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| 1 | Resveratrol and fenofibrate ameliorate fructose-induced nonalcoholic steatohepatitis by modulation of genes expression显示文摘AIM: To evaluate the effect of resveratrol, alone and in combination with fenofibrate, on fructose-induced metabolic genes abnormalities in rats. METHODS: Giving a fructose-enriched diet(FED) to rats for 12 wk was used as a model for inducing hepatic dyslipidemia and insulin resistance. Adult male albino rats(150-200 g) were divided into a control group and a FED group which was subdivided into 4 groups, a control FED, fenofibrate(FENO)(100 mg/kg), resveratrol(RES)(70 mg/kg) and combined treatment( FENO + RES)( half the doses). A l l treatments were given orally from the 9th week till the end of experimental period. Body weight, oral glucose tolerance test(OGTT), liver index, glucose, insulin, insulin resistance(HOMA), serum and liver triglycerides(TGs), oxidative stress(liver MDA, GSH and SOD),serum AST, ALT, AST/ALT ratio and tumor necrosis factor-α(TNF-α) were measured. Additionally, hepatic gene expression of suppressor of cytokine signaling-3(SOCS-3), sterol regulatory element binding protein-1c(SREBP-1c), fatty acid synthase(FAS), malonyl Co A decarboxylase(MCD), transforming growth factor-β1(TGF-β1) and adipose tissue genes expression of leptin and adiponectin were investigated. Liver sections were taken for histopathological examination and steatosis area were determined.RESULTS: Rats fed FED showed damaged liver, impairment of glucose tolerance, insulin resistance, ox ida t ive s t re s s a nd dy s l ipide m ia. As fo r ge ne expression, there was a change in favor of dyslipidemia and nonalcoholic steatohepatitis(NASH) development. All treatment regimens showed some benefit in reversing the described deviations. Fructose caused deterioration in hepatic gene expression of SOCS-3, SREBP-1c, FAS, MDA and TGF-β1 and in adipose tissue gene expression of leptin and adiponectin. Fructose showed also an increase in body weight, insulin resistance(OGTT, HOMA), serum and liver TGs, hepatic MDA, serum AST, AST/ALT ratio and TNF-α compared to control. All treatments improved SOCS-3, FAS, MCD, TGF-β1 and leptin genes expression while only RES and FENO + RES groups showed an improvement in SREBP-1c expression. Adiponectin gene expression was improved only by RES. A decrease in body weight, HOMA, liver TGs, AST/ALT ratio and TNF-α were observed in all treatment groups. Liver index was increased in FENO and FENO + RES groups. Serum TGs was improved only by FENO treatment. Liver MDA was improved by RES and FENO + RES treatments. FENO + RES group showed an increase in liver GSH content.CONCLUSION: When resveratrol was given with half the dose of fenofibrate it improved NASH-related fructose-induced disturbances in gene expression similar to a full dose of fenofibrate. | Enas A Abd El-Haleim Ashraf K Bahgat Samira Saleh | 2016 | World Journal of Gastroenterology2016，22，10： | 5 |
| 2 | Flow cytometric detection of hepatitis C virus antigens in infected peripheral blood leukocytes: Binding and entry显示文摘AIM: We designed two synthetic-core-specific peptides core 1 (C1) and core 2 (C2), and an E1-specific peptide (E1). We produced specific polyclonal antibodies againstthese peptides and used the antibodies for detection of HCV antigens on surface and within infected peripheral blood leukocytes.METHODS: Peripheral blood from a healthy individual who tested negative for HCV RNA was incubated with HCV type 4 infected serum for 1 h and 24 h at 37 ℃. Cells were stained by direct and indirect immunofluorescence and measured by flow cytometry.RESULTS: After 1 h of incubation, antibodies against C1,C2, and E1 detected HCV antigens on the surface of 27%,26% and 73% of monocytes respectively, while 10%, 5% and 9% of lymphocytes were positive with anti-C1, anti-C2 and anti-E1 respectively. Only 1-3% of granulocytes showed positive staining with anti-C1, anti-C2 and anti E1 antibodies. After 24 h of incubation, we found no surface staining with anti-C1, anti-C2 or anti-E1. Direct immunostaining using anti-C2 could not detect intracellular HCV antigens, after 1 h of incubation with the virus, while after 24 h of incubation, 28% of infected cells showed positive staining. Only plus strand RNA was detectable intracellularly as early as 1 h after incubation, and remained detectable throughout 48 h post-infection.Interestingly, minus RNA strand could not be detected after 1 h, but became strongly detectable intracellularly after 24 h post-infection.CONCLUSION: Monocytes and lymphocytes are the preferred target cells for HCV infection in peripheral blood leukocytes. Our specific anti-core and anti-E1 antibodies are valuable reagents for demonstration of HCV cell cycle.Also, HCV is capable of infecting and replicating in peripheral blood mononuclear cells as confirmed by detection of minus strand HCV RNA as well as intracellular staining of core HCV antigen. | Mostafa K El-Awady Ashraf A Tabll El-Rashdy M Redwan Samar Youssef Moataza H Omran Fouad Thakeb Maha El-Demellawy | 2005 | World Journal of Gastroenterology2005，11，33： | 4 |
| 3 | HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro显示文摘瞄准:与丙肝的长期的复制建立一个房间文化系统病毒(HCV ) 染色体和病毒的抗原的表示在试管内。方法:HepG2 房间线被孵化与长期的丙肝从一个病人与浆液为它的危险性测试到 HCV。房间和上层清液在文化期间在各种各样的时间点被收获。文化上层清液为它感染天真的房间的能力被测试。存在减(反感觉) 在房间的核心和 E1 抗原的 RNA 海滨,和察觉被 RT-PCR 和免疫学的技术(流动血细胞计数和西方的污点) 分别地检验。结果:细胞内部的 HCV RNA 首先在 d 上被检测 3 在感染以后然后能一致地在至少三个月的一个时期上在房间和上层清液被检测。新鲜房间能从有教养的感染的房间感染上层清液。流动 cytometric 分析证明表面和在房子里使用的细胞内部的 HCV 抗原表示使 polyclonal 成为了抗体(反核心,和 anti-E1 ) 。西方的污点分析证明在分子量的产生免疫性的肽的簇的表示在一个月内在 31 和 45 kDa 之间延长了感染的房间的旧文化而这簇在 uninfected HepG2 房间是无法发现的。结论:HepG2 房间线产生 HCV 感染而且支持它的复制在试管内不仅。HCV 结构的蛋白质的表示能在感染的 HepG2 房间被检测。这些房间也能够流病毒的粒子进接着对 uninfected 房间变得传染的培养基。 | Mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy M Redwan Maha El-Demellawy Moataza H Omran Wael T El-Garf Said A Goueli | 2006 | World Journal of Gastroenterology2006，12，30： | 2 |
| 4 | Impulse oscillometry in the evaluation of diseases of the airways in children 显示文摘 | Hirsh D K Ian A M Ashraf U | 2011 | Ann Allergy Asthma Immunol2011，106，3： | 1 |
| 5 | Recent advances in silicon etching for MEMS using the ASETM process 显示文摘 | HYNES A M ASHRAF H BHARDWAJ J K | 1999 | Sensors and Actuators A : Physical1999，74，13： | 1 |
| 6 | 2, 3-Dichloro-5, 6-Dicyano-p Benzoquinone (DDQ) as a Highly Efficient and Mild Catalyst for Diethyl Acetalization of Carbonyl Compounds 显示文摘 | BABAK K ASHRAF M A | 1999 | Chem Lett1999，，： | 1 |
| 7 | Hierarchical segmentationbased image coding using hybrid quad-binary trees 显示文摘 | ASHRAF A K WEI S L ZONOOBI D | 2009 | IEEE Trans on Image Processing2009，18，6： | 1 |
| 8 | Detectionand characterization of virulence genes and integrons inAeromonas veronii isolated from catfish 显示文摘 | Mohamed N Saeed A K Ashraf A K | 2010 | Food Mi-crobiol2010，27，3： | 1 |
| 9 | Treatment of experimentalosteomyelitis by liposomal antibiotics 显示文摘 | Ashraf A K Saleh A A Adel R A | 2004 | Journal of AntimicrobialChemotherapy2004，54，： | 1 |
| 10 | Threshold for effects of vitamin D deficiency on glucose metabolism in obese female African-American adolescents显示文摘 | Ashraf A Alvarez J Saenz K | 2009 | J Clin Endocrinol Metab2009，94，9： | 1 |
| 11 | Identification andcharacterization of class 1 integron resistance gene cassettes amongSalmonella strains isolated from imported seafood显示文摘 | Ashraf A K Elizabeth P Nawaz M S | 2009 | Appliedand Environmental Microbiology2009，75，4： | 1 |
| 12 | Prevalence and risk factors of Otitis Media with effusion in school children in Qassim Region of Saudi Arabia显示文摘 | Humaid A H Ashraf A H Masood K A | 2014 | Int J Health Sci( Qassim )2014，8，32： | 1 |
| 13 | Impact of interfering bluetooth piconets on a collocated p persistent CSMA-based WLAN显示文摘 | Ashraf I Voulgaris K Gkelias A | 2009 | IEEE Transactions on Vehicular Technology2009，58，9： | 1 |
| 14 | Relationship between ion accumulation and growth in two springwheat lines differing in salt tolerance at different growth stages显示文摘 | ASHRAF M AASIYA K KHANUM A | 1997 | Journal of Agronomy and Crop Science1997，178，： | 1 |
| 15 | Factors delaying hospital arrival of patients with acute stroke显示文摘 | V Ashraf M Maneesh R Praveenkumar K Saifudheen A Girija | 2015 | Annals of Indian Academy of Neurology2015，，： | 1 |
| 16 | Hyperimmune bovine colostrum in the treatment of shigellosis in children: a double-blind, randomized, controlled trial 显示文摘 | Ashraf H Mahalanabis D Mitra A K | 2001 | Acta Paediatrica2001，90，12： | 1 |
| 17 | In-situ observation of deep water corals in the northern Red Sea waters of Saudi Arabia显示文摘 | QURBAN M A KRISHNAKUMAR P K JOYDAS T V MANIKANDAN K P ASHRAF T T M QUADRI S 1 WAFAR M QASEM A CAIRNS S D | 2014 | Deep Sea Research Part I: Oceanographic Research Papets2014，89，： | 1 |
| 18 | Hyperimmune bovine colostrum in the treatment of shigellosis in children : A double-blind, randomized, controlled trial 显示文摘 | Ashraf H Mahalanabis D Mitra A K | 2001 | Acta Paediatrica2001，90，： | 1 |
| 19 | Effect of lime treatment of olive meal on in vitro utilization of total mixed ration containing olive meal as partial maize replacer显示文摘 | ASHRAF A SHARMA R K RASTOGI A | 2013 | Veterinary World2013，6，： | 1 |
| 20 | Threshold for effects of vitamin d deficiency on glucose metabolism in obsess female African-american adolescents显示文摘 | Ashraf A Alvarez J Saenz K | 2009 | J Clin Endocrinol Metab2009，94，32： | 1 |
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