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| 1 | Long-term propagation of tree shrew spermatogonial stem cells in culture and successful generation of transgenic offspring显示文摘树鼯有一种靠近的关系到首领并且在生物医学的研究比啮齿类动物有许多优点。然而,基因操作方法的缺乏妨碍了这个动物的更宽的使用。Spermatogonial 干细胞(SSC ) 成功地在文化被扩展了允许复杂基因在老鼠和老鼠编辑。这里,没有干细胞性质的损失,我们为树鼯 SSC 的长期的扩大描述一个文化系统。在我们的学习,胸腺房间抗原 1 被用来充实树鼯 SSC。定序 RNA 分析表明表明小径的 Wnt/-catenin 在无差别的 SSC 是活跃的,但是是在 SSC 区别的开始之上的 downregulated。树鼯的暴露到 recombinant Wnt3a 蛋白质的主要 SSC 在文化的起始的段落期间提高了 SSC 的幸存。当 feeder 被发现为树鼯 SSC 增长必要,树鼯 Sertoli 房间,然而并非老鼠使用胚胎的成纤维细胞,导致柔韧的房间扩大和长期的文化。扩展的树鼯 SSC 是有提高的绿色的 transfected 荧光灯表示 lentiviral 向量的蛋白质(EGFP ) 。在进消毒的成年的男树鼯睾丸的移植以后,标注 EGFP 的 SSC 能恢复精子发生并且成功地产生转基因的后代。而且,这些 SSC 对 CRISPR/Cas9-mediated 基因修正合适。在有编辑的基因的联合的树鼯 SSC 接近膨胀的一个文化系统的发展用树鼯为精确染色体操作铺平道路。 | Chao-Hui Li Lan-Zhen Yan Wen-Zan Ban Qiu TH Yong Wu Lin Wang Rui Bi Shuang Ji Yu-Hua Ma Wen-Hui Nie Long-Bao Lv Yong-Gang Yao Xu-Dong Zhao Ping Zheng | 2017 | Cell Research2017，27，2： | 22 |
| 2 | Alteration of p53 and p21 during hepatocarcinogenesis in tree shrews显示文摘AIM: To investigate p53 mutation and p21 expression in hepatocarcinogenesis induced by hepatitis B virus (HBV) and aflatoxin B1 (AFB1) in tree shrews, and to reveal the role of these genes in hepatocarcinogenesis.METHODS: Tree shrews were divided into four groups:group A, those infected with HBV and fed with AFB1 (n = 39);group B, those infected with HBV alone (n = 28); group C,those fed with AFB1 alone (n = 29); and group D, normal controls (n = 20). The tree shrews underwent liver biopsies once every 15 wk. Expression of p53 and p21 proteins and genes in the biopsies and tumor tissues of the experimental tree shrews was detected, respectively, by immunohistochemistry,and by Southem blotting and reverse transcription-polymerase chain reaction and sequencing.RESULTS: The incidence of hepatocellular carcinomas (HCC) was higher in group A (66.7%) than that in group B (3.57%) and C (30%). The time of HCC occurrence was also earlier in group A than that in group C (120.0±16.6 wk vs 153.3±5.8 wk, respectively, P<0.01). p53 protein was not detected by immunohistochemistry in all groups before the 75^th wk of the experiment. At the 105^th wk, the positive rates fo p53 were 78.6%, 60% and 71.4% in groups A, B and C, respectively, which were significantly higher than that in group D (10%) (all P<0.05). An abnormal band of p53 gene was observed in groups A and C. The mutation points of p53gene in tree shrews with HCC were at codons 275, 78 and 13. The nucleotide sequence and amino acid sequence of tree shrew's wild-type p53 showed 91.7% and 93.4% homologies with those of human p53,respectively. The immunopositivity for p21 was found before HCC development. The incidence of HCC was significantly higher in tree shrews that were positive for p21 than those negative for p21 (80.0% vs 11.0%, P<0.001).The incidence of HCC in p21 positive animals in group A was significantly higher than those positive for p21 in group C (P | Jian-Jia Su Yuan Li Ke-Chen Ban Liu-Liang Qin Chun Yang Chao Ou Xiao-Xian Duan Hui-Yun Wang Rui-Qi Yang Young-Lk Lee | 2004 | World Journal of Gastroenterology2004，10，24： | 21 |
| 3 | Chinese physicians' perceptions of fecal microbiota transplantation显示文摘AIM: To explore Chinese physicians' perceptions towards fecal microbiota transplantation(FMT) and to provide information and an assessment of FMT development in China.METHODS: A self-administered questionnaire was developed according to the FMT practice guidelines and was distributed to physicians in hospitals via Internet Research Electronic Data Capture(REDcap) software and electronic mails to assess their attitudes toward and knowledge of FMT. The questionnaire included a brief introduction of FMT that was followed by 20 questions. The participants were required to respond voluntarily, under the condition of anonymity and without compensation. Except for the fill-in-the-blank questions, all of the other questions were required in the REDcap data collection systems, and the emailed questionnaires were completed based on eligibility.RESULTS: Up to December 9, 2014, 844 eligible questionnaires were received out of the 980 distributed questionnaires, with a response rate of 86.1%. Among the participants, 87.3% were from tertiary hospitals, and there were 647(76.7%) gastroenterologists and 197(23.3%) physicians in other departments(nongastroenterologists). Gastroenterologists' awareness of FMT prior to the survey was much higher than non-gastroenterologists'(54.3 vs 16.5%, P < 0.001); however, acceptance of FMT was not statistically different(92.4 vs 87.1%, P = 0.1603). Major concerns of FMT included the following: acceptability to patients(79.2%), absence of guidelines(56.9%), and administration and ethics(46.5%). On the basis of understanding, the FMT indications preferred byphysicians were recurrent Clostridium difficile infection(86.7%), inflammatory bowel disease combined with Clostridium difficile infection(78.6%), refractory ulcerative colitis(70.9%), ulcerative colitis(65.4%), Crohn's disease(59.4%), chronic constipation(43.7%), irritable bowel syndrome(39.1%), obesity(28.1%) and type 2 diabetes(23.9%). For donor selection, the majority of physicians preferred individuals with a similar gut flora environment to the recipients. 76.6% of physicians chose lower gastrointestinal tract as the administration approach. 69.2% of physicians considered FMT a safe treatment. CONCLUSION: Chinese physicians have awareness and a high acceptance of FMT, especially gastroenterologists, which provides the grounds and conditions for the development of this novel treatment in China. Physicians' greatest concerns were patient acceptability and absence of guidelines. | Rong-Rong Ren Gang Sun Yun-Sheng Yang Li-Hua Peng Shu-Fang Wang Xiao-Hong Shi Jing-Quan Zhao Yong-Ling Ban Fei Pan Xue-Hong Wang Wei Lu Jian-Lin Ren Ying Song Jiang-Bin Wang Qi-Ming Lu Wen-Yuan Bai Xiao-Ping Wu Zi-Kai Wang Xiao-Mei Zhang Ye Chen | 2016 | World Journal of Gastroenterology2016，22，19： | 12 |
| 4 | Genome-wide identification and characterization of HSP gene superfamily in whitefly (Bemisia tabacl)and expression profiling analysis under temperature stress显示文摘Heat shock proteins (HSP)are essential molecular chaperones that play important roles in the stress stimulation of insects.Bemisia tabaci,a phloem feeder and invasive species,can cause extensive crop damage through direct feeding and transmission of plant viruses.Here we employed comprehensive genomics approaches to identity HSP superfamily members in the Middle East Asia Minor 1 whitefly genome.In total,we identified 26 Hsp genes,including three Hsp90,17 Hsp70,one Hsp60 and five sHSP (small heat shock protein)genes.The HSP gene superfamily of whitefly is expanded compared with the other five insects surveyed here.The gene structures among the same families are relatively conserved.Meanwhile,the motif compositions and secondary structures of BtHsp proteins were predicted.In addition,quantitative polymerase chain reaction analysis showed that the expression patterns of BtHsp gene superfamily were diverse across different tissues of whiteflies.Most Hsp genes were induced or repressed by thermal stress (40℃)and cold treatment (4℃)in whitefly.Silencing the expression of BtHsp70-6 significantly decreased the survival rate of whitefly under 45℃.All the results showed the Hsps conferred thermo-tolerance or cold-tolerance to whiteflies that protect them from being affected by detrimental temperature conditions.Our observations highlighted the molecular evolutionary properties and the response mechanism to temperature assaults of Hsp genes in whitefly. | Xin-Ru Wang Chao Wang Fei-Xue Ban Dan-Tong Zhu Shu-Sheng Liu Xiao-Wei Wang | 2019 | Insect Science2019，26，1： | 11 |
| 5 | The construction of a genetic linkage map of non-heading Chinese cabbage (Brassica campestris ssp.chinensis Makino)显示文摘Non-heading Chinese cabbage(Brassica campestris ssp.chinensis Makino) is one of the most important vegetables in eastern China.A genetic linkage map was constructed using 127 doubled haploid(DH) lines,and the DH population was derived from a commercial hybrid 'Hanxiao'(lines SW-13 × L-118).Out of the 614 polymorphic markers,43.49% were not assigned to any of the linkage groups(LGs).Chi-square tests showed that 42.67% markers were distorted from expected Mendelian segregation ratios,and the direction of distorted segregation was mainly toward the paternal parent L-118.After sequentially removing the markers that had an interval distance smaller than 1 cM from the upper marker,the overall quality of the linkage map was increased.Two hundred and sixty-eight molecular markers were mapped into 10 LGs,which were anchored to the corresponding chromosome of the B.rapa reference map based on common simple sequence repeat(SSR) markers.The map covers 973.38 cM of the genome and the average interval distance between markers was 3.63 cM.The number of markers on each LG ranged from 18(R08) to 64(R07),with an average interval distance within a single LG from 1.70 cM(R07) to 6.71 cM(R06).Among these mapped markers,169 were sequence-related amplified polymorphism(SRAP) mo-lecular markers,50 were SSR markers and 49 were random amplification polymorphic DNA(RAPD) markers.With further saturation to the LG,the current map offers a genetic tool for loci analysis for important agronomic traits. | Yan Cheng Jianfeng Geng Jingyi Zhang Qian Wang Qingyu Ban Xilin Hou | 2009 | Journal of Genetics and Genomics2009，36，8： | 9 |
| 6 | Clinical features and outcomes of 210 patients with idiopathic pulmonary fibrosis显示文摘 | Cai Miaotian Zhu Min Ban Chengjun Su Jin Ye Qiao Liu Yan Zhao Wen Wang Chen Dai Huaping | 2014 | Chinese Medical Journal2014，，10： | 8 |
| 7 | Incidence and Clinical Characteristics of Pulmonary Hypertension in Patients with Idiopathic Pulmonary Fibrosis显示文摘 | Wei Yan Li-Ying Peng Cheng-Jun Ban Xue-Feng Xu Min Zhu Yan Liu Shu Zhang Zhen-Guo Zhai Chen Wang Hua-Ping Dai | 2015 | Chinese Medical Journal2015，，7： | 8 |
| 8 | Pneumonia relevant to lung transplantation and pathogen distribution显示文摘 | HE Xuan DAI Hua-ping CHEN Qi-rui MIAO Jin-bai SUN Bing BAO Na HU Bin LI Hui WU An-shi BAN Cheng-jun GE Su-juan WANG Chen HOU Sheng-cai | 2013 | Chinese Medical Journal2013，，17： | 7 |
| 9 | An overview of the resources and environment conditions and major geological problems in the Yangtze River economic zone, China显示文摘Focusing on the Yangtze River economic zone,the previous geological researches are systematically summarized,resources and environment conditions and major geological problems which are needing to be concerned in land planning and construction are studied.The results show that the resource conditions of cultivated land,shale gas,geotherm,lithium and so on are superior in the Yangtze River economic zone,and the resources and environment conditions are conducive to develop the modern agriculture,clean energy industry and strategic emerging industries.3×1013 m^2 farmlands without heavy metal pollution are concentrated;there are three national level shale gas exploration and development bases with explored reserves of 5.441×1011 m^3;geothermal availability is 2.4×109 t of standard coal each year,equivalent to 19% of the amount of coal in 2014;Asia's largest energy lithium metal ore deposit is found.In some parts of Yangtze River economic zone,there are some major geological problems such as active faults,karst collapse,ground subsidence,landslide-collapse-debris flow,affecting the river-crossing channels,high-speed railway,urban agglomeration and green ecological corridor planning and construction.Those problems should be concerned,and the relevant suggestions and countermeasures are put forward.Meanwhile,the ideas to further support the development of the Yangtze River economic zone are put forward. | Yue-hua Jiang Liang-jun Lin Li-de Chen Hua-yong Ni Wei-ya Ge Hang-xin Cheng Gang-yi Zhai Gui-ling Wang Yi-zhong Ban Yuan Li Ming-tang Lei Cheng-xuan Tan Jing-wen Su Quan-ping Zhou Tai-li Zhang Yun Li Hong-ying Liu Ke Peng Han-mei Wang | 2018 | China Geology2018，1，3： | 6 |
| 10 | Face Masks in the New COVID-19 Normal: Materials, Testing,and Perspectives显示文摘The increasing prevalence of infectious diseases in recent decades has posed a serious threat to public health.Routes of transmission differ,but the respiratory droplet or airborne route has the greatest potential to disrupt social intercourse,while being amenable to prevention by the humble facemask.Different types of masks give different levels of protection to the user.The ongoing COVID-19 pandemic has even resulted in a global shortage of face masks and the raw materials that go into them,driving individuals to self-produce masks from household items.At the same time,research has been accelerated towards improving the quality and performance of face masks,e.g.,by introducing properties such as antimicrobial activity and superhydrophobicity.This review will cover mask-wearing from the public health perspective,the technical details of commercial and home-made masks,and recent advances in mask engineering,disinfection,and materials and discuss the sustainability of mask-wearing and mask production into the future. | Ming Hui Chua Weiren Cheng Shermin Simin Goh Junhua Kong Bing Li Jason Y.CLim Lu Mao Suxi Wang Kun Xue Le Yang Enyi Ye Kangyi Zhang Wun Chet Davy Cheong Beng Hoon Tan Zibiao Li Ban Hock Tan Xian Jun Loh | 2020 | Research2020，，1： | 5 |
| 11 | Fatigue driving detection based on Haar feature and extreme learning machine显示文摘As the significant branch of intelligent vehicle networking technology, the intelligent fatigue driving detection technology has been introduced into the paper in order to recognize the fatigue state of the vehicle driver and avoid the traffic accident. The disadvantages of the traditional fatigue driving detection method have been pointed out when we study on the traditional eye tracking technology and traditional artificial neural networks. On the basis of the image topological analysis technology, Haar like features and extreme learning machine algorithm, a new detection method of the intelligent fatigue driving has been proposed in the paper. Besides, the detailed algorithm and realization scheme of the intelligent fatigue driving detection have been put forward as well. Finally, by comparing the results of the simulation experiments, the new method has been verified to have a better robustness, efficiency and accuracy in monitoring and tracking the drivers' fatigue driving by using the human eye tracking technology. | Chang Zheng Ban Xiaojuan Wang Yu | 2016 | The Journal of China Universities of Posts and Telecommunications2016，23，4： | 5 |
| 12 | Optimal subreflector position determination of shaped dual-reflector antennas based on the parameters iteration approach显示文摘A new method based on the parameters iteration technique has been developed to determine the optimal subreflector position for shaped Cassegrain antennas, that are distorted by gravity, to improve their electromagnetic(EM) performance. Both the features of shaped surface and the relationship between optical path difference(OPD) and far field beam pattern are employed. By describing the shaped dualreflector surface as a standard discrete parabola set, we can utilize the optical features of the standard Cassegrain system in the classical OPD relationship. Then, the actual far field beam pattern is expressed as the synthesis of ideal beam and error beam by decomposing subreflector adjustment parameters using a mechanical-electromagnetic-field-coupling-model(MEFCM). Furthermore, a numerical method for determining optimal subreflector position is presented. The proposed method is based on the iteration technique of subreflector adjustment parameters, and the optimal far field pattern is used for the iteration. The numerical solution of optimal adjustment parameters can be obtained rapidly. Results for a 25 m shaped Cassegrain antenna demonstrate that the adjustment of the subreflector to the optimal position as determined by the proposed method can improve the EM performance effectively. | Bin-Bin Xiang Cong-Si Wang Pei-Yuan Lian Na Wang You Ban | 2019 | Research in Astronomy and Astrophysics2019，19，4： | 4 |
| 13 | Silicon lithium-ion battery anode with enhanced performance: Multiple effects of silver nanoparticles显示文摘Silicon has been regarded as one of the most promising next generation lithium-ion battery anode. However, the poor cyclic stability of the Si based anode has severely limited its practical applications, which is even worse with high mass loading density(>1 mg cm^(-2)). A new concept has been developed to enhance the electrochemical performance of the Si nanoparticle anode. Silver nanoparticles are composited with the silicon nanoparticles in a facile way for the first time. It is found that the mechanical properties of the Si electrode have been significantly improved by the incorporation of the silver nanoparticles, leading to enhanced cyclic performance. With the Si/Ag mass ratio of 4:1, the reversible specific discharge capacity is retained as 1156 m A h g^(-1) after 100 cycles at 200 mA g^(-1), which is more than three times higher than that of the bare silicon(318 mA h g^(-1)). The rate performance has been effectively improved as well due to excellent electron conductivity of the silver nanoparticles. | Shanshan Yin Qing Jia Xiuxia Zuo Shuang Xie Kai Fang Yonggao Xia Jinlong Li Bao Qiu Meimei Wang Jianzhen Ban Xiaoyan Wang Yi Zhang Ying Xiao Luyao Zheng Suzhe Liang Zhaoping Liu Cundong Wang Ya-Jun Cheng | 2018 | Journal of Materials Science & Technology2018，34，10： | 4 |
| 14 | A Quantized Kernel Least Mean Square Scheme with Entropy-Guided Learning for Intelligent Data Analysis显示文摘Quantized kernel least mean square(QKLMS) algorithm is an effective nonlinear adaptive online learning algorithm with good performance in constraining the growth of network size through the use of quantization for input space. It can serve as a powerful tool to perform complex computing for network service and application. With the purpose of compressing the input to further improve learning performance, this article proposes a novel QKLMS with entropy-guided learning, called EQ-KLMS. Under the consecutive square entropy learning framework, the basic idea of entropy-guided learning technique is to measure the uncertainty of the input vectors used for QKLMS, and delete those data with larger uncertainty, which are insignificant or easy to cause learning errors. Then, the dataset is compressed. Consequently, by using square entropy, the learning performance of proposed EQ-KLMS is improved with high precision and low computational cost. The proposed EQ-KLMS is validated using a weather-related dataset, and the results demonstrate the desirable performance of our scheme. | Xiong Luo Jing Deng Ji Liu Weiping Wang Xiaojuan Ban Jenq-Haur Wang | 2017 | China Communications2017，14，7： | 4 |
| 15 | Surface Tension Model Based on Implicit Incompressible Smoothed Particle Hydrodynamics for Fluid Simulation显示文摘为了捕获液体的稳定、现实主义的显微镜的特征,基于含蓄的不可压缩的 SPH (弄平的粒子水动力学) 的表面,表面紧张和粘附方法在这份报纸被介绍。它给一个稳定、快的紧张模型和罐头解决不考虑的问题粘附。分子的结合和表面最小化为表面紧张被考虑,并且粘附被增加显示出表面的显微镜的特征。稳定地并且高效地模仿表面紧张和粘附,表面紧张和粘附模型对一个含蓄的不可压缩的 SPH 方法综合。试验性的结果证明方法罐头更好与以前的方法相比在许多情形模仿表面特征并且同时保证稳定性和效率。 | Xiao-Kun Wang Xiao-Juan Ban Ya-Lan Zhang Si-Nuo Liu Peng-Fei Ye | 2017 | Journal of Computer Science & Technology2017，32，6： | 4 |
| 16 | Electrochemical Deposition and Nucleation of Aluminum on Tungsten in Aluminum Chloride-Sodium Chloride Melts显示文摘Electrochemical deposition and nucleation of aluminum on tungsten electrode from AlCl_3-NaCl melts were studied by cyclic voltammetry,chronopotentiometry and chronoamperometry.Cyclic voltammetry and chronopo- tentiometry analyses showed that Al(Ⅲ) was reduced at 200℃in two consecutive steps in an electrolyte of molten AlCl_3-NaCl system with a composition 52:48 molar ratio.The current-time characteristics of nucle- ation aluminum on tungsten showed a strong dependence on overpotentials.Chronoamperometry showed that the deposition process of aluminum on tungsten was controlled by an instantaneous nucleation with a hemispherical diffusion-controlled growth mechanism.The results could lead to a better understanding of the AlCl_3-NaCl melt system that has technological importance in electrodeposition of metals as well as in rechargeable batteries. | Zhaowen WANG Hongmin KAN Zhongning SHI Bingliang GAO Yungang BAN Xianwei HU | 2008 | Journal of Materials Science & Technology2008，24，6： | 4 |
| 17 | Effect of Rolling Process on Microstructure and Properties of 95CrMo Drill Steel显示文摘In order to improve the strength and toughness of 95CrMo steel and explore a short flow process,a direct rolling process was employed,and the effect of finishing rolling process on microstructure and mechanical properties was investigated.The results show that,with the decrease of finishing rolling temperature,inter-lamellar spacing increases,the strength as well as hardness has a general increase,and secondary cementite is distributed uniformly but represents a remarkable decrease in size.Based on Hall-Petch type equation,an effective relationship of yield strength and pearlite structure parameters was established.The correlation coefficients between the measured and calculated strength were more than 0.95,which indicated a high reliability of the relationship.By analyzing the individual strength contributions of pearlite structures,yield strength was found to have a more great dependence on pearlite inter-lamellar spacing than colony size. | Wei YU Bao-sheng XIE Ban WANG Qing-wu CAI Shi-xin XU | 2016 | Journal of Iron and Steel Research(International)2016，23，9： | 3 |
| 18 | Monitoring the Effective Sterilization of Low-Temperature Hydrogen Peroxide Gas Plasma Sterilizers in 58 Hospitals——22 PLADs,China,June 2015-December 2019显示文摘Summary What is already known on this topic?Hydrogen peroxide sterilizeation is widely used for luminal devices.However,the low penetrability of the sterilant is of major concern.What is added by this report?This report investigated the effective sterilization of low-temperature hydrogen peroxide gas plasma sterilizers and compared the applicability of different biological monitoring methods based on medical luminal devices. | Jiaqi Wang Baoying Zhang Huihui Sun Jian Zhang Hongyang Duan Haiqun Ban Jin Shen Liubo Zhang | 2021 | China CDC weekly2021，3，29： | 3 |
| 19 | Effect of Ball Scribing on Magnetic Barkhausen Noise of Grain-oriented Electrical Steel显示文摘Effect of ball scribing on magnetic Barkhausen noise(MBN) of conventional grain-oriented(CGO) and highpermeability grain-oriented(HGO) electrical steel was investigated.The results showed that after ball scribing, root mean square of MBN(MBNrms) of CGO electrical steel increased 9.8%with 4 mm scribing spacing at 1.2 T,and that of HGO electrical steel apparently decreased 17.3%with 16 mm scribing spacing at 1.2 T. Through the formation and development of free magnetic poles and secondary magnetic domains due to compressive stress,primary magnetic domain space of grain-oriented electrical steel becomes smaller,which reflects as a variation of MBN in the macroscopic magnetic properties.Through correlation formula derivation of MBNrms and equilibrium distance between domain walls,effect of domain refinement on grain-oriented electrical steel was also interpreted,and optimum equilibrium distance between domain walls was determined. | Hao Wang Changsheng Li Tao Zhu Ban Cai Gang Huo Nasir Mohamed | 2013 | Journal of Materials Science & Technology2013，29，7： | 3 |
| 20 | Efect of Ball Scribing on Relative Permeability of Grain-oriented Electrical Steel显示文摘Efect of ball scribing on relative permeability of conventional grain-oriented(CGO)and high permeability grain-oriented(HGO)electrical steel was investigated.The samples were scribed with spacing of 2 mm,4 mm,8 mm and 16 mm.The results show that after ball scribing with 16 mm width at 1.0 T,relative permeability of both℃GO and HGO steels was increased by 109%and 80%,respectively.Relative permeability rises as the scribing space increases,with the movement of the peak value of relative permeability to a higher flux density.Relational models describing relative permeability and flux density were constructed with high accuracy based on experimental data.The experimental data curves were analyzed during the magnetizing process. | Hao WANG Changsheng LI Tao ZHU Nkwachukwu Chukwuchekwa Ban CAI Gang HUO | 2013 | Acta Metallurgica Sinica(English Letters)2013，26，5： | 2 |
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