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| 1 | Hepatitis C virus in human B lymphocytes transformed by Epstein-Barr virus in vitro by in situ reverse transcriptase-polymerase chain reaction显示文摘AIM To study persistence and replication ofheltitis C virus (HCV) in patients' peripheralblood mononuclear cells (PBMC) cultured invitro.METHODS Epstein-Barr virus (EBV) was usedto transform the hepatitis C virus from a HCVpositive patient to permanent lymphoblastoidcell lines (LCL). Positive and negative HCV RNAstrands of the cultured cells and growth mediawere detected by reverse transcriptase-polymerase chain reaction ( RT-PCR ) eachmonth. Core and NS5 proteins of HCV werefurther tested using immunohistochemical SPmethod and in situ RT-PCR.RESULTS HCV RNA positive strands wereconsistently detected the cultured cells for oneyear. The negative-strand RNA in LCL cells andthe positive-strand RNA in supernatants wereobserved intermittently. Immunohistochemicalresults medicated expression of HCV NS3 and Cproteins in LCL cytoplasm mostly. The positivesignal of PCR product was dark blue and mainlylocalized to the LCL cytoplasm. The RT-PCRsignal was eliminated by overnight RNasedigestion but not DNase digestion.CONCLUSION HCV may exist and remainfunctional in a cultured cell line for a longperiod. | Ji Lin Cheng Bao Ling Liu Yi Zhang Wen Bin Tong Zheng Yan Bai Fang Feng Institute of Hepatology,Peoples Hospital,Medical Center of Beijing University,Beijing 10(X)44,China | 2001 | World Journal of Gastroenterology2001，7，3： | 11 |
| 2 | 水热合成法对纳米氧化锡粉体粒径和形貌的控制研究显示文摘利用氯化锡和氨水作为反应试剂,通过水热合成技术制备了近球形,棒状,椭球形,六角形等粉体形貌和粒径范围从4nm至120nm的纳米氧化锡粉体,并对水热合成条件对粉体的粒径和形貌的影响进行了研究。所制备的粉体的XRD分析结果显示,合成温度在160℃以上并且合成时间在3h以上,粉体全部具有氧化锡晶体结构。利用透射电镜对粉体的粒径和形貌观察发现,二氧化锡粉体的形貌和粒径可通过改变溶液的浓度,合成温度与合成时间进行有效的控制,其中合成温度与溶液浓度不仅可以控制合成粉体的粒径,而且可以控制粉体的形貌。 | 王东新 钟景明 孙本双 BAO X 张建荣 | 2008 | 无机化学学报2008，24，6： | 11 |
| 3 | 茶叶摄入与中国成年人高密度脂蛋白胆固醇浓度纵向变化的关系显示文摘茶叶摄入与高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)浓度的年龄相关变化之间的关系仍不清楚。该研究目的是探索茶摄入量与HDL-C浓度纵向变化之间的关系。研究人员通过调查问卷评估基线(2006年)的茶叶消费量,并在2006、2008、2010和2012年测量了未患有心血管病或癌症. | 刘莉 叶鹏 Huang S Li J Wu Y Ranjbar S Xing A Zhao H Wang Y Shearer GC Bao L Lichtenstein AH Wu S Gao X | 2018 | 中华高血压杂志2018，26，8： | 5 |
| 4 | Simultaneous multi-tear exclusion:an optimal strategy for type B thoracic aortic dissection initially proved by a single center's 8 years experience显示文摘背景 Endovascular 接枝stent 广泛地被用来对待胸的大动脉的 dissection.However ,很少信息都不关于跟随八年用为斯坦福的治疗的同时的多眼泪排除的经验打 B 胸的大动脉的解剖导致到1月2006,29日类型 B 胸的大动脉的解剖病人的从1998年9月的没有不利 events.Methods 的成功的大动脉的改变的多重 tears.This 报告细节的同时的排除的结果是可得到的( 24 个人, 5 | YUAN Liang-xi BAO Jun-min ZHAO Zhi-qing FENG Xiang OU Le-feng FENG Rui LU Qing-sheng MEI Zhi-jun JING Zai-ping Department of Vascular Surgery,Changhai Hospital,Second Military Medical University Institute of Vascular Surgery of Chinese People’s Liberation Army Clinic Center of Vascular Diseases of Shanghai National Key Department of Vascular Surgery of China Shanghai 200433,China (Yuan LX,Bao JM,Zhao ZQ,Feng X,Ou LF,Feng R,Lu QS,Mei ZJ and Jing ZP) | 2007 | Chinese Medical Journal2007，，24： | 3 |
| 5 | 纳米光子结构软膜紫外光固化纳米压印(英文)显示文摘紫外光固化纳米压印是实现纳米结构批量复制的一种新技术.其特点是低成本和高分辨,而且可以达到极高的套刻精度.为了得到大面积图案的均匀复制,可用聚二甲基硅氧烷(PDMS)制备透光的压印软模板.其母版图案可由高分辨率电子束曝光和反应离子刻蚀的方法在硅片基底上获得,然后用浇注的方法将图案转移到PDMS上.本实验特别发展了紫外光固化纳米压印适用于软膜压印的双层膜图型转移技术.该双层膜由廉价的光胶和聚甲基丙烯酸甲脂(PMMA)构成.对光胶层的压印可用普通的光学曝光仪实现.然后再将图案用反应离子刻蚀的方法转移到PMMA层中.为了证明方案的可行性,在两种不同材料的半导体基片上压印了三角晶格的光子晶体和准晶结构的图案,并用剥离的方法将它们转移到金属薄膜上,最后成功地进行了硅片刻蚀实验.相信这一纳米制做方法对大面积纳米光子结构和光学集成芯片的制造是普遍适用的. | SHI J WANG Z M BAO K PEROZ C BELOTTI M XU L P LUO C X SUN M H ZHANG B JI H OUYANG Q CHEN Y | 2006 | 纳米技术与精密工程2006，4，3： | 2 |
| 6 | Strong ultraviolet photoluminescence from silicon oxide films prepared by magnetron sputtering显示文摘 | SONG H Z BAO X M LIN S | 1998 | Appl Phys Lett1998，78，： | 2 |
| 7 | Microsatellite markers derived from bay scallop (Argopecten irradians) expressed sequence tags显示文摘 | Zhan A B Bao Z M Wang X L etal | 2005 | Fisheries Science2005，71，6： | 1 |
| 8 | Combined distributed temperature and strain sensor based on Brillouin loss in an optical fiber 显示文摘 | BAO X WEBB D J JACKSON D A | 1994 | Opt Lett1994，19，2： | 1 |
| 9 | Alkaline syenites in eastern Cathaysia (South China): Link to Permian-Triassic transtension显示文摘 | Wang Q Li J W Jian P Zhao Z H Xiong X L Bao Z W Xu J F Li C F Ma J L | 2005 | Emth Planet Sci Lett2005，230，34： | 1 |
| 10 | A new feature vector using selected bispectra for signal classification with application in radar target recognition显示文摘 | ZHANG X D SHI Y BAO Z | 2001 | IEEE Transactions on SignalProcessing2001，49，9： | 1 |
| 11 | Poly(ADP-ribose)polymerase activationand brain edema formation by hemoglobin after intracerebral in rats显示文摘 | Bao X Wu G Hu S | 2008 | Acta Neurochir Supple2008，105，： | 1 |
| 12 | Reexpression of the tumor suppressor gene ARHI induces apoptosis in ovarian and breast cancer cells through a caspase-independent calpain-dependent pathway 显示文摘 | Bao J J Le X F Wang R Y | 2002 | Cancer Res2002，62，24： | 1 |
| 13 | Variable speed control of wind turbines using nonlinear and adaptive algorithms 显示文摘 | SONG Y D DHINAKARAN B BAO X | 2000 | Wind Engineering and Industrial Aerodynamics2000，85，5： | 1 |
| 14 | Distributed optical fiber vibration sensor based on spectrum analysis of polarization-OTDR system显示文摘 | ZHANG Z Y BAO X Y | 2008 | Optics Express2008，16，14： | 1 |
| 15 | The technology of extracting vanadium from stone coal in Chi- na: history, current status and future prospects 显示文摘 | Zhang Y M Bao S X Liu T Chen T J Huang J | 2011 | Hydrometallurgy2011，109，： | 1 |
| 16 | MicroRNA-34a overcomes HGFmediated gefitinib resistance in EGFR mutant lung cancer cells partly by targeting MET显示文摘 | Zhou JY Chen X Zhao J Bao Z Chen X Zhang P | 2014 | Cancer Lett2014，351，2： | 1 |
| 17 | Observation of narrow line width spikes in the coherent Brillouin random fiber laser显示文摘 | M Pang X Y Bao L Chen | 2013 | Opt Lett2013，38，11： | 1 |
| 18 | Inflammation and diabetic nephropathy显示文摘 | Bao X Shandong YE | 2012 | International Journal of Pathology&Clinical Medicine2012，6，6： | 1 |
| 19 | Role of delivery and trafficking of delta-opioid peptide receptors in opioid analgesia and toler- ance 显示文摘 | Zhang X Bao L Guan JS | 2006 | Trends Pharmacol Sci2006，27，6： | 1 |
| 20 | Magnetocaloric properties of La( Fe, Si) 13-based material and its hydride prepared by industrial mischmetal 显示文摘 | Bao L F Hu F X Chen L | 2012 | Applied Physics Letters2012，101，16： | 1 |
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