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| 1 | Effect of Fanbaicao(Herba Potentillae Discoloris) oil on the expression of p21 and CDK4 in HepG2 cells显示文摘OBJECTIVE:To research the anti-cancer mechanism of the Traditional Chinese Medicine Fanbaicao(Herba Potentillae Discoloris) oil in the human hepatoma cell line Hep G2.METHODS:Gas chromatography was used to analyze the components of Fanbaicao(Herba Potentillae Discoloris).We tested the inhibitory effect of Fanbaicao(Herba Potentillae Discoloris) oil on the human hepatoma cell line Hep G2 in vitro using 3-(4,5-Dimet hylt hiazol-2-yl)-2,5-dip henyltetrazoliumbromide assays.Fluorescence activating cell sorter analysis was used to examine the levels of apoptosis,and western blot and immunofluorescence were used to detect the expression of p21,p-p21 and CDK4 proteins.RESULTS:Fanbaicao(Herba Potentillae Discoloris)oil contains 45 ingredients,and L-ascorbic acid 2,6-bispalmitate was the main component and accounted for 44.96% of total drive-off peak area.Other components included(Z)-14-met hyl-8-exadecenal-acetal(8.56%),phytol(7.74%) and lauric acid(6.31%).Fanbaicao(Herba Potentillae Discoloris)oil treatment reduced the proliferation of Hep G2 cells and the half growth inhibition concentration(IC50) was 2.03 mg/m L.Furthermore,we also observed significantly increased Hep G2 cell apoptosis in a dose-dependent manner(P < 0.05).Fanbaicao(Herba Potentillae Discoloris) oil significantly increased the expression of p21 and p-p21 and significantly decreased the expression of CDK4 in Hep G2 cells compared with controls(P < 0.01).CONCLUSION:Our results showed that Fanbaicao(Herba Potentillae Discoloris) oil has anti-cancer activities in Hep G2 cells,which is probably related to the upregulation of p21 and p-p21 and downregulation of CDK4 expression. | Liu Lei Chen Guang Wang Baixin Chen Liping Wang Shuqiu Liu Zhixin Ma Xiaoru Wang Fangfang Liang Yanfeng Wu Jiamei Yang Zhiwei | 2016 | Journal of Traditional Chinese Medicine2016，36，4： | 1 |
| 2 | The breakup and atomization of a viscous liquid jet 显示文摘 | YI SHIJUN XIE MAOZHAO CHEN BAIXIN | 1996 | ACTA Mechanica SINICA (English Series)1996，12，2： | 1 |
| 3 | The breakup and atomization of a viscous Liquid Jet 显示文摘 | Yi Shijun Xie Maozhao Chen Baixin | 1996 | ACTA MECHANICA SINICA1996，12，： | 1 |
| 4 | The Breakup and Atomization of a Viscous Liquid Jet显示文摘 | Yi Shijun Xie Maozhao Chen Baixin | 1996 | ACTA Mechanica SINICA (English Series)1996，12，2： | 1 |
| 5 | The breakup and atomization of a viscous liquid jet显示文摘 | Yi Shijun Xie Maozhao Chen Baixin | 1996 | ACTA Mechanica SINICA (English Series)1996，12，2： | 1 |
| 6 | The breakup and atomization of a viscous liquid jet显示文摘 | Yi Shijun Xie Maozhao Chen Baixin | 1996 | ACTA Mechanica SINICA ( English Series)1996，12，2： | 1 |
| 7 | FORMATION OF HEXAGONAL Co_(2—3)C IN Co BY CARBON ION IMPLANTATION显示文摘Co thin films were subjected to 50 keV carbon ion implantation.At the dose of 2.5×1017/cm2,a hexagonal Co-carbide phase was observed for the first time.The lattice con-stants from electron diffraction are a=0.2685 nm and c=0.4335 nm.The phase does not dis-appear until the dose of 9×1017/cm2.Auger spectra showed that the stoichiometry wasCo2-3C.The behavior of the ferromagnetic carbides along the descending sequence ofNi-Fe-Co by Fermi energy of solids was interpreted.Furthermore,based on the kinetic con-dition of phase transformation and the band theory of solids,a possible explanation about thedifference of the results of ion-metallurgy and thermal metallurgy was proposed. | WANG Jian WU Xingfang CHEN Xunping CAI Jun University of Science and Technology Beijing,Beijing,China LIU Baixin Tsinghua University,Beijing,China FANG Zhengzhi Beijing Institute of Space Physics,Academia Sinica,Beijing,China | 1992 | Acta Metallurgica Sinica(English Letters)1992，5，2： | 1 |
| 8 | The Breakup and Atomization of a Viscous Liquid Jet显示文摘 | Yi Shijun Xie Maozhao Chen Baixin | 1996 | ACTA Mechanica Sinica (English Series)1996，12，2： | 1 |
| 9 | Using 137Cs fingerprint technique to estimate sediment deposition and erosion rates from Yongkang depression in karst region of Southwest China显示文摘 | Yah Baixin Zhang Xinbao Chen Hongsong | 2010 | Land Degrad Dev2010，21，： | 1 |
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