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| 1 | Transactivation of the TIEG1 confers growth inhibition of transforming growth factor-β-susceptible hepatocellular carcinoma cells显示文摘AIM:To investigate the role of transforming growth factor(TGF)-β-inducible early gene 1(TIEG1) in TGF-β-induced growth inhibition in hepatocellular carcinoma(HCC) cells.METHODS:Human hepatocyte and HCC cell lines with varied susceptibilities to TGF-β1 were tested by methylthiazoletetrazolium(MTT) assay.The expression changes of Smad2,Smad3,Smad4,Smad7,TIEG1 and TIEG2 gene following treatment with TGF-β1 in a TGF-β-sensitive hepatocyte cell line(MIHA),a TGF-β-sensitive hepatoma cell line(Hep3B) and two TGF-β-insensitive hepatoma cell lines(HepG2 and Bel7404) were examined.SiRNA targeting TIEG1 was transfected into Hep3B cells and the sensitivity of cells to TGF-β1 was examined.Overexpression of TIEG1 was induced by lentiviral-mediated transduction in TGF-β1-resistant hepatoma cell lines(Bel7404 and HepG2).MTT assay and 4',6-Diamidino-2-phenylindole staining were used to identify cell viability and apoptosis,respectively.The expression level of stathmin was measured by reverse transcriptase polymerase chain reaction and Western-blotting analysis,and stathmin promoter activity by TIEG1 was monitored by a luciferase reporter gene system.RESULTS:TIEG1 was significantly upregulated by TGF-β1 in the TGF-β1-sensitive HCC cell line,Hep3B,but not in the resistant cell lines.The suppression of TIEG1 by siRNAs decreased the sensitivity of Hep3B cells to TGF-β1,whereas the overexpression of TIEG1 mediated growth inhibition and apoptosis in TGF-β1-resistant HCC cell lines,which resembled those of TGF-β1-sensitive HCC cells treated with TGF-β1.Our data further suggested that stathmin was a direct target of TIEG1,as stathmin was signif icantly downregulated by TIEG1 overexpression,and stathmin promoter activity was inhibited by TIEG1 in a dose-dependent manner.CONCLUSION:Our data suggest that transactivation of TIEG1 conferred growth inhibition of TGF-β-susceptible human HCC cells. | Lei Jiang Yiu-Kay Lai Jin-Fang Zhang Chu-Yan Chan Gang Lu Marie CM Lin Ming-Liang He Ji-Cheng Li Hsiang-Fu Kung | 2012 | World Journal of Gastroenterology2012，18，17： | 13 |
| 2 | Islet separation and islet cell culture in vitro from human embryo-pancreas显示文摘 | Yuan Z Wu GY He YS Shao CM Zhan Y | 1999 | World Journal of Gastroenterology1999，5，5： | 3 |
| 3 | Relation between intensity of physical activity and breast cancer risk reduction显示文摘 | Friedenreich CM Courneya KS Bryant HE | 2001 | Med Sic Sports Exerc2001，33，9： | 2 |
| 4 | Association of the angiotensin converting enzyme gene IrD polymorphism with change in left ventricular mass in response to physical training显示文摘 | Montgomery HE Clarkson P Dollery CM | 1997 | Circulation1997，96，： | 1 |
| 5 | DDB1 functions as a linker to recruit receptor WD40 proteins to CUL4-ROC1 ubiquitin ligases 显示文摘 | He YJ McCall CM Hu J | 2006 | Genes Dev2006，20，： | 1 |
| 6 | Clay minerals in a soil chronosequence derived from basalt on Hainan Island, China and its implication for pedogenesis显示文摘 | He Y Li DC Velde B Yang YF Huang CM Gong ZT Zhang GL | 2008 | Geoderma2008，148，： | 1 |
| 7 | Rapid mobilization of murine and human hematopoietic stem and progenitor cells with AMD3100, a CXCR4 antagonist显示文摘 | Broxmeyer HE Orschell CM Clapp DW | 2005 | J Exp Med2005，8，： | 1 |
| 8 | Study on the expression of an- giotensin II (ANG II) receptor subtype 1 (AT1R) in the placenta of pregnancy-induced hypertension 显示文摘 | Thapa L He CM Chen HP | 2004 | Placenta2004，25，7： | 1 |
| 9 | Rapid mobilization of murine and human hematopoietic stem and progenitor cells with AMD3100, a CXCR4 antagonist 显示文摘 | Broxmeyer HE Orschell CM Clapp DW | 2005 | J Exp Med2005，201，8： | 1 |
| 10 | Bone morphogenetic proteins in craniofacial surgery: current techniques, clinical experiences,and the future of personalized stem cell therapy 显示文摘 | Chenard KE Teven CM He TC | 2012 | J Biomed Biotechnol2012，2012，60： | 1 |
| 11 | Fcols Rush in? The Institutional Context of Industry Creation 显示文摘 | Aldrich HE Fiol CM | 1994 | Academy of Management Re- view1994，19，04： | 1 |
| 12 | MiR-125b expression affects the proliferation and apoptosis of human glioma cells by targeting Bmf显示文摘 | Xia HF He TZ Liu CM | 2009 | Cell Physiol Biochem2009，23，46： | 1 |
| 13 | Post - transplant lymphopro- liferative disorders 显示文摘 | Cottschalk S Rooney CM Heslop HE | 2005 | Ann Rev Med2005，56，： | 1 |
| 14 | Regulation of opioid toler- ance by let-7 family microRNA targeting the mu opioid receptor 显示文摘 | He Y Yang C Kirkmire CM | 2010 | J Neurosci2010，30，10： | 1 |
| 15 | Maternal oul- comes in pregnancies complicated by obesity 显示文摘 | Robinson HE O'Connell CM Joseph KS | 2005 | Obstet Gynecol2005，106，6： | 1 |
| 16 | Rapid mobilization of murine and human hematopoietic stem and progenitor cells with AMD3100, a CXCR4 antagonist 显示文摘 | Broxmeyer HE Orschell CM Clapp DW | 2005 | J Exp Med2005，201，8： | 1 |
| 17 | MiR-125b expression affects the proliferation and apoptosis of human glioma cells by targeting Bmf 显示文摘 | Xia I-IF He TZ Liu CM | 2009 | Cell Physiol Biochem2009，23，： | 1 |
| 18 | Study on the expression of angiotension Ⅱ(ANGII) recept subtype 1 (AT1R) in the placenta of pregnancy induced hypertension显示文摘 | Thapa L He CM Chen HP | 2004 | Clin Sci2004，25，7： | 1 |
| 19 | Rapid mobiliza- tion of murine and human hematopoietic stem and progenitor cellswith AMD3100, a CXCR4 antagonist显示文摘 | Broxmeyer HE Orschell CM Clapp DW | 2005 | J Exp Med2005，201，8： | 1 |
| 20 | Consequences of hip fracture on activities of daily life and residential needs 显示文摘 | Osnes EK Lofthus CM Meyer HE | 2004 | Osteoporosi int2004，15，： | 1 |
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