| 1 | Cross-species single-cell transcriptomic analysis reveals divergence of cell composition and functions in mammalian ileum epithelium显示文摘Animal models are widely used for biomedical studies and drug evaluation.The small intestine plays key roles in nutrient absorption,hormone secretion,microbiota defense and drug absorption and metabolism.Although the intestinal structure of mammals is conserved,the differences on epithelial cell composition,functional assignments and drug absorption among mammals are largely unknown.Here,cross-species analysis of single-cell transcriptomic atlas of the ileum epithelium from mouse,rat,pig,macaque and human reveals the conserved and differential cell types and functions among species,identifies a new CA7+cell type in pig,macaque and human ileum,uncovers the distinct expression pattern in enterocytes,enteroendocrine cells and Paneth cells,and defines the conserved and species-specific intestinal stem cell signature genes.The examination of drug absorption across species suggests that drug metabolism in mouse ileum is closer to human while drug transport in macaque ileum is more similar to human.Together,our data provide the comprehensive information about cell composition and functional assignments in five species,and offer the valuable guidance for animal model selection and drug testing. | Haonan Li Xiaodan Wang Yalong Wang Mengxian Zhang Fan Hong Hong Wang Along Cui Jianguo Zhao Weizhi Ji Ye-Guang Chen | 2022 | Cell Regeneration2022，11，1： | 2 |
| 2 | Establishment of porcine and monkey colonic organoids for drug toxicity study显示文摘Pig and monkey are widely used models for exploration of human diseases and evaluation of drug efficiency and toxicity,but high cost limits their uses.Organoids have been shown to be promising models for drug test as they reasonably preserve tissue structure and functions.However,colonic organoids of pig and monkey are not yet established.Here,we report a culture medium to support the growth of porcine and monkey colonic organoids.Wnt signaling and PGE2 are important for long-term expansion of the organoids,and their withdrawal results in lineage differentiation to mature cells.Furthermore,we observe that porcine colonic organoids are closer to human colonic organoids in terms of drug toxicity response.Successful establishment of porcine and monkey colonic organoids would facilitate the mechanistic investigation of the homeostatic regulation of the intestine of these animals and is useful for drug development and toxicity studies. | Haonan Li Yalong Wang Mengxian Zhang Hong Wang Along Cui Jianguo Zhao Weizhi Ji Ye-Guang Chen | 2021 | Cell Regeneration2021，10，1： | 1 |
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