|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Expression of germ cell nuclear factor in mouse germ cells and sperm during postnatal period显示文摘Aim: To assess the spatial and temporal expression of germ cell nuclear factor (GCNF) in male mouse germ cells during postnatal development and in sperm before and after capacitation. Methods: The indirect immunofluorescence method with anti-GCNF antiserum was used to investigate the GCNF expression in mice at day 8, 10,14, 17, 20, 28, 35, 70, and 420 after birth and in sperm before and after capacitation. Results: With the proceeding of spermatogenesis, GCNF was first detected in the nuclei of spermatogonia and a few early stage primary spermatocytes at day 8, which was increased gradually at day 10 to 14 inclusive. From day 17 to day 20, the GCNF was concentrated in round spermatids, while both spermatogonia and early stage primary spermatocytes became GCNF negative. From day 28 until day 420, strong GCNF expression was shown in round spermatids and pachytene spermatocytes, while spermatogonia, early primary spermatocytes and elongating spermatids were all GCNF negative.In addition, it was also found that GCNF was localized on the acrosomal cap region of spermatozoa and there was a big change in GCNF expression during capacitation, from 98 % GCNF positive before capacitation to about 20 % positive following capacitation. The localization of GCNF in caput and cauda spermatozoa was similar. Conclusion:GCNF may play important roles in spermatogenesis, capacitation and fertilization. (Asian J Androl 2004 Sep; 6: 217-222) | ChenXu Zong-YaoZhou Qiang-SuGuo Yi-FeiWang | 2004 | Asian Journal of Andrology2004，6，3： | 11 |
| 2 | Lyzl4, a novel mouse sperm-related protein, is involved in fertilization显示文摘鸡类型(c 类型) 溶解酵素的角色,原型溶解酵素,在免疫在许多有机体被描绘了。在这研究,我们克隆新奇 c 类型像溶解酵素的基因, Lyzl4,它位于老鼠染色体 9F4 并且与通常认为的信号肽和一个朊酶劈开地点编码了 145 氨基酸。在酵母表示的成熟 recombinant Lyzl4 蛋白质没显示出 bacteriolytic 活动。顺序排列分析证明在 c 类型溶解酵素的 20 不变的残余中的 3 个在 Lyzl4 被改变。改变的氨基酸(D52G ) 之一位于催化领域。Lyzl4 mRNA 有选择地在成年鼠标在睾丸和 epididymis 被表示,与改变越过不同发展阶段的表达式水平。Lyzl4 蛋白质的高水平在 acrosomal 区域和尾巴的主要的片的精子上被发现。在有它的特定的抗体的精子的 Lyzl4 蛋白质的免疫中立化显著地以一种剂量依赖者方式在 vitro 授精百分比减少了,建议 Lyzl4 可能为授精是重要的。 | Ruilin Sun Ruling Shen Jun Li Guojiang Xu Jun Chi Limei Li Jianke Ren Zhugang Wang Jian Fei | 2011 | Acta Biochimica et Biophysica Sinica2011，43，5： | 9 |
| 3 | 利用改良后的脾内免疫和半固体培养基法制备单克隆抗体显示文摘旨在利用改良后的脾内免疫和半固体培养基法制备单克隆抗体,节约免疫原用量、简化试验操作、一步法筛选和克隆杂交瘤细胞、缩短单抗制备周期。通过局部麻醉小鼠脾脏区域皮肤,切开后透过腹膜直接向脾脏内注射微量抗原,使小鼠的脾细胞在短时间内产生免疫反应,尾静脉注射强化免疫后进行细胞融合;选取半固体培养基(甲基纤维素、软琼脂)法,以有限稀释法作对照筛选分泌高效价抗体的杂交瘤细胞。结果显示,与传统的单抗制备方法相比,改进后的脾内免疫操作更简单,联合尾静脉注射,使用微量免疫原在短时间内产生高效价抗体;半固体培养基甲基纤维素浓度在1.1%条件下,获得克隆团较多,阳性率较高。本研究方法适用于大规模制备单克隆抗体。 | 任瑞敏 王云龙 张怡青 李恒思 王国强 李玉林 王继创 | 2013 | 生物技术通报2013，29，8： | 8 |
| 4 | 基于单克隆抗体的双酚A半抗原直接包被的酶联免疫吸附法的建立显示文摘传统的人工抗原包被酶联免疫吸附法(ELISA)依赖疏水相互作用将人工抗原与酶标板结合,会影响半抗原的呈现与识别,且多采用多克隆抗体为检测抗体,这些均会导致检测灵敏度下降和标准化难度增大。该研究选择双酚酸(BVA)作为双酚A(BPA)的半抗原与蛋白质偶联制备人工抗原免疫BALB/c小鼠,获得一株可分泌BPA单克隆抗体(MAb)的杂交瘤细胞株。利用3-氨丙基三乙氧基硅烷硅化处理酶标板,将BVA直接包被在酶标板上,建立了一种基于MAb的半抗原直接包被的BPA间接竞争ELISA法。该检测方法最低检测限IC10为0.29 ng/mL,半数抑制率IC50为5.4 ng/mL,水样中加标回收率为94.04%~102.31%。与人工抗原包被BPA ELISA检测方法(IC10:0.5 ng/mL,IC50:16.65 ng/mL,水样中加标回收率为89.72%~105.25%)相比,检测灵敏度显著性提高。 | 张玉超 刘旭东 | 2020 | 食品科技2020，45，3： | 7 |
| 5 | Spatial and temporal expression of germ cell nuclear factorin murine epididymis显示文摘Aim:To investigate the spatial and temporal expression of germ cell nuclear factor (GCNF) in mouse and rat epididymis during postnatal period.Methods:The epididymal sections from different postnatal days were stained for GCNF by the indirect immunofluorescence technique and digital photographs were taken by a Carl Zeiss confocal microscope.Results:GCNF was first detected on day 12 in mouse epididymis and day 14 in rat epididymis.The highest expression of GCNF was observed on day 35 in both mouse and rat epididymis.In adults,GCNF exhibited a region-specific expression pattern,i.e.,it was expressed predominantly in the initial segment,caput and proximal corpus of rat epididymis and was abundant in the proximal corpus of mouse epididymis.GCNF could be found in the nuclei of the principal,apical,narrow,clear and halo cells.Conclusion:GCNF may play an important role in epididymal differentiation and development and in sperm maturation.(Asian J Andro12004 Mar,6:23-28) | Zong-YaoZhou ChenXu Qiang-SuGuo Yuan-XinHu Yong-LianZhang Yi-FeiWang | 2004 | Asian Journal of Andrology2004，6，1： | 7 |
| 6 | 青鱼生长激素的重组表达及其多克隆抗体的制备(英文)显示文摘以含有的青鱼生长激素编码区cDNA的重组质粒pbcGHc为模板,高保真PCR扩增青鱼生长激素(GH)成熟肽cDNA序列,定向插入原核表达载体pET-28a,构建青鱼GH原核表达质粒pET-bcGH。将pET-bcGH转化大肠杆菌BL21(DE3),IPTG诱导青鱼GH基因在大肠杆菌中的融合表达,SDS-PAGE凝胶电泳结果显示一条23 kDa的诱导表达重组青鱼GH带。以草鱼GH多克隆抗体为一抗,Western blot证明,该重组青鱼GH具有免疫学活性。将经过亲和层析、透析纯化后的重组青鱼GH作为抗原,采用改进的方法对家兔进行皮下免疫注射,获得青鱼GH多克隆抗血清。以该多抗为一抗,Western blot可以检测出4 ng的抗原量;并且在青鱼垂体组织抽提液中和血清中检测到一种能与该抗血清作用的大小为21 kDa的蛋白质。结果表明:得到的青鱼GH多克隆抗血清具有较好的免疫特性。 | 冯浩 成嘉 刘妍 骆剑 李建中 刘少军 刘筠 | 2005 | 遗传2005，27，5： | 6 |
| 7 | 1种改良的高效单特异性兔多克隆抗体的制备方法显示文摘目的建立1种改良的高效单特异性兔多克隆抗体的制备方法。方法用RT-PCR方法获得bax保守N端1~123位氨基酸基因片段,并将其插入pET42a原核表达载体,诱导表达的Bax融合蛋白组合应用GST、His亲和层析技术获得免疫原(pET42a/Bax融合蛋白),HPLC鉴定纯度达95%。利用改良快速免疫法获得人Bax兔多克隆抗体,并经蛋白A柱亲和层析技术,抗原亲和纯化技术获得高效价高特异性的抗体。间接ELISA检测抗体滴度、Western blot和免疫组化试验检测抗体特异性,并与商业化抗体进行对比。结果通过快速免疫法得到人Bax兔多克隆抗体,经过Protein A柱纯化,再经抗原亲和纯化后,间接ELISA证明,抗体效价均达1:51 200;Western blot显示,只有经过抗原亲和纯化后的抗体特异性高,无其他杂带;免疫组化证明,在原发性肝癌组织中,人Bax兔多克隆抗体能特异地和内源性Bax结合,其高效高特异性已达国外Santa Cruse公司Bax商业化抗体水平。结论快速免疫法与抗原亲和纯化相结合,获得人Bax高效单特异性兔多克隆抗体,建立了1种改良的高效单特异性兔多克隆抗体的制备方法。 | 钟丹 易维京 李淑慧 胡川闽 | 2010 | 国际检验医学杂志2010，31，4： | 6 |
| 8 | 兔抗人APE1多克隆抗体的制备及鉴定显示文摘目的:制备兔抗人脱嘌呤/脱嘧啶核酸内切酶多克隆抗体并进行鉴定。方法:以纯化的全长APE1蛋白为抗原,采用改良的快速免疫法制备兔抗人APE1多克隆抗体并进行特异性亲和纯化,ELISA、Western blot和免疫组化检测制备抗体的效价和特异性。结果:ELISA检测显示该抗体的效价达到1∶128000,相对亲和力常数为8.96×10-6mol/L。Western blot显示该抗体能与APE1蛋白特异性结合,免疫组化检测显示阳性反应产物主要定位于细胞核。此外,该抗体还可用于小鼠和大鼠组织APE1蛋白的检测。结论:制备的多克隆抗体特异性和效价良好,不仅为今后深入研究人APE1蛋白的性质与功能提供了有用的实验工具,还可用于检测小鼠和大鼠组织中APE1蛋白的表达。 | 曹晓静 戴楠 易维京 李增鹏 杨宇馨 王东 胡川闽 | 2009 | 细胞与分子免疫学杂志2009，25，4： | 5 |
| 9 | 基于单克隆抗体的双酚A间接竞争酶联免疫分析法的建立显示文摘为建立一种简便、快速、准确的双酚A检测方法,采用活性酯法将双酚酸与牛血清蛋白、卵清蛋白偶联制备人工抗原免疫BALB/c小鼠。选择抗血清效价和灵敏度高的BALB/c小鼠,采用聚乙二醇法制备杂交瘤细胞,获得一株可分泌双酚A单克隆抗体的杂交瘤细胞株。通过方阵滴定和反应条件优化,建立一种基于单克隆抗体的双酚A间接竞争酶联免疫分析法。该检测方法在0.5 ng/mL^50 ng/mL内有良好的线性关系,最低检测限IC10为0.5 ng/mL,半数抑制率IC50为16.65 ng/mL,水样中加标回收率为89.72%~105.25%。该单克隆抗体效价高、特异性强,该检测方法灵敏度高。 | 张玉超 刘旭东 | 2020 | 食品研究与开发2020，41，17： | 5 |
| 10 | An Effective Method for Raising Antisera Against β-defensins: Double-copy Protein Expression of mBin1b in E. coli显示文摘Bin1b is a rat epididymis specific β-defensin which may have fertility related functions in addition to its antimicrobial activity. β-defensins are cysteine-rich cationic antimicrobial peptides that have their important implications in innate and adaptive immunity. Though considerable numbers of new β-defensins have been discovered, few corresponding antibodies have been reported. The small peptide with special structure and antimicrobial nature of β-defensins make them very difficult to express in prokaryotic system. Here we adopted a double-copy protein expression scheme based on which not only the mBin1b protein was successfully expressed but also the immunity of the antigen was enhanced. The validity of the antisera was verified by using Western blotting and immunohistochemical analyses. It will be a useful tool for deeply investigating the roles of Bin1b and also provide a simple but effective method in raising antisera against other members of the β-defensin gene family. | Li-Qing XIAO Ai-Hua LIU Yong-Lian ZHANG | 2004 | Acta Biochimica et Biophysica Sinica2004，36，8： | 5 |
| 11 | 人肾尿酸转运蛋白1基因克隆、抗体制备及其在肾小管上皮细胞中的定位显示文摘目的制备人尿酸转运蛋白1(hURAT1)多克隆抗体并利用该抗体检测hURAT1在肾组织中的表达及亚细胞定位。方法用RT PCR方法从人肾组织中扩增出hURAT1基因全长及其抗原表位区,分别构建绿色荧光蛋白hURAT1pEGFP融合表达载体和GST融合表达载体pGEX5X1。诱导表达并纯化GST融合蛋白,利用改良的蛋白免疫法制备多克隆抗体;Westernblot及免疫组化方法观察人肾组织的hUART1基因产物的表达。将hURAT1pEGFP重组质粒转染细胞,激光共聚焦显微镜动态观察hU RAT1pEGFP在肾小管上皮细胞LLC PK1细胞膜的定位及表达。结果成功获得高效特异的hURAT1抗体,利用该抗体证实hU RAT1基因编码产物分布于人肾组织近端肾小管刷状缘,Westernblot证实hURAT1蛋白在肾组织中表达。激光共聚焦显微镜动态观察显示hURAT1pEGFP定位于LLC PK1细胞膜。结论制备的hURAT1抗体及其全长基因可用于hURAT1基因的生理功能及病理研究,hURAT1基因编码产物分布于人肾组织近端肾小管刷状缘。 | 吴镝 张萍 陈香美 洪权 许国双 张晓洁 冯哲 丁瑞 侯剀 | 2005 | 解放军医学杂志2005，30，5： | 4 |
| 12 | Expression and localization of VCX/Y proteins and their possible involvement in regulation of ribosome assembly during spermatogenesis显示文摘Variable Charge X/Y (VCX/Y) is a human testis-specific gene family that localized on X and Y chromo-somes. In this study, VCY protein was expressed in E. coli in the form of glutathione-S-transferase (GST)fusion protein. With the purified fusion protein as antigen, the anti-GST-VCY antibody was generated andthe localization of VCY protein in human testis was determined by immunohistochemistry. In the testisseminiferous epithelium, VCY proteins were highly expressed in nuclei of germ cells. Using propidium io-dide staining and green fluorescent protein (GFP) tag technologies, VCY and VCX-8r proteins were mainlylocalized in the nucleoli of COS7 cells. In addition, the colocalization for VCY and VCX-8r in COS7 cellswas also observed. With VCY cDNA as bait, a cDNA fragment of acidic ribosomal protein PO was obtainedusing yeast two-hybrid system. All the information above indicates that VCX/Y protein family might beinvolved in the regulation of ribosome assembly during spermatogenesis. | SHENGWEIZOU JIANCHAOZHANG XIAODONGZHANG SHIYINGMIAO SHUDONGZONG QISHENG LINFANGWANG | 2003 | Cell Research2003，13，3： | 4 |
| 13 | Erosive Wear and Wear Mechanism of in situ TiC_P/Fe Composites显示文摘The base structure of in situ TiCp/Fe composites fabricated under industrial condition was changed by differentheat treatments. Erosive wear tests were carried out and the results were compared with that of wear-resistant whitecast iron. The results suggest that the wear resistance of the in situ TiCP/Fe composite is higher than that ofwear-resistant white cast iron under the sand erosive wear condition. The wear mechanism of the wear-resistantwhite cast iron was a cycle process that base surface was worn and carbides were exposed, then carbides was brokenand wear pits appeared. While the wear mechanism of in situ TiCP/Fe composite was a cycle process that basesurface was worn and TiC grains were exposed and dropped. The wear resistance of in situ TiCP/Fe composite waslower than that of wear-resistant white cast iron under the slurry erosive wear condition. Under such circumstance,the material was not only undergone erosive wear but also electrochemistry erosion due to the contact with waterin the medium. The wear behaviours can be a combination of two kinds of wear and the sand erosive wear is worsethan slurry erosive wear. | Zhaojing LIU, Zhiliang NING , Fengzhen LI, Xiurong YAO and Shanzhi RENSchool of Materials Science and Engineering, Harbin University of Science and Technology, Harbin 150080, China | 2005 | Journal of Materials Science & Technology2005，21，5： | 3 |
| 14 | 邻苯二甲酸二戊酯半抗原的合成及酶联免疫分析法的建立显示文摘邻苯二甲酸二戊酯(DAP)是一种常见的邻苯二甲酸酯类增塑剂,使用范围广泛。为构建一种DAP简便、准确的检测方法,首先进行DAP半抗原的合成,得到半抗原4-氨基邻苯二甲酸二戊酯(DPAP);采用重氮法将DPAP与蛋白质进行偶联得到DAP完全人工抗原,对免疫BALB/c小鼠进行免疫;免疫后取小鼠脾脏细胞与骨髓瘤细胞进行融合,筛选得到一株可产生DAP单克隆抗体(MAb)的杂交瘤细胞株。随后对DPA的间接竞争酶联免疫检测法进行构建,对检测条件进行优化确定最佳检测方法。所制备的DAP MAb特异性强,与DAP结构类似物产生交叉反应的反应率低。所构建的检测法最低检测限IC_(10)为0.51 ng/mL,半数抑制率IC_(50)为17.83 ng/mL,水样中加标回收率为95.36%~104.5%,可用于实际样品中DAP含量的检测,具有良好的应用前景。 | 余梅 张智淮 张玉超 吴福勇 刘旭东 | 2022 | 食品科技2022，47，7： | 3 |
| 15 | 一个新的高保守自身免疫反应相关分子IRF-4结合蛋白多克隆抗体制备显示文摘目的获得一个新的高保守自身免疫反应相关分子IRF-4结合蛋白(IRF-4-binding protein,IBP)的高效价特异性多克隆抗体。方法通过生物信息学分析选择IBP特异片段(1228~1893bp),目的片段cDNA插入pET32a和pGEX-KG原核表达载体,分别转化BL21感受态细菌,IPTG诱导表达后组合应用阴离子交换层析、His、GST亲和层析技术获得免疫原(pET32a/IBP融合蛋白)及检测原(pGEX-KG/IBP融合蛋白)。HPLC鉴定纯度。利用改良快速免疫法获得兔抗IBP多克隆抗血清,并经蛋白A柱纯化,非特异性抗体吸收。间接ELISA检测抗体滴度、Western blot和免疫组化实验检测抗体特异性。结果表达并纯化的pET32a/IBP融合蛋白纯度达92%,pGEX-KG/IBP融合蛋白纯度达87%。IBP多克隆抗体滴度达1:51200,能特异地和内源性IBP结合。结论成功表达并纯化了IBP融合蛋白,制备了高滴度、高特异性的抗IBP多克隆抗体。 | 张竹君 周玉 李鹏 易维京 杨明珍 李淑慧 胡川闽 | 2008 | 第三军医大学学报2008，30，13： | 3 |
| 16 | New member of the guanosine triphosphatase activating protein family in the human epididymis显示文摘尽管没有差距在 epididymis 被探索了,在精子发生上激活蛋白质(差距) 的海鸟粪正弦 triphosphatase 的效果多年被学习了,为精子成熟的一个必要机关。在这研究,一个新差距成员,指定了为 MacGAP,在人的 epididymis 被克隆。有 70 kDa 和港口的一种通常认为的尺寸的 618 氨基酸的 Mac 差距 geneencodesaprotein 保存 RhoGAP 领域。MacGAP 的 N 终端和 C 终端肽被表示,他们的相应的反时代被准备。对 N 终端肽的反时代能检测象 0.3 ng 一样低的抗原,并且它的特性也被证实。然而,因为它的低敏感,对 C 终端肽的反时代没能检测它的抗原。Immunohistochemistry 证明 MacGAP 蛋白质依赖于 epididymis 并且有一个区域特定的表示模式,与在上皮的房间在头区域的基础节的高表示。结果在精子成熟为差距的生物效果的进一步的解释创造了一个基础。 | Xiangqi Li Qiang Liu Shigui Liu Jinsong Zhang Yonglian Zhang | 2008 | Acta Biochimica et Biophysica Sinica2008，40，10： | 3 |
| 17 | CV-A5抗体制备及ELISA抗原检测方法的建立显示文摘目的:制备柯萨奇病毒A组5型(CV-A5)多克隆抗体和单克隆抗体,建立CV-A5抗原定量ELISA检测方法,用于CV-A5疫苗研制中抗原定量及质量控制。方法:纯化后的CV-A5全病毒颗粒作为免疫原,制备兔多克隆抗体并免疫BALB/c小鼠,采用常规细胞融合技术制备、中和试验和ELISA法筛选获得CV-A5单克隆抗体。建立CV-A5抗原检测方法,确定线性范围,验证其准确度、精密度、稳定性、特异性;检测CV-A5病毒颗粒纯化过程样品抗原含量。结果:制备了高效价的CV-A5兔多克隆抗体及单克隆抗体并建立ELISA抗原检测法,检测范围为15.61000.0 ng/ml;高、中、低3个浓度样品准确度验证回收率为88.5%128.7%;重复性验证CV分别为1.3%、3.2%、1.2%;中间精密度验证CV分别为2.1%、2.2%和3.5%;耐用性验证回收率为97.4%127.6%;包被微孔板37℃放置3 d,样品回收率为101.7%106.9%;特异性验证结果显示抗原检测方法仅识别CV-A5抗原,与CV-A5以外的抗原均无交叉反应。结论:建立的CV-A5 ELISA抗原检测法可用于纯化过程样品的抗原检测,为含CVA5的手足口病(HFMD)多价疫苗的研制提供质量控制方法。 | 张小宇 靳卫平 王文辉 吴杰 卢佳 孟胜利 王泽鋆 申硕 | 2021 | 中国免疫学杂志2021，37，11： | 2 |
| 18 | 猴ESc-615基因重组蛋白质片段的表达纯化和多克隆抗体的制备显示文摘ESc 6 15是从猕猴中克隆到的一个在附睾中特异性表达的新基因。为了从蛋白质水平深入研究其在精子成熟中的作用 ,在大肠杆菌中表达了该蛋白质的一条含 310个氨基酸的肽段 ,并用其免疫新西兰大白兔 ,得到了滴度为 10 0 0 0 0的抗血清 ;用Western印迹方法鉴定发现该抗血清可检测到 3ng的抗原量 ;并在大鼠附睾组织抽提液中检测到一种能与该抗血清作用的大小约 6 3kD的蛋白质 ,此蛋白质大小与作者实验室在大鼠中克隆到的此基因的同源蛋白质相同 ;利用该抗体通过免疫组化分析确定了ESc 6 15为分泌蛋白质 ,并能与精子结合 ;该抗血清经抗原吸附后阳性结果消失。该高滴度多克隆抗体的获得为ESc 6 15功能的探索提供了一条途径。 | 胡志红 李彬 刘强 张永莲 | 2002 | 生物化学与生物物理学报2002，34，6： | 2 |
| 19 | 奶牛S100A12蛋白多克隆抗血清的制备显示文摘为研究奶牛S100A12蛋白功能提供一种灵敏、高效的免疫学检测试剂,将纯化好的S100A12蛋白分别与弗氏完全佐剂和弗氏不完全佐剂乳化制备成抗原,免疫新西兰大白兔制备S100A12多克隆抗血清,应用琼脂双扩散法、间接ELISA法和Western blot方法检测该抗体的效价及其特异性。结果表明,所制备的S100A12抗血清经琼扩法和间接ELISA法检测效价分别达到1∶8和1∶409 600,同时免疫印迹法证实该抗体能与S100A12蛋白特异性结合。本试验成功获得了特异性强、效价高的S100A12多克隆抗体,为进一步深入研究S100A12基因功能提供了有力工具。 | 崔亚飞 姚学萍 雍康 李计尚 余树民 曹随忠 | 2012 | 动物医学进展2012，33，6： | 2 |
| 20 | 【原创论文】小鼠杀菌渗透增强性蛋白起源于睾丸及附睾表达并定位于精子中显示文摘杀菌渗透增强性蛋白(BPI)是具有抗革兰氏阴性菌活性的内源性杀菌蛋白。在本研究中,我们通过自行制备的多克隆抗体,检测了BPI蛋白在小鼠出生后睾丸及附睾组织中的表达,以及在附睾精子头部的亚细胞定位。实验结果表明,睾丸和附睾均独立表达BPI基因。在附睾中,自起始段至尾部,BPI蛋白的表达水平递减,并逐步特异性富集于亮细胞的胞质中。在顶体反应前的顶体基质内可见BPI蛋白,应起源于睾丸表达;顶体反应后,可见BPI蛋白分布于整个精子头部质膜表面,尤其是赤道板区域,可能有睾丸或附睾表达的两种起源。我们的研究结果提示,BPI蛋白可能参与顶体反应前后精子质膜结构的调控,并参与后续的精卵融合过程。 | Zhong-Ping Zhou | 2014 | Asian Journal of Andrology2014，16，2： | 2 |
您所在的机构暂未开通此部分的功能，请联系管理员