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| 1 | Low-frequency pulsed electromagnetic field pretreated bone marrow-derived mesenchymal stem cells promote the regeneration of crush-injured rat mental nerve显示文摘Bone marrow-derived mesenchymal stem cells(BMSCs) have been shown to promote the regeneration of injured peripheral nerves. Pulsed electromagnetic field(PEMF) reportedly promotes the proliferation and neuronal differentiation of BMSCs. Low-frequency PEMF can induce the neuronal differentiation of BMSCs in the absence of nerve growth factors. This study was designed to investigate the effects of low-frequency PEMF pretreatment on the proliferation and function of BMSCs and the effects of low-frequency PEMF pre-treated BMSCs on the regeneration of injured peripheral nerve using in vitro and in vivo experiments. In in vitro experiments, quantitative DNA analysis was performed to determine the proliferation of BMSCs, and reverse transcription-polymerase chain reaction was performed to detect S100(Schwann cell marker), glial fibrillary acidic protein(astrocyte marker), and brain-derived neurotrophic factor and nerve growth factor(neurotrophic factors) m RNA expression. In the in vivo experiments, rat models of crush-injured mental nerve established using clamp method were randomly injected with low-frequency PEMF pretreated BMSCs, unpretreated BMSCs or PBS at the injury site(1 × 106 cells). Di I-labeled BMSCs injected at the injury site were counted under the fluorescence microscope to determine cell survival. One or two weeks after cell injection, functional recovery of the injured nerve was assessed using the sensory test with von Frey filaments. Two weeks after cell injection, axonal regeneration was evaluated using histomorphometric analysis and retrograde labeling of trigeminal ganglion neurons. In vitro experiment results revealed that low-frequency PEMF pretreated BMSCs proliferated faster and had greater m RNA expression of growth factors than unpretreated BMSCs. In vivo experiment results revealed that compared with injection of unpretreated BMSCs, injection of low-frequency PEMF pretreated BMSCs led to higher myelinated axon count and axon density and more Di I-labeled neurons in the trigeminal ganglia, contributing to rapider functional recovery of injured mental nerve. These findings suggest that low-frequency PEMF pretreatment is a promising approach to enhance the efficacy of cell therapy for peripheral nerve injury repair. | NaRi Seo Sung-Ho Lee Kyung Won Ju JaeMan Woo BongJu Kim SoungMin Kim Jeong Won Jahng Jong-Ho Lee | 2018 | Neural Regeneration Research2018，13，1： | 11 |
| 2 | Recombinant human fibroblast growth factor-2 promotes nerve regeneration and functional recovery after mental nerve crush injury显示文摘Several studies have shown that fibroblast growth factor-2(FGF2) can directly affect axon regeneration after peripheral nerve damage. In this study, we performed sensory tests and histological analyses to study the effect of recombinant human FGF-2(rh FGF2) treatment on damaged mental nerves. The mental nerves of 6-week-old male Sprague-Dawley rats were crush-injured for 1 minute and then treated with 10 or 50 μg/m L rh FGF2 or PBS in crush injury area with a mini Osmotic pump. Sensory test using von Frey filaments at 1 week revealed the presence of sensory degeneration based on decreased gap score and increased difference score. However, at 2 weeks, the gap score and difference score were significantly rebounded in the mental nerve crush group treated with 10 μg/m L rh FGF2. Interestingly, treatment with 10 μg/m L rh FGF had a more obviously positive effect on the gap score than treatment with 50 μg/m L rh FGF2. In addition, retrograde neuronal tracing with Dil revealed a significant increase in nerve regeneration in the trigeminal ganglion at 2 and 4 weeks in the rh FGF2 groups(10 μg/m L and 50 μg/m L) than in the PBS group. The 10 μg/m L rh FGF2 group also showed an obviously robust regeneration in axon density in the mental nerve at 4 weeks. Our results demonstrate that 10 μg/m L rh FGF induces mental nerve regeneration and sensory recovery after mental nerve crush injury. | Sung Ho Lee Wei-Peng Jin Na Ri Seo Kang-Mi Pang Bongju Kim Soung-Min Kim Jong-Ho Lee | 2017 | Neural Regeneration Research2017，12，4： | 2 |
| 3 | A production distribution coordinating model for third party logistics partnership显示文摘 | Hosang J Chen F Bongju J | 2008 | Computers & Industrial Engineering2008，59，1： | 1 |
| 4 | Product sequencing problem in Mixed-Model Assembly Line to minimize unfinished works显示文摘 | Siwon Kim Bongju Jeong | 2007 | Computers & Industrial Engineering2007，，53： | 1 |
| 5 | Decentralized supply chain planning framework for third party logistics partnership 显示文摘 | Hosang Jung F Frank Chen Bongju Jeong | 2008 | Computers & Industrial Engineering2008，55，2： | 1 |
| 6 | At atom-spheric-pressure nitrogen-plasma jet produced frommicrodischarges in a porous dielectric 显示文摘 | Yongcheol Hong Seungryul Yoo Bongju Lee | 2011 | Journal ofElectrostatics2011，69，： | 1 |
| 7 | Beta‐lapachone inhibits pathological retinal neovascularization in oxygen‐induced retinopathy via regulation of HIF ‐1α显示文摘 | Sung Wook Park Jin Hyoung Kim Ko‐Eun Kim Moon Hee Jeong Hyunsung Park Bongju Park Young‐Ger Suh Woo Jin Park Jeong Hun Kim | 2014 | J Cell Mol Med2014，，5： | 1 |
| 8 | An available-to-promise system for TFT LCD manufacturing in supply chain显示文摘 | Bongju Jeong Seung-Bae Sim Ho-Sang Jecong | 2002 | Computer & Industrial Engineering2002，43，： | 1 |
| 9 | An available-to-promise system for TFT LCD manufacturing in supply chain显示文摘 | Jeong Bongju Sire SeungBae Jeong HoSang | 2002 | Computers and Industrial Engineering2002，43，12： | 1 |
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