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| 1 | CYP24A1 inhibition facilitates the anti-tumor effect of vitamin D3 on colorectal cancer cells显示文摘AIM:The effects of vitamin D3 have been investigated on various tumors, including colorectal cancer (CRC). 25-hydroxyvitamin-D3-24-hydroxylase (CYP24A1), the enzyme that inactivates the active vitamin D3 metabolite 1,25-dihydroxyvitamin D3 (1,25-D3), is considered to be the main enzyme determining the biological halflife of 1,25-D3. During colorectal carcinogenesis, the expression and concentration of CYP24A1 increases significantly, suggesting that this phenomenon could be responsible for the proposed efficacy of 1,25-D3 in the treatment of CRC. The aim of this study was to investigate the anti-tumor effects of vitamin D3 on the human CRC cell line Caco-2 after inhibition of the cytochrome P450 component of CYP24A1 activity. METHODS:We examined the expression of CYP24A1 mRNA and the effects of 1,25-D3 on the cell line Caco-2 after inhibition of CYP24A1. Cell viability and proliferation were determined by means of sulforhodamine-B staining and bromodeoxyuridine incorporation, respectively, while cytotoxicity was estimated via the lactate dehydrogenase content of the cell culture supernatant. CYP24A1 expression was measured by realtime reverse transcription polymerase chain reaction. A number of tetralone compounds were synthesized to investigate their CP24A1 inhibitory activity. RESULTS:In response to 1,25-D3, CYP24A1 mRNA expression was enhanced significantly, in a time- and dose-dependent manner. Caco-2 cell viability and proliferation were not influenced by the administration of 1,25-D3 alone, but were markedly reduced by coadministration of 1,25-D3 and KD-35, a CYP24A1-inhibiting tetralone. Our data suggest that the mechanism of action of co-administered KD-35 and 1,25-D3 does not involve a direct cytotoxic effect, but rather the inhibition of cell proliferation. CONCLUSION:These findings demonstrate that the selective inhibition of CYP24A1 by compounds such as KD-35 may be a new approach for enhancement of the anti-tumor effect of 1,25-D3 on CRC. | János P Kósa Péter Horváth János Wlfling Dóra Kovács Bernadett Balla Péter Mátyus Evelin Horváth Gábor Speer István Takács Zsolt Nagy Henrik Horváth Péter Lakatos | 2013 | World Journal of Gastroenterology2013，19，17： | 5 |
| 2 | Postoperative adhesion prevention using a statin-containing cellulose film in an experimental model显示文摘 | Lalountas M Ballas K D Michalakis A | | 0，，： | 1 |
| 3 | Serum levels of platelet released CIM0 ligand are increased in early onset occlusive carotid artery disease 显示文摘 | Balla J Magyar MT Bereezki D | 2006 | Dis Markers2006，22，3： | 1 |
| 4 | Serum levels of platelet released CD40 ligand are increased in early onset occlusive carotid artery disease 显示文摘 | Balla J Magyar MT Bereczki D | 2006 | Dis Markers2006，22，3： | 1 |
| 5 | Building a Spatial Microsimulation- based Planning Support System for Local Policy Making显示文摘 | Ballas D Kingston R Stillwell J | | Environment and Planning A0，39，10： | 1 |
| 6 | Online gamma-ray spectrometry at open sea显示文摘 | Tsabaris C Ballas D | 2005 | Applied Radiation and Isotopes2005，62，1： | 1 |
| 7 | Classification of Slovak white wines using artificial neural networks and discriminant techniques显示文摘 | KRUZLICOVA D MOCAK J BALLA B | 2009 | Food Chemistry2009，112，4： | 1 |
| 8 | Is more ever too much The number of indicators per factor in confirmatory factor analysis 显示文摘 | Marsh H W Har K T Balla J R Grayson D | 1998 | Multivarial Behavioral Research1998，33，2： | 1 |
| 9 | Altered wound healing in mice lacking a functional osteopontin gene (spp1)显示文摘 | LIAW L BIRK D E BALLAS C B | 1998 | J Clin Invest1998，101，7： | 1 |
| 10 | Geochemical background heavy metal concentrations of stream sediments at mineralized areas ofNE Chalkidiki 显示文摘 | KELEPERTZIS E ARGVRAKI A DAFTSIS E BALLAS D | 2010 | Hellenic Journal of Geosciences2010，45，： | 1 |
| 11 | Carbonyl com- pounds and dissolved organic carbon in rainwater of an ur- ban atmosphere 显示文摘 | Balla D Papageorgiou A Voutsa D | 2014 | Environmental Science and Pollution Research2014，21，12： | 1 |
| 12 | Business at the Margins? Business Interests in Edge Urban Politics显示文摘 | Nick Phelps Parsons N Ballas D Dowling A | 2006 | International Journal of Urban and Regional Research2006，30，2： | 1 |
| 13 | Delineation of a CpG phosphorothioate oligodeoxynucleotide for activating primate immune responses in vitro and in vivo显示文摘 | Hartmann G Weeratna R D Ballas Z K | 2000 | J Immunol2000，164，3： | 1 |
| 14 | Pathology of the optic nerve injury显示文摘 | Balla L Ianovici N Costin D | | 0，，4： | 1 |
| 15 | Induction of NK activity in murine and human cells by CpG motifs in oligodeoxynucleotides and bacterial DNA显示文摘 | Ballas Z D Rasmussen W L Krieg A M | 1996 | J Immunol1996，157，： | 1 |
| 16 | Oligodeoxynucleotide CpG 7909 delivered as intravenous infusion demonstrates immunologic modulation in patients with previously treated non-Hodgkin lymphoma显示文摘 | LINK B K BALLAS Z K WEISDORF D | 2006 | J Immunother2006，29，5： | 1 |
| 17 | Delineation of a CpG phosphorothioate oligodeoxynucleotide for activating primate immune responses in vitro and in vivo显示文摘 | Hartmann G Weeratna R D Ballas Z K | 2000 | J Immunol2000，164，： | 1 |
| 18 | Deli- neation of a CpG phosphorothioate oligodeoxy-nucleotide for activating primate immune responses in vitro and in vivo 显示文摘 | Hartmann G Weeratna R D Ballas Z K | 2000 | Immunology2000，164，： | 1 |
| 19 | Pathology of the optic nerve injury显示文摘 | Balla L Ianovici N Costin D | 2012 | Rev Med Chir Soc Med Nat Iasi2012，116，4： | 1 |
| 20 | REST and its corepressors mediate plasticity of neuronal gene chromatin throughout neurogenesis显示文摘 | BALLAS N GRUNSEICH C LU D D | 2005 | Cell2005，121，4： | 1 |
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