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| 1 | Absolute copy number differences of Y chromosomal genes between crossbred (Bos taurus×Bos indicus) and Indicine bulls显示文摘Background: The Y chromosome in mammal is paternally inherited and harbors genes related to male fertility and spermatogenesis. The unique intra-chromosomal recombination pattern of Y chromosome and morphological difference of this chromosome between Bos taurus and Bos indicus make it an ideal model for studying structural variation, especially in crossbred(Bos taurus × Bos indicus) bulls. Copy Number Variation(CNV) is a type of genomic structural variation that gives information complementary to SNP data. The purpose of this study was to find out copy number differences of four Y chromosomal spermatogenesis-related candidate genes in genomic DNA of crossbred and purebred Indicine bulls. Result: Four Y chromosomal candidate genes of spermatogenesis namely, sex determining gene on Y chromosome(SRY), DEAD box polypeptide 3-Y chromosome(DDX3Y), Ubiquitin specific peptidase 9, Y-linked(USP9Y), testis-specific protein on Y chromosome(TSPY) were evaluated. Absolute copy numbers of Y chromosomal genes were determined by standard curve-based quantitative real time PCR. Copy numbers of SRY and TSPY genes per unit amount of genomic DNA are higher in crossbred than Indicine bulls. However, no difference was observed in DDX3Y and USP9Y gene copy numbers between two groups. Conclusion: The present study demonstrates that the structural organization of Y chromosomes differs between crossbred and Indicine bulls which are reproductively healthy as observed from analysis of semen attributes. The absolute copy numbers of SRY and TSPY genes in unit mass of genomic DNA of crossbred bulls are significantly higher than Indicine bulls. No alteration in absolute copies of DDX3Y and USP9Y gene was found between the genome of crossbred and Indicine bulls. This study suggests that the DDX3Y and USP9Y are likely to be single copy genes in the genome of crossbred and Indicine bulls and variation in Y chromosome length between crossbred and Indicine bulls may be due to the copy number variation of SRY gene and TSPY array. | Ayan Mukherjee Gulshan Dass Jagan Mohanarao G Moloya Gohain Biswajit Brahma Tirtha Kumar Datta Sachinandan De | 2013 | Journal of Animal Science and Biotechnology2013，4，4： | 3 |
| 2 | Identification and characterization of Rhipicephalus ( Boophilus ) microplus and Haemaphysalis bispinosa ticks (Acari: Ixodidae) of North East India by ITS2 and 16S rDNA sequences and morphological analysis显示文摘 | Rajeev Kungur Brahma Vishal Dixit Arun K. Sangwan Robin Doley | 2014 | Experimental and Applied Acarology2014，，2： | 2 |
| 3 | Future directions in myoma research显示文摘 | Brahma P K Martel K M Christman G M | 2006 | Obstet Gynecol Clin North Am2006，33，1： | 1 |
| 4 | Surgical treatment of trigeminal neuralgia ;comparison of microvascular decompression, percutaneous ablation, and stereotactic radiosurgery 显示文摘 | Cowan A Jr Brahma B | 2003 | Neurosurgery2003，8，3： | 1 |
| 5 | Development of adaptive protection sche- me for distribution systems with high penetration of distributed ge- neration显示文摘 | Brahma S M Girgis A A | 2004 | IEEE Trans on Power Delivery2004，19，1： | 1 |
| 6 | Development of Adaptive Scheme for Distribution System with High Penetration of Distributed Generation显示文摘 | Brahma S M Girgis A A | 2004 | IEEE Trans on Power Delivery2004，19，1： | 1 |
| 7 | Characterization of a dimeric unfolding intermediate of bovine serum albumin under mildly acidic condition 显示文摘 | Amrita Brahma Chhabinath Mandal Debasish Bhattacharyya | 2005 | Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics2005，1751，2： | 1 |
| 8 | Development of Adaptive Protection Scheme for Distribution System with High Penetration of Distributed Generation 显示文摘 | BRAHMA S M GIRGIS A A | | IEEE Transactions on Power Delivery0，19，1： | 1 |
| 9 | Comparison of corneal thickness measurements using ultrasound and Orbscan slit-scanning topography in normal and post-LASIK eyes显示文摘 | Chakrabarti HS Craig JP Brahma A | 2001 | J Cataract Refract Surg2001，27，11： | 1 |
| 10 | Surgical treatment of trigeminal neuralgia:Comparlson of microvascular decompression, Percutaneous ablation and stereotactic radiosurgery surgical management of chronic pain : Article 显示文摘 | Cowan Jr Brahma BA Sagher OR | 2003 | Neurosurgery2003，8，： | 1 |
| 11 | Densifieation, sintering reactions, and properties of titanium diboride with titanium disilide as a sintering aid 显示文摘 | Brahma R G Bikramjit B | 2007 | Journal of America Ceramic Society2007，11，90： | 1 |
| 12 | An epidemic of mass hysteria in a village in West Bengal 显示文摘 | Chowdhury AN Brahma A | 2005 | Indian J Psychiatry2005，47，2： | 1 |
| 13 | Fecal and urinary incontinence after vaginal delivery with anal sphincter disruption in an obstetric unit in the United States显示文摘 | Fenner DE Genberg B Brahma P | 2003 | Am J Obstet Gynecol2003，189，： | 1 |
| 14 | General supervisory control policy for the energy optimization of charge-sustaining hybrid electric vehicles 显示文摘 | Paganelli G Ercole G Brahma A | 2001 | JSAE Review2001，，22： | 1 |
| 15 | Simplex and duplex PCR assays for species specific identification of cattle and buffalo milk and cheese显示文摘 | Sachinandan De Biswajit Brahma Shamik Polley Ayan Mukherjee Deepak Banerjee Moloya Gohaina Karan Pratap Singh Rameswar Singh Tirtha Kumar Datta Surender Lal Goswami | 2010 | Food Control2010，，5： | 1 |
| 16 | Development of adaptive protection schemes for distribution systems with high penetration of distribution generation显示文摘 | Brahma S M Girgis A A | 2004 | IEEE Trans on Power Delivery2004，19，1： | 1 |
| 17 | Traffic management in wireless sensor networks: decoupling congestion control and thir- hess显示文摘 | BRAHMA S CHATTERJEE M KWIAT K | 2012 | Computer Communications2012，35，6： | 1 |
| 18 | Systematic characterization of integrated circuit standard components as stimulated by scanning laser beam 显示文摘 | GLOWACKI A M BRAHMA S K SUZUKI H | 2007 | IEEE Transactions on Device and Materials Reliability2007，7，1： | 1 |
| 19 | Adverse drug reactions in the elderly显示文摘 | Brahma DK Wahlang JB Marak MD | 2013 | J Pharmacol Pharmacother2013，4，2： | 1 |
| 20 | General Supervisory Control Policy for the Energy Optimization of Charge-sustaining Hy- brid Electric Vehicles 显示文摘 | Paganelli G Ercole G Brahma A | 2001 | JSAE Review2001，22，： | 1 |
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