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| 1 | Cryopreservation for delayed circulating tumor cell isolation is a valid strategy for prognostic association of circulating tumor cells in gastroesophageal cancer显示文摘AIM To demonstrate the feasibility of cryopreservation of peripheral blood mononuclear cells(PBMCs) for prognostic circulating tumor cell(CTC) detection in gastroesophageal cancer.METHODS Using 7.5 m L blood samples collected in EDTA tubes from patients with gastroesopheagal adenocarcinoma, CTCs were isolated by epithelial cell adhesion molecule based immunomagnetic capture using the Iso Flux platform. Paired specimens taken during the same blood draw(n = 15) were used to compare number of CTCs isolated from fresh and cryopreserved PBMCs. Blood samples were processed within 24 h to recover the PBMC fraction, with PBMCs used for fresh analysis immediately processed for CTC isolation. Cryopreservation of PBMCs lasted from 2 wk to 25.2 mo(median 14.6 mo). CTCs isolated from pre-treatment cryopreserved PBMCs(n = 43) were examined for associations with clinicopathological variables and survival outcomes.RESULTS While there was a significant trend to a decrease in CTC numbers associated with cryopreserved specimens(mean number of CTCs 34.4 vs 51.5, P = 0.04), this was predominately in samples with a total CTC count of > 50, with low CTC count samples less affected(P = 0.06). There was no significant association between the duration of cryopreservation and number of CTCs. In cryopreserved PBMCs from patient samples prior to treatment, a high CTC count(> 17) was associated with poorer overall survival(OS)(n = 43, HR = 4.4, 95%CI: 1.7-11.7, P = 0.0013). In multivariate analysis, after controlling for sex, age, stage, ECOG performance status, and primary tumor location, a high CTC count remained significantly associated with a poorer OS(HR = 3.7, 95%CI: 1.2-12.4, P = 0.03). CONCLUSION PBMC cryopreservation for delayed CTC isolation is a valid strategy to assist with sample collection, transporting and processing. | Daniel Brungs David Lynch Alison WS Luk Elahe Minaei Marie Ranson Morteza Aghmesheh Kara L Vine Martin Carolan Mouhannad Jaber Paul de Souza Therese M Becker | 2018 | World Journal of Gastroenterology2018，24，7： | 5 |
| 2 | 显示文摘 | Cvetko B F Brungs M P Burford R P | 1998 | Mater Sci1998，23，： | 2 |
| 3 | Musie therapy for reducing surgical显示文摘 | Brunges M Avigne G | 2003 | ARON J2003，78，5： | 1 |
| 4 | Music therapy for reducing surgical anxiety显示文摘 | Brunges M Avigne G | 2003 | ARONJ2003，78，5： | 1 |
| 5 | Sol-gel processing by aging and pore creator addition for porous silica antiflective coatings显示文摘 | Wongcharee K Brungs M Chaplin R | 2002 | Journal of Sol-gel Science and Technology2002，25，： | 1 |
| 6 | Particle residence time distribution (RTD) in three-phase annular bubble column reactor 显示文摘 | Pareek V K Yap Z Brungs M P Adesina A A | 2001 | Chem Eng Sci2001，56，： | 1 |
| 7 | New catalysts for the conversion of methane to synthesis gas: Molybdenum and tungsten carbide显示文摘 | CLARIDGE J B YORK A P E BRUNGS A J MARQUEZ-ALVAREZ C SLOAN J TSANG S C GREEN M L H | 1998 | J Catal1998，180，1： | 1 |
| 8 | 显示文摘 | Cvetko B F Brungs M P Burford R P | 1998 | Mater Sci1998，23，： | 1 |
| 9 | Ubersicht uber diecelast raceae inhactsstoffe chemie, chemotax onomie, biosynthese, pharmakologie显示文摘 | Kupchan S Brung M R Wagner H | 1978 | Phytochemistry1978，17，： | 1 |
| 10 | Nurses'perceptions of error communi- cation and reporting in the intensive care unit 显示文摘 | Nancy CE Brungs SM Nagy M | 2008 | Pat Safe2008，4，3： | 1 |
| 11 | Evidence-based practice to reduce central line infections显示文摘 | Render M L Brungs S Kotagal U | 2006 | Jt Comm J Qual Patient Saf2006，32，5： | 1 |
| 12 | Music therapy for reducing surgical anxiety显示文摘 | Brunges M Avigne G | 2003 | Aorn Journal2003，78，5： | 1 |
| 13 | Preparation and evaluation of electrocatalytic oxide coatings on conductive carbon-polymer composite substrate for use as dimensionally stable anodes 显示文摘 | BRUNGS A HADDADI-ASL V SKYLLAS-KAZACOS M | 1996 | J Appl Electrochem1996，26，： | 1 |
| 14 | Sol-gel Processing by Aging and Pore Creator Addition for Porous Silica Antireflective Coatings显示文摘 | WONGCHAREE K BRUNGS M CHAPLIN R | | 0，，： | 1 |
| 15 | Music therapy for reducing surgical anxiety显示文摘 | Brunges M Avigne G | 2003 | ARON J2003，78，5： | 1 |
| 16 | Some solutions to the nickel sulphide problem in toughened glass显示文摘 | BRUNGS M P SUGENG X Y | 1995 | Glass Technol1995，36，4： | 1 |
| 17 | Music therapy for reducing surgical anxiety显示文摘 | Brunges M Avigne G | 2003 | AORN Journal2003，78，5： | 1 |
| 18 | Degradation of sucrose and nitrate over titania coated nano-hematite photocatalysts 显示文摘 | PENPOLCHAROEN M AMAL R BRUNGS M | 2001 | J Nanoparticle Res2001，3，4： | 1 |
| 19 | Deactivation of molten salt baths used for the ion exchangestrengthening of glasses显示文摘 | RANKIN W BRUNGS M P | 1979 | Glass Technol1979，20，2： | 1 |
| 20 | Acute and long-term accumulation of copper by the brown bullhead Ictalurus nebulosus 显示文摘 | Brungs A Leonard E N McKim J M | 1973 | J Fish Res Bd Can1973，30，： | 1 |
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