|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A structural view of the antibiotic degradation enzyme NDM-1 from a superbug显示文摘Gram-negative Enterobacteriaceae with resistance to carbapenem conferred by New Delhi metallo-β-lactamase 1(NDM-1)are a type of newly discovered antibioticresistant bacteria.The rapid pandemic spread of NDM-1 bacteria worldwide(spreading to India,Pakistan,Europe,America,and Chinese Taiwan)in less than 2 months characterizes these microbes as a potentially major global health problem.The drug resistance of NDM-1 bacteria is largely due to plasmids containing the blaNDM-1 gene shuttling through bacterial populations.The NDM-1 enzyme encoded by the blaNDM-1 gene hydrolyzes β-lactam antibiotics,allowing the bacteria to escape the action of antibiotics.Although the biological functions and structural features of NDM-1 have been proposed according to results from functional and structural investigation of its homologues,the precise molecular characteristics and mechanism of action of NDM-1 have not been clarified.Here,we report the threedimensional structure of NDM-1 with two catalytic zinc ions in its active site.Biological and mass spectroscopy results revealed that D-captopril can effectively inhibit the enzymatic activity of NDM-1 by binding to its active site with high binding affinity.The unique features concerning the primary sequence and structural conformation of the active site distinguish NDM-1 from other reported metallo-β-lactamases(MBLs)and implicate its role in wide spectrum drug resistance.We also discuss the molecular mechanism of NDM-1 action and its essential role in the pandemic of drug-resistant NDM-1 bacteria.Our results will provide helpful information for future drug discovery targeting drug resistance caused by NDM-1 and related metallo-β-lactamases. | Yu Guo Jing Wang Guojun Niu Wenqing Shui Yuna Sun Honggang Zhou Yaozhou Zhang Cheng Yang Zhiyong Lou Zihe Rao | 2011 | Protein & Cell2011，2，5： | 16 |
| 2 | Deep structure at northern margin of Tarim Basin显示文摘In this paper, a 2D velocity structure of the crust and the upper mantle of the northern margin of the Tarim Basin (TB) has been obtained by ray tracing and theoretical seismogram calculation under the condition of 2D lateral inhomogeneous medium using the data of seismic wide angle reflection/refraction profile from Baicheng to Da Qaidam crossing the Kuqa Depression (KD) and Tabei Uplift (TU). And along the Baicheng to Da Qaidam profile, 4 of the 10 shot points are located in the northern margin of the TB. The results show that the character of the crust is uniform on the whole between the KD and TU, but the depth of the layers, thickness of the crust and the velocity obviously vary along the profile. Thereinto, the variation of the crust thickness mainly occurs in the middle and lower crust. The Moho has an uplifting trend near the Baicheng shot point in KD and Luntai shot point in TU, and the thickness of the crust reduces to 42 km and 47 km in these two areas, respectively. The transition zone between the KD and TU has a thickest crust, up to 52 km. In this transition zone, there are high velocity anoma-lies in the upper crust, and low velocity anomalies in the lower crust, these velocity anomalies zone is near vertical, and the sediment above them is thicker than the other areas. According to the velocity distributions, the profile can be divided into three sections:KD, TU and transition zone between them. Each section has a special velocity structural feature, the form of the crystalline basement and the relationship between the deep structure and the shallow one. The differences of velocity and tectonic between eastern and western profile in the northern margin of the Tarim Basin (NMTB) may suggest different speed and intensity of the subduction from the Tarim basin to the Tianshan orogenic belt (TOB). | ZHAO JunMeng CHENG HongGang PEI ShunPing LIU HongBing ZHANG JianShi LIU BaoFeng | 2008 | Chinese Science Bulletin2008，53，10： | 10 |
| 3 | Surface curvature-confined strategy to ultrasmall nickel-molybdenum sulfide nanoflakes for highly efficient deep hydrodesulfurization显示文摘Size-controlled synthesis of two-dimensional(2D)catalysts with low stacking numbers and small nanoflake lengths is crucial for promoting the catalytic performance in diverse heterogeneous catalysis.Herein,we report a facile and general“surface curvature-confined synthesis”strategy to modulate the slab lengths and stacking numbers of 2D transition metal sulfides by controlling the strain induced by different surface curvature of supports.An efficient NiMo sulfide with shorter slab length(average 3.71 nm),less stacking number(1–2 layers)and more edge active sites is synthesized onto ZSM-5 zeolites with the average size of 100 nm,which shows superior kHDS value of dibenzothiophene(14.05×10^−7 mol/(g·s)),enhanced stability up to 80 h,and high direct desulfurization selectivity(>95%).This design concept is also proved to be generally applicable to modulate the slab lengths and stacking numbers of other 2D catalysts such as MoS2 and WS2 nanoflakes,which shows great potentials for developing more ultrasmall 2D catalysts with controlled sizes and excellent catalytic activities. | Xin Kang Jiancong Liu Chungui Tian Dongxu Wang Yaorui Li Hongyan Zhang Xusheng Cheng Aiping Wu Honggang Fu | 2020 | Nano Research2020，13，3： | 5 |
| 4 | Stratigraphic Sequence and Sedimentary Systems in the Middle-Southern Continental Slope of the East China Sea from Seismic Reflection Data: Exploration Prospects of Gas Hydrate显示文摘Many evidences for gas hydrate bearing sediments had been found in the continental slope of the East China Sea,such as bottom simulating reflections(BSRs),undersea gas springs,pyrite associated with methane leakage,mud diapirs/mud volcanos,bottom-water methane anomalies and so on.In this study,six key stratigraphic interfaces including T_0(seafloor),T_1(LGM,23 kyr B.P.),T_2(2.58 Myr),T_3(5.33 Myr),T_4(11.02 Myr)and T_5(16.12 Myr)were identified,and then five third-order sequences of SQIII1 to SQIII5 were divided.However,T5 in southern continental slope is not found,which shows that the middle-northern Okinawa Trough had begun to rift in the early Miocene,earlier than the southern segment.Four system tracts including lowstand systems tract(LST),transgressive systems tract(TST),highstand systems tract(HST)and falling stage systems tract(FSST)are further divided.The marine erosion interface of 11.02 Myr and regressive unconformity interface of 23 kyr B.P.indicate two large-scale sea level drop events in the research area.Seven typical seismic facies identified in the continental slope are continental shelf-edge deltas,littoral fluvial-delta plains,incised channels or submarine canyons,slope fans,submarine fans or coastal sandbars,littoral-neritic finegrained sediments,mud volcanos and some other geological bodies respectively.The minimum water depth for hydrate occurrence in the Okinawa Trough is 630 m,and the thickness of gas hydrate stability zone in continental slope is between 0 and 590 m.The calculated bottom boundary of hydrate stability zone is slightly deeper than BSRs on the seismic sections.The re-depositional turbidite sand bodies,such as canyon channels,slope fans and submarine fans developed in Quaternary strata,are the predominant hydrate reservoirs.According to developing process,the dynamic accumulation of hydrate systems can be divided into three evolutionary stages including canyon erosion and hydrate stability zone migration stage,sediments destabilizing and methane leakage stage,and channel filling and hydrate re-occurrence stage. | LI Deyong CHEN Hongyan XU Shujuan XING Junhui CHENG Honggang WANG Jinkai | 2019 | Journal of Ocean University of China2019，18，6： | 1 |
| 5 | Calculation of the residual stress of a 45 steel cylinder with a non-linear surface heat-transfer coefficient including phase transformation during quenching 显示文摘 | Cheng Heming Huang Xieqing Wang Honggang | 1999 | Journal of Materials Processing Technology1999，，8990： | 1 |
| 6 | Preparation and characteristics of tryptophan-imprinted Fe3O4/P(TRIM) composite microspheres with magnetic susceptibility by inverse emulsion-suspension polymerization显示文摘 | Lu Shulai Cheng Guoxiang Zhang Honggang | 2006 | Journal of Applied Polymer Science2006，99，6： | 1 |
| 7 | Preparation and characteristics of tryptophan-imprinted Fe304/P(TRIM) composite microspheres with magnetic susceptibility by inverse emulsion-suspension polymerization显示文摘 | Lu Shulai Cheng Guoxiang Zhang Honggang | 2006 | Journal of Applied Poly mer Science2006，99，6： | 1 |
| 8 | Preparation and characteristics of tryptophan-imprinted Fe304/p(trim)composite microspheres with magnetic susceptibility by inverse emulsion-suspension polymeri- zation 显示文摘 | Lu Shulai Cheng Guoxiang Zhang Honggang | 2006 | Journal of Applied Polymer Science2006，99，6： | 1 |
| 9 | Hydrocarbon accumulation characteristics in basement reservoirs and exploration targets of deep basement reservoirs in onshore China显示文摘Based on the global basement reservoir database and the dissection of basement reservoirs in China,the characteristics of hydrocarbon accumulation in basement reservoirs are analyzed,and the favorable conditions for hydrocarbon accumulation in deep basement reservoirs are investigated to highlight the exploration targets.The discovered basement reservoirs worldwide are mainly buried in the Archean and Precambrian granitic and metamorphic formations with depths less than 4500 m,and the relatively large reservoirs have been found in rift,back-arc and foreland basins in tectonic active zones of the Meso-Cenozoic plates.The hydrocarbon accumulation in basement reservoirs exhibits the characteristics in three aspects.First,the porous-fractured reservoirs with low porosity and ultra-low permeability are dominant,where extensive hydrocarbon accumulation occurred during the weathering denudation and later tectonic reworking of the basin basement.High resistance to compaction allows the physical properties of these highly heterogeneous reservoirs to be independent of the buried depth.Second,the hydrocarbons were sourced from the formations outside the basement.The source-reservoir assemblages are divided into contacted source rock-basement and separated source rock-basement patterns.Third,the abnormal high pressure in the source rock and the normal–low pressure in the basement reservoirs cause a large pressure difference between the source rock and the reservoirs,which is conducive to the pumping effect of hydrocarbons in the deep basement.The deep basement prospects are mainly evaluated by the factors such as tectonic activity of basement,source-reservoir combination,development of large deep faults(especially strike-slip faults),and regional seals.The Precambrian crystalline basements at the margin of the intracontinental rifts in cratonic basins,as well as the Paleozoic folded basements and the Meso-Cenozoic fault-block basements adjacent to the hydrocarbon generation depressions,have favorable conditions for hydrocarbon accumulation,and thus they are considered as the main targets for future exploration of deep basement reservoirs. | WANG Zecheng JIANG Qingchun WANG Jufeng LONG Guohui CHENG Honggang SHI Yizuo SUN Qisen JIANG Hua ABULIMITI Yiming CAO Zhenglin XU Yang LU Jiamin HUANG Linjun | 2024 | Petroleum Exploration and Development2024，51，1： | 0 |
| 10 | Identifi cation and functional analysis of phosphorylation residues of the Arabidopsis BOTRYTIS-INDUCED KINASE1显示文摘Arabidopsis BOTRYTIS-INDUCED KINASE1(BIK1)is a receptor-like cytoplasmic kinase acting early in multiple signaling pathways important for plant growth and innate immunity.It is known to form a signaling complex with a cell-surface receptor FLS2 and a co-receptor kinase BAK1 to transduce signals upon perception of pathogen-asso-ciated molecular patterns(PAMPs).Although site-specifi c phosphorylation is speculated to mediate the activation and function of BIK1,few studies have been devoted to complete profiling of BIK1 phosphorylation residues.Here,we identified nineteen in vitro autophosphoryla-tion sites of BIK1 including three phosphotyrosine sites,thereby proving BIK1 is a dual-specifi city kinase for the fi rst time.The kinase activity of BIK1 substitution mutants were explicitly assessed using quantitative mass spec-trometry(MS).Thr-237,Thr-242 and Tyr-250 were found to most signifi cantly affect BIK1 activity in autophosphoryla-tion and phosphorylation of BAK1 in vitro.A structural model of BIK1 was built to further illustrate the molecular functions of specifi c phosphorylation residues.We also mapped new sites of FLS2 phosphorylation by BIK1,which are different from those by BAK1.These in vitro results could provide new hypotheses for more in-depth in vivo studies leading to deeper understanding of how phosphorylation contributes to BIK1 activation and medi-ates downstream signaling specifi city. | Jinhua Xu Xiaochao Wei Limin Yan Dan Liu Yuanyuan Ma Yu Guo Chune Peng Honggang Zhou Cheng Yang Zhiyong Lou Wenqing Shui | 2013 | Protein & Cell2013，4，10： | 0 |
| 11 | Precise Functionalization of Remote C-H Bonds显示文摘Remote C-H bond functionalization of arenes with precise control is a recognized extraordinary challenge in organic synthesis.Recently.Yu and Houk el al.developed an elegant strategy to distinguish and functionalize remote Csp2-H bonds of(hetero)arenes within one-bond distance by the interplay of a remote directing template and a transient norbornene-type mediator.A wide range of medicinally important benzoazines are well compatible with this method The chemistry significantly expands the toolbox for site-selective functionalization of remote Co2-H bond of(hetero)arenes.This work has been published in Nature Chemistry in March,2020. | LIU Zeshui CHENG Honggang ZHOU Qianghui | 2020 | Chemical Research in Chinese Universities2020，36，4： | 0 |
| 12 | Probing surface structure on two-dimensional metal-organic layers to understand suppressed interlayer packing显示文摘Two-dimensional metal-organic layers(MOLs)from alternatively connected benzene-tribenzoate ligands and Zr6(μ3-O)_(4)(μ3-OH)_(4) or Hf6(μ3-O)_(4)(μ3-OH)_(4) secondary building units can be prepared in gram scale via solvothermal synthesis.However,the reason why the monolayers did not pack to form thick crystals is unknown.Here we investigated the surface structure of the MOLs by a combination of sum-frequency generation spectroscopy,nanoscale infrared microscopy,atomic force microscopy,aberration-corrected transmission electron microscopy,and compositional analysis.We found a partial coverage of the monolayer surface by dangling tricarboxylate ligands,which prevent packing of the monolayers.This finding illustrates low-density surface modification as a strategy to prepare new two-dimensional materials with a high percentage of exposed surface. | Peican Chen Yi Liu Xuefu Hu Xiaolin Liu En-Ming You Xudong Qian Jiawei Chen Liangping Xiao Lingyun Cao Xinxing Peng Zhongming Zeng Yibing Jiang Song-Yuan Ding Honggang Liao Zhaohui Wang Da Zhou Cheng Wang | 2020 | Nano Research2020，13，11： | 0 |
| 13 | Kondo resonance assisted thermoelectric transport through strongly correlated quantum dots显示文摘We theoretically studied the thermoelectric transport properties of a strongly correlated quantum dot system in the presence of the Kondo effect based on accurate numerical evaluations using the hierarchical equations of motion approach.The thermocurrent versus gate voltage shows a distinct sawtooth line-shape at high temperatures.In particular,the current changes from positive(hole charge)to negative(particle charge)in the electron number N=1 region due to the Coulomb blockade effect.However,at low temperatures,where the Kondo effect occurs,the thermocurrent’s charge polarity reverses,along with a significantly enhanced magnitude.As anticipated,the current sign can be analyzed by the occupation difference between particle and hole.Moreover,the characteristic turnover temperature can be further defined at which the influences of the Coulomb blockade and Kondo resonance are in an effective balance.Remarkably,the identified characteristic turnover temperature,as a function of the Coulomb interaction and dot-lead coupling,possessed a much higher value than the Kondo temperature.When a magnetic field is applied,a spin-polarized thermocurrent can be obtained,which could be tested in future experiments. | YongXi Cheng ZhenHua Li JianHua Wei HongGang Luo HaiQing Lin YiJing Yan | 2020 | Science China(Physics,Mechanics & Astronomy)2020，63，9： | 0 |
| 14 | Synthesis and Biological Evaluation of Quinolinone Compounds as SARS CoV 3CL^pro Inhibitors显示文摘SARS CoV 3CLpro 被知道是为对严重急性呼吸症候群(SARS ) 的治疗学的代理人的开发的一个有希望的目标。A quinolinone 化合物 1 经由虚拟屏蔽被选择,并且它是 synthetized 并且在 vitro 为酶的抑制测试了。化合物 1 显示出的有势力禁止的活动(IC50=0.44 ' | Yu Guo Ning Zhang Jian Wang Bo Sun Wei Liu Honggang Zhou Cheng Yang | 2013 | Chinese Journal of Chemistry2013，31，9： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员