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| 1 | No evidence of HPV DNA in esophageal squamous cell carcinoma in a population of Southern Brazil显示文摘AIM:To investigate the association between human papillomavirus(HPV)and esophageal squamous cell carcinoma(ESCC)in southern Brazil.METHODS:We studied 189 esophageal samples from125 patients from three different groups:(1)102 biopsies from 51 patients with ESCC,with one sample from the tumor and another from normal esophageal mucosa distant from the tumor;(2)50 esophageal biopsies from 37 patients with a previous diagnosis of head and neck squamous cell carcinoma(HNSCC);and(3)37 biopsies from esophageal mucosa with normal appearance from 37 dyspeptic patients,not exposed to smoking or alcohol consumption.Nested-polymerase chain reaction(PCR)with the MY09/11 and GP5/6 L1primers was used to detect HPV L1 in samples fixed in formalin and stored in paraffin blocks.All PCR reactions were performed with a positive control(cervicovaginal samples),with a negative control(Human Genomic DNA)and with a blank reaction containing all reagents except DNA.We took extreme care to prevent DNA contamination in sample collection,processing,and testing.RESULTS:The histological biopsies confirmed the diagnosis of ESCC in 52 samples(51 from ESCC group and 1 from the HNSCC group)and classified as well differentiated(12/52,23.1%),moderately differentiated(27/52,51.9%)or poorly differentiated(7/52,13.5%).One hundred twenty-eight esophageal biopsies were considered normal(51 from the ESCC group,42 from the HNSCC group and 35 from dyspeptic patients).Nine had esophagitis(7 from the HNSCC and 2 from dyspeptic patients).Of a total of 189 samples,only 6 samples had insufficient material for PCR analysis:1 from mucosa distant from the tumor in a patient with ESCC,3from patients with HNSCC and 2 from patients without cancer.In 183 samples(96.8%)GAPDH,G3PDH and/orβ-globin were amplified,thus indicating the adequacy of the DNA in those samples.HPV DNA was negative in all the 183 samples tested:52 with ESCC,9 with esophagitis and 122 with normal esophageal mucosa.CONCLUSION:There was no evidence of HPV infection in different ESCC from southern Brazil. | Luís Carlos Moreira Antunes Joo Carlos Prolla Antonio de Barros Lopes Marta Pires da Rocha Renato Borges Fagundes | 2013 | World Journal of Gastroenterology2013，19，39： | 4 |
| 2 | Perspectives on new biomarkers in gastric cancer: Diagnostic and prognostic applications显示文摘Gastric cancer is considered one of the most deadly tumors worldwide. Even with the decline in its incidence, the mortality rate of this disease has remained high, mainly due to its late diagnosis and to the lack of precise prognostic markers. The main purpose of this review is to present genetic, epigenetic and proteomic molecular markers that may be used in a diagnostic and prognostic manner and to discuss the pros and cons of each type of marker for improving clinical practice. In this sense, we observed that the use of genetic markers, especially mutations and polymorphisms, should be carefully considered, as they are strongly affected by ethnicity. Proteomic-based markers show promise, but the higher costs of the associated techniques con-tinue to make this approach expensive for routine use. Alternatively, epigenetic markers appear to be very promising, as they can be detected in bodily fluids as well as tissues. However, such markers must be used carefully because epigenetic changes may occur due to environmental factors and aging. Despite the advances in technology and its access, to date, there are few defined biomarkers of prognostic and diagnostic use for gastric tumors. Therefore, the use of a panel of several approaches(genetic, epigenetic and proteomic) should be considered the best alternative for clinical practice. | Danilo do Rosário Pinheiro Wallax Augusto Silva Ferreira Mariceli Baia Leo Barros Mariana Diniz Araújo Symara Rodrigues-Antunes Bárbara do Nascimento Borges | 2014 | World Journal of Gastroenterology2014，20，33： | 4 |
| 3 | Systematic review of noninvasive treatments to arrest dentin non-cavitated caries lesions显示文摘AIM: To systematically review the literature on the efficacy of noninvasive methods of arresting the progression of non-cavitated occlusal carious lesions in dentin.METHODS: The Medline/Pub Med,LILACS,Sci ELO and Scopus databases were searched to identify relevant publications through to November 2013.Only clinical trials evaluating the ability of noninvasive methods to arrest the progression of occlusal non-cavitated carious lesions in dentin were included.Screening,data extraction and quality assessment were conducted independently and in duplicate.RESULTS: Of 167 citations identified,nine full text articles were screened and five were included in the analysis.All papers reported on occlusal fissure sealing using a self-curing glass ionomer(n = 1) or resin-based(n = 4) sealant.Only the use of resin-based sealant to obliterate occlusal fissures arrested the progression of non-cavitated occlusal carious lesions in dentin.CONCLUSION: Occlusal fissure sealing with a resin-based sealant may arrest the progression of non-cavitated occlusal dentinal caries.Further clinical trials with longer follow-up times should be performed to increase scientific evidence. | Isauremi Vieira de Assun??o Giovanna de Fátima Alves da Costa Boniek Castillo Dutra Borges | 2014 | World Journal of Clinical Cases2014，2，5： | 3 |
| 4 | Immune response by nasal delivery of hepatitis B surface antigen and codelivery of a CpG ODN in alginate coated chitosan nanoparticles显示文摘 | Borges O Cordeiro-da-Silva A Tavares J | 2008 | EurJ Pharm Biopharm2008，69，2： | 1 |
| 5 | Kinetic Resolution of(R,S)-1,2-Isopropylidene Glycerol(Solketal)Ester Derivatives by Lipases显示文摘 | Machado A C O Da Silva A A T Borges C P | 2011 | J Mol Catal B:Enzym2011，69，1： | 1 |
| 6 | Simultaneous follow-up of mouse colon lesions by colonoscopy and endoluminal ultrasound biomicroscopy显示文摘AIM:To evaluate the potential use of colonoscopy and endoluminal ultrasonic biomicroscopy(eUBM)to track the progression of mouse colonic lesions.METHODS:Ten mice were treated with a single azoxy-methane intraperitoneal injection(week 1)followed by seven days of a dextran sulfate sodium treatment in their drinking water(week 2)to induce inflammationassociated colon tumors.eUBM was performed simultaneously with colonoscopy at weeks 13,17-20 and21.A 3.6-F diameter 40 MHz mini-probe catheter was used for eUBM imaging.The ultrasound mini-probe catheter was inserted into the accessory channel of a pediatric flexible bronchofiberscope,allowing simultaneous acquisition of colonoscopic and eUBM images.During image acquisition,the mice were anesthetized with isoflurane and kept in a supine position over a stainless steel heated surgical waterbed at 37℃.Both eUBM and colonoscopic images were captured and stored when a lesion was detected by colonoscopy or when the eUBM image revealed a modified colon wall anatomy.During the procedure,the colon was irrigated with water that was injected through a flush port on the mini-probe catheter and that acted as the ultrasound coupling medium between the transducer and the colon wall.Once the acquisition of the last eUBM/colonoscopy section for each animal was completed,the colons were fixed,paraffin-embedded,and stained with hematoxylin and eosin.Colon images acquired at the first time-point for each mouse were compared with subsequent eUBM/colonoscopic images of the same sites obtained in the following acquisitions to evaluate lesion progression.RESULTS:All 10 mice had eUBM and colonoscopic images acquired at week 13(the first time-point).Two animals died immediately after the first imaging acquisition and,consequently,only 8 mice were subjected to the second eUBM/colonoscopy imaging acquisition(at the second time-point).Due to the advanced stage of colonic tumorigenesis,5 animals died after the second time-point image acquisition,and thus,only three were subjected to the third eUBM/colonoscopy imaging acquisition(the third time-point).eUBM was able to detect the four layers in healthy segments of colon:the mucosa(the first hyperechoic layer moving away from the mini-probe axis),followed by the muscularis mucosae(hypoechoic),the submucosa(the second hyperechoic layer)and the muscularis externa(the second hypoechoic layer).Hypoechoic regions between the mucosa and the muscularis externa layers represented lymphoid infiltrates,as confirmed by the corresponding histological images.Pedunculated tumors were represented by hyperechoic masses in the mucosa layer.Among the lesions that decreased in size between the first and third time-points,one of the lesions changed from a mucosal hyperplasia with ulceration at the top to a mucosal hyperplasia with lymphoid infiltrate and,finally,to small signs of mucosal hyperplasia and lymphoid infiltrate.In this case,while lesion regression and modification were observable in the eUBM images,colonoscopy was only able to detect the lesion at the first and second time-points,without the capacity to demonstrate the presence of lymphoid infiltrate.Regarding the lesions that increased in size,one of them started as a small elevation in the mucosa layer and progressed to a pedunculated tumor.In this case,while eUBM imaging revealed the lesion at the first time-point,colonoscopy was only able to detect it at the second time-point.All colonic lesions(tumors,lymphoid infiltrate and mucosal thickening)were identified by eUBM,while colonoscopy identified just76%of them.Colonoscopy identified all of the colonic tumors but failed to diagnose lymphoid infiltrates and increased mucosal thickness and failed to differentiate lymphoid infiltrates from small adenomas.During the observation period,most of the lesions(approximately67%)increased in size,approximately 14%remained unchanged,and 19%regressed.CONCLUSION:Combining eUBM with colonoscopy improves the diagnosis and the follow-up of mouse colonic lesions,adding transmural assessment of the bowel wall. | Rossana C Soletti Kelly Z Alves Marcelo AP de Britto Dyanna G de Matos Mnica Soldan Helena L Borges Joo C Machado | 2013 | World Journal of Gastroenterology2013，19，44： | 1 |
| 7 | Enteric bacterial catalysts for fuel ethanol production 显示文摘 | Ingram L O Aldrich H C Borges A C C | 1999 | Biotechnology Progress1999，15，5： | 1 |
| 8 | Endovascular Treatment of Acute Portal Vein Thrombosis After Liver Transplantation in a Child显示文摘 | Francisco Cesar Carnevale Marcus Vinicius Borges Airton Mota Moreira Giovanni Guido Cerri Jo?o Gilberto Maksoud | 2006 | CardioVascular and Interventional Radiology2006，，3： | 1 |
| 9 | Thrombopoietin but not erythropoietin promotes viability and inhibits apoptosis of multipotent murinehematopoietic progenitor cells in vitro 显示文摘 | Borge O J Ramsfjell V Veiby O P | 1996 | Blood1996，88，8： | 1 |
| 10 | A real-time visualization tool for forest eccsystem management decision support显示文摘 | Falcao A O Santos M P D Borges J G | 2006 | Computers and Electronics in Agriculture2006，53，： | 1 |
| 11 | Effect of sediment load on soil detachment and deposition in rills显示文摘 | Merten G H Nearing M A Borges A L O | 2001 | SSSA J2001，65，： | 1 |
| 12 | Chicken Feath- er Keratin Films Plasticized with Polyethylene Glycol显示文摘 | Silvia Maria Martelli Jo? o Borges Laurindo | 2012 | In ternational Journal of Polymeric Materials2012，，61： | 1 |
| 13 | A Network-based Space-vector PWM Controller for voltagefed Inverter Induction Motor Drive显示文摘 | PINTO J O P BOSE B K BORGES L E | 2000 | IEEE Trans on Ind(S7803-5589)Applicat2000，36，6： | 1 |
| 14 | Pathogenicity of Drechslera sorghicola isolates on sorghum in Venezuela显示文摘 | Borges O L | 1983 | Plant Disease1983，67，9： | 1 |
| 15 | Partial oxidation of ethanol over Pd/CeO2 and Pd/Y2 O3 catalysts显示文摘 | COSTA L O O SILVA A M BORGES L E P MATTOS L V NORONHA F B | | 0，，3： | 1 |
| 16 | Gastroparesis is associated with oxytocin deficiency, oesophageal dysmo- tility with hyperCCKemia, and autonomic neuropathy with hypergastrinemia显示文摘 | Borg J Melander O Johansson L | 2009 | BMC Gastroenterol2009，9，： | 1 |
| 17 | High levels of antigen Specific islet antibodies predict future cell failure in patients with onset of diabetes in adult age显示文摘 | Borg H Gottsater A Landim O | 2001 | J Clin Endocrinol Metab2001，86，7： | 1 |
| 18 | CAG repeat length in the androgen receptor gene is related to age at diagnosis of prostate cancer and response to endocrine therapy,but not to prostate cancer risk显示文摘 | Bratt O Borg A Kristoffersson U | 1998 | Brit J Cancer1998，81，4： | 1 |
| 19 | A real time visualization tool for forest ecosystem management decision support显示文摘 | Falcao A O Santos M P Borges J G | 2006 | Computers and Electronics in Agriculture2006，53，： | 1 |
| 20 | Features of In Vitro Ultrasound Biomicroscopic Imaging and Colonoscopy for Detection of Colon Tumor in Mice显示文摘 | Kelly Z. Alves Helena L. Borges Rossana C. Soletti Anália L.P. Viana Lorena I. Petrella M?nica Soldan Vera L. Chagas Alberto Schanaider Jo?o C. Machado | 2011 | Ultrasound in Medicine & Biology2011，，12： | 1 |
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