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| 1 | A 3D-printed modular magnetic digital microfluidic architecture for on-demand bioanalysis显示文摘Magnetic digital microfluidics(MDM)manipulates fluids in the form of droplets on an open substrate,and incorporates surface energy traps(SETs)to facilitate the droplet manipulation.Conventional MDM devices are fabricated monolithically,which makes it difficult to modify the device configuration without completely overhauling the original design.In this paper,we present a modular MDM architecture that enables rapid on-demand configuration and re-configuration of MDM platforms for customized bioanalyses.Each modular component contains a SET and a Lego-like antistud that fits onto a base board with Lego-like studs.We illustrate the versatility of the modular MDM architecture in biomarker sensing,pathogen identification,antibiotic resistance determination,and biochemical quantification by demonstrating immunoassays,phenotypical assays and enzymatic assays on various modular MDM platforms configured on demand to accomplish the fluidic operations required by assorted bioanalytical assays.The modular MDM architecture promises great potential for point-of-care diagnostics by offering on-demand customization of testing platforms for various categories of diagnostic assays.It also provides a new avenue for microfluidic assay development with its high configurability which would significantly reduce the time and cost of the development cycle. | Pojchanun Kanitthamniyom Aiwu Zhou Shilun Feng Aiqun Liu Shawn Vasoo Yi Zhang | 2020 | Microsystems & Nanoengineering2020，6，1： | 3 |
| 2 | A 3D-printed magnetic digital microfluidic diagnostic platform for rapid colorimetric sensing of carbapenemase-producing Enterobacteriaceae显示文摘Carbapenemase-producing Enterobacteriaceae(CPE)are a group of drug-resistant Gram-negative pathogens that are classified as a critical threat by the World Health Organization(WHO).Conventional methods of detecting antibiotic-resistant pathogens do not assess the resistance mechanism and are often time-consuming and laborious.We have developed a magnetic digital microfluidic(MDM)platform,known as MDM Carba,for the identification of CPE by measuring their ability to hydrolyze carbapenem antibiotics.MDM Carba offers the ability to rapidly test CPE and reduce the amount of reagents used compared with conventional phenotypic testing.On the MDM Carba platform,tests are performed in droplets that function as reaction chambers,and fluidic operations are accomplished by manipulating these droplets with magnetic force.The simple droplet-based magnetic fluidic operation allows easy system automation and simplified hands-on operation.Because of the unique“power-free”operation of MDM technology,the MDM Carba platform can also be operated manually,showing great potential for point-of-care testing in resource-limited settings.We tested 27 bacterial isolates on the MDM Carba platform,and the results showed sensitivity and specificity that were comparable to those of the widely used Carba NP test.MDM Carba may shorten the overall turnaround time for CPE identification,thereby enabling more timely clinical decisions for better clinical outcomes.MDM Carba is a technological platform that can be further developed to improve diagnostics for other types of antibiotic resistance with minor modifications. | Pojchanun Kanitthamniyom Pei Yun Hon Aiwu Zhou Mohammad Yazid Abdad Zhi Yun Leow Nurhidayah Binte Mohamed Yazid Vanessa Lim Wei Xun Shawn Vasoo Yi Zhang | 2021 | Microsystems & Nanoengineering2021，7，3： | 0 |
| 3 | Effects of Different Feeding Patterns on the Growth of Micropterus salmoides显示文摘Three experimental groups with different feeding frequencies,different feeding rates and compensatory feeding were set up to study their effects on the growth of Micropterus salmoides.The results showed that different feeding rates of 2%,3%and 4%had no significant effects on the growth of M.salmoides.Therefore,before the individual weight of 50 g,the fish could be fed with a feeding rate of 4%,which could be gradually decreased to 2%in the later period.Under the condition of relatively fixed feeding rate,different feeding frequencies had an impact on the growth of M.salmoides.Under the conditions of suitable water temperature,it is reasonable to feed twice daily.The experimental fish fed for 5 d and then deprived of feed for 1 d showed fully compensatory growth,and there were no significant differences in weight gain rate,daily weight gain and specific growth rate compared with the continuous feeding group(P>0.05).Therefore,according to the feeding conditions of fish in the breeding process,stopping feeding for 1 d every 5 d could save feed and labor and improve breeding efficiency. | Yuzhang HAN Zhourui WEN Aiwu YI Jun HUANG Zhiqiang ZHU Wenyu TONG Hongping ZHENG | 2023 | Agricultural Biotechnology2023，12，2： | 0 |
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