|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Three-dimensional bioprinted glioblastoma microenvironments model cellular dependencies and immune interactions显示文摘Brain tumors are dynamic complex ecosystems with multiple cell types.To model the brain tumor microenvironment in a reproducible and scalable system,we developed a rapid three-dimensional(3D)bioprinting method to construct clinically relevant biomimetic tissue models.In recurrent glioblastoma,macrophages/microglia prominently contribute to the tumor mass.To parse the function of macrophages in 3D,we compared the growth of glioblastoma stem cells(GSCs)alone or with astrocytes and neural precursor cells in a hyaluronic acid-rich hydrogel,with or without macrophage.Bioprinted constructs integrating macrophage recapitulate patient-derived transcriptional profiles predictive of patient survival,maintenance of stemness,invasion,and drug resistance.Whole-genome CRISPR screening with bioprinted complex systems identified unique molecular dependencies in GSCs,relative to sphere culture.Multicellular bioprinted models serve as a scalable and physiologic platform to interrogate drug sensitivity,cellular crosstalk,invasion,context-specific functional dependencies,as well as immunologic interactions in a species-matched neural environment. | Min Tang Qi Xie Ryan C.Gimple Zheng Zhong Trevor Tam Jing Tian Reilly L.Kidwell Qiulian Wu Briana C.Prager Zhixin Qiu Aaron Yu Zhe Zhu Pinar Mesci Hui Jing Jacob Schimelman Pengrui Wang Derrick Lee Michael H.Lorenzini Deobrat Dixit Linjie Zhao Shruti B.hargava Tyler E.Miller Xueyi Wan Jing Tang Bingjie Sun Benjamin F.Cravatt Alysson R.Muotri Shaochen Chen Jeremy N.Rich | 2020 | Cell Research2020，30，10： | 6 |
| 2 | The uric acid metabolism pathway as a therapeutic target in hyperuricemia related to metabolic syndrome显示文摘 | André a Name Colado Sim? o Marcell Alysson Batisti Lozovoy Isaias Dichi | 2012 | Expert Opinion on Therapeutic Targets2012，，12： | 2 |
| 3 | Development of hepatorenal syndrome in bile duct ligated rats显示文摘AIM: To evaluate in bile duct ligated rats whether there were progressive alterations of renal function without changes in histopathology. METHODS: Male Wistar rats were submitted to sham-surgery or bile duct ligation (BDL) and divided according to the post-procedure time (2, 4 and 6-wk). To determine renal function parameters, rats wereplaced in metabolic cages and, at the end of the experiment, blood and urine samples were obtained. Histology and hydroxyproline content were analyzed in liver and renal tissue. RESULTS: Rats with 2 wk of BDL increased free water clearance (P = 0.02), reduced urinary osmolality (P = 0.03) and serum creatinine (P = 0.01) in comparison to the sham group. In contrast, rats at 6 wk of BDL showed features of HRS, including signif icant increase in serum creatinine and reductions in creatinine clearance, water excretion and urinary sodium concentration. Rats with 4 wk of BDL exhibited an intermediate stage of renal dysfunction. Progressive hepatic f ibrosis according to post-procedure time was confirmed by histology. The increased levels of liver hydroxyproline contrasted with the absence of structural changes in the kidney, as assessed by histology and unchanged hydroxyproline content in renal tissue. CONCLUSION: Our data show that BDL produced progressive renal dysfunction without structural changes in the kidney, characterizing HRS. The present model will be useful to understand the pathophysiology of HRS. | Regina M Pereira Robson AS dos Santos Eduardo A Oliveira Virgínia HR Leite Filipi LC Dias Alysson S Rezende Lincoln P Costa Lucíola S Barcelos Mauro M Teixeira Ana Cristina Sim■es e Silva | 2008 | World Journal of Gastroenterology2008，14，28： | 2 |
| 4 | A Model for Neural Development and Treatment of Rett Syndrome Using Human Induced Pluripotent Stem Cells显示文摘 | Maria C.N. Marchetto Cassiano Carromeu Allan Acab Diana Yu Gene W. Yeo Yangling Mu Gong Chen Fred H. Gage Alysson R. Muotri | 2010 | Cell2010，，4： | 1 |
| 5 | 压力支持通气和双相气道正压通气通过肺血流重新分布从而提高氧合显示文摘背景机械通气时保留自主呼吸(spontaneousbreathing,SB)可改善气体交换,主要是因为这种通气方式可使萎陷的肺复张。在保留SB的机械通气中,常选择压力支持通气(pressuresupportventilation,PSV)和双相气道正压通气(biphasicpositiveairwaypressure,BIPAP)。但医师对这些辅助性机械通气方式改善肺功能的机制却不甚了解。我们评价了采用PSV和BIPAP改善氧合的机制。方法5头猪(25-29.3kg)于仰卧位行机械通气,并通过清除肺表面活性物质诱发其发生急性肺损伤(acutelung砸jury,Au)。状态稳定后,给予BIPAP通气,初期较低持续气道正压设置为5cmH2O,随后逐渐增加持续气道正压至维持潮气量在6-8ml/kg。减浅麻醉深度,当SB≥每分通气量的20%时,随机进行1小时的PSV或BIPAP+SB通气。于呼气末行全胸螺旋式计算机体层扫描,并记录肺功能参数。静脉给予荧光微球体来记录肺血流(pulmonarybloodflow,PBF),并利用空间聚类分析来评估每种通气方式对PBF重新分布的影响。结果Au损伤肺功能并加大了下肺萎陷或不张组织的面积(P〈0.05)。和对照组比较,PSV和BIPAP+SB的通气模式提高了氧合并减少了静脉血掺杂(P〈0.05)。尽管如此,我们观察到自主呼吸时下肺的不张肺组织或通气不良组织明显增加,而可正常通气的肺组织减少。观察到6例中有5例行PSV或sB+BIPAP通气时PBF由下至上重新分布,肺通气也较好。结论在ALI模型中采用PSV或BIPAP+SB行机械通气可改善氧合,减少静脉血掺杂的原因为PBF由下至上的重新分布,而不是因为下肺复张。 | Alysson R. Carvalho, PhD Peter M. Spieth, MD Paolo Pelosi, MD, PhD Alessandro Beda, PhD Agnaldo J. Lopes, MD, DSc Boriana Neykova, MD Axel R. Heller, MD, PhD Thea Koch, MD, PhD Marcelo Gama de Abreu, MD, MSc, PhD, DEAA 王琦(翻译) | 2012 | 麻醉与镇痛2012，8，1： | 1 |
| 6 | Evaluation of Naphthenic Acidity Number and Temperature on the Cor- rosion Behavior of Stainless Steels by Using Electrochem- ical Noise Technique显示文摘 | FERNANDA H ANA C A ALYSSON N D | 2014 | Electrochimica Acta2014，124，： | 1 |
| 7 | Reduced-energy cranberry juice increases folic acid and adiponectin and reduces homocysteine and oxidative stress in patients with the metabolic syndrome显示文摘 | Tathiana Name Colado Sim?o Marcell Alysson Batisti Lozovoy Andréa Name Colado Sim?o Sayonara Rangel Oliveira Danielle Venturini Helena Kaminami Morimoto Lúcia Helena Silva Miglioranza Isaias Dichi | 2013 | British Journal of Nutrition2013，，10： | 1 |
| 8 | On Byzantine generals with alternative plans显示文摘 | Miguel Correia Alysson Neves Bessani Paulo Veríssimo | 2008 | Journal of Parallel and Distributed Computing2008，，9： | 1 |
| 9 | Spray drying conditions and encap sulating composition effects on formation and properties of sodium diclofenac microparticles显示文摘 | Alysson L R R Wanderley P O | 2006 | Powder Technology2006，171，： | 1 |
| 10 | Wnt-mediated activation of Neuro D1and retro-elements during adult neurogenesis显示文摘 | Tomoko K Jenny H Alysson M | 2009 | Nature Neurosci2009，12，： | 1 |
| 11 | A look to the old-world sky: EU- funded dependability cloud computing research 显示文摘 | Alysson B Rudiger K Data P | 2012 | ACM AIGOPS Operating Systems Review2012，46，2： | 1 |
| 12 | Beneficial Effects Of Lactobacillus Plantarum On Glycemia And Homocysteine Levels In Postmenopausal Women With Metabolic Syndrome: A Pilot Study显示文摘 | Fabíola Málaga Barreto Andréa Name Colado Sim?o Helena Kaminami Morimoto Marcell Alysson Batisti Lozovoy Isaias Dichi Lúcia Helena da Silva Miglioranza | 2013 | Nutrition2013，，： | 1 |
| 13 | Astroglial cells regulate the developmental timeline of human neurons differentiated from induced pluripotent stem cells显示文摘 | Xin Tang Li Zhou Alecia M. Wagner Maria C.N. Marchetto Alysson R. Muotri Fred H. Gage Gong Chen | 2013 | Stem Cell Research2013，，2： | 1 |
| 14 | Multipotent Stem Cells from Umbilical Cord: Cord Is Richer than Blood!显示文摘 | MarianeSecco EderZucconi Natassia M.Vieira Luciana L.Q.Foga?a AntoniaCerqueira Maria Denise F.Carvalho TatianaJazedje Oswaldo K.Okamoto Alysson R.Muotri MayanaZatz | 2009 | STEM CELLS2009，，1： | 1 |
| 15 | Gene transfer of human apoe isoforms results in differential modulation of amyloid deposi- tion and neurotoxicity in mouse brain 显示文摘 | Eloise H Jonathan D Alysson DR | 2013 | Science Translational Medicine2013，5，212： | 1 |
| 16 | Dialkyl imidazolium acetate ionosilica as efficient and recyclable organocatalyst for cyanosilylation reactions of ketones显示文摘We report new heterogeneous organocatalyst based on silica hybrid supported N-heterocyclic carbene(NHC-)species.The organocatalyst is formed from an imidazolium iodide based ionosilica material,followed by iodide/acetate anion exchange.The imidazolium acetate generates the organocatalytic carbene via partial deprotonation of the imidazolium ring in situ.As monitored via EDX,solid state NMR and ion chromatography measurements,the iodide/acetate exchange involving the imidazolium ionosilica material took place only in small extent.Despite the fact that the exchanged material contains only a very small amount of acetate,we observed good catalytic activity and recyclability in cyanosilylation reactions of ketones with trimethylsilyl cyanide.The versatility of the catalyst was highlighted via reaction with several substrates,yielding the corresponding cyanohydrins in good yields.In recycling experiments,the material showed decreasing catalytic activity starting from the third reaction cycle,but high catalytic activity can be regenerated via another acetate treatment.Our work is important as it highlights the possibility to combine carbene chemistry and silica,which are antagonistic at a first glance.We show that imidazolium acetate based ionosilicas are therefore heterogeneous'proto-carbenes',and that there is no need to form strongly basic silica supported NHCs to obtain heterogeneous NHC-organocatalysts.This work therefore opens the route towards heterogeneous and re-usable NHC-organocatalysts from supported ionic liquid imidazolium acetates. | Thanh Tran Duy Alysson Duarte Rodrigues Giang Vo-Thanh Peter Hesemann | 2020 | Green Energy & Environment2020，5，2： | 1 |
| 17 | Reduced-energy cranberry juice increases folic acid and adiponectin and reduces homocysteine and oxidative stress in patients with the metabolic syndrome显示文摘 | Tathiana Name Colado Sim?o Marcell Alysson Batisti Lozovoy Andréa Name Colado Sim?o Sayonara Rangel Oliveira Danielle Venturini Helena Kaminami Morimoto Lúcia Helena Silva Miglioranza Isaias Dichi | 2013 | British Journal of Nutrition2013，，10： | 1 |
| 18 | Spray drying conditions and encapsulating composition effects on formation and properties of sodium diclofenac micropartieles显示文摘 | Alysson L R R Wanderley P O | 2006 | Powder Technology2006，171，1： | 1 |
| 19 | ADHD and epilep-sy:Contributions from the use of behavioral rating scales to investi-gate psychiatric comorbidities显示文摘 | Karina SL Alysson MC Joel AL Jane de AN | 2011 | Epilepsy Behav2011，20，3： | 1 |
| 20 | A look to the old-world sky: EU- funded dependability cloud computing research显示文摘 | Alysson B Rudiger K Data P | 2012 | ACM AIGOPS Operating Systems Review2012，46，2： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员