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| 1 | Gene expression alteration during redox-dependent enhancement of arsenic cytotoxicity by emodin in HeLa cells显示文摘Emodin (1,3,8-trihydroxy-6-methylanthraquinone) could enhance the sensitivity of tumor cells to arsenic trioxide(As2O3)–induced apoptosis via generation of ROS, but the molecular mechanism has not been elucidated. Here, wecarried out cDNA microarray-based global transcription profiling of HeLa cells in response to As2O3/emodin cotreatment,comparing with As2O3–only treatment. The results showed that the expression of a number of genes was substantiallyaltered at two time points. These genes are involved in different aspects of cell function. In addition to redox regulationand apoptosis, ROS affect genes encoding proteins associated with cell signaling, organelle functions, cell cycle,cytoskeleton, etc. These data suggest that based on the cytotoxicity of As2O3, emodin mobilize every genomic resourcethrough which the As2O3–induced apoptosis is facilitated. | Xiao Jing WANG Jie YANG Hui CANG Yan Qiong ZOU Jing YI | 2005 | Cell Research2005，15，7： | 28 |
| 2 | p16 gene methylation in colorectal cancers associated with Duke's staging显示文摘AIM To explore the association of methylation of the CpG island in the promotor of the p16 tumor suppressor gene with the clinicopathological characteristics of the colorectal cancers.``METHODS Methylation-specific PCR (MSP) was used to detect p16 methylation of 62 sporadic colorectal cancer specimens.``RESULTS pi6 methylation was detected in 42% of the tumors. Dukes staging was associated with p16methylation status, p16 methylation occurred more frequently in Dukes C and D patients (75.9%) than in Dukes A and B patients (12.1%).``CONCLUSION p16 rnethylation plays a role in the carcinogenesis of a subset of colorectal cancer, and it might be linked to poor prognosis. | Jing Yi~1 Zhi-Wei Wang~1 Hui Cang~1 Yu-Ying Chen~1 Ren Zhao~2 Bao-Ming Yu~2 Xue-Ming Tang~1 1 Department of Cell Biology,2 Department of Surgery,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China | 2001 | World Journal of Gastroenterology2001，7，5： | 21 |
| 3 | Sevoflurane exposure in 7-day-old rats affects neurogenesis, neurodegeneration and neurocognitive function显示文摘Objective Sevoflurane is widely used in pediatric anesthesia and former studies showed that it causes neurodegeneration in the developing brain. The present study was carried out to investigate the effects of sevoflurane on neurogenesis, neurodegeneration and behavior. Methods We administered 5-bromodeoxyuridine, an S-phase marker, before, during, and after 4 h of sevoflurane given to rats on postnatal day 7 to assess dentate gyrus progenitor proliferation and Fluoro-Jade staining for degeneration. Spatial reference memory was tested 2 and 6 weeks after anesthesia. Results Sevoflurane decreased progenitor proliferation and increased cell death until at least 4 days after anesthesia. Spatial reference memory was not affected at 2 weeks but was affected at 6 weeks after sevoflurane administration. Conclusion Sevoflurane reduces neurogenesis and increases the death of progenitor cells in developing brain. This might mediate the lateonset neurocognitive outcome after sevoflurane application. | Fang Fang Zhanggang Xue Jing Cang | 2012 | Neuroscience Bulletin2012，28，5： | 12 |
| 4 | Knockdown of HMGB1 improves apoptosis and suppresses proliferation and invasion of glioma cells显示文摘Background: Effective methods for managing patients with solitary pulmonary nodules(SPNs) depend critically on the predictive probability of malignancy.Methods: Between July 2009 and June 2011, data on gender, age, cancer history, tumor familial history, smoking status, tumor location, nodule size, spiculation, calcification, the tumor border, and the final pathological diagnosis were collected retrospectively from 154 surgical patients with an SPN measuring 3-30 mm. Each final diagnosis was compared with the probability calculated by three predicted models—the Mayo, VA, and Peking University(PU) models. The accuracy of each model was assessed using area under the receiver operating characteristics(ROC) and calibration curves.Results: The area under the ROC curve of the PU model [0.800; 95% confidence interval(CI): 0.708-0.891] was higher than that of the Mayo model(0.753; 95% CI: 0.650-0.857) or VA model(0.728; 95% CI: 0.623-0.833); however, this finding was not statistically significant. To varying degrees, calibration curves showed that all three models overestimated malignancy.Conclusions: The three predicted models have similar accuracy for prediction of SPN malignancy, although the accuracy is not sufficient. For Chinese patients, the PU model may has greater predictive power.Background: Here, we introduced our short experience on the application of a new CUSA Excel ultrasonic aspiration system, which was provided by Integra Lifesciences corporation, in skull base meningiomas resection.Methods: Ten patients with anterior, middle skull base and sphenoid ridge meningioma were operated using the CUSA Excel ultrasonic aspiration system at the Neurosurgery Department of Shanghai Huashan Hospital from August 2014 to October 2014. There were six male and four female patients, aged from 38 to 61 years old(the mean age was 48.5 years old). Five cases with tumor located at anterior skull base, three cases with tumor on middle skull base, and two cases with tumor on sphenoid ridge.Results: All the patents received total resection of meningiomas with the help of this new tool, and the critical brain vessels and nerves were preserved during operations. All the patients recovered well after operation.Conclusions: This new CUSA Excel ultrasonic aspiration system has the advantage of preserving vital brain arteries and cranial nerves during skull base meningioma resection, which is very important for skull base tumor operations. This key step would ensure a well prognosis for patients. We hope the neurosurgeons would benefit from this kind of technique.Background: The purposes of this study were to explore the effects of high mobility group protein box 1(HMGB1) gene on the growth, proliferation, apoptosis, invasion, and metastasis of glioma cells, with an attempt to provide potential therapeutic targets for the treatment of glioma. Methods: The expressions of HMGB1 in glioma cells(U251, U-87 MG and LN-18) and one control cell line(SVG p12) were detected by real time PCR and Western blotting, respectively. Then, the effects of HMGB1 on the biological behaviors of glioma cells were detected: the expression of HMGB1 in human glioma cell lines U251 and U-87 MG were suppressed using RNAi technique, then the influences of HMGB1 on the viability, cycle, apoptosis, and invasion abilities of U251 and U-87 MG cells were analyzed using in a Transwell invasion chamber. Also, the effects of HMGB1 on the expressions of cyclin D1, Bax, Bcl-2, and MMP 9 were detected. Results: As shown by real-time PCR and Western blotting, the expression of HMGB1 significantly increased in glioma cells(U251, U-87 MG, and LN-18) in comparison with the control cell line(SVG p12); the vitality, proliferation and invasive capabilities of U251 and U-87 MG cells in the HMGB1 siR NA-transfected group were significantly lower than those in the blank control group and negative control(NC) siR NA group(P<0.05) but showed no significant difference between the blank control group and NC siR NA group. The percentage of apoptotic U251 and U-87 MG cells was significantly higher in the HMGB1 siR NA-transfected group than in the blank control group and NC siR NA group(P<0.05) but was similar between the latter two groups. The HMGB1 siR NA-transfected group had significantly lower expression levels of Cyclin D1, Bcl-2, and MMP-9 protein in U251 and U-87 MG cells and significantly higher expression of Bax protein than in the blank control group and NC siR NA group(P<0.05); the expression profiles of cyclin D1, Bax, Bcl-2, and MMP 9 showed no significant change in both blank control group and NC siR NA group. Conclusions: HMGB1 gene may promote the proliferation and migration of glioma cells and suppress its effects of apoptosis. Inhibition of the expression of HMGB1 gene can suppress the proliferation and migration of glioma cells and promote their apoptosis. Our observations provided a new target for intervention and treatment of glioma. | Jing Zhang Cang Liu Ruiguang Hou | 2014 | Chinese Journal of Cancer Research2014，26，6： | 9 |
| 5 | The biphasic redox sensing of SENP3 accounts for the HIF-1 transcriptional activity shift by oxidative stress显示文摘目的: 在氧化应力的不同层次下面的 transcriptional 活动由反应氧化种类(ROS ) 引起了调查位于组织缺氧可诱导的 factor-1 (HIF-1 ) 的 biphasic 氧化还原作用规定下面的机制。 | Ying WANG Jie YANG Kai YANG Hui CANG Xin-zhi HUANG Hui LI Jing YI | 2012 | Acta Pharmacologica Sinica2012，33，7： | 8 |
| 6 | Oxidative modification of caspase-9 facilitates its activation via disulfide-mediated interaction with Apaf-1显示文摘细胞内部的反应的氧种类(ROS ) 被知道调整 apoptosis。caspase-9 的激活,在 mitochondrial apoptotic 串联的起始的 caspase,仔细与被联系 ROS,而是它是不清楚的是否 ROS 经由直接氧化的修正调整 caspase-9。阐明的现在的学习目的由哪个 ROS 调停的分子的机制 caspase-9 激活。我们的结果证明细胞的氧化状态便于 caspase-9 激活。氢过氧化物治疗引起 caspase-9 和 apoptosis 的激活,并且支持在 caspase-9 和 apoptotic 之间的一个相互作用激活朊酶的因素 1 (Apaf-1 ) 经由二硫化物形成。另外,在里面一在里面 vitro 没有线粒体的系统, thiol 氧化剂 diamide 由便于连接二硫化物的建筑群的形成支持 caspase-9 和 caspase-9/Apaf-1 相互作用的汽车劈开。最后,在 caspase-9 的 C403 的一个点变化通过二硫化物形成的废除损害有 Apaf-1 的两支持 H2O2 的 caspase-9 激活和相互作用。在细胞色素 c 和 C403S 异种之间的协会在细胞色素 c 和野类型的 caspase-9 之间是比那显著地弱的,显示 caspase-9 的那氧化修正贡献在氧化应力下面的 apoptosome 形成。一起拿,由 ROS 的 caspase-9 的氧化修正能调停它和 Apaf-1 的相互作用,和罐头因此支持它的汽车劈开和激活。这机制可以在氧化应力下面便于 apoptosome 形成和 caspase-9 激活。 | Yong Zuo Binggang Xiang Jie Yang Xuxu Sun Yumei Wang Hui Cang Jing Yi | 2009 | Cell Research2009，19，4： | 6 |
| 7 | Two-dimensional Electrophoresis Analysis of Proteins in Response to Cold Stress in Extremely Cold-resistant Winter Wheat Dongnongdongmai 1 Tillering Nodes显示文摘The overwintering survival ratio of the cultivar Dongnongdongmai 1 with strong cold-resistance in paramos of Heilongjiang Province in China are over 85%. The tillering nodes are the most important organs for overwintering survival of winter wheat, because there are more substances associated with cold resistance in tillering nodes than those in leaves and roots. Proteins in the tillering nodes of the cold-resistant cultivar Dongnongdongmai 1 grown under field conditions with or without any lowtemperature stress were analyzed by 2-dimensional electrophoresis and identified by mass spectrometry. In the range of pH 4-7, the expression of 37 proteins showed obvious difference (±more than two fold) in the proteomic maps of cold-stressed and non-stressed tillering nodes, including a new protein spot. All proteins exhibiting the difference in expression were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, followed by a database search for protein identification and function prediction. Five groups of proteins were confirmed, namely stress-related proteins (22%), metabolism-associated proteins (35%), and signaling molecules (24%), cell wall-binding proteins (5%), unclear proteins (14%). This indicated that tillering node cells supported the energy requirements of plant growth and stress resistance by signal transduction adapting to metabolism and structure. | Cang Jing Yu Jing Liu Li-jie Yang Yang Cui Hong Hao Zai-bin Li Zhuo-fu | 2012 | Journal of Northeast Agricultural University(English Edition)2012，19，1： | 2 |
| 8 | Protective effect of melatonin on reexpansion pulmonary edema in rats model显示文摘 | ZHONG Ming XUE Zhang-gang CANG Jing WU Wei HUANG Jun-feng ZHU Du-ming | 2013 | Chinese Medical Journal2013，，3： | 1 |
| 9 | Dry powder inhalations containing thymopentin and its immunomodulating effects in Wistar rats显示文摘 | GAO Jing DING Xue-ying CHU Cang | 2009 | Eur J Pharm Sci2009，36，4： | 1 |
| 10 | Effect of Intraoperative Amino Acid Infusion on Blood Glucose under General Anesthesia Combined with Epidural Block显示文摘 | Zhong Jing Ge Sheng Jin Zhuang Xiao Feng Cang Jing Xue Zhang Gang | 2012 | Annals of Nutrition & Metabolism2012，，1： | 1 |
| 11 | Progress in study steviol glycoside from Stevia rebaudiana Berton in Japan显示文摘 | HAO Zaibin QI Donglai CANG Jing | 2005 | China Biotechnology2005，25，： | 1 |
| 12 | Sevoflurane/propofol coadministration provides better recovery than sevoflurane in combined general/epidural anesthesia: a randomized clinical trial显示文摘 | Chao Liang Ming Ding Fang Du Jing Cang Zhanggang Xue | 2014 | Journal of Anesthesia2014，，5： | 1 |
| 13 | Anatomical Structure Comparison Between Leaves of Two Winter Wheat Cultivars with Different Cold/Freezing Tolerance Under Low Temperature Stress显示文摘Winter wheat (Triticum aestivum) cultivars Dongnongdongmai 1 with strong cold/freezing tolerance and Jimai 22 with weak cold/freezing tolerance were used for investigating the difference of microstructure and ultrastructure between leaves of two cultivars under low temperature stress (5℃ and -15℃) using optical and electron microscope. The results showed that there was no obvious difference between leaves of Dongnongdongmai 1 and Jimai 22 in microstructure. However, the difference between those leaves was distinct in ultrastructure. The grana lamella and stroma lamella were stacked regularly and arranged parallelly along the long axis of chloroplasts in cv. Dongnongdongmai 1, while the arrangement directions of thylakoids in Jimai 22's leaves were so irregular as to form various angles with the long axis of chloroplasts. At -15℃, the mitochondrias were swelled to be round and the structure of cristaes became blurry in both cultivars' leaves, while some cristaes of Jimai 22 disappeared. These results would provide theoretical evidence for selecting cold/freezing tolerant winter wheat germplasm resources. | YU Jing CANG Jing ZHOU Zishan LIU Lijie | 2011 | Journal of Northeast Agricultural University(English Edition)2011，18，3： | 1 |
| 14 | 极长链酰基辅酶A脱氢酶缺乏症ACADVL基因新发突变导致线粒体功能障碍显示文摘目的:鉴定并阐述6个ACADVL新发突变的对细胞和分子功能的影响,为后期分子诊断和临床病例管理提供科学依据。创新点:本文从分子功能和结构的角度阐述6个新位点对蛋白和细胞功能的不同影响。方法:通过体外构建突变载体建立各突变的过表达细胞系,检测各细胞系脂肪酸代谢能力、线粒体呼吸链功能、线粒体产三磷酸腺苷(ATP)能力、活性氧(ROS)和凋亡水平。同时检测了新发突变对蛋白二聚体稳定性的影响并用分子动力学模拟了突变蛋白构像的改变。结论:突变(c.541C>T、c.863T>G、c.895A>G、c.1238T>C、c.1276G>A和c.1505T>A)过表达细胞系均表现出脂肪酸代谢障碍,线粒体呼吸链功能障碍,ATP产生水平下降和线粒体ROS水平上升。在无糖条件下,各细胞系的凋亡水平上升。各突变影响了极长链酰基辅酶A脱氢酶(VLCAD)蛋白二聚体的稳定性,分子动力学模拟预测每个突变均会导致VLCAD蛋白结构的发生重要构象变化。 | Ting CHEN Fan TONG Xiao-yu WU Ling ZHU Qiu-zi YI Jing ZHENG Ru-lai YANG Zheng-yan ZHAO Xiao-hui CANG Qiang SHU Ping-ping JlANG | 2020 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2020，21，11： | 1 |
| 15 | A Novel Method for Modification of SMPU by Micro-phase显示文摘A novel method of controlling the shape memory properties of shape memory polyurethane (SMPU) by addition of micro-phase separation promoters including 1-octadecanol (ODO) and liquid paraffin (LP) is reported. The results indicate that the strain recovery temperature and the strain modulus rate (Eg/Er) were increased significantly with addition of small amount of micro-phase separation promoters. Thus it can increase the shape memory fixity rate and other shape memory behaviors of SMPU. | Shao Jun CHEN Jing Cang SU Peng Sheng LIU | 2005 | Chinese Chemical Letters2005，16，9： | 1 |
| 16 | Detection of sugar accu- mulation and expression levels of correlative key enzymes in winter wheat (Triticum aestivum) at low temperatures显示文摘 | Zeng Yan Yu Jing Cang Jing | 2011 | Bioscience Bioteehnology and Biochemistry2011，75，4： | 1 |
| 17 | Dry powder inhalations containing thymopentin and its immunomodulating effects in Wistar rats显示文摘 | Jing Gao Xueying Ding Cang Chu Lei Lu Yi Zhang Yan Chen Wei Fan Guodong Li Shen Gao | 2008 | European Journal of Pharmaceutical Sciences2008，，： | 1 |
| 18 | Design of broadband circularly polarized dielectric resonator antenna using improved feed network显示文摘 | Rong‐Cang Han Shun‐Shi Zhong Jing Liu | 2014 | Microw Opt Technol Lett2014，，9： | 1 |
| 19 | DNA damage response-related immune activation signature predicts the response to immune checkpoint inhibitors: from gastrointestinal cancer analysis to pan-cancer validation显示文摘Objective: DNA damage response(DDR) deficiency has emerged as a prominent determinant of tumor immunogenicity. This study aimed to construct a DDR-related immune activation(DRIA) signature and evaluate the predictive accuracy of the DRIA signature for response to immune checkpoint inhibitor(ICI) therapy in gastrointestinal(GI) cancer.Methods: A DRIA signature was established based on two previously reported DNA damage immune response assays. Clinical and gene expression data from two published GI cancer cohorts were used to assess and validate the association between the DRIA score and response to ICI therapy. The predictive accuracy of the DRIA score was validated based on one ICI-treated melanoma and three pan-cancer published cohorts.Results: The DRIA signature includes three genes(CXCL10, IDO1, and IFI44L). In the discovery cancer cohort, DRIA-high patients with gastric cancer achieved a higher response rate to ICI therapy than DRIA-low patients(81.8% vs. 8.8%;P < 0.001), and the predictive accuracy of the DRIA score [area under the receiver operating characteristic curve(AUC) = 0.845] was superior to the predictive accuracy of PD-L1 expression, tumor mutational burden, microsatellite instability, and Epstein–Barr virus status. The validation cohort demonstrated that the DRIA score identified responders with microsatellite-stable colorectal and pancreatic adenocarcinoma who received dual PD-1 and CTLA-4 blockade with radiation therapy. Furthermore, the predictive performance of the DRIA score was shown to be robust through an extended validation in melanoma, urothelial cancer, and pan-cancer.Conclusions: The DRIA signature has superior and robust predictive accuracy for the efficacy of ICI therapy in GI cancer and pancancer, indicating that the DRIA signature may serve as a powerful biomarker for guiding ICI therapy decisions. | Junya Yan Shibo Wang Jing Zhang Qiangqiang Yuan Xianchun Gao Nannan Zhang Yan Pan Haohao Zhang Kun Liu Jun Yu Linbin Lu Hui Liu Xiaoliang Gao Sheng Zhao Wenyao Zhang Abudurousuli Reyila Yu Qi Qiujin Zhang Shundong Cang Yuanyuan Lu Yanglin Pan Yan Kong Yongzhan Nie | 2024 | Cancer Biology & Medicine2024，21，3： | 0 |
| 20 | Regulation of Floral Organ Identity in Arabidopsis by Ectopic Expression of OsMADS58显示文摘The ABCDE model has been described comprehensively to interpret the mechanism of floral organ development.In Arabidopsis,AGMAOUS was a well-studied class C gene encoding a transcription factor with MADS box,and its overexpression in Arabidopsis induced the transformation of sepals to carpels and petals to stamens.However,there is little knowledge about the function of OsMADS58,which was a homologue of AGMAOUS in rice.In our study,OsMADS58 driven by the UBIQUITIN promoter was ectopicly expressed in Arabidopsis.,and carpel-like sepals,incomplete petals and stamens instead of petals were found in different transgenic plants according to different OsMADS58 mRNA expression levels.The phenotype of transgenic plant was similar to the AGMAOUS overexpression plant,suggesting that OsMADS58 might play an important role in flower development like AGMAOUS and they keep conserved function during evolution. | Wang Yan-mei Yan Da-wei Zhang Ying-ying Li Jing Cang Jing | 2012 | Journal of Northeast Agricultural University(English Edition)2012，19，3： | 0 |
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