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| 1 | c-Fos overexpression increases the proliferation of human hepatocytes by stabilizing nuclear Cyclin D1显示文摘AIM: To investigate the effect of stable c-Fos overexpression on immortalized human hepatocyte (IHH) proliferation. METHODS: IHHs stably transfected with c-Fos (IHH-Fos) or an empty vector (IHH-C) were grown in medium supplemented with 1% serum or stimulated with 10% serum. Cell proliferation was assessed by cell counts, 3H-thymidine uptake and fl ow cytometry analyses. The levels of cell cycle regulatory proteins (Cyclin D1, E, A) cyclin dependent kinases (cdk) cdk2, cdk4, cdk6, and their inhibitors p15, p16, p21, p27, to- tal and phosphorylated GSK-3β and epidermal growth factor receptor (EGF-R) were assayed by Western blot- ting. Analysis of Cyclin D1 mRNA levels was performed by reverse transcription-polymerase chain reaction and real-time polymerase chain reaction (PCR) analysis. Stability of Cyclin D1 was studied by cycloheximide blockade experiments. RESULTS: Stable c-Fos overexpression increased cell proliferation under low serum conditions and resulted in a two-fold increase in [3H]-thymidine incorpora- tion following serum addition. Cell cycle analysis by fl ow cytometry showed that c-Fos accelerated the cell cycle kinetics. Following serum stimulation, Cyclin D1 was more abundantly expressed in c-Fos overexpressing cells. Cyclin D1 accumulation did not result from increased transcriptional activation, but from nuclear stabilization. Overexpression of c-Fos correlated with higher nuclear levels of inactive phosphorylated GSK- 3β, a kinase involved in Cyclin D1 degradation and higher levels of EGF-R mRNA, and EGF-R protein compared to IHH-C both in serum starved, and in serum stimulated cells. Abrogation of EGF-R signalling in IHH- Fos by treatment with AG1478, a specif ic EGF-R tyrosine kinase inhibitor, prevented the phosphorylation of GSK-3β induced by serum stimulation and decreased Cyclin D1 stability in the nucleus. CONCLUSION: Our results clearly indicate a positive role for c-Fos in cell cycle regulation in hepatocytes. Importantly, we delineate a new mechanism by which c-Fos could contribute to hepatocarcinogenesis through stabilization of Cyclin D1 within the nucleus, evoking a new feature to c-Fos implication in hepatocellular carcinoma. | Meryem Güller Kahina Toualbi-Abed Agnès Legrand Laurence Michel Alain Mauviel Dominique Bernuau Fanny Daniel | 2008 | World Journal of Gastroenterology2008，14，41： | 6 |
| 2 | Compared efficacy of preservation solutions on the outcome of liver transplantation:Meta-analysis显示文摘AIM To compare the effects of the four most commonly used preservation solutions on the outcome of liver transplantations.METHODS A systematic literature search was performed using MEDLINE, Scopus, EMBASE and the Cochrane Library databases up to January 31^(st), 2017. The inclusion criteria were comparative, randomized controlled trials(RCTs) for deceased donor liver(DDL) allografts with adult and pediatric donors using the gold standard University of Wisconsin(UW) solution or histidinetryptophan-ketoglutarate(HTK), Celsior(CS) and Institut Georges Lopez(IGL-1) solutions. Fifteen RCTs(1830 livers) were included; the primary outcomes were primary non-function(PNF) and one-year posttransplant graft survival(OGS-1). RESULTS All trials were homogenous with respect to donor and recipient characteristics. There was no statistical difference in the incidence of PNF with the use of UW, HTK, CS and IGL-1(RR = 0.02, 95%CI: 0.01-0.03, P = 0.356). Comparing OGS-1 also failed to reveal any difference between UW, HTK, CS and IGL-1(RR = 0.80, 95%CI: 0.80-0.80, P = 0.369). Two trials demonstrated higher PNF levels for UW in comparison with the HTK group, and individual studies described higher rates of biliary complications where HTK and CS were used compared to the UW and IGL-1 solutions. However, the meta-analysis of the data did not prove a statistically significant difference: the UW, CS, HTK and IGL-1 solutions were associated with nearly equivalent outcomes.CONCLUSION Alternative solutions for UW yield the same degree of safety and effectiveness for the preservation of DDLs, but further well-designed clinical trials are warranted. | Agnes Lilla Szilágyi Péter Mátrai Péter Hegyi Eszter Tuboly Daniella Pécz András Garami Margit Solymár Erika Pétervári Márta Balaskó Gábor Veres László Czopf Bastian Wobbe Dorottya Szabó Juliane Wagner Petra Hartmann | 2018 | World Journal of Gastroenterology2018，24，16： | 5 |
| 3 | Magnetic resonance imaging of the cirrhotic liver: An update显示文摘Noninvasive imaging has become the standard for hepatocellular carcinoma(HCC) diagnosis in cirrhotic livers. In this review paper, we go over the basics of MR imaging in cirrhotic livers and describe the imaging appearance of a spectrum of hepatic nodules marking the progression from regenerative nodules to low- and high-grade dysplastic nodules, and ultimately to HCCs. We detail and illustrate the typical imaging appearancesof different types of HCC including focal, multifocal, massive, diffuse/infiltrative, and intra-hepatic metastases; with emphasis on the diagnostic value of MR in imaging these lesions. We also shed some light on liver imaging reporting and data system, and the role of different magnetic resonance imaging(MRI) contrast agents and future MRI techniques including the use of advanced MR pulse sequences and utilization of hepatocyte-specific MRI contrast agents, and how they might contribute to improving the diagnostic performance of MRI in early stage HCC diagnosis. | Agnes Watanabe Miguel Ramalho Mamdoh Al Obaidy Hye Jin Kim Fernanda G Velloni Richard C Semelka | 2015 | World Journal of Hepatology2015，7，3： | 5 |
| 4 | Autophagy is increased in cryptorchid testis resulting in abnormal spermatozoa显示文摘Autophagy is invoIved in spermatogenesis by regulating germ cell maturation.This catabolic process increases with hyperthermic conditions to prevent the accumulation of damaged organelles.Cryptorchidism is associated with impairment of germ cell maturation revealed by the presence of immature forms of sperm cells in ejaculates.The aim of the present study was to evaluate the status of autophagy in sperm cells from cryptorchid patients.Semen samples of cryptorchid patients and normozoospermic controls were analyzed by immuno cytochemistry and electron microscopy.Autophagy protei ns,autophagy-related protein 9(ATG9)and microtubule-associated protein,lA/lB-light chain 3(LC3)were localized by immunocytochemistry on the acrosome and on the equatorial segment of sperm cells.LC3 was also detected in the midpiece of cryptorchid sperm tail.Autophagy substrate p62 protein was present in the acrosome and in the postequatorial segment of sperm in control samples,but not in the cryptorchid ones.Transmission electron microscopy revealed double-membrane-limited autophagosomes in postequatorial part of spermatozoa head and midpiece in cryptorchid samples.Partly degraded mitochondria were frequently discerned in autophagic vacuoles.In conclusion,autophagy is increased in sperm cells from patients with cryptorchid history comparatively to control.Our work provides insights into the role of autophagy in the maturation and survival of human male gametes in pathological conditions.Thus,regulating autophagy could represent a potential way to improve sperm quality in cryptorchid men. | Marina G Yefimova Antoine Buschiazzo Agnes Burel Marie-Therese Lavault Celine Pimentel Guilhem Jouve Sylvie Jaillard Bernard Jegou Nicolas Bourmeyster Celia Ravel | 2019 | Asian Journal of Andrology2019，21，6： | 2 |
| 5 | Modeling discontinuous axisymmetric active optical membranes显示文摘 | Roger J Agnes G | 2003 | Journal of Spacecraft and Rockets2003，40，4： | 1 |
| 6 | Prevention of venous thromboembolism in surgical patients 显示文摘 | Agneli G | 2004 | Circulation2004，110，： | 1 |
| 7 | Prevention of venous thromboembolism in surgical patients 显示文摘 | Agneli G | 2004 | Circulation2004，110，23： | 1 |
| 8 | B-type natri- uretic peptide in type 2 diabetes mellitus: the influence of chronic renal failure and food 显示文摘 | LIU C AGN~S G CORCUFF J B | 2005 | Diabetes Care2005，28，3： | 1 |
| 9 | Measurements of rf bias effect in a dual electron cyclotron resonance-rf methane plasma using the langmuir probe method 显示文摘 | Junegie H Agnes G Christian L | 2000 | J Vac Sci Technol2000，18，2： | 1 |
| 10 | New modalities of regional and global left ventricular function analysis:state of the art显示文摘 | Fedele F J rambaio lo P M agn G | | 0，，01： | 1 |
| 11 | An Ontology-Based Approach to Personalized Situation-Aware Mobile Service Supply显示文摘 | Norbert W Agnes V Rudiger G | 2006 | GeoInformatica2006，，10： | 1 |
| 12 | The 'French paradox' and beyond:neuroprotective effects of polyphenols 显示文摘 | Sun A Y Agnes S Sun G Y | 2002 | Free Radical Biology & Medicine2002，5，8： | 1 |
| 13 | Inhibitory effects of tannin on human salivary a-amylase显示文摘 | Kandra L Gyongyi G Agnes Z | 2004 | Biochemical and Biophysical Research Communications2004，319，： | 1 |
| 14 | Identification of wax esters in latent print residues by GC-MS and their potential use as aging parameters显示文摘 | Agnes K Aline G Celine W | 2011 | J Forensic Ident2011，61，6： | 1 |
| 15 | Adaptivecontrol for payload launch vibration isolation显示文摘 | Jarosh J R Agnes G S Karahalis G G | 2001 | Proceedings of SPIE2001，4331，： | 1 |
| 16 | Cell culture propaga- tion of porcine rotavirus (reovirus-like agent) 显示文摘 | THEIL K W BOHL E H AGNES A G | 1977 | Am J Vet Res1977，38，11： | 1 |
| 17 | Chaotic responses of unbalanced rotor bearing stator systems with looseness or rubs显示文摘 | Agnes M Paul G | 1995 | Chaos Solitons and Fractals1995，5，9： | 1 |
| 18 | Clinical outcome in patients with locally advanced bladder carcinoma treated with conservative multimodality therapy显示文摘 | Luc George Franck Bladou Valérie Jeanne Bardou Gwenaelle Gravis Agnes Tallet Claude Alzieu Gérard Serment Naji Salem | 2004 | Urology2004，，3： | 1 |
| 19 | Comparison of the drought- stress responses of tolerant and sensitive wheat cultivars during grain filing:changes in flag leaf photosynthetic activity ABA levels, and gain yield显示文摘 | Adrienn G Irma T Agnes G | 2009 | Journal of Plant Growth Regulation2009，28，2： | 1 |
| 20 | Environmental Impacts of the Yangtze Three Gorges Project: An Overview of the Chinese-German Research Cooperation显示文摘The construction of the Three Gorges Dam on the Yangtze River in China has a great influence on the ecosystems involved. In order to investigate these environmental effects in the Yangtze catchment area as well as downstream of the dam, Forschungszentrum Jülich has organized a research network for the Chinese and German partners. In the research fields of (1) interaction water/ sediment/contaminants, (2) vegetation/biodiversity, (3) changing land use/erosion/mass movements, and (4) atmosphere, the partners have accumulated a great deal of expertise in handling major issues and also in developing models and recommendations for action. The following provides an overview of the research network and the research tasks. On the German side, five projects in research field (3) have been in operation since 2008. The results are reported in contributions by Ehret et al., Jaehnig and Cai, Schnbrodt et al., and Seeber et al.. Another five projects in research field (1) have started by September 2010. The research tasks undertaken in these five projects are presented below. | Günter Subklew Julia Ulrich Leander Fürst Agnes Hltkemeier | 2010 | Journal of Earth Science2010，21，6： | 1 |
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