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| 1 | Sodium glucose co-transporter 2 inhibition reduces succinate levels in diabetic mice显示文摘BACKGROUND Type 1 diabetes(T1D) is associated with major chronic microvascular complications which contribute significantly to diabetes associated morbidity.The protein primarily responsible for glucose reabsorption in the kidney is sodium glucose co-transporter 2(SGLT2). Presently, SGLT2 inhibitors are widely used in diabetic patients to improve blood glucose levels and prevent cardiovascular and renal complications. Given the broad therapeutic application of SGLT2 inhibitors, we hypothesised that SGLT2 inhibition may exert its protective effects via alterations of the gut microbiome and tested this in a type 1 diabetic mouse model of diabetic retinopathy.AIM To determine whether the treatment with two independent SGLT2 inhibitors affects gut health in a type 1 diabetic mouse model.METHODS The SGLT2 inhibitors empagliflozin or dapagliflozin(25 mg/kg/d) or vehicle dimethylsulfoxide(DMSO) were administered to C57 BL/6 J, Akita, Kimba and Akimba mice at 10 wk of age for 8 wk via their drinking water. Serum samples were collected and the concentration of succinate and the short chain fatty acid(SCFA) butyric acid was measured using gas chromatography-mass spectrometry. Enzyme-linked immunosorbent assay(ELISA) was performed to determine the concentration of insulin and leptin. Furthermore, the norepinephrine content in kidney tissue was determined using ELISA. Pancreatic tissue was collected and stained with haematoxylin and eosin and analysed using brightfield microscopy.RESULTS Due to the presence of the Akita allele, both Akita and Akimba mice showed a reduction in insulin production compared to C57 BL/6 J and Kimba mice.Furthermore, Akita mice also showed the presence of apoptotic bodies within the pancreatic islets. The acinar cells of Akita and Akimba mice showed swelling which is indicative of acute injury or pancreatitis. After 8 wk of SGLT2 inhibition with dapagliflozin, the intermediate metabolite of gut metabolism known as succinate was significantly reduced in Akimba mice when compared to DMSO treated mice. In addition, empagliflozin resulted in suppression of succinate levels in Akimba mice. The beneficial SCFA known as butyric acid was significantly increased in Akita mice after treatment with dapagliflozin when compared to vehicle treated mice. The norepinephrine content in the kidney was significantly reduced with both dapagliflozin and empagliflozin therapy in Akita mice and was significantly reduced in Akimba mice treated with empagliflozin.In non-diabetic C57 BL/6 J and Kimba mice, serum leptin levels were significantly reduced after dapagliflozin therapy.CONCLUSION The inhibition of SGLT2 reduces the intermediate metabolite succinate, increases SCFA butyric acid levels and reduces norepinephrine content in mouse models of T1 D. Collectively, these improvements may represent an important mechanism underlying the potential benefits of SGLT2 inhibition in T1 D and its complications. | Lakshini Y Herat Natalie C Ward Aaron L Magno Elizabeth P Rakoczy Marcio G Kiuchi Markus P Schlaich Vance B Matthews | 2020 | World Journal of Gastroenterology2020，26，23： | 3 |
| 2 | Capillary electrophoresis of free fatty acids by indirect uhraviolet 显示文摘 | Maria V B Aaron E D Maria J L G | 2011 | Journal of Agriculture and Food Chemistry2011，59，10： | 1 |
| 3 | Use of amino-terminal pro-B-type natriuretic peptide to guide outpatient therapy of patients with chronic left ventricular systolic dysfunction 显示文摘 | JAMES L JANUZZI JR MD SHAFIQ U REHMAN M D ASIM A MOHAMMED M D ANJU BHARDWAJ M D LINDA BARAJAS R N JUSTINE BARAJAS HAN-NA KIM M D MPH AARON L BAGGISH M D RORY B WEINER M D ANNABEL CHEN-TOURNOUX MD JANE E MARSHALL RDCS STEPHANIE A MOORE M D WILLIAM D CARLSON M D GREGORY D LEWIS M D JORDAN SHIN M D DOROTHY SULLIVAN ANP KIMBERLY PARKS D O THOMAS J WANG M D SHAWN A GREGORY M D SHANMUGAM UTHAMALINGAM M D MARC J SEMIGRAN M D | 2011 | J Am Coll Cardiol2011，58，18： | 1 |
| 4 | Simultaneous assay of fluoroquinolones and theophyline in plasma by high-performancc liquid chromatography显示文摘 | Davis J D Aarons L Houston J B | 1993 | J Chromatogr1993，621，1： | 1 |
| 5 | Impact of family hypertension history on exercise-induced cardiac remodeling 显示文摘 | Aaron L Baggish Rory B Weiner Kibar Yared | 2009 | The American Journal of Cardiology2009，104，1： | 1 |
| 6 | The United Kingdom public health re- sponse to an imported laboratory confirmed case of a no- vel coronavirus in September 2012显示文摘 | Pebody R G Chand M A Thomas H L Green H K Boddington N L Carvalho C Brown C S Anderson S R Rooney C Crawley-Boevey E Irwin D J Aarons E Tong C Newsholme W Price N Langrish C Tucker D Zhao H Phin N Crofts J Bermingham A Gil- gunn-Jones E Brown K E Evans B Catchpole M Watson J M | 2012 | Euro Surveill2012，17，20： | 1 |
| 7 | Impact of family hypertension history on exercise-induced cardiac remodeling显示文摘 | Aaron L Baggish Rory B Weiner Kibar Yared | 2009 | The American Journal of Cardiology2009，104，1： | 1 |
| 8 | Aberrant DNA methylation in porcine in oitro-, parthenogenetic-, and somatic cell nuclear transfer-produced blastocysts显示文摘 | Aaron J B Li R F Lai L | 2008 | Molecular Reproduction and Development2008，75，2： | 1 |
| 9 | Use of biofilters and suspended growth reactors to treat VOCs显示文摘 | Aaron B N Raymond C L | 2000 | Waste Management2000，20，1： | 1 |
| 10 | Enduring effect of chronic corticosterone treatment on spatial learning,synaptic plasticity,and hippocampal neuropathology in young and mid-aged rats显示文摘 | SHARI R B AARON G H JEMMIFER C L | 1995 | J Neurosci1995，15，1： | 1 |
| 11 | Innovative Food Packaging Solutions显示文摘 | AARON L BRODDY T BETTY B | 2008 | Journal of Food Science2008，73，8： | 1 |
| 12 | Simultaneous assay of fluoroquinolones and theophylline in plasma by high-performance liquid chromatography显示文摘 | Davis J D Aarons L Houston J B | 1993 | J Chromatogr1993，621，1： | 1 |
| 13 | Synthesis and Structure of (Triphenylsilyl)imido Complexes of Titanium and Zirconium显示文摘 | LI Y H SANJUKTA B J AARON L O | 2005 | Organometallics2005，24，： | 1 |
| 14 | Renal function, congestive heart failure, and amino-terminal pro-brain natriuretic peptide measurement显示文摘 | SAIF A DONALD M L AARON B | 2006 | JACC2006，47，： | 1 |
| 15 | Amino terminal pro-brain natriuretic peptide, renal function, and outcomes in acute heart failure redefining the cardiorenal interaction? 显示文摘 | ROLAND R J JAMES L J AARON L B | 2006 | JACC2006，48，： | 1 |
| 16 | Metabolism of theophylline and its inhibition by fluoroquinolones in rat hepatic microsomes显示文摘 | Davis J D Aarons L Houston J B | 1995 | Xenobiotica1995，25，6： | 1 |
| 17 | Re-expansion pulmonary edema显示文摘 | TRACHIOTIS G D VRICELLA L A AARON B L | 1997 | Anll Tnorac Surg1997，63，4： | 1 |
| 18 | Efficiency of automation and electronic health records in optometric practice显示文摘 | Francis L M Aaron K T Aaron M B | 2008 | Optometry2008，，79： | 1 |
| 19 | Weighted neighbor-joining: a likelihood based aptnvaeh to distance based phylogeny reconstruction 显示文摘 | William J B Nicholas D S Aaron L H | 2000 | Molecular Biology and Evolution2000，17，1： | 1 |
| 20 | Reexpansion pulmonary edema显示文摘 | Mahfood S Hix W R Aaron B L | 1998 | Ann Thorac surg1998，45，： | 1 |
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