial plants that are responsible for the red,blue and purple coloration of many flowers and fruits,are extensively used in food and cosmetics industry;however,their current supply heavily relies on complex extraction from plant-based materials.A promising alternative is their sustainable production in metabolically engineered microbes.Here,we review the recent progress on anthocyanin biosynthesis in engineered bacteria,with a special focus on the systematic engineering modifications such as selection and engineering of biosynthetic enzymes,engineering of transportation,regulation of UDP-glucose supply,as well as process optimization.These promising engineering strategies will facilitate successful microbial production of anthocyanins in industry in the near future. | Jian Zha Mattheos A.G.Koffas | 2017 | Synthetic and Systems Biotechnology2017，2，4： | 8 |
| 11 | Metabolic engineering for the microbial production of isoprenoids:Carotenoids and isoprenoid-based biofuels显示文摘Isoprenoids are the most abundant and highly diverse group of natural products.Many isoprenoids have been used for pharmaceuticals,nutraceuticals,flavors,cosmetics,food additives and biofuels.Carotenoids and isoprenoid-based biofuels are two classes of important isoprenoids.These isoprenoids have been produced microbially through metabolic engineering and synthetic biology efforts.Herein,we briefly review the engineered biosynthetic pathways in well-characterized microbial systems for the production of carotenoids and several isoprenoid-based biofuels. | Fu-Xing Niu Qian Lu Yi-Fan Bu Jian-Zhong Liu | 2017 | Synthetic and Systems Biotechnology2017，2，3： | 8 |
| 12 | Engineering bacteria for enhanced polyhydroxyalkanoates(PHA)biosynthesis显示文摘Polyhydroxyalkanoates(PHA)have been produced by some bacteria as bioplastics for many years.Yet their commercialization is still on the way.A few issues are related to the difficulty of PHA commercialization:namely,high cost and instabilities on molecular weights(Mw)and structures,thus instability on thermo-mechanical properties.The high cost is the result of complicated bioprocessing associated with sterilization,low conversion of carbon substrates to PHA products,and slow growth of microorganisms as well as difficulty of downstream separation.Future engineering on PHA producing microorganisms should be focused on contamination resistant bacteria especially extremophiles,developments of engineering approaches for the extremophiles,increase on carbon substrates to PHA conversion and controlling Mw of PHA.The concept proof studies could still be conducted on E.coli or Pseudomonas spp.that are easily used for molecular manipulations.In this review,we will use E.coli and halophiles as examples to show how to engineer bacteria for enhanced PHA biosynthesis and for increasing PHA competitiveness. | Guo-Qiang Chen Xiao-Ran Jiang | 2017 | Synthetic and Systems Biotechnology2017，2，3： | 8 |
| 13 | Inactivation of SACE_3446, a TetR family transcriptional regulator, stimulates erythromycin production in Saccharopolyspora erythraea显示文摘Erythromycin A is a widely used antibiotic produced by Saccharopolyspora erythraea;however,its biosynthetic cluster lacks a regulatory gene,limiting the yield enhancement via regulation engineering of S.erythraea.Herein,six TetR family transcriptional regulators(TFRs)belonging to three genomic context types were individually inactivated in S.erythraea A226,and one of them,SACE_3446,was proved to play a negative role in regulating erythromycin biosynthesis.EMSA and qRT-PCR analysis revealed that SACE_3446 covering intact N-terminal DNA binding domain specifically bound to the promoter regions of erythromycin biosynthetic gene eryAI,the resistant gene ermE and the adjacent gene SACE_3447(encoding a longchain fatty-acid CoA ligase),and repressed their transcription.Furthermore,we explored the interaction relationships of SACE_3446 and previously identified TFRs(SACE_3986 and SACE_7301)associated with erythromycin production.Given demonstrated relatively independent regulation mode of SACE_3446 and SACE_3986 in erythromycin biosynthesis,we individually and concomitantly inactivated them in an industrial S.erythraea WB.Compared with WB,the WBΔ3446 and WBΔ3446Δ3986 mutants respectively displayed 36%and 65%yield enhancement of erythromycin A,following significantly elevated transcription of eryAI and ermE.When cultured in a 5 L fermentor,erythromycin A ofWBΔ3446 and WBΔ3446Δ3986 successively reached 4095 mg/L and 4670 mg/L with 23%and 41%production improvement relative to WB.The strategy reported here will be useful to improve antibiotics production in other industrial actinomycete. | Hang Wu Yansheng Wang Li Yuan Yongrong Mao Weiwei Wang Lin Zhu Panpan Wu Chengzhang Fu Rolf Muller David T.Weaver Lixin Zhang Buchang Zhang | 2016 | Synthetic and Systems Biotechnology2016，1，1： | 7 |
| 14 | Systematic optimization of the yeast cell factory for sustainable and high efficiency production of bioactive ginsenoside compound K显示文摘Ginsenoside Compound K(CK)has been recognized as a major functional component that is absorbed into the systemic circulation after oral administration of ginseng.CK demonstrates diverse bioactivities.A phase I clinical study indicated that CK was a potential candidate for arthritis therapy.However,a phase II clinical study was suspended because of the high cost associated with the present CK manufacturing approach,which is based on the traditional planting-extracting-biotransforming process.We previously elucidated the complete CK biosynthetic pathway and realized for the first time de novo biosynthesis of CK from glucose by engineered yeast.However,CK production was not sufficient for industrial application.Here,we systematically engineered Saccharomyces cerevisiae to achieve high titer production of CK from glucose using a previously constructed protopanaxadiol(PPD)-producing chassis,optimizing UGTPg1 expression,improving UDP-glucose biosynthesis,and tuning down UDP-glucose consumption.Our final engineered yeast strain produced CK with a titer of 5.74 g/L in fed-batch fermentation,which represents the highest CK production in microbes reported to date.Once scaled-up,this high titer de novo microbial biosynthesis platform will enable a robust and stable supply of CK,thus facilitating study and medical application of CK. | Pingping Wang Jiali Wang Guoping Zhao Xing Yan Zhihua Zhou | 2021 | Synthetic and Systems Biotechnology2021，6，2： | 7 |
| 15 | Improvement of pristinamycin I(PI)production in Streptomyces pristinaespiralis by metabolic engineering approaches显示文摘Pristinamycin,produced by Streptomyces pristinaespiralis,which is a streptogramin-like antibiotic consisting of two chemically unrelated components:pristinamycin I(PI)and pristinamycin II(PII),shows potent activity against many antibiotic-resistant pathogens.However,so far pristinamycin production titers are still quite low,particularly those of PI.In this study,we constructed a PI single component producing strain by deleting the PII biosynthetic genes(snaE1 and snaE2).Then,two metabolic engineering approaches,including deletion of the repressor gene papR3 and chromosomal integration of an extra copy of the PI biosynthetic gene cluster(BGC),were employed to improve PI production.The final engineered strain DPIIDpapR3/PI produced a maximum PI level of 132 mg/L,with an approximately 2.4-fold higher than that of the parental strain S.pristinaespiralis HCCB10218.Considering that the PI biosynthetic genes are clustered in two main regions in the 210 kb“supercluster”containing the PI and PII biosynthetic genes as well as a cryptic polyketide BGC,these two regions were cloned separately and then were successfully assembled into the PI BGC by the transformation-associated recombination(TAR)system.Collectively,the metabolic engineering approaches employed is very efficient for strain improvement in order to enhance PI titer. | Jiali Meng Rongrong Feng Guosong Zheng Mei Ge Yvonne Mast Wolfgang Wohlleben Jufang Gao Weihong Jiang Yinhua Lu | 2017 | Synthetic and Systems Biotechnology2017，2，2： | 7 |
| 16 | Regulation of antibiotic biosynthesis in actinomycetes:Perspectives and challenges显示文摘Actinomycetes are the main sources of antibiotics.The onset and level of production of each antibiotic is subject to complex control by multi-level regulators.These regulators exert their functions at hierarchical levels.At the lower level,cluster-situated regulators(CSRs)directly control the transcription of neighboring genes within the gene cluster.Higher-level pleiotropic and global regulators exert their functions mainly through modulating the transcription of CSRs.Advances in understanding of the regulation of antibiotic biosynthesis in actinomycetes have inspired us to engineer these regulators for strain improvement and antibiotic discovery. | Junhong Wei Lang He Guoqing Niu | 2018 | Synthetic and Systems Biotechnology2018，3，4： | 6 |
| 17 | Improving acarbose production and eliminating the by-product component C with an efficient genetic manipulation system of Actinoplanes sp.SE50/110显示文摘Theα-glucosidase inhibitor acarbose is commercially produced by Actinoplanes sp.and used as a potent drug in the treatment of type-2 diabetes.In order to improve the yield of acarbose,an efficient genetic manipulation system for Actinoplanes sp.was established.The conjugation system between E.coli carryingØC31-derived integrative plasmids and the mycelia of Actinoplanes sp.SE50/110 was optimized by adjusting the parameters of incubation time of mixed culture(mycelia and E.coli),quantity of recipient cells,donor-to-recipient ratio and the concentration of MgCl2,which resulted in a high conjugation efficiency of 29.4%.Using this integrative system,a cloned acarbose biosynthetic gene cluster was introduced into SE50/110,resulting in a 35%increase of acarbose titer from 2.35 to 3.18 g/L.Alternatively,a pIJ101-derived replicating plasmid combined with the counter-selection system CodA(sm)was constructed for gene inactivation,which has a conjugation frequency as high as 0.52%.Meanwhile,almost all 5-flucytosine-resistant colonies were sensitive to apramycin,among which 75%harbored the successful deletion of targeted genes.Using this replicating vector,the maltooligosyltrehalose synthase gene treY responsible for the accumulation of component C was inactivated,and component C was eliminated as detected by LC-MS.Based on an efficient genetic manipulation system,improved acarbose production and the elimination of component C in our work paved a way for future rational engineering of the acarbose-producing strains. | Qinqin Zhao Huixin Xie Yao Peng Xinran Wang Linquan Bai | 2017 | Synthetic and Systems Biotechnology2017，2，4： | 6 |
| 18 | Transcription control engineering and applications in synthetic biology显示文摘In synthetic biology,researchers assemble biological components in new ways to produce systems with practical applications.One of these practical applications is control of the flow of genetic information(from nucleic acid to protein),a.k.a.gene regulation.Regulation is critical for optimizing protein(and therefore activity)levels and the subsequent levels of metabolites and other cellular properties.The central dogma of molecular biology posits that information flow commences with transcription,and accordingly,regulatory tools targeting transcription have received the most attention in synthetic biology.In this mini-review,we highlight many past successes and summarize the lessons learned in developing tools for controlling transcription.In particular,we focus on engineering studies where promoters and transcription terminators(cis-factors)were directly engineered and/or isolated from DNA libraries.We also review several well-characterized transcription regulators(trans-factors),giving examples of how cis-and trans-acting factors have been combined to create digital and analogue switches for regulating transcription in response to various signals.Last,we provide examples of how engineered transcription control systems have been used in metabolic engineering and more complicated genetic circuits.While most of our mini-review focuses on the well-characterized bacterium Escherichia coli,we also provide several examples of the use of transcription control engineering in non-model organisms.Similar approaches have been applied outside the bacterial kingdom indicating that the lessons learned from bacterial studies may be generalized for other organisms. | Michael D.Engstrom Brian F.Pfleger | 2017 | Synthetic and Systems Biotechnology2017，2，3： | 6 |
| 19 | Reactive oxygen species and antioxidant properties from mushrooms显示文摘Preventive medicine and food industry have shown an increased interest in the development of natural antioxidants,since those most commonly used synthetic antioxidants may have restricted use in food.This could explain why there is currently much research on the antioxidant properties from natural products such as mushrooms.Many mushrooms have been reported to possess antioxidant properties,which enable them to neutralize free radicals.The oxygen molecule is a free radical,which lead to the generation of the reactive oxygen species and can damage the cells.Cell damage caused by free radicals appears to be a major contributor to aging and degenerative diseases.Mushrooms antioxidant components are found in fruit bodies,mycelium and culture both,which include polysaccharides,tocopherols,phenolics,carotenoids,ergosterol and ascorbic acid among others.Fruit bodies or mycelium can be manipulated to produce active compounds in a relatively short period of time,which represent a significant advantage in antioxidant compounds extraction from mushrooms.Antioxidant compounds may be extracted to be used as functional additives or mushrooms can be incorporated into our food regime,representing an alternative source of food to prevent damage caused by oxidation in the human body. | Carmen Sanchez | 2017 | Synthetic and Systems Biotechnology2017，2，1： | 6 |
| 20 | Enhancement of precursor amino acid supplies for improving bacitracin production by activation of branched chain amino acid transporter BrnQ and deletion of its regulator gene lrp in Bacillus licheniformis显示文摘Bacitracin,a new type of cyclic peptide antibiotic,is widely used as the feed additive in feed industry.Branched chain amino acids(BCAAs)are the key precursors for bacitracin synthesis.In this research,soybean meal was served as the raw material to supply precursor amino acids for bacitracin synthesis,and enhanced production of bacitracin was attempted by engineering BCAA transporter BrnQ and its regulator Lrp in the bacitracin industrial production strain Bacillus licheniformis DW2.Firstly,our results confirmed that Lrp negatively affected bacitracin synthesis in DW2,and deletion of lrp improved intracellular BCAA accumulations,as well as the expression level of BCAA transporter BrnQ,which further led to a 14.71%increase of bacitracin yield,compared with that of DW2.On the contrary,overexpression of Lrp decreased bacitracin yield by 12.28%.Secondly,it was suggested that BrnQ acted as a BCAA importer in DW2,and overexpression of BrnQ enhanced the intracellular BCAA accumulations and 10.43%of bacitracin yield.While,the bacitracin yield decreased by 18.27%in the brnQ deletion strain DW2△brnQ.Finally,BrnQ was further overexpressed in lrp deletion strain DW2△lrp,and bacitracin yield produced by the final strain DW2△lrp::BrnQ was 965.34 U/mL,increased by 22.42%compared with that of DW2(788.48 U/mL).Collectively,this research confirmed that Lrp affected bacitracin synthesis via regulating the expression of BCAA transporter BrnQ and BCAA distributions,and provided a promising strain for industrial production of bacitracin. | Jiang Zhu Dongbo Cai Haixia Xu Ziwei Liu Bowen Zhang Fei Wu Junhui Li Shouwen Chen | 2018 | Synthetic and Systems Biotechnology2018，3，4： | 6 |
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