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| 1 | Genetic improvement of Trichoderma ability to induce systemic resistance显示文摘The beneficial applications of Trichoderma spp. in agriculture include not only the control of plant pathogens, but also the improvement of plant growth, micronutrient availability, and plant tolerance to abiotic stress. In addition, it has been suggested that these fungi are able to increase plant disease resistance by activating induced systemic resistance (ISR) . The mode of action of these beneficial fungi in the Trichoderma -plant-pathogen interaction are many, complex and not completely understood. Numerous lytic enzymes have been characterized, the encoding genes (ech42 gluc78, nag1 from T. atroviride strain P1) cloned, and their role in biocontrol demonstrated. The corresponding biocontrol-related inducible promoters have been used in a reporter system based on the Aspergillus niger glucose oxidase gene (goxA) to monitor biocontrol activity. Glucose oxidase catalyzes the oxygen-dependent oxidation of D-glucose to D-glucono-1,5-lactone and hydrogen peroxide; this latter compound is known to have an antifungal effect and activate the plant defence cascade, thus increasing resistance to pathogen attack. T. atroviride P1 transformants with various promoters gox were tested as seed coating treatments on bean seeds planted in soil infested with a soilborne fungal pathogen. Successively, the emergent leaves were inoculated with a foliar pathogen to determine the effect of the GOX transformants on biocontrol and resistance to pathogen attack. Inoculations with the P1-GOX transformants not only reduced disease symptoms caused by a soil pathogen, but also the lesions of various foliar pathogens applied far from the Trichoderma colonization, thus activating ISR. A similar approach is being use to genetically improve T. harzianum T22, a rhizosphere competent and commercially marketed strain not transformed yet, by using four different gox gene constructs under the control of constitutive and inducible promoters. Plasmids have been introduced in Trichoderma by protoplasts co-transformation. hygromicin resistant progeny selected, and mitotically stable transformants analysed to confirm the presence of the novel enzyme activity. Progenies are being tested for biocontrol ISR inducing activity. | Ciliento R Woo S L Di Benedetto P Ruocco M Scala F Soriente I Ferraioli S Brunner K Zeilinger S Mach R L Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 2 |
| 2 | Testosterone inhibits expression of inducible nitric oxide synthase in murine macrophages显示文摘 | Brunner M Moeslinger T | 2000 | Life Sci2000，68，4： | 1 |
| 3 | Colonic spread and serum pharmacokinetics of budesonide foam in patients with mildly to moderately active ulcerative colitis显示文摘 | Brunner M Vogelsang H Greinwald R | 2005 | Aliment Pharmacol Ther2005，22，5： | 1 |
| 4 | Service Management in Multi-party Active Networks 显示文摘 | Brunner M Stadler R | 2000 | IEEE Communications Magazine2000，38，3： | 1 |
| 5 | Service management multiparty active networks显示文摘 | Brunner M TIK Stadler R | 2000 | IEEE Communications Magazine2000，38，3： | 1 |
| 6 | Service Management in Multi-Party Active Networks 显示文摘 | Brunner M Stadler R | 2000 | IEEE Communications Magazine2000，38，3： | 1 |
| 7 | Diagnosis of viral myocarditis by cardiac magnetic resonance and viral genome detection in peripheral blood显示文摘 | Jeserich M Brunner E Kandolf R | 2013 | The international journal of cardiovascular imaging2013，29，1： | 1 |
| 8 | Validation of urea as anendogenous reference compound for the in vivo calibration of Micro-dialysis probes显示文摘 | Brunner M Joukhadar C Schmid R | 2000 | Life Sci2000，67，： | 1 |
| 9 | Porphyrin platinum conjugates-new aims显示文摘 | Arndt M R Treittinger B | 2004 | Inorganica Chimica Acta2004，357，： | 1 |
| 10 | Arterial stiffness, physi- cal function,and functional limitation: the Whitehall Ⅱ Study显示文摘 | Brunner E J Shipley M J Witte D R | 2011 | Hypertension2011，57，5： | 1 |
| 11 | Fiber optic rate gyros as replacements for mechanical gyros显示文摘 | Dyott R Allen D Brunner J Kidwell R Miller R | 1998 | American Institute of Aeronautics and Astronautics1998，4401，： | 1 |
| 12 | FT-NIR spectroscopy and wood identification显示文摘 | Brunner M Mugster R Trenka E | 1996 | Holzforschung1996，50，2： | 1 |
| 13 | Com- parative mapping of the gene coding for inhibin-ot(INHA) to chromosome 2 in sheep and cattle 显示文摘 | Brunner R M Goldammer T Hiendleder S | 1995 | Mammalian Ge- nome1995，6，4： | 1 |
| 14 | Colonic spread and serumpharmacokinetics of budesonide foam in patients with mildly to moderately active ulcerative colitis显示文摘 | Brunner M Vogelsang H Greinwald R | 2012 | Aliment Pharmacol Ther2012，22，2： | 1 |
| 15 | Comparative mapping of the gene coding for inhibin-α (INHA) to chromosome 2 in sheep and cattle显示文摘 | Brunner R M Goldammer T Hiendleder S | 1995 | Mamm Genome1995，6，4： | 1 |
| 16 | Fas and Fas ligand in gut and liver显示文摘 | Pinkoski M J Brunner T Green D R | 2000 | Am J Physiol Gastrointest Liver Physiol2000，278，3： | 1 |
| 17 | NAMD2:greater scalability for parallel molecular dynamics显示文摘 | Kale L Skeel R Bhandarkar M Brunner R | | 0，，： | 1 |
| 18 | How to Improve Adherence with Prescribed Treatment in Hypertensive Patients? 显示文摘 | Waber B Burnier M Brunner H R | 2000 | J Cardiovasc Pharmacol2000，35，3： | 1 |
| 19 | Spatial filtering and selection of optimized components in four class motor imagery EEG data using independent components analysis 显示文摘 | Brunner C Naeem M Leeb R | 2007 | Pattern Recognition Letters2007，28，8： | 1 |
| 20 | Transgenic sterility in Populus:expression properties of the poplar PTLF,Agrobacterium NOS and two minimal 35S promoters in vegetative tissues显示文摘 | WEI H MEILAN R BRUNNER A M | 2006 | Tree Physiology2006，26，： | 1 |
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