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| 1 | Regulation of blood-testis barrier dynamics by the mT0RCl/rpS6 signaling complex:an in vitro study显示文摘During spermatogenesis, developi ng germ cells that lack the cellular ultrastructures of filopodia and lamellipodia gen erally found in migrating cells, such as macrophages and fibroblasts, rely on Sertoli cells to support their transport across the seminiferous epithelium. These in elude the transport of preleptote ne spermatocytes across the blood-testis barrier (BTB), but also the transport of germ cells, in particular developing haploid spermatids, across the seminiferous epithelium, that is to and away from the tubule lumen, depending on the stages of the epithelial cycle. On the other hand, cell junctions at the Sertoli cell-cell and Sertoli-germ cell in terface also un dergo rapid remodeli ng, invo Iving disassembly and reassembly of cell j un ctions, which, in turn, are supported by actin- and microtubule-based cytoskeletal remodeling. Interestingly, the underlying mechanism(s) and the invoIving biomolecule(s) that regulate or support cytoskeletal remodeling remain largely unknown. Herein, we used an in vitro model of primary Sertoli cell cultures that mimicked the Sertoli BTB in vivo overexpressed with the ribosomal protei n S6 (rpS6, the down stream signali ng protein of mammalian target of rapamycin complex 1 [mTORCl]) cloned into the mammalian expression vector pCI-neo, namely, quadruple phosphomimetic and constitutively active mutant of rpS6 (pCI-neo/p-rpS6-MT) versus pCI-neo/rpS6-WT (wild-type) and empty vector (pCI-neo/Ctrl) for studies. These findings provide compelling evidence that the mT0RCl/rpS6 signal pathway exerted its effects to promote Sertoli cell BTB remodeling. This was mediated through changes in the organization of actin- and microtubulebased cytoskeletons, involving changes in the distribution and/or spatial expression of actin- and microtubule-regulatory proteins. | Lin-Xi Li Si-Wen Wu Ming Yan Qing-Quan Lian Ren-Shan Ge C Yan Cheng | 2019 | Asian Journal of Andrology2019，21，4： | 4 |
| 2 | Biosynthesis of Unsaturated Fatty Acids via Mortierella Isabellina Cultivated in a Medium Containing Butanol显示文摘Mortierella isabellina was found to accumulate large amounts of unsaturated fatty acids when it was grown in a medium containing butanol(5 g/L) and yeast extract(10 g/L) and cultivated at 25 ℃ for 5 d during which additional feeding butanol of 2 g/L was fed after being cultivated for 48 h . The resultant mycelial lipids accounted for 40 1% of the dry mycelia, while about 34% of butanol in the medium was converted. The mycelial lipids contained four kinds of unsaturated fatty acids, i.e ., palmitoleic(4 9%), oleic(54 1%), linoleic(10 4%) and linolenic(5 4%) acids. Those accounted for 74 8% of the total fatty acids. The effects of the culture conditions, such as cultivation temperature, initial pH of the medium and additional feeding butanol in the course of cultivation, on the production of mycelial lipids by M. isabellina were studied. | XIAN Mo 1, YAN Ji chang 2, CHENG Tie xin 1, KANG Yi jian 3, LI Wen xing 1, BI Ying li 1 and ZHEN Kai ji 1** 1. College of Chemistry, Jilin University, Changchun 130023, P. R. China 2. College of Chemistry, Northeast Normal University, C | 2002 | Chemical Research in Chinese Universities2002，18，3： | 3 |
| 3 | Plastins regulate ectoplasmic specialization via its actin bundling activity on microfilaments in the rat testis显示文摘Plastins 是在哺乳动物的房间为交叉结合肌动朊微细丝所知的肌动朊绑定蛋白质(ABP ) 的一个家庭,创造必要的肌动朊微细丝捆授与房间极性和房间形状。Plastins 也在环境响应变化支持房间运动,在房间 / 织物生长和开发包含了。他们也响应感染或另外的病理学的条件授与粘性到房间和纸巾(例如,发炎) 。在睾丸,房间房间对在血睾丸障碍(BTB ) 并且在 Sertoli-spermatid 在 Sertoli 房间房间接口被发现的睾丸唯一的抛锚的连接(例如,在老鼠睾丸的 8-19 spermatids ) 基础并且顶端的 ectoplasmic 专门化(ES ) 分别地。ES 是抛锚的 F-actin-rich 最尤其是肌动朊微细丝捆组成的连接。3 关于由 plastin, 3 调整肌动朊微细丝的地位的机制产出一些深刻信息的用明确地将 plastin 击倒的 RNAi 的一份最近的报告经由它的内在的肌动朊细丝捆绑活动在 ES 绑。此处,我们考虑到这份报告在睾丸在 plastins 的角色上提供简短评论,它和这块地里的最近的调查结果,我们建议由哪个 plastins 在老鼠睾丸在肌动朊微细丝组织上经由他们的内在的活动在精子发生的上皮的周期期间调整 ES 功能的一个可能的模型。 | Nan Li Chris KC Wong C Yan Cheng | 2016 | Asian Journal of Andrology2016，18，5： | 3 |
| 4 | Circulating microRNAs as mirrors of acute coronary syndromes : MiR- acle or quagmire? 显示文摘 | Jin Li A Jiahong Xu C Yan Cheng al et | 2013 | J Cell Mol Med2013，17，11： | 1 |
| 5 | The role of TNF-alpha and its receptors in the production of Src-suppressed C kinase substrate by rat primary type-2 astrocytes 显示文摘 | Yan M Xia C Cheng C | | Brain Research0，1184，： | 1 |
| 6 | 查看详情显示文摘 | Yan L W Duan X R Ding X T Dong J Q Yang Q W Liu Y Zou X L Liu D Q Xuan W M Chen L Y Rao J Song X M Huang Y Mao W C Wang Q M Li Q Cao Z Li B Cao J Y Lei G J Zhang J H Li X D Chen W Cheng J Cui C H Cui Z Y Deng Z C Dong Y B Feng B B Gao Q D Han X Y Hong W Y | | 0，，： | 1 |
| 7 | Benefits of information sharing with supply chain partnerships 显示文摘 | Yu Z Yan H Cheng T C | 2001 | Industrial Management & Data System2001，101，3： | 1 |
| 8 | Optimal production stopping and restarting times for an EOQ model with deteriorating items 显示文摘 | Yan H Cheng T C E | 1998 | Journal of Operational Research Society1998，49，6： | 1 |
| 9 | Modeling the benefits of information sharing-based partnerships in a two-level supply chain显示文摘 | Yu Z Yan H Cheng T C E | 2002 | Journal of the Operational Research Society2002，53，4： | 1 |
| 10 | Development and genetic mapping of microsatellite markers from genome survey sequences in Brassica napus显示文摘 | Cheng X M Xu J S Xia S Gu J X Yan Y Fu J Qian X Q Zhang S C Wu J S Liu K D | | 0，，6： | 1 |
| 11 | Carbon nanotubes promote Cr (VI) reduction by alginate-immobilized Shewanella oneidensis MR-1 显示文摘 | Yan F F Wu C Cheng Y Y | 2013 | Biochemical Engineering Journal2013，77，： | 1 |
| 12 | Exponential Synchronization of A Class of Neural Networks With Time-Varying Delays 显示文摘 | CHENG C LIAO T YAN J | 2006 | IEEE Trans Syst Man and Cybernetics Part B : Cybem2006，36，1： | 1 |
| 13 | Optimal production stopping and restarting times for an EOQ model with deteriorating items显示文摘 | Yan H Cheng T C E | 1998 | Jounal of Operational Research Society1998，49，： | 1 |
| 14 | Experimental study on coalpyrolysis to acetylene in thermal plasma reactors 显示文摘 | Yan B H Xu P C Guo Y Jin Y Cheng Y | 2012 | ChemicalEngineering Journal2012，，： | 1 |
| 15 | New kinetic model of emulsifier - free emulsion polymerization 显示文摘 | Yan C E Cheng S Y Feng L X | 2000 | Polymerization Macromol Syrup2000，150，： | 1 |
| 16 | Kinetic and mechanism of emulsifier- free emulsion cnpnlymerization: Styrene - methyl methacrylate- acrylic acid system 显示文摘 | Yan C E Cheng S Y Feng L X | 1999 | J Pnlym Sci Part A1999，37，： | 1 |
| 17 | Globally exponential stability condition of a class of neural networks with time-varying delays 显示文摘 | LIAO T YAN J CHENG C | 2005 | Phys Lett A2005，338，： | 1 |
| 18 | The role of TNF-alpha and its receptors in the production of Src-suppressed C kinase substrate by rat primary type-2 astrocytes显示文摘 | Yan M Xia C Cheng C | 2007 | Brain Res2007，1184，1： | 1 |
| 19 | High critical current density in Ti-doped MgB2/Ta/Cu tape by powder-in-tube process显示文摘 | Fu B Q Feng Y Yan G Zhao Y Pradhan A K Cheng C H Ji P Liu X H Liu C F Zhou L Yau K F | | 0，，： | 1 |
| 20 | Long non-coding RNA HOTAIR promotes cell migration and invasion via down-regulation of RNAbinding motif protein 38 in hepatncellular carcinoma cells显示文摘 | Ding C Cheng S Yan$ Z | 2014 | Int J Mol Sci2014，15，3： | 1 |
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