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| 1 | Quantitative analysis of vascular endothelial growth factor, microvascular density and their clinicopathologic features in human hepatocellular carcinoma显示文摘BACKGROUND: Angiogenesis is known to be essential to the survival, growth, invasion, and metastasis of tumor cells. Vascular endothelial growth factor (VEGF) are an important angiogenic factor regulating tumor angiogenesis, but its significance and tumor pathologic features are un- clear in hepatocellular carcinoma (HCC). In the present study, we analyzed expression of tissue VEGF, alteration of microvascular density (MVD) in microvessel angiogenesis, development and metastasis of HCC, and level of serum VEGF in differential diagnosis of benign and malignant liv- er diseases. METHODS: Tumor specimens were prospectively collected from HCC patients undergoing resection. Total RNAs were extracted and the expression levels were detected from different parts of HCC tissues. The cellular distributions of VEGF and MVD of liver tumors and their paracancerous and distal cancerous tissues were investigated by streptavi- din peroxidase (S-P) immunohistochemistry, respectively. The VEGF levels of circulating blood and hepatoma tissues were measured by enzyme-linked immunosorbent assay. RESULTS: The incidence of VEGF expression was 63.9% in HCCs (23/36 cases), 78.3% in non-encapsulated HCCs (18/23), and 90.9% in HCCs with extrahepatic metastasis (10/11), respectively. The VEGF expression was tightly correlated with MVD (P <0.01). The MVD in HCC with metastasis, low differentiation or non-encapsulation was significantly higher than that in HCC with intact capsule, high differentiation, or no metastasis. No significant diffe- rence was found between VEGF, MVD, tumor size, and hepatitis virus infection. The level of total RNA in HCC tis- sues was significantly lower but the VEGF level significantly higher than those in paracancerous or distal cancerous ones (P<0.01). The abnormal expression levels of VEGF in sera of HCC patients were directly correlated with the me- tastasis and recurrence of tumors. CONCLUSION: The high expression of VEGF and abnor- mality of tissue MVD are useful predictors for vascular inva- sion and metastasis of liver tumors. | Deng-Fu Yao, Xin-Hua Wu, Yong Zhu, Gong-Sheng Shi, Zhi-Zhen Dong, Deng-Bing Yao, Wei Wu, Li-Wei Qiu and Xian-Yong Meng Nantong, China Research Center of Clinical Molecular Biology , Department of Pathology and Department of Gastroenterology , Affiliated Hos- pital of Nantong University Department of Diagnostics , and Institute of Neurosciences , Nantong University Nantong 226001, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，2： | 81 |
| 2 | MAPK signal pathways in the regulation of cell proliferation in mammalian cells显示文摘MAPK families play an important role in complex cellular programs like proliferation, differentiation, development, transformation, and apoptosis. At least three MAPI(families have been characterized: extracellular signal-regulated kinase (ERK), Jun kinase (JNK/SAPK) and p38 MAPK. The above effects are fulfilled by regulation of cell cycle engine and other cell proliferation related proteins. In this paper we discussed their functions and cooperation with other signal pathways in regulation of cell proliferation. | WEI ZHANG, Hui Tu LIU The Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Sciences, Beijing Normal University, Beijing 100875, China | 2002 | Cell Research2002，12，1： | 107 |
| 3 | Growth inhibition and apoptosis induction Sulindac on Human gastric cancer cells显示文摘AIM: To evaluate the effects of sulindac in inducing growth inhibition and apoptosis of human gastric cancer cells in comparison with human hepatocellular carcinoma (HCC)cells.METHODS: The human gastric cancer cell lines MKN45 and MKN28 and human hepatocellular carcinoma cell lines HepG2and SMMC7721 were used for the study. Anti-proliferative effect was measured by MTT assay, and apoptosis was determined by Hoechst-33258 staining, electronography and DNA fragmentation. The protein of cyclooxygenase-2 (COX(2) and Bcl-2 were detected by Westem dot blotting.RESULTS: Sulindac could initiate growth inhibition and apoptosis of MKN45, MKN28, HepG2 and SMMC7721 cells in a dose-and time-dependent manner. Growth inhibitory activity and apoptosis were more sensitive in HepG2 cells than in SMMC7721 cells, MKN45 and MKN28 cells. After 24hours incubation with sulindac at 2mmol. L-1 and 4mmol.L-1, the level of COX-2 and Bcl-2 protein were lowered in MKN45, SMMC7721 and HepG2 cells but not in MKN28 cells.CONCLUSION: Sulindac could inhibit the growth of gastric cancer cells and HCC cells effectively in vitro by apoptosis induction, which was associated with regression of COX-2and Bcl-2 expression. The growth inhibition and apoptosis of HCC cells were greater then that of human gastric cancer cells. The different effects of apoptosis in gastric cancer cells may be related to the differentiation of the cells. | Yun-Lin Wu~1 Bo Sun~1 Xue-Jun Zhang~2 Sheng-Nian Wang~2 Heng-Yi He~2 Min-Min Qiao~1 Jie Zhong~1 Jia-Yu Xu~1 1 Department of Gastroenterology,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China2 Institude of Biochemistry and Cell Biology,Shanghai Institues for Biological Sciences,Chinese Academy of Sciences.Shanghai 200025,China | 2001 | World Journal of Gastroenterology2001，7，6： | 64 |
| 4 | Progress in the bionic study on anti-adhesion and resistance reduction of terrain machines显示文摘The theoretical studies of bionics of machinery have great scientific significance, and the development of bionic machines has large practical values in the field of engineering and technology. Through the rigorous selection process of evolution, the survived living organisms have successfully developed outstanding abilities to adapt to their surroundings and to reproduce their offspring. In this review,we interpreted the fundamental principles of anti-adhesion and anti-resistance of soil animals by reviewing the current status in this research field and summarizing the work of the research group at Jilin University of China in the past decades. The principles and technologies used in morphology bionics,electric-osmosis bionics,flexibility bionics,configuration bionics and coupling bionics were examined.Finally,the applications of the engineering bionics and their extensive prospects were introduced. | REN LuQuanKey Laboratory for Terrain-Machine Bionics Engineering of Ministry of Education, School for Biology and Agricultural Engineering, Jilin University, Changchun 130025, China | 2009 | Science China(Technological Sciences)2009，52，2： | 64 |
| 5 | Inhibitory effect of IGF-Ⅱ antisense RNA on malignant phenotype of hepatocellular carcinoma显示文摘INIRODUCTIONAccording to the therapeutic effect and strategy ofantisense RNA for hepatoccllular carcinoma(HCC),we have specifically synthesized partialcDNA of human insulin-like growth factor Ⅱ(IGF-Ⅱ)and constructed IGF-Ⅱ cDNA antisenseeukaryotic expression vector.The constructedvector was introduced into hepatoma cell lineSMMC-7721 to block the intrinsic IGF-Ⅱexpression.The biological behavior changes ofhepatoma cells were observed.All these | Dong Hua Yang Ming Qing Zhang Jiang Du Chong Xu Oiao Ming Liang Ji Fang Mao Han Rong Qin Zi Rong Fan Department of Gastroenterology,Zhujiang Hospital,the First Military Medical University,Guangzhou 510282,China Laboratory of Molecular Biology,Zhujiang Hospital,the First Military Medical University,Guangzhou,China Departrnent of Biochemistry,the Second Military Medical University,Shanghai,China | 2000 | World Journal of Gastroenterology2000，6，2： | 54 |
| 6 | Get effective polyclonal antisera in one month显示文摘According to the traditional immunization procedure, after the first injection of the sample A (emulsion of aimed antigen and Freund's complete adjuvant) to immunize rabbit, successive injections of the sample B (emulsion of aimed antigen and Freund's incomplete adjuvant) were followed every 2-4 weeks. In general,high titer of the corresponding polyclonal antisera will be observed after 4-5 injections of sample B in 3-4months. This report presents a simply modified procedure that was able to stimulate the antisera formation in one month and achieve enough avidity to satisfy either Western blot or immunohistochemistry analysis.It just applied an additional injection of the sample A to the rabbit at the 3rd day after the primary immunization injection. You could gain the high titer of the antisera right after the first sample B injection in one month. This method has produced the desired results in three different recombinant antigens with different molecular weight (5.9 KD-55 KD) expressed from prokaryotic or eukaryotic cells. | YUAN XIN HU, Ju YUAN QUO, Lu SHEN, YAN CHEN, Zu CHUAN ZHANG, YONG LIAN ZHANGState Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, | 2002 | Cell Research2002，12，2： | 45 |
| 7 | Invasive mechanism and control strategy of Ageratina adenophora (Sprengel)显示文摘In order to ascertain the invasive mechanism and control strategy of the invasive Crofton weed, Ageratina adenophora, its ecological adaptability and population differentiation,the formation of single dominant population, displacement of native plants and sustainable management strategies were investigated. The present results helped to clarify and explain such issues as the adaptability post invasion,interaction and competition between inter-and intra-species and community resistance, thereby providing important references to researches on other invasive alien species. | WAN FangHao1, LIU WanXue1, GUO JianYing1, QIANG Sheng2, LI BaoPing2, WANG JinJun3, YANG GuoQing1, NIU HongBang1, GUI FuRong1, HUANG WenKun1, JIANG ZhiLin1 & WANG WenQi3 1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China 2College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China 3Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China | 2010 | Science China(Life Sciences)2010，53，11： | 42 |
| 8 | Studies on the integration of hepatitis B virus DNA sequence in human sperm chromosomes显示文摘Aim:To study the integration of hepatitis Bvirus(HBV)DNAinto sperm chromosomes in hepatitsBpatients and the features of its integration.Methods:Sperm chromosomes of 14subjects(5healthy controls and9HBpatients,including1acute hepatitis B,2chronic active hepatitisB,4chronic persistent hepatitsB,2HBsAg chronic carriers with no clinical symptoms)were prepared using imterspecific in vitro fertilization between zona-free hamster oocytes and human spermatozoa.Fluosescence in situ hybridization(FISH)to sperm chromosome spreads was carried out with biotin-labeled full length HBVDNAprobe to detect the specificHBVDNA sequences in the sperm chromosomes.Results:Specific fluorescent signal spots for HBVDNAwere seen iv sperm chromosomes of one patient with chronic persistent hepatitisB.In9(9/42)sperm chromosome complements containing fluorescent signal spots,one presented5obvious FISHspots and the others2to4signals.The fluorescence intensity showed significant difference among the signal spots.The distribution of signal sites among chromosomes seems to be random.Con clusion:HBV could integrate into human sperm chromosomes.Results suggest that the possibility of vertical transmission of HBVvia the germ line tothe next generation is present. | Jian-Min HUANG~(1,*), Tian-Hua HUANG~1, Huan-Ying QIU~2, Xiao-Wu FANG~1, Tian-Gang ZHUANG~1, Jie-Wen QIU~3 ~1Department of Cell Biology and Medical Genetics, Shantou University Medical College ~2Shantou University Hospital ~3Department of Infectious Disease of the Second Affiliated Hospital, Shantou University Medical College, Shantou 515031, China | 2002 | Asian Journal of Andrology2002，4，3： | 49 |
| 9 | Distant hybridization leads to different ploidy fishes显示文摘Distant hybridization makes it possible to transfer the genome of one species to another, which results in changes in phenotypes and genotypes of the progenies. This study shows that distant hybridization or the combination of this method with gynogenesis or androgenesis lead to different ploidy fishes with genetic variation, including fertile tetraploid hybrids, sterile triploid hybrids, fertile diploid hybrids, fertile diploid gynogenetic fish, and their derived progenies. The formations of the different ploidy fishes depend on the genetic relationship between the parents. In this study, several types of distant hybridization, including red crucian carp (Carassius auratus red var.) (2n=100, abbreviated as RCC) (♀)×common carp (Cyprinus carpio L.) (2n=100, abbreviated as CC) (♂), and RCC (2n=100) (♀)×blunt snout bream (Megalobrama amblycephala) (2n=48, abbreviated as BSB) (♂) are described. In the distant hybridization of RCC (♀)×CC (♂), bisexual fertile F3–F18 allotetraploid hybrids (4n=200, abbreviated as 4nAT) were formed. The diploid hybrid eggs and diploid sperm generated by the females and males of 4nAT developed into diploid gynogenetic hybrids and diploid androgenetic hybrids, respectively, by gynogenesis and androgenesis, without treatment for doubling the chromosome. Improved tetraploid hybrids and improved diploid fishes with genetic variation were derived from the gynogenetic hybrid line. The improved diploid fishes included the high-body RCC and high-body goldfish. The formation of the tetraploid hybrids was related to the occurrence of unreduced gametes generated from the diploid hybrids, which involved in premeiotic endoreduplication, endomitosis, or fusion of germ cells. The sterile triploid hybrids (3n=150) were produced on a large scale by crossing the males of tetraploid hybrids with females of diploid fish (2n=100). In another distant hybridization of RCC (♀)×BSB (♂), different ploidy fishes were obtained, including diploid bisexual fertile natural gynogenetic fish (2n=100), sterile triploid hybrids (3n=124), and bisexual fertile tetraploid hybrids (4n=148). Furthermore, two kinds of pentaploid hybrids (5n=172 and 5n=198) were formed. The biological characteristics and the mechanisms of formation of the different ploidy fish were compared and discussed at the cellular and molecular level. The results indicated distant hybridization or the combination of this method with gynogenesis or androgenesis affects the formation of different ploidy fish with genetic variation. | LIU ShaoJun Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China | 2010 | Science China(Life Sciences)2010，53，4： | 39 |
| 10 | Molecular cloning and functional characterization of a DREB1/CBF-like gene (GhDREB1L) from cotton显示文摘The transcription factors DREB1s/CBFs play important roles in the regulation of plant resistance to environmental stresses and are quite useful for generating transgenic plants tolerant to these stresses. In the present work, a cDNA encoding DREB1/CBF-like protein (GhDREB1L) from cotton was isolated, and its sequence features, DNA binding preference, and expression patterns of the transcripts were also characterized. GhDREB1L contained one conserved AP2/ERF domain and its amino acid sequence was similar to the DREB1/CBF group of the DREB family from other plants. The DNA-binding domain of GhDREB1L was successfully expressed as a fusion protein in Escherichia coli BL21 (DE3) and purified by Ni-NTA affinity chromatography. Electrophoretic mobility shift assay revealed that the purified GhDREB1L fusion protein had a specific binding activity with the previously characterized DRE ele-ment (core sequence, ACCGAC) and also with the DRE-like sequence (core sequence, GCCGAC) in the promoter of the dehydration-responsive late embryogenesis-abundant gene LEA D113. Semi-quantita- tive RT-PCR showed that GhDREB1L was induced in the cotton cotyledons by low temperature, as well as drought and NaCl treatments. These results suggested that the novel cotton GhDREB1L might play an important role in response to low temperature as well as drought and high salinity through binding to the DRE cis-element. | HUANG Bo, JIN LongGuo & LIU JinYuan Laboratory of Molecular Biology and Protein Science Laboratory of the Ministry of Education, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China | 2007 | Science China(Life Sciences)2007，50，1： | 35 |
| 11 | Characterizations and identification of the candidate gene of rice thermo-sensitive genic male sterile gene tms5 by mapping显示文摘Previous study indicated that the thermo-sensitive genic malesterile(TGMS) gene in rice was regulated by temperature.TGMS rice plays an important role in hybrid rice production,because the application of the TGMS system in two-line breeding is laborsaving,timesaving,simple,inexpensive,efficient,and eliminating the limitations of the cytoplasmic male sterility(CMS) system.'AnnongS' is the first discovered and deeply studied TGMS rice lines in China.'AnnongS-1' and 'Y58S',two derivatives of TGMS line AnnongS,were both controlled by a single recessive gene named tms5,which was genetically mapped on chromosome 2.In this study,three populations('AnnongS-1' × 'Nanjing11','Y58S' × 'Q611',and 'Y58S' × 'Guanghui122') were developed and used for the molecular fine mapping of the tms5 gene.By analyzing recombination events in the sterile individuals using a total of 125 probes covering the tms5 region,the tms5 gene was physically mapped to a 19-kb DNA fragment between two markers 4039-1 and 4039-2,which were located on the BAC clone AP004039.After the construction of the physical map between two markers 4039-1 and 4039-2,a member(ONAC023) of the NAC(NAM-ATAF-CUC-related) gene family was identified as the candidate gene of the tms5 gene. | YANG Qing-kai1,LIANG Chun-yang1,LI Jun1,JIN De-min1,AHUANG Wen2,DENG Qi-yun2,WANG Bin1(1.The State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing,100101 2. Hunan Hybrid Rice Research Center,Changsha 410125) | 2007 | 湖南农业大学学报(自然科学版)2007，33，S1： | 34 |
| 12 | A meta-analysis of oxidative stress markers in schizophrenia显示文摘Oxidative stress has been identified as a possible element in the neuropathological processes of schizophrenia(SCZ).Alteration of oxidative stress markers has been reported in SCZ studies,but with inconsistent results.To evaluate the risk of oxidative stress to schizophrenia,a meta-analysis was conducted,including five markers of oxidative stress [thiobarbituric reactive substances(TBARS),nitric oxide(NO),catalase(CAT),glutathione peroxidase(GP) and superoxide dismutase(SOD)] in SCZ patients versus healthy controls.This study showed that TBARS and NO significantly increased in SCZ,while SOD activity significantly decreased in the disorganized type of SCZ patients.No significant effect size was found for the activities of GP and CAT in SCZ patients(P>0.05).Egger’s regression test observed no significant publication bias across the oxidative stress markers,but found high heterogeneities in all the 5 markers.The subgroup analysis suggested that the ethnicity,sample size of patients and sample sources may contribute to the heterogeneity of the results for TBARS,NO and SOD.The result further demonstrated the involvement of oxidative stress in the pathophysiology of schizophrenia. | ZHANG Ming1,2,ZHAO ZhongMing3,HE Lin1,2,4 & WAN ChunLing1,2 1 Bio-X Center,Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders(Ministry of Education),Shanghai Jiao Tong University,Shanghai 200030,China 2 Institutes for Nutritional Sciences,Shanghai Institute of Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China 3 Departments of Biomedical Informatics,Psychiatry,and Cancer Biology,Vanderbilt University Medical Center,Nashville,TN 37232,USA 4 Institutes of Biomedical Sciences,Fudan University,Shanghai 200032,China | 2010 | Science China(Life Sciences)2010，53，1： | 32 |
| 13 | Targeting Gene-Virotherapy of Cancer and its prosperity显示文摘为癌症的基因和病毒的治疗显示出一些治疗学的效果,但是有真实突破的缺乏。为了达到肿瘤的完全的消除的目标,在动物的异种皮移植当模特儿,我们开发了叫的新策略指向癌症的 Gene-Virotherapy,它试图联合基因治疗和 virotherapy 的优点。这新策略独自比基因或病毒的治疗生产了更强壮的反肿瘤效果。肿瘤特定的 replicative 侵入人体气管粘膜的病菌向量,指定了为 ZD55,被侵入人体气管粘膜的病菌的 55kDa E1B 区域的删除构造。结果病毒的构造不仅由明确地指向 p53 保留类似的功能到 ONYX-015 否定肿瘤,而且允许各种各样的治疗学的基因到的插入由于最新介绍的克隆的地点形成适当 ZD55 衍生物,与原来的 ONYX-015 病毒不可行的一项任务。我们证明反肿瘤一如此的衍生物完成, ZD55-IL-24,比 ZD55 virotherapy 或 Ad-IL-24 基因治疗的是乘更多的有势力的至少 100。不过,由 ZD55-IL-24 的使用的肿瘤质量的完全的消除是仅仅在一些然而并非所有老鼠观察了,显示那一治疗学的基因不对“痊愈”足够这些老鼠。有互补或合作的效果的基因是什么时候,独立克隆进 ZD55 向量并且在联合使用了(指定了为双基因治疗策略) ,好一些的结果被获得;并且如果二合适的基因被选择,在所有老鼠完成所有异种皮移植肿瘤群众的完全的消除是可能的。更全面的研究将多半为临床的试用基于这新策略导致一个协议。最后,为癌症治疗的两倍控制指向的病毒双的基因治疗的概念,和在癌症干细胞的最近的进步的含意也被讨论。 | Xin Yuan Liu~(1,2) ~1Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,320 Yue Yang Road,Shanghai 200031,China ~2Xinyuan Institute of Medicine and Biotechnology,School of Life Science,Zhejiang Sci-Tech University,Hangzhou 310018,China | 2006 | Cell Research2006，16，11： | 32 |
| 14 | Changes in plant biomass and species composition of alpine Kobresia meadows along altitudinal gradient on the Qinghai-Tibetan Plateau显示文摘Alpine Kobresia meadows are major vegetation types on the Qinghai-Tibetan Plateau. There is growing concern over their relationships among biodiversity, productivity and environments. Despite the im-portance of species composition, species richness, the type of different growth forms, and plant bio-mass structure for Kobresia meadow ecosystems, few studies have been focused on the relationship between biomass and environmental gradient in the Kobresia meadow plant communities, particularly in relation to soil moisture and edaphic gradients. We measured the plant species composition, her-baceous litter, aboveground and belowground biomass in three Kobresia meadow plant communities in Haibei Alpine Meadow Ecosystem Research Station from 2001 to 2004. Community differences in plant species composition were reflected in biomass distribution. The total biomass showed a de-crease from 13196.96±719.69 g/m2 in the sedge-dominated K. tibetica swamp to 2869.58±147.52 g/m2 in the forb and sedge dominated K. pygmaea meadow, and to 2153.08±141.95 g/m2 in the forbs and grasses dominated K. humilis along with the increase of altitude. The vertical distribution of below-ground biomass is distinct in the three meadow communities, and the belowground biomass at the depth of 0-10 cm in K. tibetica swamp meadow was significantly higher than that in K. humilis and K. pygmaea meadows (P<0.01). The herbaceous litter in K. tibetica swamp was significantly higher than those in K. pygnaeca and K. humilis meadows. The effects of plant litter are enhanced when ground water and soil moisture levels are raised. The relative importance of litter and vegetation may vary with soil water availability. In the K. tibetica swamp, total biomass was negatively correlated to species richness (P<0.05); aboveground biomass was positively correlated to soil organic matter, soil moisture, and plant cover (P<0.05); belowground biomass was positively correlated with soil moisture (P<0.05). However, in the K. pygnaeca and K. humilis meadow communities, aboveground biomass was posi-tively correlated to soil organic matter and soil total nitrogen (P<0.05). This suggests that the distribu-tion of biomass coincided with soil moisture and edaphic gradient in alpine meadows. | WANG ChangTing1,3, CAO GuangMin1, WANG QiLan1, JING ZengChun1, DING LuMing1 & LONG RuiJun2 1 Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China 2 College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730070, China 3 Graduate University of the Chinese Academy of Sciences, Beijing 100039, China | 2008 | Science China(Life Sciences)2008，51，1： | 31 |
| 15 | The abnormal expression of retinoic acid receptor-β,p53 and Ki67 protein,n normal,premalignant and malignant esophageal tissues显示文摘AIM: Esophageal cancer remains a significant healthiproblem worldwide. It is important to investigate alterationsin expression of retinoic acid receptor-β, p53 and Ki67proteins in esophageal carcinogenesis.METHODS: To find biomarkers for early identification ofesophageal cancer, we analyzed the retinoic acid receptor-j3,p53 protein and the proliferation marker Ki67 in surgicalspecimens of normal, mildly, and severely dysplastic andmalignant esophageal tissues by in situ hybridization ofRNA and immunohistochemistry.RESULTS: RAR-β was expressed in 94.3 %(33/35) of normalmucosae, 67.8 %(19/28) of the mild, 58.1% (18/31) of thesevere ieaions and 53.2 % (116/218) of tumor samples. RAR-β mRNA was expressed in 62.7 % (42/67), 55.1% (43/78) and29.2 % (7/24) of well, moderated and poorly differentiatedSSCs. The p53 and Ki67 proteins were 5.9 % (2/34) of thenormal mucosa. P53 and Ki67 stained positively in 10.7 % (3/28) and 21.4 % (6/28) of mild dysplasia, and 51.6 %(16/31)and 58. 1% (18/31) of severely dysplasia respectively.Samples from esophageal cancer showed no higher levers ofp53 and Ki67 expression than seen in severely dysplasticlesions. There was significant difference of RAR-β、 p53 andKi67 expression between normal mucosa and dysplatictissue or esophageal cancer.CONCLUSION: Loss of RAR-β expression and acctnulation ofp53 and Ki67 proteins may serve as biomarkers for earlyidentification of esophageal cancer in the high-riskpopulations. | Min Xu Sheng-Zu Chen,Department of Nuclear Medcine,Cancer Hospital(Institute),Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing 10002,1,China Yu-Lan Jin Jun Fu Hong Huang Ping Qu Hai-Mei Tian ZhaoYang Liu Wei Zhang,Central Laboratory for Tumor Biology,Cancer Hospital(Institute),Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing 100021,China | 2002 | World Journal of Gastroenterology2002，8，2： | 34 |
| 16 | Invasive mechanism and management strategy of Bemisia tabaci(Gennadius) biotype B:Progress report of 973 Program on invasive alien species in China显示文摘Bemisia tabaci(Gennadius) biotype B,called a 'superbug',is one of the most harmful biotypes of this species complex worldwide.In this report,the invasive mechanism and management of B.tabaci biotype B,based on our 5-year studies,are presented.Six B.tabaci biotypes,B,Q,ZHJ1,ZHJ2,ZHJ3 and FJ1,have been identified in China.Biotype B dominates the other biotypes in many regions of the country.Genetic diversity in biotype B might be induced by host plant,geographical conditions,and/or insecticidal application.The activities of CarE(carboxylesterase) and GSTs(glutathione-S-transferase) in biotype B reared on cucumber and squash were greater than on other host plants,which might have increased its resistance to insecticides.The higher activities of detoxification enzymes in biotype B might be induced by the secondary metabolites in host plants.Higher adaptive ability of biotype B adults to adverse conditions might be linked to the expression of heat shock protein genes.The indigenous B.tabaci biotypes were displaced by the biotype B within 225 d.The asymmetric mating interactions and mutualism between biotype B and begomoviruses via its host plants speed up widespread invasion and displacement of other biotypes.B.tabaci biotype B displaced Trialeurodes vaporariorum(Westwood) after 4-7 generations under glasshouse conditions.Greater adaptive ability of the biotype B to adverse conditions and its rapid population increase might be the reasons of its successful displacement of T.vaporariorum.Greater ability of the biotype B to switch to different host plants may enrich its host plants,which might enable it to better compete with T.vaporariorum.Native predatory natural enemies possess greater ability to suppress B.tabaci under field conditions.The kairomones in the 3rd and 4th instars of biotype B may provide an important stimulus in host searching and location by its parasitoids.The present results provide useful information in explaining the mechanisms of genetic diversity,evolution and molecular eco-adaptation of biotype B.Furthermore,it provides a base for sustainable management of B.tabaci using biological and ecological measures. | WAN FangHao1,ZHANG GuiFen1,LIU ShuSheng2,LUO Chen3,CHU Dong4,ZHANG YouJun4,ZANG LianSheng2,JIU Min2,Lü ZhiChuang1,CUI XuHong1,ZHANG LiPing4,ZHANG Fan3,ZHANG QingWen5,LIU WanXue1,LIANG Pei5,LEI ZhongRen1 & ZHANG YongJun1 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Acadeny of Agriculture Sciences,Beijing 100094,China 2 Institute of Applied Entomology,Zhejiang University,Hangzhou 310029,China 3 Institute of Plant and Environment Protection,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100089,China 4 Institute of Vegetables and Flowers,Chinese Acadeny of Agricullare Sciences,Beijing 100081,China 5 Department of Entomology,China Agriculture University,Beijing 100094,China | 2009 | Science China(Life Sciences)2009，52，1： | 30 |
| 17 | Genetic analysis and gene mapping of a new rolled-leaf mutant in rice (Oryza sativa L.)显示文摘To understand the development of rice leaf blades,we identified a new rolled-leaf mutant,w32,from indica cultivar IR64 through EMS mutagenesis. The mutant showed a stable rolled-leaf phenotype throughout the life cycle. Two F2 populations were developed by crossing w32 to cultivar IR24 and PA64. Genetic analysis showed that the rolled-leaf phenotype was controlled by a single recessive gene. To determine the location of the gene,bulked segregant analysis was carried out using mutant and wild-type DNA pools and 1846 mutant-type F2 individuals derived from the cross w32/PA64 were genotyped to locate the gene on the short arm of chromosome 7. The rolled-leaf gene,tentatively named rl11(t),is likely a new gene as no other rolled-leaf genes have been identified near the region. By developing new SSR and InDel markers,the gene was delimited to a 52 kb region near the end of the short chromosome arm. Further fine mapping and cloning of the gene are currently underway. | SHI YongFeng1,2,CHEN Jie1,LIU WenQiang1,HUANG QiNa1,2,SHEN Bo2,LEUNG Hei3 & WU JianLi1 1 Chinese National Center for Rice Improvement/National key Laboratory for Rice Biology,China National Rice Research Institute,Hangzhou 310006,China 2 College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 310016,China 3 International Rice Research Institute,Metro Manila DAPO Box 7777,Philippines | 2009 | Science China(Life Sciences)2009，52，9： | 29 |
| 18 | Polymorphism of p16INK4a gene and rare mutation of p15INK4b gene exon2 in primary hepatocarcinoma显示文摘INTRODUCTION Hepatocellular carcinoma(HCC)is the mostcommon cause of death from cancer in China.Themechanisms of hepatocarcinogenesis are not yetknown clearly,p16INK4a gene,the multiple tumorsuppressor gene 1(MTS1),encodes P16 protein,which acts as an inhibitor by binding directly toCDK4 and CDK6 and preventing its association | Yang Qin Bo Li Yong Shu Tan Zhi Lin Sun Feng Qiong Zuo Ze Fang Sun Institute of Biochemistry and Molecular Biology,West China University of Medical Sciences,Chengdu 610041,Sichuan Province,China Department of General Surgery,The First Affiliated Hospital,West China University of Medical Sciences,Chengdu 610041,Sichuan Province,China Department of Pathology,The First Affiliated Hospital,West China University of Medical Sciences,Chengdu 610041,Sichuan Province,China | 2000 | World Journal of Gastroenterology2000，6，3： | 30 |
| 19 | Specific CEA-producing colorectal carcinoma cell killing with recombinant adenoviral vector containing cytosine deaminase gene显示文摘瞄准:到杀死 CEA 积极颜色明确地使用 E 关口 i cytosine 脱氨基酶(CD ) 的表面的癌房间自杀基因,新复制缺乏的 recombinant adenoviral 向量在在哪个 CD,基因在 CEA 倡导者下面被控制被构造并且它的在里面 vitro 细胞毒素的效果被评估。方法:梭原生质标志包含 CD 基因和 CEA 基因的规章的顺序在 293 房间紧张与侵入人体气管粘膜的病菌染色体 DNA 的右手臂被构造并且重新结合。点弄污, PCR 被用来识别积极的匾。侵入人体气管粘膜的病菌的纯化被执行与在 CsCl 步坡度和滴定极端集中与匾形成试金被测量。细胞毒素的效果是有 MTT 方法的 assayed, 5-FC 的百分之五十抑制集中(IC (50 )) 用一个曲线试穿参数被计算。表面的癌房间线,是生产 CEA,和 CEA-nonproducing Hela 房间衬里的人的颜色在 cytological 测试被使用。确定的 recombinant 侵入人体气管粘膜的病菌向量 AdCMVCD, CD 基因在 CMV 倡导者下面在被控制,被用作病毒控制。量的结果被表示为平均数的吝啬的 +/- SD。统计分析用 ANOVA 测试被执行。结果:需要的 recombinant 侵入人体气管粘膜的病菌向量被称为 AdCEACD。点弄污和 PCR 的结果证明 recombinant 侵入人体气管粘膜的病菌包含了 CEA 倡导者和 CD 基因。病毒 titer 是大约 5.0 X 10 (14 ) pfu/L (在纯化以后的 -1) 。 生产CEA Lovo 房间对 5-FC 敏感并且与 AdCEACD 和 AdCMVCD 在感染以后有一样的细胞毒素的效果(在父母 Lovo 房间,感染 100 M.O.I AdCEACD 的 Lovo 房间和感染 10 M.O.I AdCMVCD 的 Lovo 房间的 5-FC 的 IC ( 50 )价值是 >15000 , 216.5+/-38.1 和 128.8+/-25.4 micromol.L (-1), P<0.001 ,分别地),并且当侵入人体气管粘膜的病菌的 m.o.i 被提高时, 5-FC 的 cytotoxicity 因此增加了( 5-FC 的 IC ( 50 )的价值被归结为 27.9+/-4.2 micromol.L (在 1000 M.O.I AdCEACD 的-1)感染了 Lovo 房间和 24.8+/-7.1 micromol.L (在 100 M.O.I AdCMVCD 的-1)感染了 Lovo 房间, P<0.05 , P<0.01 ,分别地)。CEA-nonproducing Hela 房间没与 AdCEACD 在感染以后有效果,但是 Hela 房间与 AdCMVCD 在感染以后有细胞毒素的敏感到 5-FC (在在 10 M.O.I 感染 AdCMVCD 的父母 Hele 房间和 Hela 房间的 5-FC 的 IC (50 ) 是 >15000 和 214.5+/-31.3 micromol.L (-1), P<0.001 ) 。AdCEACD/5-FC 系统也有旁观者效果,并且当 transfected 房间的比例仅仅是 10% 时,生存能力是大约 30% 。结论:recombinant 侵入人体气管粘膜的病菌向量 AdCEACD 有房间的特性类型特定的基因交货。AdCEACD/5-FC 系统可以变得一新,为 CEA 积极的瘤的基因治疗的有势力和特定的途径,特别结肠癌。 | Li-Zong Shen Wen-Xi Wu Qiang Ding Yi-Bing Hua,Department of General Surgery,The First Affiliated Hospital of Nanjing Medical University,Nanjing,210029,Jiangsu Province,China De-Hua Xu Zhong-Cheng Zheng Xin-Yuan Liu,Shanghai Institute of Biochemistry and Cell Biology,The Chinese Academy of Sciences,Shanghai,200031,China Kun Yao,Department of Microbiology and Immunology,Nanjing Medical University,Nanjing,210029,Jiangsu Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 29 |
| 20 | Intergrin gene expression profiles of human hepatocellular carcinoma显示文摘AIM: To investigate gene expression profiles of intergringenes in hepatocellular carcinoma (HCC) through theusage of Atlas Human Cancer Array membranes, semi-quantitative reverse transcription polymerase chainreaction (RT-PCR) and Northern blot.METHODS: Hybridization of cDNA array membrane wasperformed with α 32P-labeled cDNA probes synthesizedfrom RNA isolated from hepatocellular carcinoma andadjacent non-cirrhotic liver. AtlasImage, which is asoftware specific to array, was used to analyze theresult. RT-PCR of 24 pairs specimen and Northern blotof 4 pairs specimen were used to confirm the expressionpattern of some intergrin genes identified by Atlasarrays hybridization.RESULTS: Among 588 genes spotted in membrane, 17genes were related to intergrin. Four genes were up-regulated, such as intergrin alpha8, beta1, beta7 andbeta8 in HCC. Whereas there were no genes down-regulated in HCC. RT-PCR and Northern blot analysisof intergrin beta1 gene gave results consistent withcDNA array findings.CONCLUSION: Investigation of these intergrin genesshould help to disclose the molecular mechanism of thecell adhesion, invasive and metastasis of HCC. A fewgenes are reported to have changed in HCC for the firsttime. The quick and high-throughout method of profilinggene expression by cDNA array provides us overviewof key factors that may involved in HCC, and may findthe clue of the study of HCC metastasis and moleculartargets of anti-metastasis therapy. The preciserelationship between the altered genes and HCC is amatter of further investigation. | Lian-Xin Liu Hong-Chi Jiang Wei-Hui Zhang An-Long Zhu Department of Surgery,the First Clinical College,Harbin Medical University,Harbin 150001,Heilongjiang Province,China Lian-Xin Liu Zhi-Hua Liu Jing Zhou Xiu-Qin Wang Min Wu National Laboratory of Molecular Oncology,Department of Cell Biology,Cancer Institute,Chinese Academy of Medical Science & Peking Union Medical College,Beijing 100021,China | 2002 | World Journal of Gastroenterology2002，8，4： | 29 |
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