|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | The m^(6)A methylome of SARS-CoV-2 in host cells显示文摘The newly identified Severe Acute Respiratory Syndrome Coronavirus 2(SARS-CoV-2)has resulted in a global health emergency because of its rapid spread and high mortality.The molecular mechanism of interaction between host and viral genomic RNA is yet unclear.We demonstrate herein that SARS-CoV-2 genomic RNA,as well as the negative-sense RNA,is dynamically N6-methyladenosine(m^(6)A)-modified in human and monkey cells.Combined RIP-seq and miCLIP analyses identified a total of 8 m^(6)A sites at single-base resolution in the genome.Especially,epidemic strains with mutations at these identified m^(6)A sites have emerged worldwide,and formed a unique cluster in the US as indicated by phylogenetic analysis.Further functional experiments showed that m^(6)A methylation negatively regulates SARS-CoV-2 infection.SARS-CoV-2 infection also triggered a global increase in host m^(6)A methylome,exhibiting altered localization and motifs of m^(6)A methylation in mRNAs.Altogether,our results identify m^(6)A as a dynamic epitranscriptomic mark mediating the virus–host interaction. | Jun’e Liu Yan-Peng Xu Kai Li Qing Ye Hang-Yu Zhou Hanxiao Sun Xiaoyu Li Liu Yu Yong-Qiang Deng Rui-Ting Li Meng-Li Cheng Bo He Jia Zhou Xiao-Feng Li Aiping Wu Chengqi Yi Cheng-Feng Qin | 2021 | Cell Research2021，31，4： | 5 |
| 2 | Correlation of vascular endothelial growth factor to permeability of blood-brain barrier and brain edema during high-altitude exposure显示文摘BACKGROUND:Many studies have evaluated the role of vascular endothelial growth factor(VEGF) in traumatic brain edema and hemorrhagic brain edema. OBJECTIVE:To observe the effects of VEGF expression on permeability of the blood-brain barrier (BBB) during high-altitude and hypoxia exposure,and to investigate the correlation between VEGF expression and BBB permeability with regard to Evans blue staining and brain edema during high-altitude exposure. DESIGN,TIME AND SETTING:The randomized,controlled,animal study was performed at the Tanggula Etape,Central Laboratory of Chengdu Medical College,and Central Laboratory of General Hospital of Chengdu Military Area Command of Chinese PLA,China,from July 2003 to November 2004. MATERIALS:Quantitative RT-PCR kit(Sigma,USA),VEGF ELISA kit(Biosource,USA),and Evans blue(Jingchun,China) were acquired for this study. METHODS:A total of 180 Wistar rats were equally and randomly assigned to 15 groups:low-altitude (500 m),middle-altitude(2 880 m),high-altitude(4 200 m),super-high-altitude(5 000 m),1,3,5,7,9, 11,13,15,17,19,and 21 days of super high-altitude exposure.Wistar rats were exposed to various altitude gradients to establish a hypoxia model. MAIN OUTCOME MEASURES:Brain water content was calculated according to the wet-to-dry weight ratio.BBB permeability to Evans blue was determined by colorimetric method.VEGF mRNA and protein levels in brain tissues were detected using RT-PCR and double-antibody sandwich ELISA. RESULTS:Brain water content,BBB permeability to Evans blue,and VEGF mRNA and protein levels in brain tissues increased with increasing altitude and prolonged exposure to altitude.The greatest increase was determined on day 9 upon ascending 5 000 m.Simultaneously,VEGF expression positively correlated to BBB permeability of Evans blue and brain water content(r = 0.975,0.917,P<0.01). CONCLUSION:Increased VEGF protein and mRNA expression was responsible for increased BBB permeability,which may be an important mechanism underlying brain edema during high-altitude exposure. | Qiquan Zhou Chang'e Liu Jing Wang Yunli Wang Bo Zhou | 2009 | Neural Regeneration Research2009，4，10： | 4 |
| 3 | Development and calibration of the Moon-based EUV camera for Chang'e-3显示文摘The process of development and calibration for the first Moon-based extreme ultraviolet(EUV) camera to observe Earth's plasmasphere is introduced and the design, test and calibration results are presented. The EUV camera is composed of a multilayer film mirror, a thin film filter, a photon-counting imaging detector, a mechanism that can adjust the direction in two dimensions, a protective cover, an electronic unit and a thermal control unit. The center wavelength of the EUV camera is 30.2 nm with a bandwidth of 4.6 nm. The field of view is 14.7° with an angular resolution of 0.08°, and the sensitivity of the camera is 0.11 count s-1Rayleigh-1. The geometric calibration, the absolute photometric calibration and the relative photometric calibration are carried out under different temperatures before launch to obtain a matrix that can correct geometric distortion and a matrix for relative photometric correction,which are used for in-orbit correction of the images to ensure their accuracy. | Bo Chen Ke-Fei Song Zhao-Hui Li Qing-Wen Wu Qi-Liang Ni Xiao-Dong Wang Jin-Jiang Xie Shi-Jie Liu Ling-Ping He Fei He Xiao-Guang Wang Bin Chen Hong-Ji Zhang Xiao-Dong Wang Hai-Feng Wang Xin Zheng Shu-Lin E Yong-Cheng Wang Tao Yu Liang Sun Jin-Ling Wang Zhi Wang Liang Yang Qing-Long Hu Ke Qiao Zhong-Su Wang Xian-Wei Yang Hai-Ming Bao Wen-Guang Liu Zhe Li Ya Chen Yang Gao Hui Sun Wen-Chang Chen | 2014 | Research in Astronomy and Astrophysics2014，14，12： | 4 |
| 4 | Effect of endothelial progenitor cells derived from human umbilical cord blood on oxygen-induced retinopathy in mice by intravitreal transplantation显示文摘·AIM: To investigate the effect of endothelial progenitor cells(EPCs) labeled by carboxy fluorescein diacetate succinimidyl ester(CFSE) on murine oxygen-induced retinopathy(OIR) by intravitreal transplantation.·METHODS: After isolated from human umbilical cord blood mononuclear cells, EPCs were cultivated and then labeled with CFSE in vitro. C57BL/6J mice were placed to75% hyperoxia chamber from P7 to P12 to establish OIR model. At P12, OIR mice were intravitreally injected with1 μL suspension contained 2×105EPCs(EPCs group) or isometric phosphate buffered saline(PBS group). The contralateral eye of each mice received no injection(OIR group). Evans blue angiography and frozen section were examined to track the labeled cells in OIR group at P15 and P19. Using retina paraffin sections and adenosinediphos phatase staining at P12 and P19, the effect of EPCs on OIR mice was evaluated quantitatively and qualitatively.· RESULTS: The retinas from EPCs group with less non-perfusion area and fewer peripheral tufts wereobserved at P19, comparing with that from PBS or OIR group. The retinopathy in EPCs group receded earlier with less non-ganglion cells and neovascular nuclei,together with relatively regular distribution. The counts of the neovascular nuclei at P19 were reduced by 44% or45%, compared with those of OIR group or PBS group respectively. Three days after EPCs injection, a large number of EPCs appeared in the vitreous cavity and adhered to the retinal surface. While at one week, the cells gathered between the internal plexiform layer and the inner limiting membrane, and some EPCs appeared in retinal vessels.·CONCLUSION: EPCs transplantation can participate in the reparative procedure of the neovascularization in OIR. | Dan Wang Bo Zhang Hui Shi Wei Yang Ming-Chao Bi Xiang-Fu Song Chen Zhang Jian-Hui Cheng Ji-Long Hao E Song | 2016 | International Journal of Ophthalmology(English edition)2016，9，11： | 2 |
| 5 | PCOS forum: re- search in polycystic ovary syndrome today and tomorrow 显示文摘 | Pasquali R Stener-Vietorin E Yildiz BO | 2011 | Clin Endocrinol(Oxf)2011，74，4： | 1 |
| 6 | Tetradecane and hexadecane binary mixtures as phase change materials(PCMs) for cool storage in district cooling systems显示文摘 | He Bo Gustafsson E Fredrik Setterwall | 1999 | Energy1999，24，： | 1 |
| 7 | A review of the treatm ent of chronic hepatitis C virus infection in cirrhosis显示文摘 | Vezali E Aghem o A Colom bo M | 2010 | Clin Ther2010，32，13： | 1 |
| 8 | Detection of five novel GMO maize events by qualitative, multiplex PCR and fluorenscence capillary gel electrophoresis显示文摘 | Holck A Pedersen BO Heir E | 2010 | Eur Food Res Technol2010，231，： | 1 |
| 9 | Transient postoperative vascular endothelial growth factor(VEGF)-neutralisation improves graft survival in corneas with partly regressed inflammatory neovascularisation显示文摘 | Bachmann BO Luetjen-Drecoll E Bock F | 2009 | Br J Ophthalmol2009，93，8： | 1 |
| 10 | Biogeochemistry of sulfur and iron in Thioploca-colonized surface sediments in the upwelling area off central chile显示文摘 | Zopfi J Bottcher M E Jorgensen Bo B | 2008 | Geochimica et Cosmochimica Acta2008，72，： | 1 |
| 11 | Mitochondrial proliferation and paradoxical membrane depolarization during terminal differentiation and apoptosis in a human colon carcinoma cell line显示文摘 | Mancini M Anderson BO Caldwell E | 1997 | J Cell Biol1997，138，2： | 1 |
| 12 | The emission of volatile compounds during the aerobic and the combined anaerobic/aerobic composting of biowaste显示文摘 | Smet E Van Langenhoe H De Bo 1 | | 0，，08： | 1 |
| 13 | Role of dietary magnesium in cardiovascular disease prevention,insulin sensitivity and diabetes显示文摘 | Bo S Pisu E | 2008 | Curr Opin Lipidol2008，19，1： | 1 |
| 14 | Endocrine disrupts: a review of some sources, effects, and mechanisms of actions on behavior and neuroendocrine systems 显示文摘 | Frye C Bo E Calamandrei G | 2012 | J Neuroendo- crinol2012，24，1： | 1 |
| 15 | Seasonal trends of soil COE dynamics in a soil subject to freezing显示文摘 | Bo E | 2003 | Journal of Hydrology2003，276，1： | 1 |
| 16 | The balanced electromagnetic separation trans-ducer:A new bone conduction transducer显示文摘 | Hakansson Bo E V | 2003 | J Acoust Soc Am2003，113，2： | 1 |
| 17 | Anaerobic ammonium oxidation (anammox) in the marine environment 显示文摘 | Tage Dalsgaard Bo Thamdrup Donald E | 2005 | Res Microb2005，156，： | 1 |
| 18 | Targeted deletion of ROCK1 protects the heart against pressure overload by inhibiting reactive fibrosis显示文摘 | Zhang Y M Bo J Taffet G E | 2006 | FASEB J2006，20，7： | 1 |
| 19 | Commuter route optimized energy management of hybrid electric vehi- cles显示文摘 | Viktor L Lars J M Bo E | 2014 | IEEE Transactions on Intelligent Transporta- tion Systems2014，15，3： | 1 |
| 20 | Genetic epidemiological studies of preterm birth: Guidelines for research 显示文摘 | Craiq E Pennell Bo Jacobsson Scott M | 2006 | Am J Obstet Gynecol2006，，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员