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| 1 | 3D-printed gelatin methacrylate (GelMA)/ silanated silica scaffold assisted by two-stage cooling system for hard tissue regeneration显示文摘Among many biomaterials,gelatin methacrylate(GelMA),a photocurable protein,has been widely used in 3D bioprinting process owing to its excellent cellular responses,biocompatibility and biodegradability.However,GelMA still shows a low processability due to the severe temperature dependence of viscosity.To overcome this obstacle,we propose a two-stage temperature control system to effectively control the viscosity of GelMA.To optimize the process conditions,we evaluated the temperature of the cooling system(jacket and stage).Using the established system,three GelMA scaffolds were fabricated in which different concentrations(0,3 and 10 wt%)of silanated silica particles were embedded.To evaluate the performances of the prepared scaffolds suitable for hard tissue regeneration,we analyzed the physical(viscoelasticity,surface roughness,compressive modulus and wettability)and biological(human mesenchymal stem cells growth,western blotting and osteogenic differentiation)properties.Consequently,the composite scaffold with greater silica contents(10 wt%)showed enhanced physical and biological performances including mechanical strength,cell initial attachment,cell proliferation and osteogenic differentiation compared with those of the controls.Our results indicate that the GelMA/silanated silica composite scaffold can be potentially used for hard tissue regeneration. | Eunjeong Choi Dongyun Kim Donggu Kang Gi Hoon Yang Bongsu Jung MyungGu Yeo Min-Jeong Park SangHyun An KyoungHo Lee Jun Sik Kim Jong Chul Kim Woonhyeok Jeong Hye Hyun Yoo Hojun Jeon | 2021 | Regenerative Biomaterials2021，8，2： | 2 |
| 2 | Reappraisal of serum alpha‐foetoprotein as a surveillance test for hepatocellular carcinoma during entecavir treatment显示文摘 | Gi‐Ae Kim Chang Hyeon Seock Jang Won Park Jihyun An Kwang‐Sun Lee Jee Eun Yang Young‐Suk Lim Kang Mo Kim Ju Hyun Shim Danbi Lee Han Chu Lee | 2015 | Liver Int2015，，1： | 2 |
| 3 | The GUSTO investigators显示文摘 | An international randomized trial comparing four thrombolytic strate gies for acute myocardial infarction | 1993 | N Engl JM ed1993，329，10： | 1 |
| 4 | Laminar shear stress inhibits lipid perox- idation induced by high glucose plus arachidonic acid in endothelial cells显示文摘 | Mun GI An SM Park H | 2008 | Am J Physiol Heart Circ Physiol2008，295，5： | 1 |
| 5 | Vehicle dynamic analysis using virtual proving ground approach显示文摘 | Han Ki Min Gi Seob Choi Jong An Jung In Young Yang | 2003 | KSME International Journal2003，，7： | 1 |
| 6 | Soluble adhesion molecules reflect endothelial cell activation in ischemic stroke and atherosclerosis显示文摘 | Kappelle LJ an Gi jn j | 1997 | Stoke1997，28，： | 1 |
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| 8 | Psychotherapyversus second-generation antidepressants in the treatment of de-pression 显示文摘 | Spielmans GI Berman MI Usitalo AN | 2011 | J Nerv Ment Dis2011，119，3： | 1 |
| 9 | Evidence for the association of peroxidases with the antioxidant effect of p-eoumaric acid in endo- thelial cells exposed to high glucose plus araehidonie acid 显示文摘 | Lee SJ Mun GI An SM | 2009 | BMB Rep2009，42，9： | 1 |
| 10 | ONASS ambiguity reso lution显示文摘 | WANG J An approach to GI | 2000 | Journal of Geodesy2000，74，5： | 1 |
| 11 | Birch bark ex- tract as therapy for chronic hepatitis C - a pilot study 显示文摘 | Shikov AN Djachuk GI Sergeev DV et at | 2011 | Phytomedicine2011，18，10： | 1 |
| 12 | Photobiological principles of therapeutic applications of laser radiation显示文摘 | Vladimirov YA Osipov AN Klebanov GI | 2004 | Biochemistry2004，69，： | 1 |
| 13 | Evidence for the associa- tion of peroxidases with the antioxidant effect of p - cou- marie acid in endothelial cells exposed to high glucose plus arachidonic acid 显示文摘 | Lee SJ Mun GI An SM | 2009 | BMP Rep2009，42，9： | 1 |
| 14 | Development of SSR markers for studies of diversity in the genus Fagopyrmn显示文摘 | Kyung Ho Ma Nam Soo Kim Gi An Lee | 2009 | The- or Appl Genet2009，119，7： | 1 |
| 15 | Synthesis and evaluation of a radioio-dinated bladder cancer specific peptide显示文摘 | Ha YS Lee HY An GI | | 0，，14： | 1 |
| 16 | Preparation of 3'-deoxy-3'fluorothymidine(FLT in ionic liquid显示文摘 | Moon BS Lee KC An GI | 2006 | J Label Compd Radiophann2006，49，： | 1 |
| 17 | Synthesis of amphiphilic star block copolymers of polystyrene with PEG core via ATRP-control of chain architecture and the formation of core-shell type globular structure 显示文摘 | An Sung Guk Li Guang Hwa Cho Chang Gi | 2006 | Polymer2006，47，： | 1 |
| 18 | Carpus trans- location into the ipsilateral ulna for distal radius recurrence giant cell tumour: A case report and literature review 显示文摘 | Ververidis AN Drosos GI Tilkeridis KE | 2015 | J Orthop2015，12，1： | 1 |
| 19 | Synthesis of amphiphilic star block copolymers of polystyrene with PEG core via ATRP-Control of chain architecture and the formation of core-shell type globularstructure显示文摘 | An Sung Guk Li Guang Hwa Cho Chang Gi | 2006 | Polymer2006，47，： | 1 |
| 20 | Nortriptline for treat- ment resistant depression显示文摘 | Nierenberg AN Papakostas GI Petersen T | 2003 | Journal of Clinical Psychiatry2003，64，1： | 1 |
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