| 1 | Ghrelin protects myocardium from isoproterenol-induced injury in rats显示文摘AIM: To investigate the cardiac protective effects of ghrelin in rat with myocardial injury induced by isoproterenol (ISO). METHODS: Rats were subcutaneously injected ISO 40mg.kg^-1.d^-1 with or without ghrelin 1 or 10nmol.kg^-1.d^-1 for 2d. Hemodynamic parameters including mean arterial blood pressure and left ventricular pressure were measured at 12h after the last injection with ISO and/or ghrelin. Plasma lactate dehydrogenase (LDH) activity, plasma and myocardial contents of malondialdehyde (MDA), and conjugated diene were measured. Plasma ghrelin and endothelin-1 levels were assayed using radioimmunoassay methods. Endothelin-1 and ghrelin mRNA were determined using RT-PCR. RESULTS: About 45%(5/11) of rats after treatment with ISO alone died during experimental periods. However, no rats died after administration with ghrelin 10nmol.kg^-1.d^-1(0/11, P<0.05). Ghrelin also obviously ameliorated the hemodynamic disturbance in rats induced by ISO. The plasma LDH activity,contents of myocardial and plasma MDA, and conjugated diene level in plasma in ISO+G10nmol.kg^-1.d^-1 group were decreased by 28%, 34%, 73%, and 38% compared with those of ISO group (all P<0.01) respectively. ISO-induced endothelin-1 mRNA over-expression was inhibited and endothelin-1 level in plasma were inhibited by ghrelin 1 and 10nmol.kg^-1.d^-1. The ghrelin levels in plasma and ghrelin mRNA in myocardium were increased in the rats after injection of ISO. The plasma ghrelin level was further increased after ghrelin administration. CONCLUSION: Ghrelin has a protective effect against ISO-induced myocardial injury. | LinCHANG JingZHAO Gui-zhongLI BinGENG Chun-shuiPAN Yong-fenQI Chao-shuTANG | 2004 | Acta Pharmacologica Sinica2004，25，9： | 21 |
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