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| 1 | Helicobacter pylori and EBV in gastric carcinomas:Methylation status and microsatellite instability显示文摘AIM:To verify the methylation status of CDH1, DAPK, COX2, hMLH1 and CDKN2A genes and to evaluate their association with Helicobacter pylori (H. pylori)-cagA+ and Epstein Barr virus (EBV) infections in gastric adenocarcinomas.METHODS: Methylation-specific PCR (MSP) assay was performed in 89 primary gastric carcinomas (intestinal and diffuse types). Microsatellite instability (MSI) analysis was performed using the BAT26 primer set and PCR products were analyzed with the ABI PRISM 3100 Genetic Analyzer using Genescan 3.7 software (Applied Biosystems). Detection of H. pylori and genotyping were performed by PCR, using specifi c primers for ureaseC and cagA genes. The presence of EBV was assessed by in situ hybridization. Statistical analyses were performed using the χ2 or Fisher's exact test.RESULTS: The most frequent hypermethylated gene was COX-2 (63.5%) followed by DAPK (55.7%), CDH1 (51%), CDKN2A (36%) and hMLH1 (30.3%). Intestinal and diffuse adenocarcinomas showed different methylation profiles and there was an association between methylation of E-CDH1 and H. pylori-cagA+ in the intestinal adenocarcinoma type. MSI was correlated with hMLH1 methylation. There was an inverse correlation between DAPK hypermethylation and MSI.CONCLUSION: We found a strong association between CDH1 methylation and H. pylori-cagA+ in intestinal-type gastric cancer, association of MSI and better prognosis and an heterogeneous COX-2 overexpression. | Adriana Camargo Ferrasi Nídia Alice Pinheiro Silvia Helena Barem Rabenhorst Otávia Luisa Caballero Maria Aparecida Marchesan Rodrigues Fabrício de Carvalho Celso Vieira de Souza Leite Marcia Valéria Pitombeira Ferreira Marcos Aurélio Pessoa Barros Maria Inês de Moura Campos Pardini | 2010 | World Journal of Gastroenterology2010，16，3： | 6 |
| 2 | Th1 cytokines promote T-cell binding to antigen-presenting cells via enhanced hyaluronan production and accumulation at the immune synapse显示文摘Hyaluronan(HA)production by dendritic cells(DCs)is known to promote antigen presentation and to augment T-cell activation and proliferation.We hypothesized that pericellular HA can function as intercellular‘glue’directly mediating T cell–DC binding.Using primary human cells,we observed HA-dependent binding between T cells and DCs,which was abrogated upon pre-treatment of the DCs with 4-methylumbelliferone(4-MU),an agent which blocks HA synthesis.Furthermore,T cells regulate HA production by DCs via T cell-derived cytokines in a T helper(Th)subset-specific manner,as demonstrated by the observation that cell-culture supernatants from Th1 but not Th2 clones promote HA production.Similar effects were seen upon the addition of exogenous Th1 cytokines,IL-2,interferon c(IFN-c)and tumor necrosis factor a(TNF-a).The critical factors which determined the extent of DC–T cell binding in this system were the nature of the pre-treatment the DCs received and their capacity to synthesize HA,as T-cell clones which were pre-treated with monensin,added to block cytokine secretion,bound equivalently irrespective of their Th subset.These data support the existence of a feedforward loop wherein T-cell cytokines influence DC production of HA,which in turn affects the extent of DC–T cell binding.We also document the presence of focal deposits of HA at the immune synapse between T-cells and APC and on dendritic processes thought to be important in antigen presentation.These data point to a pivotal role for HA in DC–T cell interactions at the IS. | Paul L Bollyky Stephen P Evanko Rebecca P Wu Susan Potter-Perigo S Alice Long Brian Kinsella Helena Reijonen Kelly Guebtner Brandon Teng Christina K Chan Kathy R Braun John A Gebe Gerald T Nepom Thomas N Wight | 2010 | Cellular & Molecular Immunology2010，7，3： | 3 |
| 3 | Management of Evans syndrome显示文摘 | Alice N Irene R | 2006 | Br J Haematol2006，132，2： | 1 |
| 4 | The effect of cues on gait vari- ability-reducing the attentional cost of walking in people with Parkinson's disease 显示文摘 | Katherine B Lynn R Alice N | 2008 | Parkinsonism Relat Disord2008，14，4： | 1 |
| 5 | Coercive strategy in interfirm cooperation : Mediating roles of interpersonal and interorgani- zational trust 显示文摘 | STEVENS L HANG -YUE N ALICE H Y H | 2006 | Journal of Business Research2006，59，： | 1 |
| 6 | Recreation carrying capacity estimations to support beach management at Praia de Faro, Portugal 显示文摘 | DANIEL A Z ALLAN T W ALICE N | 2011 | Applied Geography2011，31，3： | 1 |
| 7 | Image restoration usingwavelet based image fusion 显示文摘 | Anni U G Alice N C Mangesh D N | 2011 | International Journal cff Engi- neering Science and Technology2011，3，2： | 1 |
| 8 | The Effects of the Question' What Do You Want?' on Mand Training Outcomes of Children with Autism 显示文摘 | Bowen Crystal N Shillingsburg M Alice Carr James E | 2012 | Journal of Applied Behavior Analysis2012，，45： | 1 |
| 9 | Activation of autoimmunity following use of immunostimulatory herbal supplements显示文摘 | Alice N L Victoria P W | 2004 | Arch Dermatol2004，140，： | 1 |
| 10 | Un- calibrated stroke volume variations are able to predict the hemodynamic effects of positive end-expiratory pressure in pa- tients with acute lung injury or acute res- piratory distress syndrome after liver transplantation 显示文摘 | Mattieu B Karine N Alice Q | 2009 | Anesthesiology2009，111，： | 1 |
| 11 | Nitrate and nitrite quantification from cured meat and vegetables and their estimated dietary in- take in Australians显示文摘 | Hsu J Arcot J Alice lee N | 2009 | Food Chemistry2009，115，1： | 1 |
| 12 | New perspectives in diet analysis based on DNA barcoding and parallel pyrosequeneing: the trn- Lapproach 显示文摘 | I Alice V Christian M Muhammad A N | 2009 | Molecular Ecology Resources2009，9，2009： | 1 |
| 13 | Rate and extent of digestion of the ethanol-soluble and neutral detergent-insoluble fraction of corn grain 显示文摘 | Chen Y K Alice N Larry P | 1999 | J Anita Sci1999，77，： | 1 |
| 14 | A new label technology for the detection of specific polymerase chain reaction production in a closed tube显示文摘 | ALICE Y FERO S | 2000 | Nucleic Acids Res2000，1528，8： | 1 |
| 15 | Nitrate and nitrite quantification from cured meat and vegetables and their estimated dietary intake in Australians 显示文摘 | James Hsu Jayashree Arcot N Alice Lee | 2009 | Food Chemistry2009，115，： | 1 |
| 16 | Measurement and kinetic analysis of the neutral detergent-soluble carbohydrate fraction of legumes and grasses显示文摘 | Schofield P Alice N P | 1995 | J Anita Sci1995，73，： | 1 |
| 17 | Developmentof a solid-phaseextraction-enzyme-linked immunosorbent assay method for the determination of estrone in water显示文摘 | Li Zhilan Wang Shuo Alice Lee N | 2004 | Analytica Chimica Acta2004，503，： | 1 |
| 18 | Consumer Psychology显示文摘 | ALICE M T ARTZ N | 1994 | Annual Review of Psychology1994，45，6： | 1 |
| 19 | Selectivity and proofreading both contribute significantly to the fidelity of RNA polymerase Ⅲ transcription 显示文摘 | Alic N Ayoub N Landrieux E | 2007 | Proc Natl Acad Sci U S A2007，104，10： | 1 |
| 20 | Does freezing in Parkin- son's'disease change limb coordination 显示文摘 | Alice N Fabienne C Anne-Marie W | 2007 | J Neurol2007，254，9： | 1 |
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