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| 1 | Metallothionein mediates cardioprotection of isoliquiritigenin against ischemia-reperfusion through JAK2/STAT3 activation显示文摘瞄准:检验 isoliquiritigenin (ISL ) 是否能稀释心肌的 ischemiareperfusion (MI/R ) 在由通过双面联胎激酶的激活导致 metallothionein (MT ) 的老鼠的损害 2 (JAK 2 )/signal 变换器和抄写 3 的使活跃之物(STAT 3 ) 小径。方法:在老鼠的 MI/R 的试验性的模型被局部缺血和灌注的 30 min 为 2 h 产生。MT, COX-2,和 i NOS 的 mRNA 表示被 RT-PCR 测量。MT, JAK/STAT,细胞外的调整信号的激酶(英皇家空军之阶级最低之兵) ,和 Akt 的蛋白质表情被在一个 JAK 激酶禁止者的缺席或存在的西方的弄污决定, tyrphostin AG490 (1.0 mg/kg, iv,在局部缺血前的 1 h ) 。结果:有 ISL 的预告的处理显著地减少了导致 reperfusion 的心律不齐和心肌的梗塞尺寸的严厉。在 ISL 20 mg/kg 组,当时, lactate 脱氢酶(LDH ) 和肌酸酐磷酸激酶(CPK ) 的活动被 38.4% 和 51.3% 减少与车辆组相比。增加的 JAK 2/STAT 3 磷酸化被 MT 然而并非 COX-2 或 i NOS 的增加的合成在对待 ISL 的组伴随。AG490 能显著地削弱导致 ISL 的 cardioprotection 并且阻止 MT 表示和 JAK 2/STAT 的增加 3 磷酸化。结论:ISL 通过 JAK 2/STAT 的激活保护了 MI/R 损害 3 信号转导变异小径,它可能涉及调停起来 MT 的规定表示。 | Wei AN Jing YANG Ying AO | 2006 | Acta Pharmacologica Sinica2006，27，11： | 23 |
| 2 | MicroRNA-548 down-regulates host antiviral response via direct targeting of IFN-λ1显示文摘Interferon(IFN)-mediated pathways are a crucial part of the cellular response against viral infection.Type III IFNs,which include IFN-λ1,2 and 3,mediate antiviral responses similar to Type I IFNs via a distinct receptor complex.IFN-λ1 is more effective than the other two members.Transcription of IFN-λ1 requires activation of IRF3/7 and nuclear factor-kappa B(NF-κB),similar to the transcriptional mechanism of Type I IFNs.Using reporter assays,we discovered that viral infection in-duced both IFN-λ1 promoter activity and that of the 3′-untranslated region(UTR),indicating that IFN-λ1 expression is also regulated at the post-transcriptional level.After analysis with microRNA(miRNA)prediction programs and 3′UTR targeting site assays,the miR-NA-548 family,including miR-548b-5p,miR-548c-5p,miR-548i,miR-548j,and miR-548n,was identified to target the 3′UTR of IFN-λ1.Further study demonstrated that miRNA-548 mimics down-regulated the expression of IFN-λ1.In contrast,their inhibitors,the complemen-tary RNAs,enhanced the expression of IFN-λ1 and IFN-stimulated genes.Furthermore,miRNA-548 mimics promoted infection by enterovirus-71(EV71)and ve-sicular stomatitis virus(VSV),whereas their inhibitors significantly suppressed the replication of EV71 and VSV.Endogenous miRNA-548 levels were suppressed during viral infection.In conclusion,our results sug-gest that miRNA-548 regulates host antiviral response via direct targeting of IFN-λ1,which may offer a poten-tial candidate for antiviral therapy. | Yongkui Li Jiajia Xie Xiupeng Xu Jun Wang Fang Ao Yushun Wan Ying Zhu | 2013 | Protein & Cell2013，4，2： | 22 |
| 3 | Human umbilical cord blood stem cell transplantation for the treatment of chronic spinal cord injury Electrophysiological changes and long-term efficacy显示文摘Stem cell transplantation can promote functional restoration following acute spinal cord injury (injury time < 3 months), but the safety and long-term efficacy of this treatment need further exploration. In this study, 25 patients with traumatic spinal cord injury (injury time > 6 months) were treated with human umbilical cord blood stem cells via intravenous and intrathecal injection. The follow-up period was 12 months after transplantation. Results found that autonomic nerve functions were restored and the latent period of somatosensory evoked potentials was reduced. There were no severe adverse reactions in patients following stem cell transplantation. These experimental findings suggest that the transplantation of human umbilical cord blood stem cells is a safe and effective treatment for patients with traumatic spinal cord injury. | Liqing Yao Chuan He Ying Zhao Jirong Wang Mei Tang Jun Li Ying Wu Lijuan Ao Xiang Hu | 2013 | Neural Regeneration Research2013，8，5： | 14 |
| 4 | Water regime-nitrogen fertilizer incorporation interaction:Field study on methane and nitrous oxide emissions from a rice agroecosystem in Harbin, China显示文摘Water regime and nitrogen(N) fertilizer are two important factors impacting greenhouse gases(GHG) emission from paddy field, whereas their effects have not been well studied in cold region. In this study, we conducted a two-year field experiment to study the impacts of water regime and N fertilizer on rice yields and GHG emissions in Harbin, China, a cold region located in high latitudes. Our results showed that intermittent irrigation significantly decreased methane(CH_4) emission compared with continuous flooding, however,the decrement was far lower than the global average level. The N_2O emissions were very small when flooded but peaked at the beginning of the disappearance of floodwater. The N fertilizer treatments increased CH_4 emissions at low level(75 kg N/ha). But both CH_4 and N_2O emissions were uninfluenced at the levels of 150 kg N/ha and 225 kg N/ha. Rice yields increased under intermittent irrigation and were highest at the level of 150 kg N/ha. From our results, we recommended that the intermittent irrigation and 150 kg N/ha as the ideal water regime-nitrogen fertilizer incorporation for this area to achieve low GHG emissions without impacting rice yields. | Wenjun Dong Jia Guo Lijun Xu Zhifeng Song Jun Zhang Ao Tang Xijuan Zhang Chunxu Leng Youhong Liu Lianmin Wang Lizhi Wang Yang Yu Zhongliang Yang Yilei Yu Ying Meng Yongcai Lai | 2018 | Journal of Environmental Sciences2018，30，2： | 12 |
| 5 | Invasion biology of the cotton mealybug, Phenacoccus solenopsis Tinsley: Current knowledge and future directions显示文摘The cotton mealybug Phenacoccus solenopsis Tinsley(Hemiptera: Pseudococcidae), which is native to North America, emerged as a major invasive insect pest of multiple crops in Asia at the beginning of the 21 st century. Considering the economic significance of this insect and its rapid worldwide spread, we examined the possible factors driving its invasions. In this paper, we summarize the life history traits of P. solenopsis conceivably related to population development in invaded regions; these traits include its use of diverse host plants, reproductive capacity and mode, adaptation to temperature, response to food shortage, and insecticidal resistance. Then, focusing on the multiple trophic interactions that may promote or hinder invasion, we review the mutualistic relationship of this mealybug with ants and predation and parasitism by natural enemies. Finally, we suggest topics for future research and provide our perspective on the biological invasions of this mealybug. We speculate that specific biological factors associated with this mealybug, particularly its wide host range, high reproductive potential, evolved changes in life history traits, and mutualism with ants have played important roles in its invasions, allowing this pest to become established and rapidly increase its population upon its introduction into new regions. | TONG Hao-jie AO Yan LI Zi-hao WANG Ying JIANG Ming-xing | 2019 | Journal of Integrative Agriculture2019，18，4： | 10 |
| 6 | Cytocompatibility with osteogenic cells and enhanced in vivo anti-infection potential of quaternized chitosan-loaded titania nanotubes显示文摘Infection is one of the major causes of failure of orthopedic implants. Our previous study demonstrated that nanotube modification of the implant surface, together with nanotubes loaded with quaternized chitosan(hydroxypropyltrimethyl ammonium chloride chitosan, HACC), could effectively inhibit bacterial adherence and biofilm formation in vitro. Therefore, the aim of this study was to further investigate the in vitro cytocompatibility with osteogenic cells and the in vivo anti-infection activity of titanium implants with HACC-loaded nanotubes(NT-H). The titanium implant(Ti), nanotubes without polymer loading(NT), and nanotubes loaded with chitosan(NT-C) were fabricated and served as controls. Firstly, we evaluated the cytocompatibility of these specimens with human bone marrow-derived mesenchymal stem cells in vitro.The observation of cell attachment, proliferation, spreading, and viability in vitro showed that NT-H has improved osteogenic activity compared with Ti and NT-C. A prophylaxis rat model with implantation in the femoral medullary cavity and inoculation with methicillin-resistant Staphylococcus aureus was established and evaluated by radiographical, microbiological, and histopathological assessments. Our in vivo study demonstrated that NT-H coatings exhibited significant anti-infection capability compared with the Ti and NT-C groups. In conclusion, HACC-loaded nanotubes fabricated on a titanium substrate show good compatibility with osteogenic cells and enhanced anti-infection ability in vivo, providing a good foundation for clinical application to combat orthopedic implant-associated infections. | Ying Yang Haiyong Ao Yugang Wang Wentao Lin Shengbing Yang Shuhong Zhang Zhifeng Yu Tingting Tang | 2016 | Bone Research2016，4，3： | 9 |
| 7 | Soluble interleukin-6 receptor is elevated during influenza A virus infection and mediates the IL-6 and IL-32 inflammatory cytokine burst显示文摘流行性感冒 A 病毒(IAV ) 感染是一个主要全球公共的健康问题。然而,因素在调停包含了煽动性的反应到这感染和他们糟糕理解的关系遗体。这里,我们证明 IAV 感染刺激可溶的 IL-6 受体(sIL-6R ) 的表示,涉及 IL-6 发信号的多功能的蛋白质。有趣地, sIL-6R 表示 upregulated 它的自己的 ligand,支持 inflammatory cytokine IL-32 的 IL-6 和那些的层次。调停 shRNA sIL-6R 击倒压制的 IL-6 和 IL-32,显示这条规定在 IAV 感染期间依赖于 sIL-6R。而且,我们的结果证明 IL-32 参予禁止 sIL-6R 的一个否定反馈环当时 upregulating IL-6 表示在 IAV 感染期间。因此,我们证明 sIL-6R 是涉及对病毒的感染的尖锐煽动性的反应的一个关键细胞的因素。 | Jun Wang Qing Wang Tao Han Yong-Kui Li Sheng-Li Zhu Fang Ao Jian Feng Ming-Zhen Jing Li Wang Lin-Bai Ye Ying Zhu | 2015 | Cellular & Molecular Immunology2015，12，5： | 6 |
| 8 | Amyloid beta-peptide worsens cognitive impairment following cerebral ischemia-reperfusion injury显示文摘Amyloid β-peptide,a major component of senile plaques in Alzheimer’s disease,has been implicated in neuronal cell death and cognitive impairment.Recently,studies have shown that the pathogenesis of cerebral ischemia is closely linked with Alzheimer’s disease.In this study,a rat model of global cerebral ischemia-reperfusion injury was established via occlusion of four arteries;meanwhile,fibrillar amyloidβ-peptide was injected into the rat lateral ventricle.The Morris water maze test and histological staining revealed that administration of amyloidβ-peptide could further aggravate impairments to learning and memory and neuronal cell death in the hippocampus of rats subjected to cerebral ischemia-reperfusion injury.Western blot showed that phosphorylation of tau protein and the activity of glycogen synthase kinase 3βwere significantly stronger in cerebral ischemia-reperfusion injury rats subjected to amyloid β-peptide administration than those undergoing cerebral ischemia-reperfusion or amyloidβ-peptide administration alone.Conversely,the activity of protein phosphatase 2A was remarkably reduced in rats with cerebral ischemia-reperfusion injury following amyloidβ-peptide administration.These findings suggest that amyloidβ-peptide can potentiate tau phosphorylation induced by cerebral ischemia-reperfusion and thereby aggravate cognitive impairment. | Bo Song Qiang Ao Ying Niu Qin Shen Huancong Zuo Xiufang Zhang Yandao Gong | 2013 | Neural Regeneration Research2013，8，26： | 5 |
| 9 | Esophageal space-occupying lesion caused by Ascaris lumbricoides显示文摘Ascaris lumbricoides is the largest intestinal nematode parasite of man,which can lead to various complications because of its mobility.As the esophagus is not normal habitat of Ascaris,the report of esophageal ascariasis is rare.An old female presented with dysphagia after an intake of several red bean buns and haw jellies.The barium meal examination revealed a spherical defect in the lower esophagus.Esophageal bezoar or esophageal carcinoma was considered at the beginning.The patient fasted,and received fluid replacement treatment as well as some oral drugs such as proton pump inhibitor and sodium bicarbonate.Then upper gastrointestinal endoscopy was done to further confirm the diagnosis and found a live Ascaris lumbricoides in the gastric antrum and two in the duodenal bulb.The conclusive diagnosis was ascariasis.The esophageal space-occupying lesion might be the entangled worm bolus.Anthelmitnic treatment with mebendazole improved patient's clinical manifestations along with normalization of the radiological findings during a 2-wk follow-up.Authors report herein this rare case of Ascaris lumbricoides in the esophagus,emphasizing the importance of awareness of this parasitic infection as it often presents with different and unspecific symptoms. | Ping-Ping Zheng Bing-Yuan Wang Fei Wang Ran Ao Ying Wang | 2012 | World Journal of Gastroenterology2012，18，13： | 4 |
| 10 | Flipped classroom approach to ophthalmology clerkship courses for Chinese students of eight-year program显示文摘Background:The“flipped classroom”is a learner-centered approach that centers on delivering videos,podcasts or slide-based material to learners prior to a lecture or class session.The class session is then dedicated to discussion,analysis,and problem-solving activities.The aim of this study was to investigate whether the flipped classroom could be adapted to medical(ophthalmology)students learning about ocular trauma and to assess the impact of the flipped classroom on those students’performance and attitudes.Methods:Questionnaires(using a 4-point scale)were distributed to 93 fifth-year medical students at Sun Yat-sen University,and the data showed that the majority of students preferred the flipped classroom approach to the traditional lecture method.Results:The results of pre-and post-test scores were 14.35±3.404 and 20.37±4.356,which showed a significant improvement in students’performance after the flipped classroom was introduced(P<0.05).Conclusions:Student response to the flipped classroom strategy was largely positive,indicating that the strategy received a high level of approval in an ophthalmology clerkship course taken by medical students in China. | Ying Yang Chao-Chao Xu Yu Jia Yu-Xian Zou Yong Ao Zhe-Qian Huang Yu Cai Wei Xin Miao-Ling Li Yang-Fan Yang Hao-Tian Lin | 2017 | Annals of Eye Science2017，，1： | 4 |
| 11 | An Inverse Power Generation Mechanism Based Fruit Fly Algorithm for Function Optimization显示文摘As a novel population-based optimization algorithm, fruit fly optimization(FFO) algorithm is inspired by the foraging behavior of fruit flies and possesses the advantages of simple search operations and easy implementation. Just like most population-based evolutionary algorithms, the basic FFO also suffers from being trapped in local optima for function optimization due to premature convergence.In this paper, an improved FFO, named IPGS-FFO, is proposed in which two novel strategies are incorporated into the conventional FFO. Specifically, a smell sensitivity parameter together with an inverse power generation mechanism(IPGS) is introduced to enhance local exploitation. Moreover,a dynamic shrinking search radius strategy is incorporated so as to enhance the global exploration over search space by adaptively adjusting the searching area in the problem domain. The statistical performance of FFO, the proposed IPGS-FFO, three state-of-the-art FFO variants, and six metaheuristics are tested on twenty-six well-known unimodal and multimodal benchmark functions with dimension 30, respectively. Experimental results and comparisons show that the proposed IPGS-FFO achieves better performance than three FFO variants and competitive performance against six other meta-heuristics in terms of the solution accuracy and convergence rate. | LIU Ao DENG Xudong REN Liang LIU Ying LIU Bo | 2019 | Journal of Systems Science & Complexity2019，32，2： | 3 |
| 12 | Celastrol suppresses colorectal cancer via covalent targeting peroxiredoxin 1显示文摘As a terpenoids natural product isolated from the plant Thunder God Vine,Celastrol is widely studied for its pharmacological activities,including anti-tumor activities.The clinical application of Celastrol is strictly limited due to its severe side effects,whereas previously revealed targets and mechanism of Celastrol seldom reduce its in vivo toxicity via structural optimization.Target identification has a far-reaching influence on the development of innovative drugs,and omics data has been widely used for unbiased target prediction.However,it is difficult to enrich target of specific phenotype from thousands of genes or proteins,especially for natural products with broad promising activities.Here,we developed a text-mining-based web-server tool to enrich targets from omics data of inquired compounds.Then peroxiredoxin 1(PRDX1)was identified as the ROS-manipulating target protein of Celastrol in colorectal cancer.Our solved high-resolution crystal structure revealed the unique covalent binding mode of Celastrol with PRDX1.New derivative compound 19-048 with improved potency against PRDX1 and selectivity towards PRDX2~PRDX6 were synthesized based on crystal structure analysis.Both Celastrol and 19-048 effectively suppressed the proliferation of colorectal cancer cells.The anti-tumor efficacy of Celastrol and 19-048 was significantly diminished on xenograft nude mice bearing PRDX1 knock-down colorectal cancer cells.Several downstream genes of p53 signaling pathway were dramatically up-regulated with Celastrol or 19-048 treatment.Our findings reveal that the side effects of Celastrol could be reduced via structural modification,and PRDX1 inhibition is promising for the treatment of colorectal cancer. | Heng Xu Hongfang Zhao Chunyong Ding Defang Jiang Zijie Zhao Yang Li Xiaoyu Ding Jing Gao Hu Zhou Cheng Luo Guoqiang Chen Ao Zhang Ying Xu Hao Zhang | 2023 | Signal Transduction and Targeted Therapy2023，8，3： | 3 |
| 13 | Effects of infusing milk precursors into the artery on rumen fermentation in lactating cows显示文摘This experiment was conducted to investigate the effects of infusing milk precursors into the external pudic artery on rumen fermentation in lactating dairy cows. Eight multiparous Holstein cows were randomly assigned to Group A(experimental group) and Group B(control group) with 4 cows each. A2×4 complex factor crossover design was used. Cows in Group A were fed corn straw as the only roughage, and cows in Group B were fed mixed roughage. The experiment was divided into two periods.In the first period, cows in Group A, received treatments: 1) a basal infusate as a control(CSC); 2) a milk fat precursor infusion including C16:0, C18:0, C18:1 c9, C18:2 c6, C18:3 n3, acetic acid(CSF); 3) a milk protein precursor infusion including 16 amino acids(CSA); 4) the mixed infusion of milk fat and protein precursors(CSFA). And meanwhile, cows in Group B were infused the basal infusate as a control group. In the second period, the cows in both Groups A and B were crossed over, which cows in Group A were named as Group B and the cows originally in Group B were in Group A. The experimental results showed that cows in experimental group had higher ruminal pH compared with the control, and ruminal pH in CSC, CSF, CSA were significantly higher than those in their respective control group(P < 0.05). The concentration of ammonia nitrogen(NH_3-N) was significantly higher in CSA and CSFA compared with Group B(P < 0.05). We also observed that the infusion of mixed amino acids significantly increased the bacterial protein(BCP) content in rumen(P < 0.05) and influenced the rumen acetic acid concentration as well as the acetic to propionic ratio(P < 0.05). Milk fat precursors infusion significantly affected butyric acid concentration(P < 0.05). In addition, the content of lipopolysaccharide(LPS) in CSA was significantly higher than that in the control group(P < 0.05). It is concluded that the milk precursors infused into external pudic artery caused feedback effects on ruminal fermentation under the corn straw roughage conditions. The milk protein precursor increased the ruminal pH, the contents of BCP and acetic acid, which adjust rumen fermentation and improve milk performance. | Xianjue Wang Changjin Ao Khas-Erdene Shuaiwang Liu Chen Bai Fuquan Zhang Ying Zhang Peng Gao | 2016 | Animal Nutrition2016，，2： | 3 |
| 14 | The clinical relevance of MBL2 gene polymorphism and sepsis显示文摘Objective:To detect the clinical relevance of mannose-binding lectin 2(MBL2) gene polymorphism and sepsis in Chinese lived in Hainan island.Methods: Blood samples from 57 patients with sepsis and 69 patients without sepsis were collected in the ICU of several large hospitals in Hainan province. Genomic DNA was extracted from whole blood and then PCR purification product was sequenced and typed by 3730 sequencing analyzer. The concentration of MBIL2 in serum was detected by ELISA.Results: We found that genotype and allele distributions in two groups were in accordance with the Hardy-Weinberg Equilibrium. The frequency of GA genotype was significantly higher than that in non-sepsis group(P=0.013). A allele frequency in sepsis group was also much higher than that in non-sepsis group(P=0.028).Logister regression analysis showed that the patients who carried A allele were more prone to get sepsis than G allele carrier(P=0.014, OR=2,550, 95%CI=1.207-5.386). The MBL2 level in serum of sepsis patients with genotype GG and GA was significantly lower than that in non-sepsis group(P<0.05). In sepsis group, the MBL2 serum level of patients with genotype GA was obviously lower than that in patients with genotype GG(P<0.05).Conclusions: The variation of rs 1800450 G→A increased the incidence of sepsis and decreased the level of MBL2 in serum. | Shao-Wen Cheng Li-Na Xian Zhi-Xing Lin Xue Ao Jun-Yi Yao Ying-Qing Li Yong-Yan Li Xiu-Ru Li Wei-Cheng Wang Chuan-Zhu Lyu Ying Li | 2018 | Asian Pacific Journal of Tropical Medicine2018，11，3： | 2 |
| 15 | Rational design of a biosensor circuit with semi-log dose-response function in Escherichia coli显示文摘 | Haoqian Zhang Ying Sheng Qianzhu Wu Ao Liu Yuheng Lu Zhenzhen Yin Yuansheng Cao Weiqian Zeng Qi Ouyang | 2013 | Frontiers of Electrical and Electronic Engineering in China2013，8，3： | 2 |
| 16 | Microstructural changes during heating of super duplex stainless steel and its thermal deformation behavior显示文摘Microstructural changes during heating of highly alloyed Cr26Ni7 type super duplex stainless steel( SDSS2607) and its thermal deformation behavior were investigated. At different heating rates,the mechanism of phase transition from γ phase to δ phase and grow th modes of δ phase differed. Variations in microstructures for ascast SDSS2607 during heat preservation at 1 220 ℃ indicated two kinds of transformations from γ phase to δ phase.In-situ observations of microstructural changes during the tensile process at 1 050 ℃ showed a mutual coordination betw een γ and δ phases. When the true strain increased,the mutual coordination between γ and δ phases was damaged. Subsequently,cracks nucleated at the γ/δ interface. With the increase in temperature,the strength of ascast SDSS2607 decreased while its plasticity increased. Its thermoplasticity was poor,and the reduction in area of tensile specimens was less than 80%. When the deformation strain of hot compression increased,the stable deformation zone in the heat processing maps enlarged gradually. Moreover,the unstable deformation zones were extended. | ZHOU Candong AO Ying LIU Xiaorong | 2017 | Baosteel Technical Research2017，11，4： | 2 |
| 17 | Microstructure evolution of A380 aluminum alloy during rheological process under applied pressure显示文摘In squeeze casting process,the essence of mold-filling and feeding is rheological solidification of the alloy melt under pressure.Microstructure evolution is inevitable in this process,which affects the mold-filling and feeding in turn.In this work,the Archimedes spiral sample prepared by indirect squeeze casting was applied to investigate the microstructure evolution during the rheological process under pressure.The results showed that the primaryα-Al phase was transformed from fine rosette-like or granular structure to coarse platelet-like structure with the increase of spiral length.However,the primaryα-Al grain size of the starting point had a slight growing trend compared with that at the position of 140 mm away from the starting point.The volume fraction of the primaryα-Al phase increased from 45.57%to 70.35%along the spiral length direction,demonstrating that the experimental pressure improved the rheological ability of the alloy melt to some extent.Furthermore,the eutectic Si phase was varied from a fine granular or wormlike structure to a coarse platelet-like or needle-like structure,and the dispersion of eutectic Si particles was also varied along the spiral length direction.This microstructure evolution was mainly owing to the comprehensive action of rheological solidification and pressurized solidification.More specifically,the microstructure evolution strongly depended on the pressure and rheological velocity during the rheological process,however,the effect of rheological distance was relatively small. | Ying Wang Shu-ming Xing Xiao-hui Ao Ting-yue Wang | 2019 | China Foundry2019，16，6： | 2 |
| 18 | Exosomes secreted from cardiomyocytes suppress the sensitivity of tumor ferroptosis in ischemic heart failure显示文摘Heart failure(HF)patients in general have a higher risk of developing cancer.Several animal studies have indicated that cardiac remodeling and HF remarkably accelerate tumor progression,highlighting a cause-and-effect relationship between these two disease entities.Targeting ferroptosis,a prevailing form of non-apoptotic cell death,has been considered a promising therapeutic strategy for human cancers.Exosomes critically contribute to proximal and distant organ-organ communications and play crucial roles in regulating diseases in a paracrine manner.However,whether exosomes control the sensitivity of cancer to ferroptosis via regulating the cardiomyocyte-tumor cell crosstalk in ischemic HF has not yet been explored.Here,we demonstrate that myocardial infarction(MI)decreased the sensitivity of cancer cells to the canonical ferroptosis activator erastin or imidazole ketone erastin in a mouse model of xenograft tumor.Post-MI plasma exosomes potently blunted the sensitivity of tumor cells to ferroptosis inducers both in vitro in mouse Lewis lung carcinoma cell line LLC and osteosarcoma cell line K7M2 and in vivo with xenograft tumorigenesis model.The expression of miR-22-3p in cardiomyocytes and plasma-exosomes was significantly upregulated in the failing hearts of mice with chronic MI and of HF patients as well.Incubation of tumor cells with the exosomes isolated from post-MI mouse plasma or overexpression of miR-22-3p alone abrogated erastin-induced ferroptotic cell death in vitro.Cardiomyocyte-enriched miR-22-3p was packaged in exosomes and transferred into tumor cells.Inhibition of cardiomyocyte-specific miR-22-3p by AAV9 sponge increased the sensitivity of cancer cells to ferroptosis.ACSL4,a pro-ferroptotic gene,was experimentally established as a target of miR-22-3p in tumor cells.Taken together,our findings uncovered for the first time that MI suppresses erastin-induced ferroptosis through releasing miR-22-3p-enriched exosomes derived from cardiomyocytes.Therefore,targeting exosome-mediated cardiomyocyte/tumor pathological communication may offer a novel approach for the ferroptosis-based antitumor therapy. | Ye Yuan Zhongting Mei Zhezhe Qu Guanghui Li Shuting Yu Yingqi Liu Kuiwu Liu Zhihua Shen Jiaying Pu Yanquan Wang Changhao Wang Zhiyong Sun Qian Liu Xiaochen Pang Ao Wang Zijing Ren Tong Wang Ying Liu Jinhuan Hong Jiajie Xie Xin Li Zhonghua Wang Weijie Du Baofeng Yang | 2023 | Signal Transduction and Targeted Therapy2023，8，4： | 2 |
| 19 | Phosphorylation of tau protein over time in rats subjected to transient brain ischemia显示文摘Transient brain ischemia has been shown to induce hyperphosphorylation of the microtubule-associated protein tau.To further determine the mechanisms underlying these processes,we investigated the interaction between tau,glycogen synthase kinase(GSK)-3βand protein phosphatase 2A.The results confirmed that tau protein was dephosphorylated during brain ischemia;in addition,the activity of GSK-3βwas increased and the activity of protein phosphatase2A was decreased.After reperfusion,tau protein was hyperphosphorylated,the activity of GSK-3βwas decreased and the activity of protein phosphatase 2A remained low.Importantly,the interaction of tau with GSK-3βand protein phosphatase 2A was altered during ischemia and reperfusion Lithium chloride could affect tau phosphorylation by regulating the interaction of tau with GSK-3βand protein phosphatase 2A,and improve learning and memory ability of rats after transient brain ischemia.The present study demonstrated that it was the interaction of tau with GSK-3βand protein phosphatase 2A,rather than their individual activities,that dominates the phosphorylation of tau in transient brain ischemia.Hyperphosphorylated tau protein may play an important role in the evolution of brain injury in ischemic stroke.The neuroprotective effects of lithium chloride partly depend on the inhibition of tau phosphorylation during transient brain ischemia. | Bo Song Qiang Ao Zhen Wang Weiqiang Liu Ying Niu Qin Shen Huancong Zuo Xiufang Zhang Yandao Gong | 2013 | Neural Regeneration Research2013，8，34： | 2 |
| 20 | NANOG Alleviates the Damage of Human Hair Follicle Mesenchymal Stem Cells Caused by H_2O_2 through Activation of AKT Pathway显示文摘Objective To explore the protective effect of NANOG against hydrogen peroxide(H_2O_2)-induced cell damage in the human hair follicle mesenchymal stem cells(hHF-MSCs). Methods NANOG was expressed from a lentiviral vector, pLVX-IRES-ZsGreen. NANOG hHF-MSCs and vector hHF-MSCs were treated with 400 μmol/L hydrogen peroxide(H_2O_2) for 2 h, the cell survival rate, cell morphology, ROS production, apoptosis and expression of AKT, ERK, and p21 were determined and compared. Results Our results showed that NANOG could activate AKT and upregulate the expression of p-AKT, but not p-ERK. When treated with 400 μmol/L H_2O_2, NANOG hHF-MSCs showed higher cell survival rate, lower ROS production and apoptosis, higher expression of p-AKT, higher ratio of p-AKT/AKT. Conclusion Our results suggest that NANOG could protect hHF-MSCs against cell damage caused by H_2O_2 through activating AKT signaling pathway. | SHI Jia Hong ZUO Kui Yang ZHANG Ying Yao WANG Bo HAN Xing LIAN Ao Bo LIU Jin Yu | 2019 | Biomedical and Environmental Sciences2019，32，4： | 2 |
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