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| 1 | Genome analysis of Taraxacum kok-saghyz Rodin provides new insights into rubber biosynthesis显示文摘The Russian dandelion Taraxacum kok-saghyz Rodin(TKS), a member of the Composite family and a potential alternative source of natural rubber(NR) and inulin, is an ideal model system for studying rubber biosynthesis. Here we present the draft genome of TKS, the first assembled NR-producing weed plant. The draft TKS genome assembly has a length of 1.29 Gb, containing 46 731 predicted protein-coding genes and68.56% repeats, in which the LTR-RT elements predominantly contribute to the genome enlargement. We analyzed the heterozygous regions/genes, suggesting its possible involvement in inbreeding depression.Through comparative studies between rubber-producing and non-rubber-producing plants, we found that enzymes of the mevalonate(MVA) pathway and rubber elongation might be critical for rubber biosynthesis, and several key isoforms have been isolated and shown to be predominantly expressed in the latex, indicating their crucial functions in rubber biosynthesis. Moreover, for two important families in rubber elongation, the CPT/CPTL and REF/SRPP families, diverse evolutionary tracks have been revealed. These results provide valuable resources and new insights into the mechanism of NR biosynthesis,and facilitate the development of alternative NR-producing crops. | Tao Lin Xia Xu Jue Ruan Shizhong Liu Shigang Wu Xiujuan Shao Xiaobo Wang Lin Gan Bi Qin Yushuang Yang Zhukuan Cheng Suhua Yang Zhonghua Zhang Guosheng Xiong Sanwen Huang Hong Yu Jiayang Li | 2018 | National Science Review2018，5，1： | 22 |
| 2 | Thermodynamic conditions of framework grain dissolution of clastic rocks and its application in Kela 2 gas field显示文摘Feldspar and clastic debris are the most important constituent framework grains of sedimentary clastic rocks and their chemical dissolution plays an essential role in the formation and evolution of the secondary pore in the reservoir rocks. On the basis of thermodynamic phase equilibrium, this study investigates the chemical equilibrium relationships between fluid and various plagioclase and K-feldspar in diagenesis of the sediments, particularly, the impact of temperature and fluid compositions (pH, activity of K+, Na+, Ca2+ and so on) on precipitation and dissolution equilibria of feldspars. Feldspar is extremely easily dissolved in the acid pore water with a low salinity when temperature decreases. The dissolution of anorthite end-member of plagioclase is related to the Ca content of the mineral and the fluid, higher Ca either in the mineral or in the fluid, easier dissolution of the feldspar. Moreover, the dissolution of albite end-member of plagioclase is related to Na of both the mineral and fluid, lower Na out of both the mineral and fluid, easier dissolution of the mineral. Similarly, lower-K fluid is more powerful to dissolve K-feldspar than the higher. The anorthite component of plagioclase is most easily dissolved in ground water-rock system, the albite is the secondary, and K-feldspar is the most stable. Selective dissolution of plagioclase occurs in diagenesis because of the plagioclase solid solution that is mainly composed of albite and anorthite end-members, Ca-rich part of which is preferentially dissolved by the pore water, in contrast to the Na-rich parts. Based on investigation of reservoir quality, secondary pore, dissolution structures of feldspar, clay minerals and ground water chemistry of the Kela 2 gas field of Kuqa Depression in the Tarim basin of Western China, we discovered that the secondary pore is very well developed in the highest quality section of the reservoir, and the plagioclase of which was obviously selectively dissolved, in contrast to the overgrowth of K-feldspar. Chemistry of the ground water out of the highest quality section of the reservoir is consistent with the complete dissolution field of anorthite, partial dissolution field of albite component of plagioclase, and the precipitation of K-feldspar on the temperature-logK phase equilibrium diagram drawn from this study. The thermodynamic modeling calculation gave us an insight into formation of the secondary pore within the reservoir, and the dissolution of plagioclase an important cause of the secondary pore. It is assumed that the solvent to dissolve the plagioclase frame grains originated from the origin-enriched compacted fluid acids out of the mudstone buried beneath the top of geopressured zones in the Kuqa Depression. | LAI Xingyun, YU Bingsong, CHEN Junyuan, CHEN Xiaolin, LIU Jianqing, MEI Mingxiang, JING Weiguang & CHENG Suhua School of Earth Sciences and Mineral Resources, China University of Geosciences, Beijing 100083, China Key Laboratory of Lithospheric Tectonics, Deep-level Process and Exploration of Ministry of Education, Beijing 100083, China | 2005 | Science China Earth Sciences2005，48，1： | 9 |
| 3 | Construction of an integrated map and location of a bruchid resistance gene in mung bean显示文摘Bruchid beetle(Callosobruchus chinensis) poses a serious threat to the production and storage of mung bean(Vigna radiata). Mapping bruchid resistance(Br) will provide an important basis for cloning the responsible gene(s) and elucidating its functional mechanism, and will also facilitate marker-assisted selection in mung bean breeding. Here, we report the construction of the genetic linkage groups of mung bean and mapping of the Br1 locus using an RIL population derived from a cross between Berken, a bruchid-susceptible line, and ACC41, a bruchid-resistant line. A total of 560 markers were mapped onto 11 linkage groups,with 38.0% of the markers showing distorted segregation. The lengths of the linkage groups ranged from 45.2 to 117.0 c M with a total coverage of 732.9 c M and an average interval of1.3 c M between loci. Br1 was located on LG9 between BM202(0.7 c M) and Vr2-627(1.7 c M).Based on 270 shared SSR markers, most of the linkage groups were assigned to specific chromosomes. These results should further accelerate the genetic study of this crop. | Lixia Wang Chuanshu Wu Min Zhong Dan Zhao Li Mei Honglin Chen Suhua Wang Chunji Liu Xuzhen Cheng | 2016 | The Crop Journal2016，4，5： | 9 |
| 4 | Modern-style Subduction Processes in the Archean:Evidence from the Shangyi Complex in North China Craton显示文摘太古代的海洋的外壳的三碎片在太古代的 granulite 带之间被发现了并且 Paleo 原生地 Hongqiyingzi 在诺思中国 craton 组织,它沿着 Shangyi-Chicheng 古老的差错传播了。这篇论文论述综合的地,岩石学,地球化学和古老的海洋的碎片的地球年代学证据。在 Shangyi 建筑群使吨子盐的年龄结晶的岩浆是 9 妈和 geochemical 特征建议的 2512 ± 1 充实 Nb 的暗岩可能与外壳的污染有关并且在在上 subduction 地区背景的 intra 海洋的弧形成。 | WANG Renmin WAN Yusheng CHENG Suhua FENG Yonggang | 2009 | Acta Geologica Sinica(English Edition)2009，83，3： | 6 |
| 5 | New ancient DNA sequences suggest high genetic diversity for the woolly mammoth (Mammuthus primigenius)显示文摘细胞色素 b 基因(mtDNA ) 的部分 DNA 序列成功地从从 Xiguitu 县(Yakeshi ) 收集的 Mammuthus primigenius 的迟了的更新世石块骨头被检索,内部蒙古自治区域并且从 Zhaodong,在北中国的 Heilongjiang 省的哈尔滨。二古老的 DNA 碎片(109 bp 和 124 bp ) 被可再现的实验与另外的 Elephantidae 税在二个不同实验室并且由种系发生的分析证实一。用在分开或联合的数据集的这些序列和出版数据的种系发生的分析在毛茸茸的猛犸和二头生活大象之中显示不稳定的关系, Elephas 和 Laxodonta。除了过去常尝试 Elephantidae 终端的长独立的进化的短序列征税一,我们建议高 intra 特定的差异在 Mammuthus primigeniuscrossing 存在空间、时间的范围,为 DNA 导致建筑群和分叉的基因背景到目前为止定序恢复。在绝灭的毛茸茸的猛犸的高基因的差异能解释 Elephantidae 税的明显的不稳定性一上分子的种系发生的树和罐头关于未解决的 Elephantidae 三分法调解明显的悖论。 | YANG Shujuan LAI Xulong SHI Suhua CHENG Shenghong ZHOU Xiugao YANG Xiujuan YANG Hong | 2006 | Progress in Natural Science:Materials International2006，16，4： | 5 |
| 6 | Genetic diversity assessment of a set of introduced mung bean accessions(Vigna radiata L.)显示文摘Genetic resources from other countries or regions play an important role in broadening the genetic background of local breeding varieties. Here we describe observations on the adaptability of mung bean germplasm obtained from the United States Department of Agriculture and their genetic diversity assessment using SSR markers. Several accessions were shown to be mixtures,based on their phenotypes for some characters. Most accessions were able to complete their lifecycles when grown in Beijing, China, making them ideal for crossbreeding without day length control. High diversity was revealed by the SSR markers, with an average of 4.2 alleles per locus and a PIC value of 0.650 per locus. STRUCTURE analysis divided the accessions into six groups.There was no obvious trend of accessions forming groups according to their geographical origin,owing mainly to germplasm exchange and an uneven distribution of accessions. The present results indicate that this germplasm would enrich the local gene pool, and provide information for the further use of germplasm in breeding programs. | Lixia Wang Peng Bai Xingxing Yuan Honglin Chen Suhua Wang Xin Chen Xuzhen Cheng | 2018 | The Crop Journal2018，6，2： | 3 |
| 7 | Contribution of Green Jadeite-Jade's Chroma Difference Based on CIE 1976 L^(^*)a^(^*)b(^*) Uniform Color Space显示文摘The chroma difference(DC) and lightness difference (DL) of 288 jadeite-jades with fine texture and even green color have been analyzed based on color difference formula CIE LAB in the uniform color space CIE 1976 L^~*a^~*b^~* to evaluate their contribution to the color appearance and color difference(DE).The analysis of lightness(L^~*), chroma(C^~*) and hue-angle(h_o) show that the vivid yellowish green color occurs when L^~*∈5.29-59.2, | Ying Guo~1,Tao Mo~1,Suhua Cheng~2 1.School of Gemology,China University of Geosciences(Beijing),Beijing 100083,China. 2.School of Earth Sciences and Resources,China University of Geosciences(Beijing),Beijing 100083,China | 2009 | 地学前缘2009，16，S1： | 1 |
| 8 | Genetic diversity and a population structure analysis of accessions in the Chinese cowpea [Vigna unguiculata(L.)Walp.]germplasm collection显示文摘Cowpea(Vigna imguicuiata) is an important legume crop with diverse uses. The species is presently a minor crop, and evaluation of its genetic diversity has been very limited. In this study, a total of 200 genic and 100 genomic simple sequence repeat(SSR) markers were developed from cowpea unigene and genome sequences, respectively. Among them, 27 genic and 27 genomic SSR markers were polymorphic and were used for assessment of genetic diversity and population structure in 105 selected cowpea accessions. A total of 155 alleles and 2.9 alleles per marker were identified, and the average polymorphic information content(PIC) value was 0.3615. The average PIC of genomic SSRs(0.3996) was higher than that of genic SSRs(0.3235), and most of the polymorphic genomic SSRs were composed of di-and trinucleotide repeats(51.9% and 37.0% of all loci, respectively). The low level of detected genetic diversity may be attributed to a severe genetic bottleneck that occurred during the cowpea domestication process. The accessions were classified by structure and cluster analysis into four subgroups that correlated well with their geographic origins or collection sites. The classification results were also consistent with the results from principal coordinate analysis and can be used as a guide during future germplasm collection and selection of accessions as breeding materials for cultivar improvement. The newly developed genic and genomic SSR markers described in this study will be valuable genomic resources for the assessment of genetic diversity, population structure, evaluation of germplasm accessions, construction of genetic maps, identification of genes of interest,and application of marker-assisted selection in cowpea breeding programs. | Honglin Chen Hong Chen Liangliang Hu Lixia Wang Suhua Wang Ming Li Wang Xuzhen Cheng | 2017 | The Crop Journal2017，5，5： | 1 |
| 9 | Analysis of simple sequence repeats in rice bean(Vigna umbellata) using an SSR-enriched library显示文摘Rice bean(Vigna umbellata Thunb.), a warm-season annual legume, is grown in Asia mainly for dried grain or fodder and plays an important role in human and animal nutrition because the grains are rich in protein and some essential fatty acids and minerals. With the aim of expediting the genetic improvement of rice bean, we initiated a project to develop genomic resources and tools for molecular breeding in this little-known but important crop.Here we report the construction of an SSR-enriched genomic library from DNA extracted from pooled young leaf tissues of 22 rice bean genotypes and developing SSR markers.In 433,562 reads generated by a Roche 454 GS-FLX sequencer, we identified 261,458 SSRs, of which 48.8% were of compound form. Dinucleotide repeats were predominant with an absolute proportion of 81.6%, followed by trinucleotides(17.8%). Other types together accounted for 0.6%. The motif AC/GT accounted for 77.7% of the total, followed by AAG/CTT(14.3%), and all others accounted for 12.0%. Among the flanking sequences, 2928 matched putative genes or gene models in the protein database of Arabidopsis thaliana, corresponding with 608 non-redundant Gene Ontology terms. Of these sequences, 11.2% were involved in cellular components, 24.2% were involved molecular functions, and 64.6% were associated with biological processes. Based on homolog analysis, 1595 flanking sequences were similar to mung bean and 500 to common bean genomic sequences. Comparative mapping was conducted using 350 sequences homologous to both mung bean and common bean sequences. Finally, a set of primer pairs were designed, and a validation test showed that58 of 220 new primers can be used in rice bean and 53 can be transferred to mung bean.However, only 11 were polymorphic when tested on 32 rice bean varieties. We propose that this study lays the groundwork for developing novel SSR markers and will enhance the mapping of qualitative and quantitative traits and marker-assisted selection in rice bean and other Vigna species. | Lixia Wang Kyung Do Kim Dongying Gao Honglin Chen Suhua Wang Suk Ha Lee Scott A. Jackson Xuzhen Cheng | 2016 | The Crop Journal2016，4，1： | 0 |
| 10 | Haplotype-resolved and chromosome-level genome assembly of Colorado potato beetle显示文摘As the most important non-cereal food crop,potatoes are a staple food for 1.3 billion people(Stokstad,2019).However,these cultivated potatoes are challenged with multiple biotic stresses(i.e.,pathogens and pests),some of which can evade host resistance,leading to a serious dampening of potato yield.One of these pests is the Colorado potato beetle(hereafter referred to as CPB,Leptinotarsa decemlineata Say).CPB is a member of the order Coleoptera,and originated in the southwestern United States and Mexico(Alyokhin et al.,2008). | Ziqi Ye Ruirui Lu Chao Li Doudou Yang Zhuozhen Zeng Weichao Lin Jie Cheng Zhongmin Yang Li Wang Yulin Gao Sanwen Huang Xingtan Zhang Suhua Li | 2023 | Journal of Genetics and Genomics2023，50，7： | 0 |
| 11 | Geochemical Characteristics of the Raohe Ophiolite, Heilongjiang Province, Northeast China显示文摘Located in the easternmost part of Heilongjiang Province,Northeast China,Raohe County is geologically a part of the Sikhote-Alin accretionary orogen,which is bounded by the Jimusi massif in the west,and by the Xingkai massif in the south.A sequence of basalt,diabase,gabbro and ultramafic rocks occurs in the western | WANG Yang CHENG Suhua | 2015 | Acta Geologica Sinica(English Edition)2015，89，A02： | 0 |
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