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| 1 | Circulating tumor and cancer stem cells in hepatitis C virusassociated liver disease显示文摘AIM:To assess the role of circulating tumor cells(CTCs)and cancer stem cells(CSCs)in hepatitis C virus(HCV)-associated liver disease.METHODS:Blood and/or tissue samples were obtained from HCV(genotype 4)-associated hepatocellularcarcinoma patients(HCC;n=120),chronic hepatitis C patients(CH;n=30)and 33 normal control subjects(n=33).Serum levels of alpha-fetoprotein(AFP),alkaline phosphatase,and alanine and aspartate aminotransferases were measured.Cytokeratin 19(CK19)monoclonal antibody was used to enumerate CTCs,and CD133 and CD90 were used to enumerate CSCs by flow cytometry.The expression levels of the CSCs markers(CD133 and CD90)as well as telomerase,melanoma antigen encoding gene 1(MAGE1)and MAGE3 were assessed by RTPCR and quantitative real-time polymerase chain reactions.The number of CTCs and/or the expression levels of CK19,CD133,telomerase,MAGE1 and MAGE3 were correlated to the standard clinicopathologic prognostic factors and disease progression.RESULTS:Levels of AFP,alkaline phosphatase and aspartate aminotransferase were significantly different among the HCC,CH and control groups(P<0.001),whereas alanine aminotransferase differed significantly between patient(HCC and CH)and control groups(P<0.001).At the specified cutoff values determine by flow cytometry,CK19(49.8),CD90(400)and CD133(73)were significantly higher in the blood of HCC patients compared to those in the CH and control groups(P<0.001).On the other hand,CD133 at a 69.5 cutoff was significantly higher in the CH compared to the control group(P≤0.001).Telomerase,MAGE1 and MAGE3RNA were expressed in 55.71%,60.00%and 62.86%of the HCC patients,respectively,but were not detected in patients in the CH or control groups,which were statistically significant(Ps<0.001).The expression levels of telomerase,CD90,MAGE3,CD133 and CK19 were all significantly associated with high tumor grade and advanced stage in HCC patients(all P s<0.05).CONCLUSION:CTC counts and AFP,CK19,telomerase,and MAGE1/MAGE3 expression predict disease progression in patients with HCV,whereas telomerase,MAGE3,CD90,CD133 and CK19 are prognostic mark- | Abeer A Bahnassy Abdel-Rahman N Zekri Ahmed El-Bastawisy Amal Fawzy Marwa Shetta Nehal Hussein Dalia Omran Abdallah A S Ahmed Samir S El-Labbody | 2014 | World Journal of Gastroenterology2014，20，48： | 9 |
| 2 | Progression of diethylnitrosamine-induced hepatic carcinogenesis in carnitine-depleted rats显示文摘AIM:To investigate whether carnitine deficiency is a risk factor during the development of diethylnitrosamine (DENA)-induced hepatic carcinogenesis. METHODS:A total of 60 male Wistar albino rats were divided into six groups with 10 animals in each group.Rats in group 1(control group)received a single intraperitoneal(i.p.)injection of normal saline. Animals in group 2(carnitine-supplemented group) were given L-carnitine(200 mg/kg per day)in drinking water for 8 wk.Animals in group 3(carnitine-depleted group)were given D-carnitine(200 mg/kg per day)and mildronate(200 mg/kg per day)in drinking water for 8 wk.Rats in group 4(DENA group)were injected with a single dose of DENA(200 mg/kg,i.p.)and 2 wk later received a single dose of carbon tetrachloride(2 mL/kg) by gavage as 1:1 dilution in corn oil.Animals in group 5(DENA-carnitine depleted group)received the same treatment as group 3 and group 4.Rats in group 6 (DENA-carnitine supplemented group)received the same treatment as group 2 and group 4.RESULTS:Administration of DENA resulted in a significant increase in alanine transaminase(ALT), gamma-glutamyl transferase(G-GT),alkaline phosphatase(ALP),total bilirubin,thiobarbituric acid reactive substances(TBARS)and total nitrate/ nitrite(NOx)and a significant decrease in reduced glutathione(GSH),glutathione peroxidase(GSHPx), catalase(CAT)and total carnitine content in liver tissues.In the carnitine-depleted rat model,DENA induced a dramatic increase in serum ALT,G-GT,ALP and total bilirubin,as well as a progressive reduction in total carnitine content in liver tissues.Interestingly, L-carnitine supplementation resulted in a complete reversal of the increase in liver enzymes,TBARS and NOx,and a decrease in total carnitine,GSH,GSHPx, and CAT induced by DENA,compared with the control values.Histopathological examination of liver tissues confirmed the biochemical data,where L-carnitine prevented DENA-induced hepatic carcinogenesis while D-carnitine-mildronate aggravated DENA-induced hepatic damage. CONCLUSION:Data from this study suggest for the first time that:(1)carnitine deficiency is a risk factor and should be viewed as a mechanism in DENA- induced hepatic carcinogenesis;(2)oxidative stress plays an important role but is not the only cause of DENA-induced hepatic carcinogenesis;and(3) long-term L-carnitine supplementation prevents the development of DENA-induced liver cancer. | Salim S Al-Rejaie Abdulaziz M Aleisa Abdulaziz A Al-Yahya Saleh A Bakheet Abdulmalik Alsheikh Amal G Fatani Othman A Al-Shabanah Mohamed M Sayed-Ahmed | 2009 | World Journal of Gastroenterology2009，15，11： | 4 |
| 3 | Cytokine profile in Egyptian hepatitis C virus genotype-4 in relation to liver disease progression显示文摘AIM: To observe the imbalance between T helper cell Th1 and Th2 cytokines in several chronic hepatitis disease at different stages of disease progression.METHODS: We measured the cytokine levels of Th1 (IL-2 and IL-2R), Th2 (IL-10) and the pro-inflammatory cytokines (IL-6 and IL-6R and TNF and TNF-RⅠ and Ⅱ)by the ELISA technique in the sera of 33 hepatocellular carcinoma (HCC) patients and 20 chronic liver disease (CLD) patients. In addition, 20 asymptomatic hepatitis C virus carriers and 20 healthy subjects negative for hepatitis C virus(HCV) markers served as controls.RESULTS: Anti-HCV antibodies were found to be positive in 94% of HCC cases and 75% of CLD cases.On the other hand, HCV viremia was detected using RTPCR in 67% of HCC cases and 65% of CLD cases. HBsAg was positive in 9% of HCC cases and 30% of CLD cases.Also bilharzial-Ab was positive in 55% of HCC cases,65% of CLD cases and in 70% of asymptomatic carriers (ASC). HCC patients had significantly higher values of IL-2R, TNF-RⅡ (P<0.001), and TNF-RⅠ (P>0.05), but lower TNFα (P<0.001) and IL-6 (P = 0.032) in comparison to ASC. But, in comparison to non-cancer controls, HCC patients had higher values of IL-2R, IL-6R, TNF-RⅠ and TNF-RⅡ, but lower TNF-α (P<0.001). CLD patients had higher IL-2R, TNF-RⅠ, and TNF-RⅡ (P<0.001) than ASC. But, in comparison to non-cancer controls, CLD patients had higher values of IL-2R, TNF-RⅠ and TNF-RⅡ, but lower TNF-α (P<0.001). IL-10 was higher (though not significantly) in HCC and CLD patients than in symptomatic carriers and non-cancer controls.CONCLUSION: Liver disease progression from CLD to HCC due to HCV genotype-4 infection is associated with an imbalance between Th1 and Th2 cytokines. IL-2R,TNF-RⅠ, and TNF-RⅡ could be used as potential markers. | Abdel-Rahman N Zekri Mohammed S El-Din Ashour Ahmed Hassan Hanaa M Alam El-Din Amal MR El-Shehaby Maha A Abu-Shady | 2005 | World Journal of Gastroenterology2005，11，42： | 4 |
| 4 | Protection by L-carnitine against the inhibition of gene expression of heart fatty acid-binding protein by chronic administration of doxorubicin显示文摘 | Mohamad M Amal MR Somaya S | 2000 | J Egyptian Nat2000，12，4： | 1 |
| 5 | A study on the removal of humic acid using advanced oxidation processes显示文摘 | Lim S M Chiang K Amal R | 2007 | Separation Science and Technology2007，42，7： | 1 |
| 6 | Formation of fluid heavy oil-in-water emulsions for pipeline transportationt显示文摘 | Nehal S A Amal M N Nael N Z | 1999 | Fuel1999，78，59： | 1 |
| 7 | Direct repair ( nerve gra- fting), neurotization, and end-to-side neurorrhaphy in the treatment of brachial plexus injury 显示文摘 | Haninec P S(amal F Tom(as R | 2007 | J Neurosurg2007，106，： | 1 |
| 8 | Sustained increase in aortic endothelial nitric oxide synthase expression in vivo in a model of chronic high blood flow显示文摘 | Nadaud S Philippe M Amal JF | 1996 | Circ Res1996，79，： | 1 |
| 9 | Synthesis of a novelmagnetic photocatalyst by direct deposition of nano sized TiO2 crystals onto a magnetic core 显示文摘 | WATSON S BEYDOUN D AMAL R | 2002 | J Photochem Photobio A:Chem2002，148，： | 1 |
| 10 | Prenatal hyperandrogeni- zation induces metabolic and endocrine alterations which depend on the levels of testosterone exposure 显示文摘 | Amalli S Velez LM Heber MF | 2012 | LoS One2012，7，37： | 1 |
| 11 | Synthesis of a novel magnetic photocatalyst by direct deposition of nanosized crystals onto a magnetic core 显示文摘 | Watson S Beydoun D Amal R | 2002 | Journal of Photochemistry and Photobiology A2002，148，1： | 1 |
| 12 | Synthesis of a novel magnetic photocatalyst by direct deposition of nanosized TiO2 crystals onto a magnetic core显示文摘 | Watson S Beydoun D Amal R | 2002 | Journal of Photochemistry and Photobiology A: Chemistry2002，148，13： | 1 |
| 13 | Copper MOF: scope and limitation in catalytic hydroxylation and nitration of aryl halides显示文摘 | Priyadarshini S Amal Joseph J Lakshmi Kantam M | 2013 | Tetrahedron2013，31，： | 1 |
| 14 | Synthesis of a novel magnetic photocatalyst by direct deposition of nanosized TiO2 crystals onto a magnetic core 显示文摘 | Watson S Beydoun D Amal R | 2002 | Journal of photochemistry and photobiology A : Chemistry2002，148，13： | 1 |
| 15 | Synthesis of a novel magnetic photocatalyst by direct deposition of nanosized TiO2 crystals onto a magnetic core显示文摘 | Watson S Beydoun D Amal R | 2002 | Photochem Photobio A:Chem2002，148，： | 1 |
| 16 | Sulfonic acid resin and copper salts: a novel heterogeneous catalytic system for direct hydroxylation of haloarenes 显示文摘 | Amal Joseph P J Priyadarshini S Lakshmi Kantam M | 2011 | Catal Sci & Techno2011，4，： | 1 |
| 17 | Synthesis of a novel magnetic photocatalyst by direct deposition of nanosized TiO2 crystals onto a magnetic core显示文摘 | WATSON S BEYDOUN D AMAL A | 2002 | J Photochem Photobiol A:Chem2002，148，： | 1 |
| 18 | The effect of floc size and structure on specific cake resistance and compressibility in dead - end microfiltration 显示文摘 | Lee S A Fane A G Amal R | 2003 | Sep Sci and Technol2003，38，4： | 1 |
| 19 | Synthesis of a novel magnetic photocatalyst by direct deposition of nanosized TiO2 crystals onto a magnetic core 显示文摘 | WATSON S BEYDOUN D AMAL R | 2002 | Journal of Photochemistry and Photobiology A:Chemistry2002，，148： | 1 |
| 20 | Efficacy and safety of deferasirox,an oral iron chelator,inheavily iron-overloaded patients with b-thalassaemia:the ESCALATOR study显示文摘 | Ali Taher1 Amal El-Beshlawy Mohsen S | | Journal Compilation0，82，6： | 1 |
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