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| 1 | Efficient Gene Targeting in Zebrafish Mediated by a Zebrafish-Codon-Optimized Cas9 and Evaluation of Off-Targeting Effect显示文摘Targeted genome modifications with the Cas9/gRNA system derived from the clustered regularly interspaced short palindromic repeat/CRISPR-associated(CRISPR/Cas)system have been successfully used in cultured human cells as well as in most model organisms,including zebrafish(Danio rerio),mouse,and fruit fly(Chang et al.,2013;Cong et al.,2013; | Da Liu Zhanxiang Wang An Xiao Yutian Zhang Wenyuan Li Yao Zu Shaohua Yao Shuo Lin Bo Zhang | 2014 | Journal of Genetics and Genomics2014，41，1： | 18 |
| 2 | A Novel Semiconductor CIGS Photovoltaic Material and Thin-Film ED Technology显示文摘In order to achieve low cost high efficiency thin film solar cells,a novel Semiconductor Photovoltaic (PV) active material CuIn 1-x Ga x Se 2 (CIGS) and thin film Electro Deposition (ED) technology is explored.Firstly,the PV materials and technologies is investigated,then the detailed experimental processes of CIGS/Mo/glass structure by using the novel ED technology and the results are reported.These results shows that high quality CIGS polycrystalline thin films can be obtained by the ED method,in which the polycrystalline CIGS is definitely identified by the (112),(204,220) characteristic peaks of the tetragonal structure,the continuous CIGS thin film layers with particle average size of about 2μm of length and around 1 6μm of thickness.The thickness and solar grade quality of CIGS thin films can be produced with good repeatability.Discussion and analysis on the ED technique,CIGS energy band and sodium (Na) impurity properties,were also performed.The alloy CIGS exhibits not only increasing band gap with increasing x ,but also a change in material properties that is relevant to the device operation.The beneficial impurity Na originating from the low cost soda lime glass substrate becomes one prerequisite for high quality CIGS films.These novel material and technology are very useful for low cost high efficiency thin film solar cells and other devices. | ZHENG Guang fu 1,YANG Hong xing 1,MAN Cheuk ho 1,WONG Wing lok 2, AN Da wei 1 and John BURNETT 1(1 Centre for Development of Solar Energy Technology,Department of Building Services Engineering, The Hong Kong Polytechnic University,Hong Kong,C | 2001 | Journal of Semiconductors2001，22，11： | 10 |
| 3 | Two New Flavonoid Glycosides from Chrysanthemum morifolium显示文摘Two new flavonoid glycosides were isolated from the flowering heads of Chrysanthemum morifolium. Their structures were determined to be luteolin 4’-methoxy-7- O-(6'-O-acetyl)-β-D-glucopyranoside (1) and acacetin 7-O-(3'-O-acetyl)-β-D-glucopyranoside (2) by means of 1H and 13C NMR spectroscopic analysis, including 2D NMR technique. | Jian ZHANG An Wei DING You Bin LI Da Wei QIAN Jin Ao DUAN Zhi Qi YIN | 2006 | Chinese Chemical Letters2006，17，8： | 10 |
| 4 | Advanced data analytics for enhancing building performances: From data-driven to big data-driven approaches显示文摘Buildings have a significant impact on global sustainability.During the past decades,a wide variety of studies have been conducted throughout the building lifecycle for improving the building performance.Data-driven approach has been widely adopted owing to less detailed building information required and high computational efficiency for online applications.Recent advances in information technologies and data science have enabled convenient access,storage,and analysis of massive on-site measurements,bringing about a new big-data-driven research paradigm.This paper presents a critical review of data-driven methods,particularly those methods based on larger datasets,for building energy modeling and their practical applications for improving building performances.This paper is organized based on the four essential phases of big-data-driven modeling,i.e.,data preprocessing,model development,knowledge post-processing,and practical applications throughout the building lifecycle.Typical data analysis and application methods have been summarized and compared at each stage,based upon which in-depth discussions and future research directions have been presented.This review demonstrates that the insights obtained from big building data can be extremely helpful for enriching the existing knowledge repository regarding building energy modeling.Furthermore,considering the ever-increasing development of smart buildings and IoT-driven smart cities,the big data-driven research paradigm will become an essential supplement to existing scientific research methods in the building sector. | Cheng Fan Da Yan Fu Xiao Ao Li Jingjing An Xuyuan Kang | 2021 | Building Simulation2021，14，1： | 10 |
| 5 | Mechanism of exogenous nucleic acids and their precursors improving the repair of intestinal epithelium after 7-irradiation in mice显示文摘AIM To clone expressed genes associated withrepair of irradiation-damaged mice intestinalgland cells treated by small intestinal RNA,andto explore the molecular mechanism ofexogenous nucleic acids improving repair ofintestinal crypt.METHODS The animal mode of test group andcontrol group was established,forty-five micebeing irradiated by γ ray were treated with smallintestinal RNA as test group,forty mice beingirradiated by γ ray were treated withphysiological saline as control group,five micewithout irradiation were used as normal control,their jejunal specimens were collectedrespectively at 6h,12h,24h,4d and 8d afterirradiation.Then by using LD-PCR based onsubtractive hybridization,these gene fragmentsdifferentially expressed between test group andcontrol group were obtained,and then werecloned into T vectors as well as beingsequenced.Obtained sequences were screenedagainst.GeneBank,if being new sequences,they were submitted to GeneBank.RESULTS Ninety clones were associated withrepair of irradiation-damaged intestinal glandcells treated by intestinal RNA.These clonesfrom test group of 6h,12h,24h,4d and 8dwere respectively 18,22,25,13,12.By screening against GeneBank,18 of which werenew sequences,the others were dramaticallysimilar to the known sequences,mainly similarto hsp,Nmi,Dutt1,alkaline phosphatase,homeobox,anti-CEA ScFv antibody,arginine/serine kinase and BMP-4,repA.Eighteen genefragments were new sequences,their acceptnumbers in GeneBank were respectivelyAF240164-AF240181.CONCLUSION Ninety clones were obtained tobe associated with repair of irradiation-damagedmice intestinal gland cells treated by smallintestinal RNA,which may be related toabnormal expression of genes and matchedproteins of hsp,Nmi,Duttl,Na,K-ATPase,alkalineph-osphatase,glkA,single strandedreplicative centromeric gene as well as 18 newsequences. | Da Xiang Cui~1 Guei Ying Zeng~2 Feng Wang~1 Jun Rong Xu~1 Dong Qing Ren~2 Yan Hai Guo~1 Fu Rong Tian~2 Xiao Jun Yan~1 Yu Hou~1 Cheng Zhi Su~1 1 Institute of Genetic Diagnosis of the Fourth Military Medical University,Xi’an 710032,China 2 Department of Irradiation Medicine of the Fourth Military Medical University,Xi’an 710032,China | 2000 | World Journal of Gastroenterology2000，6，5： | 8 |
| 6 | Dynamic analysis of granite rockburst based on the PIV technique显示文摘This paper describes the deep rockburst simulation system to reproduce the granite instantaneous rockburst process.Based on the PIV(Particle Image Velocimetry)technique,quantitative analysis of a rockburst,the images of tracer particle,displacement and strain fields can be obtained,and the debris trajectory described.According to the observation of on-site tests,the dynamic rockburst is actually a gas–solid high speed flow process,which is caused by the interaction of rock fragments and surrounding air.With the help of analysis on high speed video and PIV images,the granite rockburst failure process is composed of six stages of platey fragment spalling and debris ejection.Meanwhile,the elastic energy for these six stages has been calculated to study the energy variation.The results indicate that the rockburst process can be summarized as:an initiating stage,intensive developing stage and gradual decay stage.This research will be helpful for our further understanding of the rockburst mechanism. | Wang Hongjian Liu Da'an Gong Weili Li Liyun | 2015 | International Journal of Mining Science and Technology2015，25，2： | 6 |
| 7 | A data-driven model predictive control for lighting system based on historical occupancy in an office building: Methodology development显示文摘The lighting system accounts for 8%of the total electricity consumption in commercial buildings in the United States and 12%of the total electricity consumption in public buildings globally.This consumption share can be effectively reduced using the demand-response control.The traditional lighting system control method commonly depends on the real-time occupancy data collected using the passive infrared(PIR)sensor.However,the detection inaccuracy of the PIR sensor usually results in false-offs.To diminish the false-error frequency,the existing lighting system control simply deploys a delayed reaction period(e.g.,5 to 20 min),which is not sufficiently accurate for the demand-response operation.Therefore,in this research,a novel data-driven model predictive control(MPC)method that is based on the temporal sequential-based artificial neural network(TS-ANN)is proposed to overcome this challenge using an updated historical occupancy status.Using an office as case study,the proposed model is also compared with the traditional lighting system control method.In the proposed model,the occupancy data was trained to predict the occupancy pattern to improve the control.It was found that the occupancy prediction mainly correlates with the historical occupancy ratio and the time sequential feature.The simulation results indicated that the proposed method achieved higher accuracy(97.4%)and fewer false-offs(from 79.5 with traditional time delay method to 0.6 times per day)are achieved by the MPC model.The proposed TS-ANN-MPC method integrates the analysis of the occupant behavior routine into on-site control and has the potential to further enhance the control performance practice for maximum energy conservation. | Yuan Jin Da Yan Xingxing Zhang Jingjing An Mengjie Han | 2021 | Building Simulation2021，14，1： | 6 |
| 8 | Regulatory effects of electro-acupuncture at Zusanfi on ir-SP content in rat pituitary gland and peripheral blood and their immunity显示文摘INTRODUCTIONIt has been reported in many studies that electro-acupuncture(EA)can positively regulate erythrocyticimmunity and T-lymphocytic subgroups.Nevertheless,its mechanism remains to be explored.In thepresent study,a multi-group,multi-stepped and multi-indexed observation was conducted on the effects of EA | Wei Gao Yu Xin Huang Hong Chen Da Yong Song Qin Li Wang Department of Gastroenterology Medical Affair Office,Tangdu Hospital,4th Military Medical University,Xi’an 710038,Shanxi Province,China Department of Gastroenterology,Chinese PLA General Hospital of Guangzhou Command Area,Guangzhou 510210,Guangdong Province,China | 2000 | World Journal of Gastroenterology2000，6，4： | 5 |
| 9 | DeST 3.0:A new-generation building performance simulation platform显示文摘Buildings contribute to almost 30%of total energy consumption worldwide.Developing building energy modeling programs is of great significance for lifecycle building performance assessment and optimization.Advances in novel building technologies,the requirements of high-performance computation,and the demands for multi-objective models have brought new challenges for building energy modeling software and platforms.To meet the increasing simulation demands,DeST 3.0,a new-generation building performance simulation platform,was developed and released.The structure of DeST 3.0 incorporates four simulation engines,including building analysis and simulation(BAS)engine,HVAC system engine,combined plant simulation(CPS)engine,and energy system(ES)engine,connected by air loop and water loop balancing iterations.DeST 3.0 offers numerous new simulation features,such as advanced simulation modules for building envelopes,occupant behavior and energy systems,cross-platform and compatible simulation kernel,FMI/FMU-based co-simulation functionalities,and high-performance parallel simulation architecture.DeST 3.0 has been thoroughly evaluated and validated using code verification,inter-program comparison,and case-study calibration.DeST 3.0 has been applied in various aspects throughout the building lifecycle,supporting building design,operation,retrofit analysis,code appliance,technology adaptability evaluation as well as research and education.The new generation building simulation platform DeST 3.0 provides an efficient tool and comprehensive simulation platform for lifecycle building performance analysis and optimization. | Da Yan Xin Zhou Jingjing An Xuyuan Kang Fan Bu Youming Chen Yiqun Pan Yan Gao Qunli Zhang Hui Zhou Kuining Qiu Jing Liu Yan Liu Honglian Li Lei Zhang Hong Dong Lixin Sun Song Pan Xiang Zhou Zhe Tian Wenjie Zhang Ruhong Wu Hongsan Sun Yu Huang Xiaohong Su Yongwei Zhang Rui Shen Diankun Chen Guangyuan Wei Yixing Chen Jinqing Peng | 2022 | Building Simulation2022，15，11： | 5 |
| 10 | A new metabonomics method for simultaneous determination of EFAs and NEFAs in plasma using GC-MS and its application显示文摘A new metabonomics method was developed for simultaneous qualitative and quantitative analysis on esterified and nonesterified fatty acids(EFAs and NEFAs) in plasma.Merely 10μL of plasma was required.The pretreatment of the sample was simple without disposing the protein.After simple extraction and derivation,15 FAs in plasma were precisely quantified.Gas chromatography tandem mass spectrometry(GC-MS) was used in the study and the quantities of the analytes,which varied in abundance over three orders of magnitude.The established method showed good accuracy.The curve correlation coefficients were all above 0.997.The RSDs of precision for all compounds were below 15%and recoveries were all between 80 and 110%.PLS-DA modal was used in the data management. | Li Da Han Qiong Lin Liang Yi Ming Wang Ping Hu Guo An Luo | 2009 | Chinese Chemical Letters2009，20，9： | 5 |
| 11 | An Effective Over-sampling Method for Imbalanced Data Sets Classification显示文摘 | ZHAI Yun MA Nan RUAN Da AN Bing | 2011 | Chinese Journal of Electronics2011，20，3： | 5 |
| 12 | Metabolism Study on Tobacco-specific N-Nitrosamines in Rabbit by Solid-phase Extraction and Liquid Chromatography-electrospray Ionization-mass Spectrometry Method显示文摘Metabolism of four tobacco-specific N-nitrosamines (TSNAs), N′-nitrosonornicotine (NNN), N′-nitrosoanatabine (NAT), N′-nitrosoanabasine (NAB) and 4-(methyl nitrosamino)-1- (3-pyridyl)-1-butanone (NNK) was studied by solid-phase extraction (SPE) and liquid chromato- graphy-electrospray ionization-mass spectrometry (LC-ESI-MS). SPE and LC-ESI-MS method was evaluated to be rapid, simple, sensitive and selective for analysis of TSNAs in rabbit serum. The doses of TSNAs administrated were 4.666 μgkg and 11.665 μgkg according to different levels in cigarettes. Metabolic curves of four TSNAs and 4-(methyl nitrosamino)-1-(3-pyridyl)- 1-butanone (NNAL), the metabolite of NNK, were obtained. | Jian Xun ZHANG Chen Chen LI Zheng CHEN Zhi Hua RAO Da Wei WEN Ke An LI Hu Wei LIU | 2005 | Chinese Chemical Letters2005，16，12： | 5 |
| 13 | Analysis for remedial alternatives of unregulated municipal solid waste landfills leachate-contaminated groundwater显示文摘地下水流动和溶质运输模型为预报沾染物运输并且基于 unregulated 垃圾堆的案例研究估计补救选择的效果用视觉 Modflow 被开发在东方中国的污染 leachate 的地下水。结果证明那根砷羽毛是到达在八年,和砷羽毛的最长侧面、纵的距离以后在下游的农田抽很好是分别地到达 200 m 和 260 m。但是砷羽毛的区域是不显然从八年增加到十年和羽毛的高集中的区域是在 20 年以后传播到下游的河和农田区域;当垃圾堆磨擦了时被变硬,羽毛是不在八年以后到达下游的农田区域;当抽的井下游地在羽毛被安装,分泌物率是 200m ^ 时,啜 3 ^/d,羽毛将有效地被制止;为防漏的障碍,它可能有效地在一个程度时间范围以内保护敏感目标的地下水。要不是连续的点来源,羽毛仍然是防漏的障碍到圆;当排水阴沟的分泌物率是 170.26 m ^ 时,啜 3 ^/d,羽毛有效地被控制;有排水阴沟的联合地面变硬能在控制沾染物散开给最好的效果的全面方法,和分泌物率将被归结为 111.43 m ^ 啜 3 ^/d。因此,当 leachate 漏发生在 unregulated 垃圾堆时,有排水阴沟的全面补救其他的联合地面变硬将为阻止地下水污染被推荐[出版摘要] | Da AN Yonghai JIANG Beidou XI Zhifei MA Yu YANG Queping YANG Mingxiao LI Jinbao ZHANG Shunguo BAI Lei JIANG | 2013 | Frontiers of Earth Science2013，7，3： | 3 |
| 14 | Effects of IκBα and its mutants on NF-κB and p53 signaling pathways显示文摘AIM: To study the effects of IκBα and its mutants (IκBαM, IκBα243N, IκBαM244C) on NF-κB, p53 and their downstream target genes. The relationship of NF- κB, p53, and IκBα was further discussed.METHODS: pECFP-IκBα, pECFP-IκBαM (amino acides 1-317, Ser32, 36A), pECFP-IκBα243N (amino acides 1-243), pECFP-IκBα244C (amino acides 244-317), pEYFP-p65 and pp53-DsRed were constructed and transfected to ASTC-α-1 cells. Cells were transfected with pECFP-C1 as a control. 30 h after the transfection, location patterns of NF-κB, p53 and IκBα (IκBαM, IκBα243N, IκBα244C) were observed by a laser scanning microscope (LSM510/ConfoCor2, Zeiss). RNA extraction and reverse transcription were performed in cells transfected or co-transfected with different plasmids. Effects of IκBα and its mutants on the transprition level of NF-κB, NF-κB downstream target gene TNF-α, p53 and p53 downstream target gene Bax were observed by real time QT-PCR. In all experiments β-actin was reference. Results are expressed as the target/reference ratio of the sample divided by the target/reference ratio of the control. Different transfected cells were incubated with CCK-8 for 2 h in the incubator. Then the absorbance at 450 nm was measured by using a microplate reader. RESULTS: Cells that were transfected with p53- DsRed revealed a predominant nuclear localization. YFP-p65 mainly existed in the cytoplasm. Cells were transfected with CFP-IκBα, CFP-IκBαM, and CFP- IκBα243N respectively and revealed a predominant cytosolic localization. However, cells transfected of CFP- IκBα244C revealed a predominant nuclear localization. The mRNA levels of p65, TNF-α, p53 and Bax in CFP-IκBα transfected cells did not change significantly, while in YFP-p65/CFP-IκBα co-transfected cells, IκBα decreased the transcription of p65 downstream gene TNF-α (2.24 ± 0.503) compared with the YFP-p65/ CFP-C1 co-transfected cells (5.08 ± 0.891) (P < 0.05). Phosphorylation defective IκBα (IκBαM) decreased the transcription levels of all the four genes compared with the control (P < 0.05). The N terminus of IκBα (IκBα243N) increased the transcription of NF-κB (1.84 ± 0.176) and TNF-α (1.51 ± 0.203) a little bit. However, the C terminus of IκBα (IκBα244C) increased the transcription of NF-κB, TNF-α, p53 and Bax significantly (8.29 ± 1.662, 14.16 ± 2.121, 10.2 ± 0.621, 3.72 ± 0.346) (P < 0.05). The CCK-8 experiment also showed that IκBα244C and p53 synergistically mediate apoptosis. CONCLUSIONS: IκBα and its mutants (IκBαM, IκBα243N, IκBαM244C) have different effects on NF- κB and p53 signaling pathways, according to their different structures. IκBαM bounds with NF-κB and p53 in cytoplasm steadily, and inhibits both of the two signaling pathways. p53 and IκBα244C may be co-factor in inducing apoptosis. The C terminal of IκBα enhanced cell death, which suggests that it may be a pro-apoptotic protein existed in cells. | Xian Li Da Xing Ju Wang De-Bin Zhu Lan Zhang Xiao-Jia Chen Fen-Yong Sun An Hong | 2006 | World Journal of Gastroenterology2006，12，41： | 3 |
| 15 | Development and Evaluation of a Distance Learning System Based on CSCW显示文摘This paper described a distance learning system, which allows Internet users to conduct a lesson in real time from any kinds attached computers. Participants can jointly view and edit relevant multimedia information distributed through Internet. Teachers and students can also simultaneously communicate by voice and text to discuss the problems. Teacher can broadcast streaming PowerPoint presentation in real time to network users. In addition to sliders, presenters can broadcast video and audio simultaneously to deliver a live multimedia show online, and store their presentations for on demand playback. Teachers distributed in different places can also use cooperative editing tool to edit and encode existing digital content. We discussed some important design principles of the system. Then, the system configuration and the results of evaluation are also presented. The system has proved to be applicable to the distance learning based on CSCW (Computer Support Cooperative Work) in Internet. | Yin Hao,Zhu Guang\|xi,Li Xiao\|long,Zhu Yao\|ting,He Da\|an Electronic Engineering Department,Huazhong University of Science and Technology , Wuhan 430074,China | 2001 | Wuhan University Journal of Natural Sciences2001，6，Z1： | 2 |
| 16 | Rare pathological variants and presentations of primary central nervous system lymphomas显示文摘 | Da Silva AN Lopes MB Schiff D | 2006 | Neurosurg Focus2006，1521，5： | 1 |
| 17 | Sleep apnea and daytime sleepiness and fatigue: relation to visceral obesity, insulin resistance, and hypercytokinemia显示文摘 | Vgontzas AN Papanicolaou DA Bixler EO | 2000 | J Clin Endocrinol Metab2000，85，3： | 1 |
| 18 | Sleep apnea and daytime sleepiness and fatigue : relation to visceral obesity, insulin resistance, and hypercytokinemia 显示文摘 | Vgontzas AN Papanicolaou DA Bixler EO | 2000 | J Clin Endocrinol Metab2000，85，3： | 1 |
| 19 | Upper urinary tract transitional cell carcinoma: current treatment overview of minimally invasive approaches显示文摘 | Argyropoulos AN Tolley DA | 2007 | BJU Int2007，99，5： | 1 |
| 20 | Results of a rapid HIVscreening and diagnostic testing program in an urban emergency depart-ment 显示文摘 | White DA Scribner AN Schulden JD | 2009 | Ann Emerg Med2009，54，1： | 1 |
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