|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | High miR-196a levels promote the oncogenic phenotype of colorectal cancer cells显示文摘AIM: To analyze the relevance of the microRNA miR-196a for colorectal oncogenesis. METHODS: The impact of miR-196a on the restriction targets HoxA7, HoxB8, HoxC8 and HoxD8 was analyzed by reverse transcription polymerase chain reaction (RT-PCR) after transient transfection of SW480 cancer cells. The miR-196a transcription profile in colorectal cancer samples, mucosa samples and diverse cancer cell lines was quantifi ed by RT-PCR. Transiently miR-196a-transfected colorectal cancer cells were used for diverse functional assays in vitro and for a xenograft lung metastasis model in vivo. RESULTS: HoxA7, HoxB8, HoxC8 and HoxD8 were restricted by miR-196a in a dose-dependent and gene-specific manner. High levels of miR-196a activated the AKT signaling pathway as indicated by increased phosphorylation of AKT. In addition, high levels of miR-196a promoted cancer cell detachment,migration, invasion and chemosensitivity towards platin derivatives but did not impact on proliferation or apoptosis. Furthermore, miR-196a increased the development of lung metastases in mice after tail vein injection. CONCLUSION: miR-196a exerts a pro-oncogenic influence in colorectal cancer. | Carl Christoph Schimanski Kirsten Frerichs Fareed Rahman Martin Berger Hauke Lang Peter R Galle Markus Moehler Ines Gockel | 2009 | World Journal of Gastroenterology2009，15，17： | 40 |
| 2 | Bcl-x_L and Myeloid cell leukaemia-1 contribute to apoptosis resistance of colorectal cancer cells显示文摘AIM: To explore the role of Bcl-xL and Myeloid cell leukaemia (Mcl)-1 for the apoptosis resistance of colorectal carcinoma (CRC) cells towards current treat-ment modalities. METHODS: Bcl-xL and Mcl-1 mRNA and protein ex-pression were analyzed in CRC cell lines as well as human CRC tissue by Western blot,quantitative PCRand immunohistochemistry. Bcl-xL and Mcl-1 protein expression was knocked down or increased in CRC cell lines by applying specific siRNAs or expression plas-mids,respectively. After modulation of protein expres-sion,CRC cells were treated with chemotherapeutic agents,an antagonistic epidermal growth factor recep-tor (EGFR1) antibody,an EGFR1 tyrosine kinase inhibi-tor,or with the death receptor ligand TRAIL. Apoptosis induction and cell viability were analyzed. RESULTS: Here we show that in human CRC tis-sue and various CRC cell lines both Bcl-xL and Mcl-1 are expressed. Bcl-xL expression was higher in CRC tissue than in surrounding non-malignant tissue,both on protein and mRNA level. Mcl-1 mRNA expression was significantly lower in ma-lignant tissues. However,protein expression was slightly higher. Viability rates of CRC cells were significantly decreased after knock down of Bcl-xL expression,and,to a lower extent,after knock down of Mcl-1 expression. Furthermore,cells with reduced Bcl-xL or Mcl-1 expression was more sensitive towards oxaliplatin-and irinotecan-induced apoptosis,and in the case of Bcl-xL also towards 5-FU-induced apoptosis. On the other hand,upregulation of Bcl-xL by transfec-tion of an expression plasmid decreased chemothera-peutic drug-induced apoptosis. EGF treatment clearly induced Bcl-xL and Mcl-1 expression in CRC cells. Apop-tosis induction upon EGFR1 blockage by cetuximab or PD168393 was increased by inhibiting Mcl-1 and Bcl-xL expression. More strikingly,CD95-and TRAIL-induced apoptosis was increased by Bcl-xL knock down. CONCLUSION: Our data suggest that Bcl-xL and,to a lower extent,Mcl-1,are important anti-apoptotic factors in CRC. Specific downregulation of Bcl-xL is a promising approach to sensitize CRC cells towards chemotherapy and targeted therapy. | Henning Schulze-Bergkamen Roland Ehrenberg Lothar Hickmann Binje Vick Toni Urbanik Christoph C Schimanski Martin R Berger Arno Schad Achim Weber Steffen Heeger Peter R Galle Markus Moehler | 2008 | World Journal of Gastroenterology2008，14，24： | 4 |
| 3 | Coexpression of receptor-tyrosine-kinases in gastric adenocarcinoma-a rationale for a molecular targeting strategy?显示文摘AIM: To define the (co-)expression pattern of target receptor-tyrosine-kinases (RTK) in human gastric adenocarcinoma. METHODS: The (co-)expression pattern of VEGFR1-3,PDGFRα/b and EGFR1 was analyzed by RT-PCR in 51 human gastric adenocarcinomas. In addition,IHC staining was applied for confirmation of expression and analysis of RTK localisation. RESULTS: The majority of samples revealed a VEGFR1 (98%),VEGFR2 (80%),VEGFR3 (67%),PDGFRα (82%) and PDGFRβ(82%) expression,whereas only 62% exhibited an EGFR1 expression. 78% of cancers expressed at least four out of six RTKs. While VEGFR1-3 and PDGFRα revealed a predominantly cytoplasmatic staining in tumor cells,accompanied by an additional nuclear staining for VEGFR3 ,EGFR1 was almost exclusively detected on the membrane of tumor cells. PDGFRβ was restricted to stromal pericytes,which also depicted a PDGFRα expression.receptor-tyrosine-kinases coexpression in gastric adenocarcinoma and might therefore encourage an application of multiple-target RTK-inhibitors within a combination therapy. | Daniel Drescher Markus Moehler Ines Gockel Kirsten Frerichs Annett Müller Friedrich Dünschede Thomas Borschitz Stefan Biesterfeld Martin Holtmann Thomas Wehler Andreas Teufel Kerstin Herzer Thomas Fischer Martin R Berger Theodor Junginger Peter R Galle Carl C Schimanski | 2007 | World Journal of Gastroenterology2007，13，26： | 4 |
| 4 | B-type natriuretic peptide predicts sudden death in patients with chronic heart failure显示文摘 | Berger R Huelsman M Strecker K | | 0，，： | 2 |
| 5 | Supercritical fluids crystallization of budesonide and flunisolide 显示文摘 | Velaga S E Berger R Carlfors J | 2002 | Pharm Res2002，19，10： | 1 |
| 6 | Geology and structural evolution of the Muruntau gold deposit, Kyzylkum Desert, Uzbekistan 显示文摘 | Berger B R Kurbanov N K | 1996 | Ore Geology Reviews1996，11，： | 1 |
| 7 | Lithium ion induced surface reativity change on MgO nanoparticles显示文摘 | BERGER R SCHUH J STERRER M DIWALD O KNOZINGER E | 2007 | J Catalysis2007，247，： | 1 |
| 8 | A novel,automated method of temperature cycling to produce cryoprecipitate显示文摘 | Rock G Berger R Lange J eta1 | 2001 | Transfusion2001，41，2： | 1 |
| 9 | Antioxidant activity of extracts from roasted wheat germ显示文摘 | Krings U El Saharty Y S Berger R G | 2000 | Food Chemistry2000，71，1： | 1 |
| 10 | The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533显示文摘 | PRIDMORE R D BERGER B DESIERE F | 2004 | Proc Natl Acad Sci USA2004，，8： | 1 |
| 11 | Some Explorations in Initial Interactions and Beyond: Towards A Developmental Theory of Interpersonal Communication 显示文摘 | Berger C R Calabrese R | 1975 | Human Communication Research1975，，1： | 1 |
| 12 | Mean platelet volume as a predictor of cardiovascular risk: a systematic review and meta-anal- ysis 显示文摘 | Chu S G Becker R C Berger P B | 2010 | J Thromb Haemost2010，8，1： | 1 |
| 13 | Is cervical elongation associated with pelvic organ prolapse? 显示文摘 | Berger MB Ramanah R Guire KE | 2012 | Int Urogyneeol J2012，23，： | 1 |
| 14 | Matrix metalloproteinase-9 triggers the angiogenic switch during carcinogenesis显示文摘 | Bergers G Brekken R McMahon G | | 0，，10： | 1 |
| 15 | Toll-like receptor 9 expression in breast and ovarian cancer is associated with poorly differentiated tumors显示文摘 | Berger R Fiegl H Goebel G | | 0，，04： | 1 |
| 16 | Signal processing for passive radar using OFDM waveforms显示文摘 | BERGER C R DEMISSIE B HECKENBACH J | 2010 | IEEE Journal of Selected Topics in Signal Processing2010，4，1： | 1 |
| 17 | Bilateral intralobar pulmonary sequestration in a newborn, case report and review of the literature on bilateral pulmonary sequestrations显示文摘 | Stern R Berger S Casauha C | 2007 | J Pediatr Surg2007，42，4： | 1 |
| 18 | Transforming growth factor - beta receptor mutations and pulmonary arterial hypertension in childhood 显示文摘 | HARRISON R E BERGER R HAWORTH S G | 2005 | Circulation2005，111，4： | 1 |
| 19 | The genome sequence of probiotic intestine bacterium Lactobacillus johnsonii NCC533 显示文摘 | Pridmore R D Berger B Desiere F | 2004 | Pro Nat Acad Sci USA2004，101，8： | 1 |
| 20 | Identification of inflicted traumatic brain injury in well-appearing infants using serum and cerebrospinalmarkers:A possible screening tool显示文摘 | Berger R P Dulani T Adelson P D | 2006 | Pediatrics2006，117，2： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员