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| 1 | Crustal structure of the northwestern sub-basin, South China Sea: Results from a wide-angle seismic experiment显示文摘The crustal structure of the northwestern sub-basin area of the South China Sea was modeled by inverting a wide-angle seismic survey line across the entire region and on both sides of its bounding continental margins. The survey line extended over 484 km. A total of 14 Ocean Bottom Seismometers (OBS) were deployed at intervals of 30 km to record air-gun array sources with a combined volume of 5160 in 3 . The crustal velocity structure of the northwestern sub-basin area was acquired through the integration of multi-channel seismic data. OBS data were processed and modeled initially using ray tracing inversion techniques. Results indicate that crustal thickness under the continental slope decreases from 21 to 11 km, crustal thickness of the northwestern sub-basin is 7.7 km, and the depth to the Moho ascends from 21 km under the upper continental slope to 11 km under the middle basin. The crust of the northwestern sub-basin is similar to that of the eastern sub-basin in its oceanic crustal structure. This structure has a thicker layer 1 (sedimentary layer) and a thinner layer 2. These characteristics are especially clear in the eastern sub-basin, which differs somewhat from typical oceanic crust. The tectonic geometry and velocity structure of the northwestern sub-basin and its margins comprise a symmetrical conjugate and indicate a pure shear mode with regard to the continental margin rifting mechanism. We did not find clear seismic signals from high velocity layers under the lower crust of the continental margin in the northern part of the northwestern sub-basin, which provides new evidence for the idea that the western part of the northern continental margin of the South China Sea constitutes nonvolcanic crust. Because the seafloor spreading period of the northwestern sub-basin was short, layer 2 might have experienced asymmetrical basalt magma flows, which may have blurred the magnetic anomaly lineations of the northwestern sub-basin. | WU ZhenLi LI JiaBiao RUAN AiGuo LOU Hai DING WeiWei NIU XiongWei LI XiBing | 2012 | Science China Earth Sciences2012，55，1： | 19 |
| 2 | Research progress and mechanism of nanomaterials-mediated in-situ remediation of cadmium-contaminated soil:A critical review显示文摘Cadmium contamination of soil is a global issue and in-situ remediation technology as a promising mitigation strategy has attracted more and more attention.Many nanomaterials have been applied for the in-situ remediation of cadmium-contaminated soil due to their excellent properties of the nano-scale size effect.In this work,recent research progress of various nanomaterials,including carbon nanomaterials,metal-based nanomaterials and nano mineral materials,in the removal of cadmium and in-situ remediation of cadmiumcontaminated soil were systematically discussed.Additional emphases were particularly laid on both laboratory and field restoration effects.Moreover,the factors which can affect the stability of cadmium,main interaction mechanisms between nanomaterials and cadmium in the soil,and potential future research direction were also provided.Therefore,it is believed that this work will ultimately contribute to the myriad of environmental cleanup advances,and further improve human health and sustainable development. | Yuenan Zhang Yujie Zhang Ozioma Udochukwu Akakuru Xiawei Xu Aiguo Wu | 2021 | Journal of Environmental Sciences2021，33，6： | 15 |
| 3 | Investigating the dynamic mechanical behaviors of polyurea through experimentation and modeling显示文摘Polyurea is widely employed as a protective coating in many fields because of its superior ability to improve the anti-blast and anti-impact capability of structures.In this study,the mechanical properties of polyurea XS-350 were investigated via systematic experimentation over a wide range of strain rates(0.001-7000 s^-1)by using an MTS,Instron VHS,and split-Hopkinson bars.The stress-strain behavior of polyurea was obtained for various strain rates,and the effects of strain rate on the primary mechanical properties were analyzed.Additionally,a modified rate-dependent constitutive model is proposed based on the nine-parameter Mooney-Rivlin model.The results show that the stress-strain curves can be divided into three distinct regions:the linear-elastic stage,the highly elastic stage,and an approximate linear region terminating in fracture.The mechanical properties of the polyurea material were found to be highly dependent on the strain rate.Furthermore,a comparison between model predictions and the experimental stress-strain curves demonstrated that the proposed model can characterize the mechanical properties of polyurea over a wide range of strain rates. | Hao Wang Ximin Deng Haijun Wu Aiguo Pi Jinzhu Li Fenglei Huang | 2019 | Defence Technology(防务技术)2019，15，6： | 13 |
| 4 | Crustal Structure across the Northwestern Margin of South China Sea:Evidence for Magma-poor Rifting from a Wide-angle Seismic Profile显示文摘我们从宽角度的地震侧面在 OBS2006 期间在华南海(SCS ) 的西北部分获得了的 484 km 的现在的结果航行。沿着一根以前获得的多信道的地震的线(SO4918 ) 跑的线穿过北边缘的大陆人斜坡,华南海的西北 Subbasin (NWSB ) , Zhongsha 山岳并且部分华南海的海洋的盆。在 13 张海洋底部测震表上记录的地震的节被用来从外壳的层识别测定阶段并且也从外壳披风边界(莫霍) 反映了阶段。用一个简单开始模型的 traveltimes 的倒置在学习区域揭示外壳的图象。速度模型证明在大陆人斜坡下面的外壳的厚度在 14 和 23 km 之间。线的大陆人部分被描绘由轻轻向陆地披风高举并且突然的 oceanward。在更低的外壳中的速度不超过 6.9 km/s。与新数据,我们能在线的地点排除高速度的更低的外壳的身体(在 7.0 km/s 上面的速度) 。我们断定华南海边缘的这部分由岩浆穷人 rifting 发展了。两个都, NWSB 和西南亚盆(SWSB ) 与在 5 和 7 km 之间的外壳的厚度揭示为海洋的外壳典型的速度。两者之间的 Zhongsha 山岳极其被拉长, 610 仅仅 km 大陆人外壳离开了。外壳的速度在 6.5 km/s 下面;可能显示更低的外壳的缺席。多信道的地震侧面证明 Yitongansha 在斜坡区域和 Zhongsha 山岳高举仅仅温和地被使变形。当僵硬大陆堵住哪个充当了随后导致西北亚盆的形成的主要裂缝的裂缝肩膀,我们考虑了他们。延期主要被提供由一可锻降低外壳的流动,它可能极其被稀释了并且在传播阶段期间流进海洋的盆。我们沿着北边缘比较了外壳的结构并且发现了东方西方变厚在大陆斜坡下面的外壳的趋势。这可能被沿着大陆人边缘传播的东方西方海地板贡献。 | DING Weiwei Michael SCHNABEL Dieter FRANKE RUAN Aiguo WU Zhenli | 2012 | Acta Geologica Sinica(English Edition)2012，86，4： | 12 |
| 5 | Cenozoic tectono-sedimentary characteristics and extension model of the Northwest Sub-basin,South China Sea显示文摘Based on the interpretations of three seismic profiles and one wide-angle seismic profile across the Northwest Sub-basin,South China Sea.stratigraphic sequences,deformation characteristics and an extension model for this sub-basin have been worked out.Three tectonic-stratigraphic units are determined.Detailed analyses of extension show that the event occurred mainly during the Paleogene and resulted in the formation of half-grabens or grabens distributed symmetrically around the spreading center.Sediments are characterized by chaotic and discontinuous reflectors,indicating clastic sediments. Farther to the southwest,the sub-basin features mainly continental rifting instead of sea-floor spreading. The rifting would have been controlled by the shape of the massif and developed just along the northern edge of the Zhongsha-Xisha Block,rather than joined the Xisha Trough.After 25 Ma.a southward ridge jump triggered the opening of the Southwest Sub-basin.The NW-directed stress caused by the sea-floor spreading of the Northwest Sub-basin may have prevented the continuous opening of the sub-basin.After that the Northwest Sub-basin experienced thermal cooling and exhibited broad subsidence.The deep crustal structure shown by the velocity model from a wide-angle seismic profile is also symmetrical around the spreading center,which indicates that the Northwest Sub-basin might have opened in a pure shear model. | Weiwei Ding Mingbi Li Lihong Zhao Aiguo Ruan Zhenli Wu | 2011 | Geoscience Frontiers2011，2，4： | 9 |
| 6 | A novel surface molecularly imprinted polymer as the solid-phase extraction adsorbent for the selective determination of ampicillin sodium in milk and blood samples显示文摘Surface molecularly imprinted polymers(SMIPs) for selective adsorption of ampicillin sodium were synthesized using surface molecular imprinting technique with silica gel as a support.The physical and morphological characteristics of the polymers were investigated by scanning electron microscope(SEM),Fourier transform infrared spectroscopy(FT-IR),thermogravimetric analysis(TGA),elemental analysis and nitrogen adsorption–desorption test.The obtained results showed that the SMIPs displayed great adsorption capacity(13.5 μg/mg),high recognition ability(the imprinted factor is 3.2) and good binding kinetics for ampicillin sodium.Finally,as solid phase extraction adsorbents,the SMIPs coupled with HPLC method were validated and applied for the enrichment,purification and determination of ampicillin sodium in real milk and blood samples.The averages of spiked accuracy ranged from 92.1% to 107.6%.The relative standard deviations of intra- and inter-day precisions were less than 4.6%.This study provides a new and promising method for enriching,extracting and determining ampicillin sodium in complex biological samples. | Ningli Wu Zhimin Luo Yanhui Ge Pengqi Guo Kangli Du Weili Tang Wei Du Aiguo Zeng Chun Chang Qiang Fu | 2016 | Journal of Pharmaceutical Analysis2016，6，3： | 6 |
| 7 | A study on the genetic relations between Permian Longtan Formation coal series strata and Carlin-type gold deposits,southwestern Guizhou Province,China显示文摘A large number of the Carlin-type gold deposits occur in the Longtan Formation in southwestern Guizhou Province.The Long-tan Formation contains abundant basalt,tuff and siliceous rocks.All rocks of the Longtan Formation are enriched in gold,which were deposited in a limited platform environment in the transition zone from marine to continental.The process of sedimentation was accompanied by the eruption of Emeishan basalt and hydrothermal deposition controlled by co-sedimentary submarine deep faults in the west,which led to the formation of a peculiar gold-bearing formation with coal series strata.This formation controlled the occurrence of the Carlin-type gold deposits in southwestern Guizhou Province.In response to the remobilization of the Emei mantle plume during the Yanshanian period,As,Au and other ore-forming materials were continuously extracted by deeply circulating waters from the Emeishan basalt and coal seams,thereafter forming ore-forming hydrothermal solutions.When these elements were transported in the coal seams,large amounts of As,Au and other elements were enriched in pyrite within the coal seams,thus forming high-As coal and Carlin-type gold deposits in the Longtan Formation coal series strata. | NIE Aiguo MEI Shiquan GUAN Daiyun WU Pan ZHANG Zhuru | 2008 | Chinese Journal Of Geochemistry2008，27，3： | 5 |
| 8 | PCN-Fe(Ⅲ)-PTX nanoparticles for MRI guided high efficiency chemo-photodynamic therapy in pancreatic cancer through alleviating tumor hypoxia显示文摘As nanomedicine-based clinical strategies have continued to develop,the possibility of combining chemotherapy and singlet oxygen-dependent photodynamic therapy(PDT)to treat pancreatic cancer(PaC)has emerged as a viable therapeutic modality.The efficacy of such an approach,however,is likely to be constrained by the mechanisms of drug release and tumor oxygen levels.In the present study,we developed an Fe(Ⅲ)-complexed porous coordination network(PCN)which we then used to encapsulate PTX(PCN-Fe(Ⅲ)-PTX)nanoparticles(NPs)in order to treat PaC via a combination of chemotherapy and PDT.The resultant NPs were able to release drug in response to both laser irradiation and pH changes to promote drug accumulation within tumors.Furthermore,through a Fe(Ⅲ)-based Fenton-like reaction these NPs were able to convert H2O2 in the tumor site to O2,thereby regulating local hypoxic conditions and enhancing the efficacy of PDT approaches.Also these NPs were suitable for use as a T1-MRI weighted contrast agent,making them viable for monitoring therapeutic efficacy upon treatment.Our results in both cell line and animal models of PaC suggest that these NPs represent an ideal agent for mediating effective MRI-guided chemotherapy-PDT,giving them great promise for the clinical treatment of PaC. | Tao Zhang Zhenqi Jiang Libin Chen Chunshu Pan Shan Sun Chuang Liu Zihou Li Wenzhi Ren Aiguo Wu Pintong Huang | 2020 | Nano Research2020，13，1： | 4 |
| 9 | IMPROVED ROBUST H-INFINITY ESTIMATION FOR UNCERTAIN CONTINUOUS-TIME SYSTEMS显示文摘完整顺序的柔韧的评估者的设计为连续时间的 polytopic 被调查不明确的系统。主要目的是获得一个稳定的线性评估者以便评价错误系统与规定 H ∞变细水平仍然保持要用体力地稳定。第一,一个简单其他的证明为性能最近建议了的 H ∞的一个改进 LMI 代表被给。基于阻止 Lyapunov 矩阵系统的产品的表演标准动态矩阵,为柔韧的评估者的存在的一个足够的条件以线性矩阵不平等被提供。建议设计策略允许参数依赖者 Lyapunov 功能的使用,这被显示出;因此它不比更早的结果保守。一个数字例子被采用说明可行性;建议设计的优点。 | Aiguo WU Huafeng DONG . Guangren DUAN | 2007 | Journal of Systems Science & Complexity2007，20，3： | 4 |
| 10 | Serum macrophage migration inhibitory factor as a potential biomarker to evaluate therapeutic response in patients with allergic asthma:an exploratory study显示文摘Background:Previous studies have shown that macrophage migration inhibitory factor(MIF)is involved in the pathogenesis of asthma.This study aimed to investigate whether serum MIF reflects a therapeutic response in allergic asthma.Methods:We enrolled 30 asthmatic patients with mild-to-moderate exacerbations and 20 healthy controls,analyzing the parameter levels of serum MIF,serum total immunoglobulin E(tIgE),peripheral blood eosinophil percentage(EOS%),and fractional exhaled nitric oxide(FeNO).Lung function indices were used to identify disease severity and therapeutic response.Results:Our study showed that all measured parameters in patients were at higher levels than those of controls.After one week of treatment,most parameter levels decreased significantly except for serum tIgE.Furthermore,we found that serum MIF positively correlated with EOS%as well as FeNO,but negatively correlated with lung function indices.Receiver operator characteristic(ROC)curve analysis indicated that among the parameters,serum MIF exhibited a higher capacity to evaluate therapeutic response.The area under the curve(AUC)of MIF was 0.931,with a sensitivity of 0.967 and a specificity of 0.800.Conclusions:Our results suggested that serum MIF may serve as a potential biomarker for evaluating therapeutic response in allergic asthma with mild-to-moderate exacerbations. | Huiyuan ZHU Shaochun YAN Jingshuo WU Zhong ZHANG Xiaolin LI Zheng LIU Xing MA Lina ZHOU Lin ZHANG Mingming FENG Yiwei GENG Aixin ZHANG Sabina JANCIAUSKIENE Aiguo XU | 2021 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2021，22，6： | 3 |
| 11 | Neuropeptide Y receptors:a promising target for cancer imaging and therapy 5th China-Europe Symposium on Biomaterials in Regenerative Medicine(CESB 2015)Hangzhou,China April 7–10,2015显示文摘Neuropeptide Y(NPY)was first identified from porcine brain in 1982,and plays its biological functions in humans through NPY receptors(Y1,Y2,Y4 and Y5).NPY receptors are known to mediate various physiological functions and involve in a majority of human diseases,such as obesity,hypertension,epilepsy and metabolic disorders.Recently,NPY receptors have been found to be overexpressed in many cancers,so they emerged as promising target in cancer diagnosis and therapy.This review focuses on the latest research about NPY and NPY receptors,and summarizes the current knowledge on NPY receptors expression in cancers,selective ligands for NPY receptors and their application in cancer imaging and therapy. | Juan Li Yuchen Tian Aiguo Wu | 2015 | Regenerative Biomaterials2015，2，3： | 3 |
| 12 | Observation of a bi-critical point between antiferromagnetic and superconducting phases in pressurized single crystal Ca0.73La0.27FeAs2显示文摘One of the most strikingly universal features of the high-temperature superconductors is that the superconducting phase emerges in the close proximity of the antiferromagnetic phase, and the interplay between these two phases poses a long-standing challenge. It is commonly believed that, as the antiferromagnetic transition temperature is continuously suppressed to zero, there appears a quantum critical point, around which the existence of antiferromagnetic fluctuation is responsible for the development of the superconductivity. In contrast to this scenario, we report the observation of a bi-critical point identified at 2.88 GPa and 26.02 K in the pressurized high-quality single crystal Ca_(0.73)La_(0.27)FeAs_2 by complementary in-situ high pressure measurements. At the critical pressure, we find that the antiferromagnetism suddenly disappears and superconductivity simultaneously emerges at almost the same temperature, and that the external magnetic field suppresses the superconducting transition temperature but hardly affects the antiferromagnetic transition temperature. | Yazhou Zhou ShanJiang Qi wu Vladimir A.Sidorov Jing Guo Wei Yi Shan Zhang Zhe Wang HonghongWang shu Cai Ke Yang Sheng Jiang Aiguo Li Ni Ni Guangming Zhang Liling Sun Zhongxian Zhao | 2017 | Science Bulletin2017，62，12： | 3 |
| 13 | Robust H-infinity estimation for continuous-time polytopic uncertain systems显示文摘The design of full_order robust H_infinity estimators is investigated for continuous_time polytopic uncertain systems. The main purpose is to obtain a stable and proper linear estimator such that the estimation error system remains robustly stable with a prescribed H_infinity attenuation level. Based on a recently proposed H_infinity performance criterion which exhibits a kind of decoupling between the Lyapunov matrix and the system dynamic matrices, a sufficient condition for the existence of the robust estimator is provided in terms of linear matrix inequalities. It is shown that the proposed design strategy allows the use of parameter_dependent Lyapunov functions and hence it is less conservative than earlier results. A numerical example is employed to illustrate the feasibility and advantage of the proposed design. | Aiguo WU Guangren DUAN | 2005 | 控制理论与应用(英文版)2005，3，4： | 2 |
| 14 | Chemotherapeutic nanomaterials in tumor boundary delineation:Prospects for effective tumor treatment显示文摘Accurately delineating tumor boundaries is key to predicting survival rates of cancer patients and assessing response of tumor microenvironment to various therapeutic techniques such as chemotherapy and radiotherapy.This review discusses various strategies that have been deployed to accurately delineate tumor boundaries with particular emphasis on the potential of chemotherapeutic nanomaterials in tumor boundary delineation.It also compiles the types of tumors that have been successfully delineated by currently available strategies.Finally,the challenges that still abound in accurate tumor boundary delineation are presented alongside possible perspective strategies to either ameliorate or solve the problems.It is expected that the information communicated herein will form the first compendious baseline information on tumor boundary delineation with chemotherapeutic nanomaterials and provide useful insights into future possible paths to advancing current available tumor boundary delineation approaches to achieve efficacious tumor therapy. | Ozioma Udochukwu Akakuru Zhoujing Zhang M.Zubair Iqbal Chengjie Zhu Yewei Zhang Aiguo Wu | 2022 | Acta Pharmaceutica Sinica B2022，12，6： | 2 |
| 15 | The deep structure of the Duanqiao hydrothermal field at the Southwest Indian Ridge显示文摘Polymetalic sulfide is the main product of sea-floor hydrothermal venting, and has become an important sea-floor mineral resources for its rich in many kinds of precious metal elements. Since 2007, a number of investigations have been carried out by the China Ocean Mineral Resources Research and Development Association(COMRA)cruises(CCCs) along the Southwest Indian Ridge(SWIR). In 2011, the COMRA signed an exploration contract of sea-floor polymetallic sulfides of 10 000 km2 on the SWIR with the International Seabed Authority. Based on the multibeam data and shipborne gravity data obtained in 2010 by the R/V Dayang Yihao during the leg 6 of CCCs21, together with the global satellite surveys, the characteristics of gravity anomalies are analyzed in the Duanqiao hydrothermal field(37°39′S, 50°24′E). The 'subarea calibration' terrain-correcting method is employed to calculate the Bouguer gravity anomaly, and the ocean bottom seismometer(OBS) profile is used to constrain the two-dimensional gravity anomaly simulation. The absent Moho in a previous seismic model is also calculated.The results show that the crustal thickness varies between 3 and 10 km along the profile, and the maximum crustal thickness reaches up to 10 km in the Duanqiao hydrothermal field with an average of 7.5 km. It is by far the most thicker crust discovered along the SWIR. The calculated crust thickness at the Longqi hydrothermal field is approximately 3 km, 1 km less than that indicated by seismic models, possibly due to the outcome of an oceanic core complex(OCC). | SUN Chaofeng WU Zhaocai TAO Chunhui RUAN Aiguo ZHANG Guoyin GUO Zhikui HUANG Enxian | 2018 | Acta Oceanologica Sinica2018，37，3： | 2 |
| 16 | De novo Assembly of Pen Shell(Atrina pectinata) Transcriptome and Screening of Its Genic Microsatellites显示文摘The pen shell(Atrina pectinata) is a large wedge-shaped bivalve, which belongs to family Pinnidae. Due to its large and nutritious adductor muscle, it is the popular seafood with high commercial value in Asia-Pacific countries. However, limiting genomic and transcriptomic data have hampered its genetic investigations. In this study, the transcriptome of A. pectinata was deeply sequenced using Illumina pair-end sequencing technology. After assembling, a total of 127263 unigenes were obtained. Functional annotation indicated that the highest percentage of unigenes(18.60%) was annotated on GO database, followed by 18.44% on PFAM database and 17.04% on NR database. There were 270 biological pathways matched with those in KEGG database. Furthermore, a total of 23452 potential simple sequence repeats(SSRs) were identified, of them the most abundant type was mono-nucleotide repeats(12902, 55.01%), which was followed by di-nucleotide(8132, 34.68%), tri-nucleotide(2010, 8.57%), tetra-nucleotide(401, 1.71%), and penta-nucleotide(7, 0.03%) repeats. Sixty SSRs were selected for validating and developing genic SSR markers, of them 23 showed polymorphism in a cultured population with the average observed and expected heterozygosities of 0.412 and 0.579, respectively. In this study, we established the first comprehensive transcript dataset of A. pectinata genes. Our results demonstrated that RNA-Seq is a fast and cost-effective method for genic SSR development in non-model species. | SUN Xiujun LI Dongming LIU Zhihong ZHOU Liqing WU Biao YANG Aiguo | 2017 | Journal of Ocean University of China2017，16，5： | 2 |
| 17 | Editorial显示文摘Founded only six years ago, the National Center for Nanoscience and Technology (NCNST) has developed rapidly withimportant achievements. We invited experts of NCNST in this field to introduce the research developments by NCNST | K. Ted Thurn Eric M. B. Brown Aiguo Wu Stefan Vogt Barry Lai et al. | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，10： | 2 |
| 18 | Synthesis and electrochemical properties of V_(2)C MXene by etching in opened/closed environments显示文摘The effect of etching environment(opened or closed)on the synthesis and electrochemical properties of V_(2)C MXene was studied.V_(2)C MXene samples were synthesized by selectively etching of V2AlC at 90℃in two different environments:opened environment(OE)in oil bath pans under atmosphere pressure and closed environment(CE)in hydrothermal reaction kettles under higher pressures.In OE,only NaF(sodium fluoride)+HCl(hydrochloric acid)etching solution can be used to synthesize highly pure V_(2)C MXene.However,in CE,both LiF(lithium fluoride)+HCl and NaF+HCl etchant can be used to prepare V_(2)C MXene.Moreover,the V_(2)C MXene samples made in CE had higher purity and better-layered structure than those made in OE.Although the purity of V_(2)C obtained by LiF+HCl is lower than that of V_(2)C obtained using NaF+HCl,it shows better electrochemical performance as anodes of lithium-ion batteries(LIBs).Therefore,etching in CE is a better method for preparing highly pure V_(2)C MXene,which provides a reference for expanding the synthesis methods of V_(2)C with better electrochemical properties. | Meng WU Yan HE Libo WANG Qixun XIA Aiguo ZHOU | 2020 | Journal of Advanced Ceramics2020，9，6： | 2 |
| 19 | Removal of copper(II) ions by a biosorbent— Cinnamomum camphora leaves powder显示文摘 | Hao Chen Guoliang Dai Jie Zhao Aiguo Zhong Junyong Wu Hua Yan | 2009 | Journal of Hazardous Materials2009，，1： | 2 |
| 20 | Isolation and Characterization of Melanin Pigment from Yesso Scallop Patinopecten yessoensis显示文摘Melanin is one of the essential compounds in the pigments of molluscan shells.However,the effects of melanin on color variations in molluscs are largely unknown.Our previous study suggests that Yesso scallop Patinopecten yessoensis might contain melanin pigment in the dark brown shell.We therefore isolated melanin from the pigmented shells using hydrochloric acid method,and characterized the types of melanin pigments by spectrophotometry.The purified melanin,which was verified by spectrophotometry scanning and HPLC analysis,showed the typical characteristics of melanin absorption spectra and HPLC chromatograms.The contents of pheomelanin and eumelanin in pigmented shells,which were determined by the linear standard curve of melanin at 405 nm and 350 nm absorbance,were 48.23 ? 1.350 and 157.65 ? 5.905 mg,respectively.The present results indicate that the brown-pigmented shells of scallops comprise approximately 76.6% of eumelanin and 23.4% of pheomelanin,which supports the presence of eumelanin-rich pigment in scallop shells.Therefore,the combination of hydrochloric acid extraction and spectrophotometric quantification is a rapid and efficient method to isolate and quantify melanin in shells.This will facilitate the melanin studies related to shell color polymorphism and the selective breeding of bivalves with different shell colors. | SUN Xiujun WU Biao ZHOU Liqing LIU Zhihong DONG Yinghui YANG Aiguo | 2017 | Journal of Ocean University of China2017，16，2： | 2 |
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