|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | YKL40 expression in CD14^+ liver cells in acute and chronic injury显示文摘AIM:To demonstrate that CD14 + cells are an important source of the growth factor YKL40 in acute and chronic liver damage.METHODS:Rats were inoculated with one dose of CCl4 to induce acute damage.Liver biopsies were obtained at 0,6,12,24,48 and 72 h.For chronic damage,CCl4 was administered three days per week for 6 or 8 wk.Tissue samples were collected,and cellular populations were isolated by liver digestion and purified by cell sorting.YKL40 mRNA and protein expression were evaluated by realtime polymerase chain reaction and western blot.RESULTS:Acute liver damage induced a rapid increase of YKL40 mRNA beginning at 12 h.Expression peaked at 24 h,with a 26fold increase over basal levels.By 72 h however,YKL40 expression levels had nearly returned to control levels.On the other hand,chronic damage induced a sustained increase in YKL40 expression,with 7and 9fold higher levels at 6 and 8 wk,respectively.The pattern of YKL40 expression in different subpopulations showed that CD14+cells,which include Kupffer cells,are a source of YKL40 after acute damage at 72 h[0.09 relative expression units(REU)]as well as after chronic injury at 6 wk(0.11 REU).Hepatocytes,in turn,accounted for 0.06 and 0.01 REU after 72 h(acute)or 6 wk(chronic),respectively.The rest of the CD14cells(including T lymphocytes,B lymphocytes,natural killer and natural killer T cells) yielded 0.07 and 0.15 REU at 72 h and 6 wk,respectively.YKL40 protein expression in liver was detected at 72 h as well as 6 and 8 wk,with the highest expression relative to controls(11fold;P≤0.05)seen at 6 wk.Macrophages were stimulated by lipopolysaccharide.We demonstrate that under these conditions,these cells showed maximum expression of YKL40 at 12 h,with P<0.05 compared with controls.CONCLUSION:Hepatic CD14 + cells are an YKL40 mRNA and protein source in acute and chronic liver injury,with expression patterns similar to growth factors implicated in inflammationfibrogenesis. | Oscar Pizano-Martínez Irinea Yaez-Sánchez Pilar Alatorre-Carranza Alejandra Miranda-Díaz Pablo C Ortiz-Lazareno Trinidad García-Iglesias Adrian Daneri-Navarro Mónica Vázquez-Del Mercado Mary Fafutis-Morris Vidal Delgado-Rizo | 2011 | World Journal of Gastroenterology2011，17，33： | 3 |
| 2 | New gene cassettes for trimethoprim resistance, dfr13 ,and Streptomycin-Spectinomytin resistance, aadA4,inserled on a class 1 integron 显示文摘 | ADRIAN P V THOMSON C J KLUGMAN K P | 2000 | Antimicrob Agents Cheroother2000，44，2： | 1 |
| 3 | Determination of critical head in soil piping显示文摘 | OJHA C S P SINGH V P ADRIAN D D | 2003 | Journal of Hydraulic Engineering2003，129，7： | 1 |
| 4 | Determination of brominated flame retardants,with emphasis on polybrominated diphenyl ethers (PBDEs) in environmental and human samples-a review显示文摘 | Adrian C Stefan V Jacob DB | 2003 | Environmet International2003，29，1： | 1 |
| 5 | Understanding how an arm swing enhances performance in the vertical jump显示文摘 | ADRIAN L JOS V DIRK D C | 2004 | J Biomech2004，37，12： | 1 |
| 6 | Chlorine dioxidedose, water quality and temperature affect the oxidative status oftomato processing water and its ability to inactivate Salmonella显示文摘 | Gabriela L V Alejandro T C Adrian S et at | 2012 | Food Control2012，26，1： | 1 |
| 7 | Determination of critical head in soil piping显示文摘 | OJHA C S P SINGH V E ADRIAN D D | 2003 | Journal of Hydraulic Engineering ASCE2003，129，7： | 1 |
| 8 | Influence of porosity on piping models of levee failure显示文摘 | OJHA C S P SINGH V P ADRIAN D D | 2001 | Journal of Geotechnical and Geoenvironmental Engineering2001，127，12： | 1 |
| 9 | pH-mediated interactions between poly (acrylic acid) and methylcellulose in the formation of ultrathin multi- layered hydrogels and spherical nanoparticles显示文摘 | Khutoryanskaya Olga V Williams Adrian C Khutoryanskiy Vitally V | 2007 | Macro- molecules2007，40，21： | 1 |
| 10 | Thermodynamic optimization of finned crossflow heat exchangers for aircraft environmental control system显示文摘 | Vargas Jose V C Bejan Adrian | 2001 | International Journal of Heat and Fluid Flow2001，22，2： | 1 |
| 11 | Overcoming drug resistance in HIV-1 chemotherapy: The binding thermo- dynamics of amprenavir and TMC-126 to wild-type and drug-resistant mutants of the HIV-1 protease 显示文摘 | Hiroyasu O Adrian V C Dong X | 2002 | Protein Sci2002，11，8： | 1 |
| 12 | Determination of brominated flame retardants, with emphasis on polybrominated diphenyl ethers (PBDEs)in environmental and human samples-a review显示文摘 | Adrian C Stefan V Jacob D B | 2003 | Environment International2003，29，1： | 1 |
| 13 | Poly ( 3-methylthiophene )/MnO2 composite electrodes as electrochemical capacitors显示文摘 | Emerson C Rios Adriane V Rosario Regina M Q Mello | 2007 | Journal of Power Sources2007，163，： | 1 |
| 14 | Determination of critical head in soil piping显示文摘 | Ojha C S P Singh V P Adrian D D | 2003 | Journal of Hydraulic Engineering2003，129，7： | 1 |
| 15 | Ioavailability and antioxidant effects of orange juice components in humans显示文摘 | Adrian A F Robert V C Susanne M H | | 0，，13： | 1 |
| 16 | Prevalence and genetic location of non-transferable trimethoprim resistant dihydrofolate reductase genes in South African commensal faecal isolates显示文摘 | Thomson C J Klugman K P | 1995 | Epidemiol Infect1995，115，2： | 1 |
| 17 | Influence of porosity on piping models of levee failure显示文摘 | OJHA C S P SINGH V P ADRIAN D D | 2001 | Journal of Geotechnical and Geoenvironmental Engineering2001，127，12： | 1 |
| 18 | Spin-on dopants as lifetime modifier sources in fast recovery epitaxial diodes processing显示文摘 | Adrian V Madalina O Dumitru C | 1997 | IEEE Trans on Electron Devices1997，97，9： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员