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| 1 | Increased catabolism and decreased unsaturation of ganglioside in patients with inflammatory bowel disease显示文摘AIM: To investigate whether accelerated catabolism of ganglioside and decreased ganglioside content contribute to the etiology of pro-inflammatory intestinal disease. METHODS: Intestinal mucosa from terminal ileum or colon was obtained from patients with ulcerative colitis or inflammatory Crohn's disease(n = 11) undergoing bowel resection and compared to control samples of normal intestine from patients with benign colon polyps(n = 6) and colorectal cancer(n = 12) in this observational case-control study. Gangliosides and phospholipids of intestinal mucosa were characterized by class and ceramide or fatty acid composition using liquid chromatography triple-quad mass spectrometry. Content and composition of ganglioside classes GM1, GM3, GD3, GD1 a, GT1 and GT3 were compared among subject groups. Content and composition of phospholipid classes phosphatidylcholine(PC) and phosphatidylethanolamine were compared among subject groups. Unsaturation index of individual ganglioside and phospholipid classes was computed and compared among subject groups. Ganglioside catabolism enzymes beta-hexosaminidase A(HEXA) and sialidase-3(NEU3) were measured in intestinal mucosa using western blot and compared among subject groups. RESULTS: Relative GM3 ganglioside content was 2-fold higher(P < 0.05) in intestine from patients with inflammatory bowel disease(IBD) compared to control intestine. The quantity of GM3 and ratio of GM3/GD3 was also higher in IBD intestine than control tissue(P < 0.05). Control intestine exhibited 3-fold higher(P < 0.01) relative GD1 a ganglioside content than IBD intestine. GD3 and GD1 a species of ganglioside containing three unsaturated bonds were present in control intestine, but were not detected in IBD intestine. The relative content of PC containing more than two unsaturated bonds was 30% lower in IBD intestine than control intestine(P < 0.05). The relative content of HEXA in IBD intestine was increased 1.7-fold(P < 0.05) and NEU3 was increased 8.3-fold(P < 0.01) compared to normal intestine. Intestinal mucosa in IBD is characterized by increased GM3 content, decreased GD1 a, and a reduction in polyunsaturated fatty acid constituents in GD3, GD1 a and PC.CONCLUSION: This study suggests a new paradigm by proposing that IBD occurs as a consequence of increased metabolism of specific gangliosides. | John J Miklavcic Glen K Shoemaker Vera C Mazurak M Tom Clandinin Tasha DL Hart Kareena L Schnabl Gordon M Lees Bodil MK Larsen Oliver F Bathe Alan BR Thomson M Tom Clandinin | 2015 | World Journal of Gastroenterology2015，21，35： | 3 |
| 2 | Distinct biological roles for the notch ligands Jagged-1 and Jagged-2显示文摘 | Choi K Alan YH Gibbons DL | 2009 | J Biol Chem2009，284，17： | 1 |
| 3 | Regional patterns of disability-free life expectancy and disability adjusted life expectancy: Global Burden of Disease Study 显示文摘 | Murray CJL Alan DL | 1997 | The lancet1997，349，： | 1 |
| 4 | Transforming growth factor betal Cancer显示文摘 | Alan DL Harinder SG | | 1990，85，3： | 1 |
| 5 | Inhibition of cholestery ester deposition in macrophages by calcium entry blockers:an effect dissociable from calcium entry blockade 显示文摘 | Rateri DL Schonfeld G | 1987 | Br J Pharmaclo1987，91，： | 1 |
| 6 | Inhibition of cholestery es- ter deposition in macrophages by calcium entry blockers: An effect dissociable from calcium entry blockade显示文摘 | Alan D Rateri DL Sehonfeld G | 1987 | Br J Pharma- clo1987，91，： | 1 |
| 7 | lnhibition of cholesteryes terde positionin macrophages by calcium entry blockers: an effect dissociable from calcium entry blockade 显示文摘 | Alan D Rateri DL Schonfeld G | 1987 | Br J Pharmacol1987，91，： | 1 |
| 8 | Inhibition of cholestery ester deposition in macrophages by calcium entry blockers: an effect dissociable from calcium entry blockade显示文摘 | Alan D Rateri DL Schonfeld G | 1987 | Br J Pharmacol1987，91，： | 1 |
| 9 | The effects of connexin phosphorylation on gap junctional communication显示文摘 | Paul DL Alan FL | 2004 | Int J Biochem Cell Biol2004，36，7： | 1 |
| 10 | Inhibition of cholestery ester deposition in macrophages by calcium entry blockers: an effect dissociable from calcium entry blockade显示文摘 | Alan D Rateri DL Schonfeld G | 1987 | Br J Pharmaclo1987，91，： | 1 |
| 11 | Primary pneumothorax:Should surgery be offered after the first episode?显示文摘Surgery is the recommended and most effective means of preventing the recurrence of primary spontaneous pneumothorax(PSP). However, the conventional belief amongst most clinicians is that surgery should not be routinely offered to patients with an uncomplicated first episode of PSP. The view that surgery should be reserved for recurrent episodes of ipsilateral PSP is based on an apprehension regarding traumatic thoracicsurgery combined with a perception that recurrences after a single episode of PSP are unlikely. Modern advances in minimally invasive thoracic surgery have now dramatically reduced the morbidity of PSP surgery. Such surgery is now safe, effective and causes minimal indisposition for patients. On the other hand, modern clinical data suggests that recurrence rate of PSP is perhaps much higher than previously assumed, with more than half of patients experiencing a second episode within several years of the first. With such new appreciations of the current situation, it is appropriate to now consider offering surgery to patients even after the first episode of PSP. | Alan DL Sihoe Peter SY Yu Jerry WL Yeung | 2015 | World Journal of Respirology2015，5，1： | 0 |
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