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| 1 | Direct reprogramming of Sertoli cells into multipotent neural stem cells by defined factors显示文摘Multipotent 神经干细胞为房间治疗保持大诺言。到导致的 pluripotent 干细胞以及成熟神经原的成纤维细胞的 reprogramming 建议可能性没有首先建立 pluripotency,把一个严重地区分的体的房间变换成一个 multipotent 状态。这里,我们证明房间从中层导出的那 Sertoli 能直接被变换成拥有神经干细胞性质的一个 multipotent 状态。导致的神经干细胞(iNSCs ) 表示多重 NSC 特定的标记,展出类似于正常 NSC 的全球基因表示侧面,并且能够自强并且区分功能的神经原进神经胶质并且 electrophysiologically。为酷氨酸 hydroxylase (TH ) 积极的导出 iNSC 的神经原污点, γ -aminobutyric 酸,和胆碱 acetyltransferase。另外, iNSCs 能熬过并且产生跟随移植进有牙齿的回转的触处。iNSCs 的产生可以为疾病建模和再生药有重要含意。 | Chao Sheng Qinyuan Zheng Jianyu Wu Zhen Xu Libin Wang Wei Li Haijiang Zhang Xiao-Yang Zhao Lei Liu Ziwei Wang Changlong Guo Hua-Jun Wu Zhonghua Liu Liu Wang Shigang He Xiu-Jie Wang Zhiguo Chen Qi Zhou | 2012 | Cell Research2012，22，1： | 31 |
| 2 | Neutrophil extracellular traps mediate the crosstalk between glioma progression and the tumor microenvironment via the HMGB1/RAGE/IL-8 axis显示文摘Objective:Neutrophil extracellular traps(NETs)produced by tumor-infiltrating neutrophils(TINs)are associated with poor prognosis in patients with several types of cancer.However,the mechanisms underlying the involvement of NETs in glioma progression remain largely unknown.This study aimed to elucidate the roles of NETs in biological processes that drive the crosstalk between glioma progression and the tumor microenvironment.Methods:Neutrophil infiltration and NETs formation were investigated in glioma tissue through immunohistochemistry,and their relationships with clinicopathological features and outcomes were statistically evaluated.The effects of NETs on glioma cell progression were studied in a co-culture system.In vivo and in vitro experiments validated the reactive oxygen species activity and cytokine production of TINs,as well as the ERK signaling pathway activation and the metastasis of gliomas.Results:Neutrophil infiltration and NETs formation were induced in high-grade glioma compared with low-grade glioma.NETs induced by TINs were determined to be an oncogenic marker of high-grade gliomas and to be involved in cell proliferation and invasion.NETs overproduction promoted glioma cell proliferation,migration,and invasion.Furthermore,HMGB1 was found to bind to RAGE and activate the NF-κB signaling pathway in vitro.In addition,NETs stimulated the NF-κB signaling pathway,thus promoting IL-8 secretion in glioblastoma.Subsequently,IL-8 recruited neutrophils which in turn mediated NETs formation via the PI3 K/AKT/ROS axis in TINs.Conclusions:Our results suggest that NETs produced by TINs mediate the crosstalk between glioma progression and the tumor microenvironment by regulating the HMGB1/RAGE/IL-8 axis.Targeting NETs formation or IL-8 secretion may be an effective approach to inhibit glioma progression. | Caijun Zha Xiangqi Meng Lulu Li Shan Mi Da Qian Ziwei Li Pengfei Wu Shaoshan Hu Shihong Zhao Jinquan Cai Yanhong Liu | 2020 | Cancer Biology & Medicine2020，17，1： | 19 |
| 3 | A data-driven health indicator extraction method for aircraft air conditioning system health monitoring显示文摘Prognostics and Health Management(PHM) has become a very important tool in modern commercial aircraft. Considering limited built-in sensing devices on the legacy aircraft model,one of the challenges for airborne system health monitoring is to find an appropriate health indicator that is highly related to the actual degradation state of the system. This paper proposed a novel health indicator extraction method based on the available sensor parameters for the health monitoring of Air Conditioning System(ACS) of a legacy commercial aircraft model. Firstly, a specific Airplane Condition Monitoring System(ACMS) report for ACS health monitoring is defined. Then a non-parametric modeling technique is adopted to calculate the health indicator based on the raw ACMS report data. The proposed method is validated on a single-aisle commercial aircraft widely used for short and medium-haul routes, using more than 6000 ACMS reports collected from a fleet of aircraft during one year. The case study result shows that the proposed health indicator can effectively characterize the degradation state of the ACS, which can provide valuable information for proactive maintenance plan in advance. | Jianzhong SUN Chaoyi LI Cui LIU Ziwei GONG Ronghui WANG | 2019 | Chinese Journal of Aeronautics2019，32，2： | 14 |
| 4 | Antimony smelting process generating solid wastes and dust:Characterization and leaching behaviors显示文摘A large amount of solid waste has been produced by the antimony smelting process in the'World Capital of Antimony', Xikuangshan area in China. This study comprehensively investigated the physical and chemical characteristics of the various solid wastes, as well as the leaching behavior of the solid wastes, which included water-quenched slag,arsenic-alkali residue, desulfurized slag and blast furnace dust. These four types of waste were enriched in a variety of heavy metals and metalloids and more specifically with As and Sb levels up to 8.6 × 104 and 3.16 × 105mg/kg, respectively, in arsenic-alkali residue. For desulfurized slag and water-quenched slag, the leaching concentration of Sb significantly exceeded the acceptable limits during the leaching tests using the toxicity characteristic leaching procedure and the synthetic precipitation leaching procedure. In addition, As leaching in arsenic-alkali residue was extraordinarily hazardous, being three orders of magnitude higher than the regulatory level of As. According to the results of the extraction tests, all the tested wastes were classified as hazardous waste. | Xuejun Guo Kunpeng Wang Mengchang He Ziwei Liu Hailin Yang Sisi Li | 2014 | Journal of Environmental Sciences2014，26，7： | 13 |
| 5 | Laser joining of CFRTP to titanium alloy via laser surface texturing显示文摘Grid pattern was textured on Ti-6 Al-4 V alloy(TC4)substrate surface by nanosecond laser system.Laser joining of carbon fiber reinforced thermoplastic composite(CFRTP)to TC4 joints were performed,and the effect of texture grid depth was investigated.The contact angle of molten CFRTP on textured TC4 surface was measured and the tensile-shear force was tested.The fracture surface and interface morphology were observed.The results indicated that the wettability of molten CFRTP on TC4 surface improved remarkably after laser textured TC4.Shear force of CFRTP/TC4 joints was increased by 156%after laser textured TC4 surface.When the depth of grid was deeper than 100μm,contact angle increased and incomplete filling of molten CFRTP in grid occurred,the shear force thus decreased gradually.Resin-carbon fibers mixture was adhered on the fracture surface of TC4,and the variation tendency of adhesion ratio was consistent with that of shear force.TC4 matrix was exfoliated from substrate and adhered at the fracture surface of CFRTP,indicating stronger mechanical interlocking occurred at the joining interface after laser textured TC4 surface.Beside mechanical interlocking,compound layer consisted of CTi_(0.42)V_(1.58)carburization phase was also confirmed at interface,suggesting that chemical bonding also occurred at the joining interface. | Caiwang TAN Jianhui SU Ziwei FENG Yifan LIU Bo CHEN Xiaoguo SONG | 2021 | Chinese Journal of Aeronautics2021，34，5： | 11 |
| 6 | Internet of Things to network smart devices for ecosystem monitoring显示文摘Smart, real-time, low-cost, and distributed ecosystem monitoring is essential for understanding and managing rapidly changing ecosystems. However, new techniques in the big data era have rarely been introduced into operational ecosystem monitoring, particularly for fragile ecosystems in remote areas.We introduce the Internet of Things(IoT) techniques to establish a prototype ecosystem monitoring system by developing innovative smart devices and using IoT technologies for ecosystem monitoring in isolated environments. The developed smart devices include four categories: large-scale and nonintrusive instruments to measure evapotranspiration and soil moisture, in situ observing systems for CO2 and d13 C associated with soil respiration, portable and distributed devices for monitoring vegetation variables, and Bi-CMOS cameras and pressure trigger sensors for terrestrial vertebrate monitoring. These new devices outperform conventional devices and are connected to each other via wireless communication networks. The breakthroughs in the ecosystem monitoring IoT include new data loggers and longdistance wireless sensor network technology that supports the rapid transmission of data from devices to wireless networks. The applicability of this ecosystem monitoring IoT is verified in three fragile ecosystems, including a karst rocky desertification area, the National Park for Amur Tigers, and the oasis-desert ecotone in China. By integrating these devices and technologies with an ecosystem monitoring information system, a seamless data acquisition, transmission, processing, and application IoT is created. The establishment of this ecosystem monitoring IoT will serve as a new paradigm for ecosystem monitoring and therefore provide a platform for ecosystem management and decision making in the era of big data. | Xin Li Ning Zhao Rui Jin Shaomin Liu Xiaomin Sun Xuefa Wen Dongxiu Wu Yan Zhou Jianwen Guo Shiping Chen Ziwei Xu Mingguo Ma Tianming Wang Yonghua Qu Xinwei Wang Fangming Wu Yuke Zhou | 2019 | Science Bulletin2019，64，17： | 10 |
| 7 | A dual-pass data assimilation scheme for estimating surface fluxes with FY3A-VIRR land surface temperature显示文摘In this work, a dual-pass data assimilation scheme is developed to improve predictions of surface flux. Pass 1 of the dual-pass data assimilation scheme optimizes the model vegetation parameters at the weekly temporal scale, and Pass 2 optimizes the soil moisture at the daily temporal scale. Based on ensemble Kalman filter(EnKF), the land surface temperature(LST) data derived from the new generation of Chinese meteorology satellite(FY3A-VIRR) are assimilated into common land model(CoLM) for the first time. Six sites, Daman, Guantao, Arou, BJ, Miyun and Jiyuan, are selected for the data assimilation experiments and include different climatological conditions. The results are compared with those from a dataset generated by a multi-scale surface flux observation system that includes an automatic weather station(AWS), eddy covariance(EC) and large aperture scintillometer(LAS). The results indicate that the dual-pass data assimilation scheme is able to reduce model uncertainties and improve predictions of surface flux with the assimilation of FY3A-VIRR LST data. | XU TongRen LIU ShaoMin XU ZiWei LIANG ShunLin XU Lu | 2015 | Science China Earth Sciences2015，58，2： | 9 |
| 8 | Large-Scale Membrane Bioreactors for Industrial Wastewater Treatment in China:Technical and Economic Features,Driving Forces,and Perspectives显示文摘Membrane bioreactors(MBRs)have been and will continue playing an important role in industrial wastewater treatment and reuse in China.The sustainable development of MBR technology in its mature-application stage requires reciprocal interactions between engineering and research participants.Thus,in this study,a total of 182 large-scale MBR projects treating industrial wastewater(with individual treatment capacities5000 m3d1)commissioned and under construction from 2003 to 2019 were analyzed comprehensively.Fast growth of the cumulative treatment capacity was observed,with extension to diverse industries,and the super large-scale was enhanced recently.The treatment processes,pollutant removal efficiencies,and actual operational parameters were summarized regarding the particularity of industrial wastewater compared to municipal wastewater.Economic features including the total investment costs of the projects,their total footprint,and their operational energy consumption were analyzed as well.A vigorous MBR market has formed in China with the fast development of membrane elements and engineering suppliers,continuously increasing official oriented projects,and responsive and innovative business modes.MBR technology has been mostly applied in specific economic zones and water-deficient areas,but its widespread use all over China is foreseeable considering the vast future market for industrial wastewater treatment and recycling.The policy–economy and market–technology driving forces revealed that MBR is consistent with the national development demand.According to the survey and analysis,prospective development in both engineering and research aspects of MBR is proposed to maintain its competitive edge. | Jiao Zhang Kang Xiao Ziwei Liu Tingwei Gao Shuai Liang Xia Huang | 2021 | Engineering2021，7，6： | 9 |
| 9 | Distribution and characteristic of PAHs in snow of Fildes Peninsula显示文摘Polycyclic aromatic hydrocarbons (PAHs) investigation in different matrices has been reported largely,whereas reports on snow samples were limited.Snow,as the main matrix in the polar region,has an important study meaning.PAHs in snow samples were analyzed to investigate the distribution and contamination status of them in the Antarctic,as well as to provide some references for global migration of PAHs.Snow samples collected in Fildes Peninsula were enriched and separated by solid-phase membrane disks and eluted by methylene dichloride,then quantified by gas chromatography/mass spectrometry.All types of PAHs were detected except for Benzo(a)pyrene.Principal component analysis method was applied to characterize them.Three factors (Naphthalene,Fluorene and Phenanthrene) accounted for 60.57%,21.61% and 9.80%,respectively.The results showed that the major PAHs sources maybe the atmospheric transportation,and the combustion of fuel in Fildes Peninsula.The comparison of concentration and types of PAHs between accumulated snow and fresh snow showed that the main compound concentrations in accumulated snow samples were higher than those in fresh ones.The risk assessment indicated that the amount of PAHs in the snow samples would not lead to ecological risk. | Guangshui Na Chunyang Liu Zhen Wang Linke Ge Xindong Ma Ziwei Yao | 2011 | Journal of Environmental Sciences2011，23，9： | 8 |
| 10 | A review:Photonics devices,architectures,and algorithms for optical neural computing显示文摘The explosive growth of data and information has motivated various emerging non-von Neumann computational approaches in the More-than-Moore era.Photonics neuromorphic computing has attracted lots of attention due to the fascinating advantages such as high speed,wide bandwidth,and massive parallelism.Here,we offer a review on the optical neural computing in our research groups at the device and system levels.The photonics neuron and photonics synapse plasticity are presented.In addition,we introduce several optical neural computing architectures and algorithms including photonic spiking neural network,photonic convolutional neural network,photonic matrix computation,photonic reservoir computing,and photonic reinforcement learning.Finally,we summarize the major challenges faced by photonic neuromorphic computing,and propose promising solutions and perspectives. | Shuiying Xiang Yanan Han Ziwei Song Xingxing Guo Yahui Zhang Zhenxing Ren Suhong Wang Yuanting Ma Weiwen Zou Bowen Ma Shaofu Xu Jianji Dong Hailong Zhou Quansheng Ren Tao Deng Yan Liu Genquan Han Yue Hao | 2021 | Journal of Semiconductors2021，42，2： | 7 |
| 11 | Recent progress of integrated circuits and optoelectronic chips显示文摘Integrated circuits(ICs)and optoelectronic chips are the foundation stones of the modern information society.The IC industry has been driven by the so-called'Moore's law'in the past 60 years,and now has entered the post Moore's law era.In this paper,we review the recent progress of ICs and optoelectronic chips.The research status,technical challenges and development trend of devices,chips and integrated technologies of typical IC and optoelectronic chips are focused on.The main contents include the development law of IC and optoelectronic chip technology,the IC design and processing technology,emerging memory and chip architecture,brain-like chip structure and its mechanism,heterogeneous integration,quantum chip technology,silicon photonics chip technology,integrated microwave photonic chip,and optoelectronic hybrid integrated chip. | Yue HAO Shuiying XIANG Genquan HAN Jincheng ZHANG Xiaohua MA Zhangming ZHU Xingxing GUO Yahui ZHANG Yanan HAN Ziwei SONG Yan LIU Ling YANG Hong ZHOU Jiangyi SHI Wei ZHANG Min XU Weisheng ZHAO Biao PAN Yangqi HUANG Qi LIU Yimao CAI Jian ZHU Xin OU Tiangui YOU Huaqiang WU Bin GAO Zhiyong ZHANG Guoping GUO Yonghua CHEN Yong LIU Xiangfei CHEN Chunlai XUE Xingjun WANG Lixia ZHAO Xihua ZOU Lianshan YAN Ming LI | 2021 | Science China(Information Sciences)2021，64，10： | 7 |
| 12 | Large-scale blow spinning of heat-resistant nanofibrous air filters显示文摘Particulate matter(PM)pollution has become a serious problem worldwide and various kinds of nanofibrous filters aiming to solve the problem have been developed.It is urgent to remove PM from high-temperature pollution sources,such as industrial emissions,coal furnaces,and automobile exhaust gases.However,filtration at pollution sources remains challenging because most existing air filters are not resistant to high temperature.Herein,heat-resistant polyimide(PI)nanofibrous air filters are fabricated via a simple and scalable solution blow-spinning method.These air filters show excellent thermal stability at high temperature up to 420℃.They exhibit a filtration efficiency as high as 99.73%at ambient temperature and over 97%at 300℃.In addition,a field test shows that the filters remove>97%of PM from the car exhaust fumes.Hence,the blow-spun PI nanofibrous membranes combined with the facile preparation strategy have great potential in high temperature air filtration fields and other similar applications such as water purification and protein separation. | Ziwei Li Jianan Song Yuanzheng Long Chao Jia Zhenglian Liu Lei Li Cheng Yang Junchen Liu Sen Lin Haiyang Wang Yibo Liu Minghao Fang Hui Wu | 2020 | Nano Research2020，13，3： | 6 |
| 13 | Enhancement of precursor amino acid supplies for improving bacitracin production by activation of branched chain amino acid transporter BrnQ and deletion of its regulator gene lrp in Bacillus licheniformis显示文摘Bacitracin,a new type of cyclic peptide antibiotic,is widely used as the feed additive in feed industry.Branched chain amino acids(BCAAs)are the key precursors for bacitracin synthesis.In this research,soybean meal was served as the raw material to supply precursor amino acids for bacitracin synthesis,and enhanced production of bacitracin was attempted by engineering BCAA transporter BrnQ and its regulator Lrp in the bacitracin industrial production strain Bacillus licheniformis DW2.Firstly,our results confirmed that Lrp negatively affected bacitracin synthesis in DW2,and deletion of lrp improved intracellular BCAA accumulations,as well as the expression level of BCAA transporter BrnQ,which further led to a 14.71%increase of bacitracin yield,compared with that of DW2.On the contrary,overexpression of Lrp decreased bacitracin yield by 12.28%.Secondly,it was suggested that BrnQ acted as a BCAA importer in DW2,and overexpression of BrnQ enhanced the intracellular BCAA accumulations and 10.43%of bacitracin yield.While,the bacitracin yield decreased by 18.27%in the brnQ deletion strain DW2△brnQ.Finally,BrnQ was further overexpressed in lrp deletion strain DW2△lrp,and bacitracin yield produced by the final strain DW2△lrp::BrnQ was 965.34 U/mL,increased by 22.42%compared with that of DW2(788.48 U/mL).Collectively,this research confirmed that Lrp affected bacitracin synthesis via regulating the expression of BCAA transporter BrnQ and BCAA distributions,and provided a promising strain for industrial production of bacitracin. | Jiang Zhu Dongbo Cai Haixia Xu Ziwei Liu Bowen Zhang Fei Wu Junhui Li Shouwen Chen | 2018 | Synthetic and Systems Biotechnology2018，3，4： | 6 |
| 14 | Studying the urban hierarchical pattern and spatial structure of China using a synthesized gravity model显示文摘The Gravity Model(GM), one of the most classic models adopted by the domain of social sciences from physics, has been widely used to study the interactions between social identities. Previous research that has used the Gravity Model to study the interactions among cities have mostly used a single variable, such as population or GDP, to represent a city, which does not provide a comprehensive depiction of a city's influence. This paper develops a Synthesized Gravity Model(SGM) based on the traditional Gravity Model to study the evolution of the hierarchy of the Chinese urban system since the mid-1990 s. Under this model, socioeconomic variables are synthesized and are represented by the Influential Factor, while the Function Distance is derived from a Network Analysis that is based on multiple transportation methods. As an improvement on the GM, the SGM is used to accurately establish and represent the nodal structure of China's urban system, the evolution of its hierarchical structure,and the relationships that exist between the nodal structure and socioeconomic factors. The results based on the SGM indicate that China's national urban system is characterized by the emergence of urban clusters with stronger inter-city interactions since the 1990 s. However, the development among cities within certain urban clusters is not even, although the general pattern indicates a lessening inequality amongst cities. Spatially, while most cities at the top of the hierarchy are located in Eastern China, cities in the center and the west of the country are also gaining higher positions in the hierarchy over time. This paper is dedicated to improving the traditional GM in the applications of urban studies, while the system of Chinese cities is used to validate the SGM. | Ruibo HAN Huhua CAO Ziwei LIU | 2018 | Science China Earth Sciences2018，61，12： | 5 |
| 15 | Ultrasonication-assisted and gram-scale synthesis of Co-LDH nanosheet aggregates for oxygen evolution reaction显示文摘Electrochemical water splitting(EWS)is a highly clean and efficient method for high-purity hydrogen production.Unfortunately,EWS suffers from the sluggish and complex oxygen evolution reaction(OER)kinetics at anode.At present,the efficient,stable,and low-cost non-precious metal based OER electrocatalyst is still a great and long-term challenge for the future industrial application of EWS technology.Herein,we develop a simple and fast approach for gram-scale synthesis of flower-like cobalt-based layered double hydroxides nanosheet aggregates by ultrasonic synthesis,which show outstanding electrocatalytic performance for the oxygen evolution reaction in alkaline media,such as preeminent stability,small overpotential of 300 mV at 10 mA·cm^−2 and small Tafel slope of 110 mV·dec^−1. | Tian-Jiao Wang Xiaoyang Liu Ying Li Fumin Li Ziwei Deng Yu Chen | 2020 | Nano Research2020，13，1： | 5 |
| 16 | Automatic Modulation Recognition Based on CNN and GRU显示文摘Based on a comparative analysis of the Long Short-Term Memory(LSTM)and Gated Recurrent Unit(GRU)networks,we optimize the structure of the GRU network and propose a new modulation recognition method based on feature extraction and a deep learning algorithm.High-order cumulant,Signal-to-Noise Ratio(SNR),instantaneous feature,and the cyclic spectrum of signals are extracted firstly,and then input into the Convolutional Neural Network(CNN)and the parallel network of GRU for recognition.Eight modulation modes of communication signals are recognized automatically.Simulation results show that the proposed method can achieve high recognition rate at low SNR. | Fugang Liu Ziwei Zhang Ruolin Zhou | 2022 | Tsinghua Science and Technology2022，27，2： | 5 |
| 17 | Density functional theory(DFT) studies of vanadium-titanium based selective catalytic reduction(SCR) catalysts显示文摘Based on density functional theory(DFT)and basic structure models,the chemical reactions on the surface of vanadium-titanium based selective catalytic reduction(SCR)denitrification catalysts were summarized.Reasonable structural models(non-periodic and periodic structural models)are the basis of density functional calculations.A periodic structure model was more appropriate to represent the catalyst surface,and its theoretical calculation results were more comparable with the experimental results than a nonperiodic model.It is generally believed that the SCR mechanism where NH3 and NO react to produce N2 and H2 O follows an Eley-Rideal type mechanism.NH2 NO was found to be an important intermediate in the SCR reaction,with multiple production routes.Simultaneously,the effects of H2 O,SO2 and metal on SCR catalysts were also summarized. | Ziwei Zhao Erwei Li Yu Qin Xiaolong Liu Yang Zou Heng Wu Tingyu Zhu | 2020 | Journal of Environmental Sciences2020，32，4： | 4 |
| 18 | Post-marketing safety surveillance and reevaluation of Motherwort injection:A clinical study of 10094 cases显示文摘OBJECTIVE:To investigate the safety profiles of Motherwort injection(MI).METHODS:A multi-center,prospective and drugderived hospital intensive monitoring method was conducted to assess the safety of MI in real world applications.This study was based on a very large population after the injection was approved and marketed in China.All patients using the injection in participating hospitals were monitored to determine the incidence,pattern,severity and outcome of associated adverse events.RESULTS:The post-marketing surveillance was performed in 10 094 female patients from April to December,2015.The incidence of adverse drug reactions(ADRs) was 0.79‰(8/10 094).Among the 8 patients,the reported adverse events mainly included systemic abnormalities,such as fever,chills and eyelid edema;skin and appendages disorders,such as pruritus and rash;gastrointestinal disorders,such as nausea,abdominal distension and pain;heart rate and rhythm disorders,such as palpitation and increased heart rate.All of these ADRs were mild in severity.CONCLUSION:In this study the ADRs incidence rate of MI is very low,which supports that it is generally safe for use in obstetric and gynecological diseases.However,the total number of 8 ADRs recorded over a relatively short time span seems limited,and the low number of reports could not represent an absolute guarantee of safety. | Cao Shan Zhang Wenhao Zhao Ziwei Heng Mingli Bu Huaien Wang Hongwu Liu Xinghui Wang Zhong Cai Yan Ma Yuyan Cui Shihong Deng Jihong Ding Guifeng Ding Yajuan Dong Linhong Duan Zhentao Fan Ling Fan Yang Fu Fen He Jing Ji Shuying Jin Lin Li Hong Li Hongying Liao Tao Lu Wei Luo Xiucui Lü Zhihui Ma Fengchun Ma Dafeng Shi Tianyun Sun Juying Sun Xiaotong Teng Hong Wang Jinhua Wang Ruihua Wang Ying Wang Zhengling Xi Jie Xu Minjuan Xu Zhihong Yan Qian Yang Cuirong Yang Yimei Yin Jie Yu Jinhua Yuan Wenjun Zhang Guanli Zhang Meihua Zhao Renfeng Zhong Yonghong Zhou Jian | 2018 | Journal of Traditional Chinese Medicine2018，38，4： | 4 |
| 19 | A fully automated centrifugal microfluidic system for sample-to-answer viral nucleic acid testing显示文摘The outbreak of virus-induced infectious diseases poses a global public-health challenge.Nucleic acid amplification testing(NAAT)enables early detection of pandemic viruses and plays a vital role in preventing onward transmission.However,the requirement of skilled operators,expensive instrumentation,and biosafety laboratories has hindered the use of NAAT for screening and diagnosis of suspected patients.Here we report development of a fully automated centrifugal microfluidic system with sample-in-answer-out capability for sensitive,specific,and rapid viral nucleic acid testing.The release of nucleic acids and the subsequent reverse transcription loop-mediated isothermal amplification(RT-LAMP)were integrated into the reaction units of a microfluidic disc.The whole processing steps such as injection of reagents,fluid actuation by rotation,heating and temperature control,and detection of fluorescence signals were carried out automatically by a customized instrument.We validate the centrifugal microfluidic system using oropharyngeal swab samples spiked with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)armored RNA particles.The estimated limit of detection for armored RNA particles is 2 copies per reaction,the throughput is 21 reactions per disc,and the assay sample-to-answer time is approximately 70 min.This enclosed and automated microfluidic system efficiently avoids viral contamination of aerosol,and can be readily adapted for virus detection outside the diagnostic laboratory. | Fei Tian Chao Liu Jinqi Deng Ziwei Han Lu Zhang Qinghua Chen Jiashu Sun | 2020 | Science China Chemistry2020，63，10： | 4 |
| 20 | SOX4 contributes to TGF-β-induced epithelialemesenchymal transition and stem cell characteristics of gastric cancer cells显示文摘SOX4 is highly expressed in gastric cancer(GC)and is associated with tumor grade,metastasis and prognosis,however the mechanism is not clear.We report herein that SOX4 was upregulated and overexpression of SOX4 was associated with increased expression of the markers of Epithelialemesenchymal transition(EMT)and stemness in clinic patient samples.In vitro,overexpression of SOX4 promoted the invasion as showed by Transwell assay and stemness of GC cells as assessed by sphere formation assay,which was suppressed by silencing SOX4 with shRNA.Further studies showed that SOX4 up-regulated the expression of EMT transcription factors Twist1,snail1 and zeb1 and stemness transcription factors SOX2 and OCT4,and promoted the nuclear translocation of β-catenin.Moreover,we revealed that TGF-β treatment significantly up-regulated the expression of SOX4 and silencing SOX4 reversed TGF-β induced invasion and sphere formation ability of GC cells.Finally,we showed that SOX4 promoted the lung metastasis and tumor formation ability of gastric cancer cells in nude mice.Our results suggest that SOX4 is a target TGF-β signaling and mediates TGF-β-induced EMT and stem cell characteristics of GC cells,revealing a novel role of TGF-β/SOX4 axis in the regulation of malignant behavior of GC. | Xudong Peng Guangyi Liu Hongxia Peng Anqi Chen Lang Zha Ziwei Wang | 2018 | Genes & Diseases2018，5，1： | 4 |
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