| 1 | 闽江下游全新世孢粉记录的植被与环境变化显示文摘为了揭示闽江下游闽侯盆地第四纪沉积环境演变与人类活动历史,对闽江下游闽侯盆地庄边山遗址附近的FZ5钻孔进行了孢粉、硅藻分析。结果表明:闽侯盆地全新世海侵初始发生时间约为9 000 cal.a B.P.,至7 500cal.a B.P.达到最大海侵范围,晚全新世海退的发生时间在闽侯盆地大约为1 900 cal.a B.P.。孢粉分析证实了当地早中全新世温暖的亚热带气候以及浓密的常绿阔叶林植被。在7 500―1 900 cal.a B.P.之间无论植被覆盖还是气候环境均比较稳定,高海平面时期的适宜气候以及海湾环境也有利于当地以渔猎经济方式为主的新石器文化(昙石山文化)的兴起和发展。自1 900 cal.a B.P.以后,孢粉组合突变为以先锋植物芒萁属(Dicranopteris)、禾本科(Poaceae)、松属(Pinus)等类型为主,揭示了当地植被受到人类农业刀耕火种的影响而发生显著变化。高含量的禾本科花粉(含一定数量的水稻)仅在剖面上部明显增加这一事实,证实了闽侯和福州一带水稻种植的大面积发展始于距今1 900 a前后。 | 乐远福 郑卓 Barry V ROLETT 黄博津 黄康有 Rachid CHEDDADI | 2016 | 热带地理2016,36,3: | 7 |
| 2 | Macrophage secretory products induce an inflammatory phenotype in hepatocytes显示文摘AIM:To investigate the influence of macrophages on hepatocyte phenotype and function.METHODS:Macrophages were differentiated from THP-1 monocytes via phorbol myristate acetate stimulation and the effects of monocyte or macrophageconditioned medium on HepG2 mRNA and protein expression determined.The in vivo relevance of these findings was confirmed using liver biopsies from 147 patients with hepatitis C virus(HCV)infection.RESULTS:Conditioned media from macrophages,but not monocytes,induced a transient morphological change in hepatocytes associated with upregulation of vimentin(7.8±2.5-fold,P=0.045)and transforming growth factor(TGF)-β1(2.6±0.2-fold,P<0.001)and downregulation of epithelial cadherin(1.7±0.02-fold,P=0.017)mRNA expression.Microarray analysis revealed significant upregulation of lipocalin-2(17-fold,P <0.001)and pathways associated with inflammation,and substantial downregulation of pathways related to hepatocyte function.In patients with chronic HCV,realtime polymerase chain reaction and immunohistochemistry confirmed an increase in lipocalin-2 mRNA(F0 1.0 ±0.3,F1 2.2±0.2,F2 3.0±9.3,F3/4 4.0±0.8,P= 0.003)and protein expression(F1 1.0±0.5,F2 1.3± 0.4,F3/4 3.6±0.4,P=0.014)with increasing liver injury.High performance liquid chromatography-tandem mass spectrometry analysis identified elevated levels of matrix metalloproteinase(MMP)-9 in macrophageconditioned medium,and a chemical inhibitor of MMP-9 attenuated the change in morphology and mRNA expression of TGF-β1(2.9±0.2 vs 1.04±0.1,P<0.001) in macrophage-conditioned media treated HepG2 cells.In patients with chronic HCV infection,hepatic mRNA expression of CD163(F0 1.0±0.2,F1/2 2.8±0.3,F3/4 5.3±1.0,P=0.001)and MMP-9(F0 1.0±0.4,F1/2 2.8±0.3,F3/4 4.1±0.8,P=0.011)was significantly associated with increasing stage of fibrosis.CONCLUSION:Secreted macrophage products alter the phenotype and function of hepatocytes,with increased expression of inflammatory mediators,suggesting that hepatocytes actively participate in liver injury. | Michelle Melino Victoria L Gadd Gene V Walker Richard Skoien Helen D Barrie Dinesh Jothimani Leigh Horsfall Alun Jones Matthew J Sweet Gethin P Thomas Andrew D Clouston Julie R Jonsson Elizabeth E Powell | 2012 | World Journal of Gastroenterology2012,18,15: | 3 |