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| 1 | Dynamic Expression of mi RNAs and Their Targets in the Response to Drought Stress of Grafted Cucumber Seedlings显示文摘Grafting of cucumber is widely used to improve growth and tolerance to biotic and abiotic stresses. Micro RNAs(miRNAs) regulate plant growth and development, and respond to various stresses through negative, post-transcriptional regulation of the expression of their target genes. We grafted cucumber(Cucumis sativus) scions onto pumpkin(Cucurbita moschata) rootstocks to study the molecular mechanisms of miRNA-mediated graftinginduced responses to drought stress. The relative expressions of 17 selected miRNAs and their predicted target m RNAs were detected by quantitative real-time PCR(q RT-PCR) in leaves and roots of hetero-grafted cucumber seedlings(cucumber as scion and pumpkin as rootstock) and autografted cucumber seedlings(cucumber as scion and rootstock) after 24 hours of 15% PEG6000 treatment. Compared with the expression in leaves of auto-grafted cucumber seedlings, the expressions of most miRNAs in the leaves of hetero-grafted seedlings changed dynamically: induced under normal conditions, and reduced after 3 h of drought stress, and then induced after 8 h and 24 h of drought stress. Similarly, compared with the expression in roots of auto-grafted cucumber seedlings, the expressions of most miRNAs in the roots of hetero-grafted cucumber seedlings changed dynamically: reduced under normal condition, and still reduced after 1 h, but induced after 3 h and 8 h, then reduced significant y after24 h of drought stress. These results are useful for the functional analysis of miRNAs in the mediation of grafting-dependent drought tolerance. | LI Chaohan LI Yansu BAI Longqiang HE Chaoxing YU Xianchang | 2016 | Horticultural Plant Journal2016，2，1： | 5 |
| 2 | Interference of CsGPA1,theα-submit of G protein,reduces drought tolerance in cucumber seedlings显示文摘The G proteinα-subunit,GPA1,is an integral component of several signaling pathways in plants,including response to abiotic stress.However,the molecular mechanism behind these processes remains largely unknown in the cucumber plant(Cucumis sativus L.).In order to further understand the role of CsGPA1 in cucumber under drought stress,changes in plant growth,physiological parameters,and gene expression of CsAQPs were all measured under water stress induced by polyethylene glycol(PEG)using wild type(WT)and CsGPA1-interference(RNAi)cucumber seedlings.Our results demonstrated that the RNAi plants had lower drought tolerance,displaying seriously withered leaves,lower relative growth rate,lower root-shoot ratio,and lower root activity under drought stress compared to WT plants.Physiological studies indicated that the suppression of CsGPA1 weakened drought stress tolerance due to higherwater loss rate in the leaves,higher levels of hydrogen peroxide(H2O2),increased malondialdehyde(MDA)content,lower free proline content,lower soluble sugar content,lower soluble protein content,and decreased antioxidant enzyme activities.qRT-PCR analysis demonstrated that the interference of CsGPA1 up-regulated the expression of most AQP genes(except for CsPIP2;3 in leaves)and down-regulated the expression of CsPIP1;2,CsPIP1;4,CsPIP2;1,and CsPIP2;4 in roots under drought stress when compared to WT plants.Our results demonstrated that CsGPA1 could function as a positive regulator in drought stress response by decreasing the accumulation of reactive oxygen species(ROS),improving permeable potentials,and reducing water loss in cucumber plants. | Yumei Liu Qinghua Du Longqiang Bai Mintao Sun Yansu Li Chaoxing He Jun Wang Xianchang Yu Yan Yan | 2021 | Horticultural Plant Journal2021，7，3： | 3 |
| 3 | Controlling memory effects of three-layer structured hybrid bistable devices based on graphene sheets sandwiched between two laminated polymer layers显示文摘 | Chaoxing Wu Fushan Li Tailiang Guo Tae Whan Kim | 2011 | Organic Electronics2011，，1： | 1 |
| 4 | Efficient Self-Assembled DNA Nanoparticles through Rolling Circle Amplification for siRNA Delivery in vitro显示文摘Effective and low toxicity delivery of siRNA is of great importance for clinical gene therapy. Herein, self-assembled DNA nanoparticles (NPs) based on rolling circle amplification (RCA) with a small interfering RNA (siRNA) payload were successfully developed as a facile and efficient siRNA delivery strategy. This intracellular gene silencing strategy exhibits various advantages including low toxicity, high efficiency, and good stability. The synthesized DNA NPs serve as siRNA carriers, protecting the siRNA against nuclease degradation. We demonstrate that the obtained self-assembled siRNA/NP/PEI system can successfully deliver enhanced green fluorescent protein (EGFP)-siRNA into HeLa cells, realizing the same EGFP knockdown efficiency with less toxicity as that of commercial Lipofectamine 2000. | Qian Yao Yuqi Chen Fan Wu1 Fan Wu Chaoxing Liu Tingting Hong Wei Li Yi Chen Xiang Zhou | 2019 | Chinese Journal of Chemistry2019，37，6： | 1 |
| 5 | Preparation of silica and titanium-containing silica hollow spheres atsupercritical CO2/H2O interface显示文摘 | Zhang Chaoxing Zhang Jianling Zhang Xiaogang | 2007 | Journal of Supercritical Fluids2007，42，： | 1 |
| 6 | Changes of antioxidative enzymes and cell membrane osmosis in tomato colonized by arbuscular mycorrhizae under NaCl stress显示文摘 | ZhongQun He ChaoXing He ZhiBin Zhang ZhiRong Zou HuaiSong Wang | 2007 | Colloids and Surfaces B: Biointerfaces2007，，2： | 1 |
| 7 | Controlling memory effects of three layer structured hybrid bistable devices based on graphene sheets sandwiched between two laminated polymer layers显示文摘 | Wu Chaoxing I5 Fushan Guo Tailiang | 2012 | ()rg Electr2012，13，17： | 1 |
| 8 | Surfacecharacteristicsofcrop-residue-derivedblackcarbonandlead(Ⅱ)adsorption显示文摘 | YUPQ HAIY CHAOX etal | 2008 | WaterRes2008，42，： | 1 |
| 9 | Subsolidus binary phase diagrams of C10ZnCl - C16ZnCl and C12ZnCl - C16ZnCl 显示文摘 | Wu Kezhong Zhang Chaoxing Li Yajuan | 2005 | Journal of the Chinese Chemical Society2005，52，： | 1 |
| 10 | Efficient source mask optimization using multipole source representation显示文摘 | Chaoxing Yang Sikun Li Xiangzhao Wang | 2014 | J Miero/Nanolith MEMS MOEMS2014，13，04： | 1 |
| 11 | Effects of organized soil cultivation on yield and quality of tomato in greenhouse显示文摘 | Zhang Zhibin He Chaoxing | 2005 | Acta Horticulturae2005，691，： | 1 |
| 12 | Neuroprotectiveeffectofostholeagainstoxygenandglucosedeprivationinratcorticalneurons:in-volvementofmitogen-activatedproteinkinasepathway显示文摘 | ChenT LiuW ChaoX etal | 2011 | Neuro-science2011，183，： | 1 |
| 13 | Internal architecture and mobility of tidal sand ridges in the East China Sea显示文摘 | Zhenxia Liu Serge Berné Yoshiki Saito Hua Yu Alain Trentesaux Katsuto Uehara Ping Yin J. Paul Liu Chaoxing Li Guanghai Hu Xiangqin Wang | 2007 | Continental Shelf Research2007，，13： | 1 |
| 14 | To- pological insulators in Bi2 Te3 , Bi2 Se3 and Sb2 Te3 with Dirac cone on the surface显示文摘 | LIU CHAOXING QI XIAOLIANG ZHANG SHOUCHENG | 2009 | Nature Physics2009，5，： | 1 |
| 15 | Neuroprotectiveeffectofostholeagainstacuteischemicstrokeonmiddlecerebralischemiaocclu-sioninrats显示文摘 | ChaoX ZhouJ ChenT etal | 2010 | BrainRes2010，1363，： | 1 |
| 16 | Field-emi-ssion performance and structural change mechanism ofmultiwalled carbon nanotubes by oxygen plasma treatment显示文摘 | Wu Chaoxing Li Fushan Zhang Yongai | 2013 | 94:48-522013，94，： | 1 |
| 17 | Influence of surfactants on the morphologies of CaCO3 by carbonation route with compressed CO2 显示文摘 | Chaoxing Zhang Jianling Zhang Xiaoying Feng | 2008 | Colloids and Surfaces A: Physicochem Eng Aspects2008，324，： | 1 |
| 18 | STEADY STATE RESPONSE ANALYSIS ON HELICAL SPRING IMPACTED BY SHORT WAVE显示文摘螺旋状的春天的波浪传播特性在波浪影响理论上被用于影响车辆的 establish2-DOF 模型。就螺旋状的春天的具体结构而言,在车辆的不同影响频率下面的相应回答被模仿。为什么的原因车辆地板在上在某特殊频率回答地基于分析回答被探索。当装紧在低频率时,春天的变化没被考虑,但是这不影响结果。因为速度和加速的传播人物在螺旋状的春天是一致的,速度和加速的回答人物也是一致的,唯一的差别是大小,它能使用加速回答分析速度回答。 | FU Chaoxing WANG Xiulun WANG Jirong LIU Dawei | 2006 | Chinese Journal of Mechanical Engineering2006，19，4： | 0 |
| 19 | Multifaceted regulatory functions of CsBPC2 in cucumber under salt stress conditions显示文摘BASIC PENTACYSTEINE(BPC)transcription factors are essential regulators of plant growth and development.However,BPC functions and the related molecular mechanisms during cucumber(Cucumis sativus L.)responses to abiotic stresses,especially salt stress,remain unknown.We previously determined that salt stress induces CsBPC expression in cucumber.In this study,Csbpc2 transgene-free cucumber plants were created using a CRISPR/Cas9-mediated editing system to explore CsBPC functions associated with the salt stress response.The Csbpc2 mutants had a hypersensitive phenotype,with increased leaf chlorosis,decreased biomass,and increased malondialdehyde and electrolytic leakage levels under salt stress conditions.Additionally,a mutated CsBPC2 resulted in decreased proline and soluble sugar contents and antioxidant enzyme activities,which led to the accumulation of hydrogen peroxide and superoxide radicals.Furthermore,the mutation to CsBPC2 inhibited salinity-induced PM-H+-ATPase and V-H+-ATPase activities,resulting in decreased Na+efflux and increased K+efflux.These findings suggest that CsBPC2 may mediate plant salt stress resistance through its effects on osmoregulation,reactive oxygen species scavenging,and ion homeostasis-related regulatory pathways.However,CsBPC2 also affected ABA signaling.The mutation to CsBPC2 adversely affected salt-induced ABA biosynthesis and the expression of ABA signaling-related genes.Our results indicate that CsBPC2 may enhance the cucumber response to salt stress.It may also function as an important regulator of ABA biosynthesis and signal transduction.These findings will enrich our understanding of the biological functions of BPCs,especially their roles in abiotic stress responses,thereby providing the theoretical basis for improving crop salt tolerance. | Shuzhen Li Mintao Sun Li Miao Qinghua Di Lijun Lv Xianchang Yu Yan Yan Chaoxing He Jun Wang Aokun Shi Yansu Li | 2023 | Horticulture Research2023，10，5： | 0 |
| 20 | 5-Formyluracil targeted biochemical reactions with proteins inhibit DNA replication,induce mutations and interference gene expression in living cells显示文摘Covalent DNA–protein cross-links are toxic DNA lesions that interfere with essential biological processes,which can cause serious biological consequences,such as genomic instability and protein misexpression.5-Formyluracil(5 fU) as an important modification in DNA,which is mainly from oxidative damage,exists in a variety of cells and tissues.We have reported that 5 fU mediated DNA–protein conjugates could exist in human cells [Zhou et al.CCS Chem.2(2020) 54–63].We now aimed to explore its potential biological effects in vitro and in vivo.In this paper,we firstly reported that 5 fU intermediated DNA–peptide or DNA–protein conjugates(both were called DPCs) could inhibit different polymerases bypass or cause mutations.Then we further investigated the functional impacts caused by 5 fU-mediated DPCs,which appeared in different gene expression components [in the promoter sequence or 50-untranslated regions(UTR)].These results together may contribute to a broader understanding of DNA–protein interactions as well as the biological functions associated with 5 fU. | Guangrong Zou Kaiyuan Zhang Wei Yang Chaoxing Liu Zhentian Fang Xiang Zhou | 2021 | Chinese Chemical Letters2021，32，10： | 0 |
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